Foreign Pests as Potential Threats to North American Apiculture: Tropilaelaps mercedesae, Euvarroa spp, Vespa mandarinia, and Vespa velutina.

Honey bees face a broad range of threats globally. Many of these threats originate outside of North America because honey bees are an introduced species. Invasive pests are among the most widely distributed, damaging, and economically costly honey bee hive associates. As international trade and travel continue at a rapid pace, the list of invasive apicultural pests likely will grow. Details of these organisms' life history relevant to management and eradication efforts are addressed. Methods and proposed methods of detection and management encountered abroad are discussed.

Varroa destructor feeds primarily on honey bee fat body tissue and not hemolymph.

The parasitic mite Varroa destructor is the greatest single driver of the global honey bee health decline. Better understanding of the association of this parasite and its host is critical to developing sustainable management practices. Our work shows that this parasite is not consuming hemolymph, as has been the accepted view, but damages host bees by consuming fat body, a tissue roughly analogous to the mammalian liver. Both hemolymph and fat body in honey bees were marked with fluorescent biostains. The fluorescence profile in the guts of mites allowed to feed on these bees was very different from that of the hemolymph of the host bee but consistently matched the fluorescence profile unique to the fat body. Via transmission electron microscopy, we observed externally digested fat body tissue in the wounds of parasitized bees. Mites in their reproductive phase were then fed a diet composed of one or both tissues. Mites fed hemolymph showed fitness metrics no different from the starved control. Mites fed fat body survived longer and produced more eggs than those fed hemolymph, suggesting that fat body is integral to their diet when feeding on brood as well. Collectively, these findings strongly suggest that Varroa are exploiting the fat body as their primary source of sustenance: a tissue integral to proper immune function, pesticide detoxification, overwinter survival, and several other essential processes in healthy bees. These findings underscore a need to revisit our understanding of this parasite and its impacts, both direct and indirect, on honey bee health.