Malnutrition and environmental stimulation in rats: wave latencies of the brainstem auditory evoked potentials.

It has been shown that environmental stimulation may reduce the damage caused by malnutrition to morphological and behavioural parameters; however, there are no data on the effects of stimulation on the Brainstem Auditory Evoked Potentials (BAEPs). The aim of this study was to evaluate the effects of protein malnutrition, nutritional recovery and environmental stimulation on the BAEPs of the rat. On the first day of life, the animals were divided into Well-nourished (W) and Malnourished (M) groups. At weaning, half the M rats were submitted to nutritional recovery (R) until the test day. All groups were subdivided into Stimulated (S) and Non-Stimulated (N) rats. BAEPs was tested in animals exposed to clicks of 90, 80 and 70 dB of intensity. The BAEPs latencies of waves I, II, III and IV in the left ear were analysed in independent groups of rats on the 14th, 18th, 22nd, 32nd, and 42nd days of age. Statistical analysis showed diet and environmental stimulation interaction on the latencies of waves I, II, III and IV at all tested ages. WN rats showed longer latencies of waves I, II, III and IV than WS rats, and MN rats also showed longer latencies of these waves compared to WN, MS and RN at all tested ages. The results showed that malnutrition caused a delay in the latency of all BAEPs waves in rats of all ages. However, environmental stimulation reduced these latencies, reversing some damage caused by malnutrition. These data suggest that the auditory brainstem pathway is vulnerable to nutritional insults, and its structures show plasticity with environmental stimulation.

Response threshold to aversive stimuli in stimulated early protein-malnourished rats.

Two animal models of pain were used to study the effects of short-term protein malnutrition and environmental stimulation on the response threshold to aversive stimuli. Eighty male Wistar rats were used. Half of the pups were submitted to malnutrition by feeding their mothers a 6% protein diet from 0 to 21 days of age while the mothers of the other half (controls) were well nourished, receiving 16% protein. From 22 to 70 days all rats were fed commercial lab chow. Half of the animals in the malnourished and control groups were maintained under stimulating conditions, including a 3-min daily handling from 0 to 70 days and an enriched living cage after weaning. The other half was reared in a standard living cage. At 70 days, independent groups of rats were exposed to the shock threshold or to the tail-flick test. The results showed lower body and brain weights in malnourished rats when compared with controls at weaning and testing. In the shock threshold test the malnourished animals were more sensitive to electric shock and environmental stimulation increased the shock threshold. No differences due to diet or environmental stimulation were found in the tail-flick procedure. These results demonstrate that protein malnutrition imposed only during the lactation period is efficient in inducing hyperreactivity to electric shock and that environmental stimulation attenuates the differences in shock threshold produced by protein malnutrition.

Response threshold to aversive stimulated early protein-malnourished rats

Two animal models of pain were used to study the effects of short-term protein malnutrition and environmental stimulation on the response threshold to aversive stimuli. Eighty male Wistar rats were used. Half of the pups were submitted to malnutrition by feeding their mothers a 6 per cent protein diet from 0 to 21 days of age while the mothers of the other half (controls) were well nourished, receiving 16 per cent protein. From 22 to 70 days all rats were fed commercial lab chow. Half of the animals in the malnourished and control groups were maintained under stimulating conditions, including a 3-min daily handling from 0 to 70 days and an enriched living cage after weaning. The other half was reared in a standard living cage. At 70 days, independent groups of rats were exposed to the shock threshold or to the tail-flick test. The results showed lower body and brain weights in malnourished rats when compared with controls at weaning and testing. In the shock threshold test the malnourished animals were more sensitive to electric shock and environmental stimulation increased the shock threshold. No differences due to diet or environmental stimulation were found in the tail-flick procedure. These results demonstrate that protein malnutrition imposed only during the lactation period is efficient in inducing hyperreactivity to electric, shock and that environmental stimulation attenuates the differences in shock threshold produced by protein malnutrition.