RESUMO
The feline leukemia virus (FeLV) belongs to the Retroviridae family and Gammaretrovirus genus, and causes a variety of neoplastic and non-neoplastic diseases in domestic cats (Felis catus), such as thymic and multicentric lymphomas, myelodysplastic syndromes, acute myeloid leukemia, aplastic anemia, and immunodeficiency. The aim of the present study was to carry out the molecular characterization of FeLV-positive samples and determine the circulating viral subtype in the city of São Luís, Maranhão, Brazil, as well as identify its phylogenetic relationship and genetic diversity. The FIV Ac/FeLV Ag Test Kit (Alere™) and the commercial immunoenzymatic assay kit (Alere™) were used to detect the positive samples, which were subsequently confirmed by ELISA (ELISA - SNAP® Combo FeLV/FIV). To confirm the presence of proviral DNA, a polymerase chain reaction (PCR) was performed to amplify the target fragments of 450, 235, and 166 bp of the FeLV gag gene. For the detection of FeLV subtypes, nested PCR was performed for FeLV-A, B, and C, with amplification of 2350-, 1072-, 866-, and 1755-bp fragments for the FeLV env gene. The results obtained by nested PCR showed that the four positive samples amplified the A and B subtypes. The C subtype was not amplified. There was an AB combination but no ABC combination. Phylogenetic analysis revealed similarities (78% bootstrap) between the subtype circulating in Brazil and FeLV-AB and with the subtypes of Eastern Asia (Japan) and Southeast Asia (Malaysia), demonstrating that this subtype possesses high genetic variability and a differentiated genotype.
Assuntos
Doenças do Gato , Vírus da Imunodeficiência Felina , Gatos , Animais , Vírus da Leucemia Felina/genética , Brasil , Filogenia , Genótipo , Reação em Cadeia da Polimerase/veterinária , Vírus da Imunodeficiência Felina/genéticaRESUMO
O objetivo foi verificar o perfil de paciente atendido e realizar o dimensionamento da equipe de Enfermagem na UTI de um hospital filantrópico. É um estudo explorativo e descritivo utilizando o Sistema de Classificação de Pacientes e a Resolução COFEN 293/2004. A taxa de ocupação foi de 92,6 por cento. Dos pacientes atendidos 46 por cento são cuidados intensivos, 30,9 por cento semi-intensivos, 21,7 por cento intermediários e 1,29 por cento cuidados mínimos. A equipe de três enfermeiros e 19 técnicos técnicos de enfermagem deve ser adequada para 10 e 12 profissionais respectivamente. O dimensionamento é a etapa inicial para uma assistência ideal.
