Protein changes during ethanol induced seed germination in Aconitum heterophyllum.

Aconitum heterophyllum is a high altitude medicinal plant that has become endangered due to overexploitation for their aconitins. The most effective, conventional propagation method for any plant species is by seed. However, in Aconitum seed germination is erratic, and seedling survival is low. In the present study results have been discussed on the possible implication of ethanol treatment on removal of barriers on radical emergence in terms of protein changes. Eighty seven percent of seed germination was achieved in Aconitum with ethanol treatment. Comparative 2-DE analysis of ethanol treated and untreated seed protein profiles in Phase II of germination revealed 40 differentially expressed proteins. Twenty-seven out of 40 proteins were induced, 5 were increased and 8 were repressed. Mass spectrometry and subsequent identification confirmed that these proteins were involved in metabolism, DNA regulation, stress tolerance and plasmamembrane/cell wall biosynthesis/extension processes. These protein changes might be responsible for physiological and physical changes, respectively, resulted in increase in germination percentage. Further, characterization of these proteins will be of great help in understanding the molecular mechanism lying behind enhanced germination in response to ethanol treatment.

Optimization of processing conditions for cyanide content reduction in fresh bamboo shoot during NaCl treatment by response surface methodology.

NaCl treatment was optimized for maximum reduction of cyanide content in raw bamboo (Dendrocalamus strictus) shoot using response surface methodology with 4 independent variables like concentration of NaCl, thickness of bamboo shoot, amount of NaCl solution and duration of treatment at 3 levels of each variable. Box-Behnken design was used to select the levels of variables in experimental runs. Cyanide content ranged between 12.8 and 29.6 mg/kg in bamboo shoot after NaCl treatment. The effect of concentration of NaCl was higher in reducing the cyanide content followed by thickness of bamboo shoot and treatment time in decreasing order. Amount of NaCl solution did not affect the reduction of cyanide content significantly. Optimum condition was 2.4% NaCl concentration, 1.25 cm thickness of bamboo shoot, 216 ml of NaCl solution and 23 min treatment. Corresponding cyanide content was 11.2 mg/kg. Experimental verification at optimum condition gave average cyanide content of 11.3 mg/kg on fresh weight basis, which was in good agreement with predicted and was well below permissible limit (500 mg/kg). The reduction of cyanide content was of 98.3% at optimum level.