Molecular typing of strains of staphylococcus aureus isolated from bone and joint lesions in lame broilers by random amplification of polymorphic DNA.

A study was conducted to investigate the relationship between the presence of localized bacterial infection and lameness in broiler fowl (Gallus gallus domesticus). Isolation of bacteria from the proximal femur, proximal tibia, and tibiotarsus from broilers with lameness revealed a probable association between lameness and the presence of Staphlylococcus aureus. Other potential pathogens, including Escherichia coli and DNase-negative staphylococci, were also isolated from sound and lame birds, and their association with pathologies causing lameness was less well defined. After trials with a set of twenty 10-base oligonucleotide primers, a pair of primers giving optimal performance was selected. The S. aureus isolates were typed by random amplification of polymorphic DNA (RAPD) by using the pair of 10-mer primers, and groupings were defined by banding patterns after agarose gel electrophoresis. The putative RAPD groupings may provide a basis for epidemiological studies of S. aureus in broiler production systems.

Mucosal immunization with experimental feline immunodeficiency virus (FIV) vaccines induces both antibody and T cell responses but does not protect against rectal FIV challenge.

Feline immunodeficiency virus (FIV) is a natural lentiviral pathogen of cats which can be experimentally transmitted via rectal and vaginal routes--the major routes of human immunodeficiency virus type 1 transmission in man. An important objective for lentiviral research is the development of vaccine strategies which generate good mucosal immune responses capable of giving protection from a mucosal virus challenge. The experimental vaccines employed in this study were based on (a) a peptide from the third variable region of the FIV envelope glycoprotein and (b) fixed whole FIV, Glasgow-8 strain. Adjuvants used were Quil A and cholera toxin for mucosal administration and incomplete Freund's adjuvant and immune stimulating complexes for subcutaneous injection. Mucosal immunization was given by rectal and intranasal routes. Both antibody and proliferative responses were elicited by mucosal immunization and cholera toxin was found to be a good mucosal adjuvant. The addition of a lipo thioester to the FIV peptide improved IgG and IgA responses upon parenteral administration. However, no protection from a rectal FIV challenge was achieved.

Natural Borna disease virus infection in cats in the United Kingdom.

Borna disease virus (BDV) is a novel RNA virus that has only recently been characterised and classified in a new virus family, Bornaviridae. The virus was detected in buffy coat cells from four of five cats with neurological disease and in the brains of five of 15 cats with nervous signs and of one of three cats with non-neurological disease. In a serosurvey of 111 cats the incidence of antibody to BDV in cats with neurological disease was higher than in cats with other types of disease, suggesting that the virus may play a role in nervous diseases of cats in the UK.