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Numerous analytical approaches have been developed to determine histamine levels in food samples due to its health consequences. Consuming histamine over the Food and Drug Administration (FDA)-regulated 50 mg kg-1 limit would result in chronic toxicity. Consequently, the present study discusses a novel electrochemical approach to evaluate histamine levels in fish products via a molecularly imprinted polymer (MIP) on an electrode surface. The film was produced with electropolymerized polyurethane (PU), which maintained the histamine compound. Fourier-transform infrared (FTIR) spectroscopy was applied to verify the MIP manufactured in this study. The capability of the polymer was measured by assessing its electron shifts with cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Differential pulse voltammetry (DPV) was also employed to validate the sensing method. The MIP/screen-printed electrode (SPE) and non-imprinted polymer (NIP)/SPE recorded a linear response ranging from 1 to 1000 nmol L-1 at the 1.765 and 709 nmol L-1 detection limits. The sensing technique was subsequently utilized to determine the histamine levels in selected samples at room temperature (25 °C). Generally, the sensor allowed the accurate and precise detection of histamine in the fish samples. Furthermore, the approach could be categorized as a simple technique that is low-cost and suitable for on-site detections.
RESUMO
INTRODUCTION: Stunting continues to be a prominent health problem in Indonesia. Stunting prevalence is higher in children from poor families or living in rural areas; however, there has been a lack of information on predictors of stunting disparities and stunting risk factors by socioeconomic status (SES) and location of residence (rural or urban). This study aims to examine the factors associated with stunting by SES and rural-urban status, and to assess determinants of stunting disparities by SES and rural-urban status in Indonesia. METHODS: This study analysed data from the Indonesian Family and Life Survey (IFLS) wave 5, conducted in 2014. Data for 3887 children aged 0-59 months, including individual, family and community variables, were analysed. Stratified logistic regression was conducted to assess differences in determinants of stunting by household expenditure (poor or non-poor, representing SES) and rural-urban status. The Oaxaca-Blinder decomposition method was used to assess determinants of stunting disparities by household expenditure and rural-urban status. RESULTS: The analyses showed differences in factors associated with stunting among children in rural versus urban areas, or children in poor versus non-poor households. Mother's short stature and low education level increased the odds of stunting across all groups. However, in children of families with a higher household expenditure, unhealthy snacks were a significant predictor of stunting (adjusted odds ratio (aOR) 1.23, 95% confidence interval (CI) 1.04-1.47). This finding was not found in other groups. Good sanitation significantly reduced stunting in children in families with higher household expenditure and children from urban communities. Nutrition services were significantly associated with stunting in poor children and children from urban areas. The decomposition analyses showed that differences in characteristics explained 55.35% stunting disparity by household expenditure. Meanwhile, rural-urban disparity was mostly explained by differences in responses (56.20%), with low birth weight and unexplained variables as predominant contributors. CONCLUSION: There were slight differences in stunting determinants by household expenditure and rural-urban status in Indonesia. Stunting disparities were attributed to differences in characteristics and responses between the less and more advantaged populations. To improve the effectiveness of stunting reduction programs, specific interventions tailored to address the differences between the more and less advantaged population are needed.