A genetic history of the Balkans from Roman frontier to Slavic migrations.

The rise and fall of the Roman Empire was a socio-political process with enormous ramifications for human history. The Middle Danube was a crucial frontier and a crossroads for population and cultural movement. Here, we present genome-wide data from 136 Balkan individuals dated to the 1st millennium CE. Despite extensive militarization and cultural influence, we find little ancestry contribution from peoples of Italic descent. However, we trace a large-scale influx of people of Anatolian ancestry during the Imperial period. Between ∼250 and 550 CE, we detect migrants with ancestry from Central/Northern Europe and the Steppe, confirming that "barbarian" migrations were propelled by ethnically diverse confederations. Following the end of Roman control, we detect the large-scale arrival of individuals who were genetically similar to modern Eastern European Slavic-speaking populations, who contributed 30%-60% of the ancestry of Balkan people, representing one of the largest permanent demographic changes anywhere in Europe during the Migration Period.

Early dispersal of domestic horses into the Great Plains and northern Rockies.

The horse is central to many Indigenous cultures across the American Southwest and the Great Plains. However, when and how horses were first integrated into Indigenous lifeways remain contentious, with extant models derived largely from colonial records. We conducted an interdisciplinary study of an assemblage of historic archaeological horse remains, integrating genomic, isotopic, radiocarbon, and paleopathological evidence. Archaeological and modern North American horses show strong Iberian genetic affinities, with later influx from British sources, but no Viking proximity. Horses rapidly spread from the south into the northern Rockies and central plains by the first half of the 17th century CE, likely through Indigenous exchange networks. They were deeply integrated into Indigenous societies before the arrival of 18th-century European observers, as reflected in herd management, ceremonial practices, and culture.

Waist to height ratio is the anthropometric index that most appropriately mirrors the lifestyle and psychological risk factors of obesity.

AIM: To determine to what extent the lifestyle and psychological factors influenced the occurrence of obesity in the population of the Mediterranean island of Vis (Croatia); also to relate the examined risk factors to several obesity indicators in order to select the most sensitive one. METHODS: Cross-sectional population study included 960 participants of both sexes (18-93 years) in whom the obesity was estimated using anthropometric indicators. The factor analysis of 55-item Food Frequency Questionnaire (FFQ) was performed as well as the General Health Questionnaire (GHQ12) for the self-assessment of psychological health and wellbeing. FFQ and GHQ12 individual factors' scores, sex, age, physical activity, smoking and socioeconomic status were further used as independent variables in the multiple linear regression analyses with five obesity indicators as dependent variables. RESULTS: The examined risk factors explained the highest proportion of variance of the waist to height ratio (WHtR) in comparison with all other indicators of obesity (body mass index (BMI), waist to hip ratio, sum of skinfolds thicknesses, fat mass percentage). Only the dietary factors showed a significant relation to BMI, while almost all of the tested variables-dietary pattern, psychological structure, smoking habit and physical activity-had a significant association with WHtR. CONCLUSIONS: WHtR is the most useful indicator of obesity as it had a profiled relation with a number of lifestyle factors as well as with psychological health. The present study also revealed the importance of psychological factors for obesity phenotype, particularly the behavioural pattern which could be described as avoidance of dealing with problems.

Hide and seek: Impacted maxillary and mandibular canines from the Roman period Croatia.

OBJECTIVE: The aim of this paper was to analyse the dental remains of an adult male with three impacted canines from the Roman period cemetery in Osijek, Croatia. MATERIALS: The dental remains of an adult male aged 35-45 years at the time of death were analysed. METHODS: Macroscopic analysis of dental remains was accompanied by radiographic examination. RESULTS: The individual also had additional dental pathologies (carious lesion, antemortem tooth loss). A total of 21 permanent teeth were present in maxillae and mandible. Eighteen of them were normal fully erupted dentition, while three were impacted: left maxillary canine and both mandibular canines. The left maxillary and mandibular canines were mesially inclined, and the right mandibular canine was relatively horizontally positioned. CONCLUSION: A case of non-syndrome impaction in which the lack of eruptive force in combination with the unfavorable position of the tooth bud might have resulted in multiple impacted teeth is presented. SIGNIFICANCE: Current clinical knowledge reports low frequency of this anomaly, with maxillary impaction occurring more often than mandibular. Furthermore, canine impaction is even more rarely reported in the archaeological material. The presented case is the only one from the archaeological setting with impaction present both in maxillae and mandible. LIMITATIONS: In modern populations multiple impactions are often associated with various syndromes. Since the majority of syndromes affect soft tissue, their association with impaction cannot be confirmed in archaeological populations.

Rib anomalies in a Neolithic period skeleton from Croatia.

ABSTRACT: Congenital anomalies develop during intrauterine development. They can occur in soft tissues as well as skeletal elements and vary from minor to life threatening. In this paper we present a case of an individual from the Neolithic site of Vinka, Croatia. This adult male exhibited multiple congenital anomalies on cranial and postcranial skeleton. These include several rib anomalies on the right side: hyperplasia of the third rib, bifid fourth rib and flaring of the eighth rib. Additionally, lambdoid ossicles, parastyle of the second maxillary molar and supracondylar processes of the humeri are present. In this study we put emphasis on rib anomalies, very rarely reported in archaeological settings, and compare them to clinical cases.

Dental stigmata and enamel thickness in a probable case of congenital syphilis from XVI century Croatia.

OBJECTIVE: To analyse the dental remains of an individual with signs of congenital syphilis by using macroscopic observation, CBCT and micro-CT images, and the analysis of the enamel thickness. DESIGN: Anthropological analysis of human skeletal remains from the 16th century archaeological site Park Gric in Zagreb, Croatia discovered a female, 17-20 years old at the time of death, with dental signs supportive of congenital syphilis: mulberry molars and canine defects, as well as non-specific hypoplastic changes on incisors. The focus of the analysis was on three aspects: gross morphology, hypoplastic defects of the molars, canines and incisors, as well as enamel thickness of the upper first and second molars. RESULTS: The observed morphology of the first molars corresponds to the typical aspect of mulberry molars, while that of the canines is characterised by hypomineralisation. Hypoplastic grooves were observed on the incisal edges of all incisors. The enamel of the first molars is underdeveloped while in the second molars a thick-enamelled condition is observed. CONCLUSIONS: Our observations for the dental and skeletal evidence are supportive to a diagnosis of congenital syphilis for this specimen from XVI century Croatia. The use of CT imaging helped documenting the diagnostic features and quantifying the effect of the dental stigmata on first molars.

Frontal sinus osteoma in a 16th century skeleton from Zagreb, Croatia.

The analysis of 16th century graves from Zagreb, Croatia, revealed a case of frontal sinus osteoma in a middle-aged female. This lesion was discovered during visual examination, due to postmortem breakage of the frontal bone. The significance of this finding is based on the fact that frontal sinus osteomas are very rarely reported in the palaeopathological literature, despite the fact that they account for 80% of all paranasal sinus osteomas in modern populations. This paper presents results of macroscopic and radiographic analyses of the lesion, accompanied by a detailed differential diagnosis. Although tumours are commonly considered diseases of modern lifestyles, the described case confirms their occurrence in the past.

Autoimmune joint diseases in Late Medieval skeletal sample from Croatia.

Analysis of 25 skeletons from Late Medieval cemetery Uzdolje-Grablje near Knin, Croatia, revealed three cases of systematic pathological changes to joints. Observed pathological lesions were examined macroscopically and radiologically and compared to the available paleopathological standards in order to formulate a differential diagnosis. In all three cases observed changes were most consistent with autoimmune joint diseases including ankylosing spondylitis, juvenile idiopathic arthritis and psoriatic arthritis. Based on published clinical studies, we suggest that the high prevalence of autoimmune diseases in our skeletal sample stems from the genetic basis of the autoimmunity, and that three individuals describe here are possibly closely related.