KIAA0040 enhances glioma growth by controlling the JAK2/STAT3 signalling pathway.

The role of KIAA0040 role in glioma development is not yet understood despite its connection to nervous system diseases. In this study, KIAA0040 expression levels were evaluated using qRT-PCR, WB and IHC, and functional assays were conducted to assess its impact on glioma progression, along with animal experiments. Moreover, WB was used to examine the impact of KIAA0040 on the JAK2/STAT3 signalling pathway. Our study found that KIAA0040 was increased in glioma and linked to tumour grade and poor clinical outcomes, serving as an independent prognostic factor. Functional assays showed that KIAA0040 enhances glioma growth, migration and invasion by activating the JAK2/STAT3 pathway. Of course, KIAA0040 enhances glioma growth by preventing tumour cell death and promoting cell cycle advancement. Our findings suggest that targeting KIAA0040 could be an effective treatment for glioma due to its role in promoting aggressive tumour behaviour and poor prognosis.

Divergence of 10 satellite repeats in Artemisia (Asteraceae: Anthemideae) based on sequential fluorescence in situ hybridization analysis: evidence for species identification and evolution.

Artemisia is a large genus encompassing about 400 diverse species, many of which have considerable medicinal and ecological value. However, complex morphological information and variation in ploidy level and nuclear DNA content have presented challenges for evolution studies of this genus. Consequently, taxonomic inconsistencies within the genus persist, hindering the utilization of such large plant resources. Researchers have utilized satellite DNAs to aid in chromosome identification, species classification, and evolutionary studies due to their significant sequence and copy number variation between species and close relatives. In the present study, the RepeatExplorer2 pipeline was utilized to identify 10 satellite DNAs from three species (Artemisia annua, Artemisia vulgaris, Artemisia viridisquama), and fluorescence in situ hybridization confirmed their distribution on chromosomes in 24 species, including 19 Artemisia species with 5 outgroup species from Ajania and Chrysanthemum. Signals of satellite DNAs exhibited substantial differences between species. We obtained one genus-specific satellite from the sequences. Additionally, molecular cytogenetic maps were constructed for Artemisia vulgaris, Artemisia leucophylla, and Artemisia viridisquama. One species (Artemisia verbenacea) showed a FISH distribution pattern suggestive of an allotriploid origin. Heteromorphic FISH signals between homologous chromosomes in Artemisia plants were observed at a high level. Additionally, the relative relationships between species were discussed by comparing ideograms. The results of the present study provide new insights into the accurate identification and taxonomy of the Artemisia genus using molecular cytological methods.

Brain structures associated with individual differences in decisional and emotional forgiveness.

In responding to interpersonal conflicts, forgiveness goes a long way. Past brain imaging studies have examined the activation patterns of forgiving responses. However, the individual differences in brain structures associated with decisional forgiveness and emotional forgiveness are not well understood. In this voxel-based morphometry study, participants (85 men, 210 women) completed the Decisional Forgiveness Scale (DFS) and the Emotional Forgiveness Scale (EFS) and underwent an anatomical magnetic resonance imaging scan. Higher DFS scores were associated with larger GM volumes in a cluster that included regions in the orbitofrontal cortex (OFC). Higher EFS scores were associated with larger GM volumes in a cluster that included regions in the medial prefrontal cortex (mPFC) and the superior frontal gyrus (SFG), which were also associated with smaller GM volumes in a cluster that included regions in the left inferior parietal lobule (IPL). The associations between the identified regions and DFS scores and EFS scores were supported by the cross-validation test. In addition, the GMV of OFC, mPFC and SFG partially mediated the relationship between DFS and EFS. These results provide direct neuroanatomical evidence for an association between decisional and emotional forgiveness and brain regions which are critical for cognitive control, theory of mind and moral judgment.