RESUMO
The extent of drought tolerance in the seedlings of three wheat cultivars (WMRI-1, BARI GOM-33 and BARI GOM-21) was investigated by seed and root priming using abscisic acid (ABA) and glycine betaine (GB). The seeds were primed with ABA (10 and 20 µM) and GB (50 and 100 mM) and grown in pots maintaining control (0 % PEG) and drought (10 % PEG) conditions. Under drought, the root and shoot length, root and shoot biomass were significantly increased in ABA and GB primed seedlings than non-primed seedlings in all cultivars. Among the priming agents, either 20 µM ABA or 50 mM GB triggered better seedling growth in all wheat cultivars. These two levels were then applied with the nutrient solution in the hydroponics following four treatments: Control, Drought, Drought + ABA and Drought + GB. The seedling growth significantly declined in drought, while an improved seedling growth was observed in ABA and GB-treated plants in all cultivars. A considerable increase in lipid peroxidation, proline content, total antioxidant capacity and total flavonoid content in roots and leaves were recorded in all drought conditions, while these values were considerably reduced in ABA and GB treatments. Hierarchical clustering heatmap using stress tolerance index (STI) values showed that Drought + ABA and Drought + GB secured higher STI scores suggesting a greater degree of drought tolerance in all cultivars. In conclusion, seed and root priming of ABA and GB enhanced drought tolerance in the wheat seedlings by improving seedling growth and antioxidative defense suggesting a declined state of oxidative damage.
RESUMO
Heavy metal enrichment in road dust has resulted from intensive anthropogenic activity, particularly urbanization, industrial activities and traffic emission, posing a hazard to urban ecosystems and human health. To promote optimal road dust management in urban environments, it is necessary to assess the possible ecological and health impact of toxic elements in road dust. In a heavily populated megacity like Dhaka, Bangladesh, large-scale risk assessments of contamination in road dust with heavy metals are limited. The present study aims at presenting a concentration of twenty-five metals in road dust (Na, K, Cs, Rb, Mg, Ca, Sr, Ba, Al, Zn, Cd, Pb, As, Sb, Sc, Ti, V, Cr, Mn, Fe, Co, Ni, Cu, Zr and W) in Dhaka megacity. We used a critical source-based positive matrix factorization model, source-oriented potential ecological risks and health risks. Out of the studied metals, Na, Ca, Zn, Cd, Cu, Zr and W exceeded the shale value. About 73%, 48%, 29% and 32% of sampling sites showed a higher level of pollution based on PLI, NIPI, PER and NIRI, respectively. PMF model identified that Cd (85.3%), Cr (62.4%), Ni (58.2%), Zn (81.8%) and Mn (65.9%) in road dust were primarily attributed to traffic emission, fuel combustion, metal processing, transport sources and natural sources, respectively. Fuel combustion and metal processing posed considerable and high risks based on modified potential ecological risk and NIRI. Based on health hazards, traffic emission posed a high cancer risk in adult males (29%), whereas transport sources contributed to females (21%) and children (23%).