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Mol Cell ; 84(5): 981-989.e7, 2024 Mar 07.
Artigo em Inglês | MEDLINE | ID: mdl-38295803

RESUMO

Coenzyme Q (CoQ) is a redox lipid that fulfills critical functions in cellular bioenergetics and homeostasis. CoQ is synthesized by a multi-step pathway that involves several COQ proteins. Two steps of the eukaryotic pathway, the decarboxylation and hydroxylation of position C1, have remained uncharacterized. Here, we provide evidence that these two reactions occur in a single oxidative decarboxylation step catalyzed by COQ4. We demonstrate that COQ4 complements an Escherichia coli strain deficient for C1 decarboxylation and hydroxylation and that COQ4 displays oxidative decarboxylation activity in the non-CoQ producer Corynebacterium glutamicum. Overall, our results substantiate that COQ4 contributes to CoQ biosynthesis, not only via its previously proposed structural role but also via the oxidative decarboxylation of CoQ precursors. These findings fill a major gap in the knowledge of eukaryotic CoQ biosynthesis and shed light on the pathophysiology of human primary CoQ deficiency due to COQ4 mutations.


Assuntos
Células Eucarióticas , Ubiquinona , Humanos , Descarboxilação , Células Eucarióticas/metabolismo , Oxirredução , Escherichia coli/genética , Escherichia coli/metabolismo , Estresse Oxidativo , Proteínas Mitocondriais/metabolismo
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