Signal and reward in wild fleshy fruits: Does fruit scent predict nutrient content?

Plant species with fleshy fruits offer animals rewards such as sugar, protein, and fat, to feed on their fruits and disperse their seeds. They have also evolved visual and olfactory signals indicating their presence and ripeness.In some systems, fruit color serves as a reliable visual signal of nutrient content. Yet even though many volatile chemicals used as olfactory signals derive from nutrients animals seek, it is still unknown whether fruit scent encodes information regarding nutrient content in wild fruits.We examine the relationship between olfactory signals and nutrient rewards in 28 fruiting plant species in Madagascar. We measured the relative amounts of four chemical classes in fruit scent using gas chromatography and mass spectrometry, as well as the relative amounts of sugar and protein in fruit pulp.We found that protein levels are not associated with elevated amounts of chemically related volatile compounds in fruit scent. In contrast, sugar content is strongly associated with the chemical composition of fruit scent.To our knowledge, this is the first research to explore the connection between fruit chemical signals and nutrient rewards. Our results imply that in the case of sugar, fruit scent is predictive of nutrient content and hence an honest signal.

Nighttime stomatal conductance differs with nutrient availability in two temperate floodplain tree species.

Nighttime water flow varies between plant species and is a phenomenon for which the magnitude, purpose and consequences are widely discussed. A potential benefit of nighttime stomata opening may be increased nutrient availability during the night since transpiration affects the mass flow of soil water towards plant roots. We investigated how nitrogen (N) and phosphorus (P) fertilization, and short-term drought affected stomatal conductance of Fraxinus excelsior L. and Ulmus laevis Pallas during the day (gs) and night (gn), and how these factors affected growth for a period of 18 weeks. Both species were found to open their stomata during the night, and gn responded to nutrients and water in a different manner than gs. Under N-deficiency, F. excelsior had higher gn, especially when P was sufficient, and lower pre-dawn leaf water potential (Ψpd), supporting our assumption that nutrient limitation leads to increases in nighttime water uptake. Under P-deficiency, F. excelsior had higher relative root production and, thus, adjusted its biomass allocation under P shortage, while sufficient N but not P contributed to overall higher biomasses. In contrast, U. laevis had higher gn and lower root:shoot ratio under high nutrient (especially N) availability, whereas both sufficient N and P produced higher biomasses. Compared with well-watered trees, the drought treatment did not affect any growth parameter but it resulted in lower gn, minimum stomatal conductance and Ψpd of F. excelsior. For U. laevis, only gs during July was lower when drought-treated. In summary, the responses of gs and gn to nutrients and drought depended on the species and its nutrient uptake strategy, and also the timing of measurement during the growing season. Eutrophication of floodplain forests dominated by F. excelsior and U. laevis may, therefore, considerably change nighttime transpiration rates, leading to ecosystem-level changes in plant-water dynamics. Such changes may have more severe consequences in the future as a higher frequency of drought events is predicted under climate change.

The importance of protein in leaf selection of folivorous primates.

Protein limitation has been considered a key factor in hypotheses on the evolution of life history and animal communities, suggesting that animals should prioritize protein in their food choice. This contrasts with the limited support that food selection studies have provided for such a priority in nonhuman primates, particularly for folivores. Here, we suggest that this discrepancy can be resolved if folivores only need to select for high protein leaves when average protein concentration in the habitat is low. To test the prediction, we applied meta-analyses to analyze published and unpublished results of food selection for protein and fiber concentrations from 24 studies (some with multiple species) of folivorous primates. To counter potential methodological flaws, we differentiated between methods analyzing total nitrogen and soluble protein concentrations. We used a meta-analysis to test for the effect of protein on food selection by primates and found a significant effect of soluble protein concentrations, but a non-significant effect for total nitrogen. Furthermore, selection for soluble protein was reinforced in forests where protein was less available. Selection for low fiber content was significant but unrelated to the fiber concentrations in representative leaf samples of a given forest. There was no relationship (either negative or positive) between the concentration of protein and fiber in the food or in representative samples of leaves. Overall our study suggests that protein selection is influenced by the protein availability in the environment, explaining the sometimes contradictory results in previous studies on protein selection. Am. J. Primatol. 79:e22550, 2017. © 2016 Wiley Periodicals, Inc.