Anti-wrinkle and anti-whitening effects of jucá (Libidibia ferrea Mart.) extracts.

Skin aging is a natural process of the human body that may be accelerated due to extrinsic causes. Libidibia ferrea, popularly known as jucá, is a small tree, which possesses an abundant phenolic composition with potential antioxidant and enzymatic inhibition activities. Thus, this work aimed to investigate the anti-wrinkle and anti-whitening potentials of jucá trunk bark (LFB) and pod (LFP) extracts. A comprehensive analysis of LFB and LFP phenolic composition was accomplished by means of liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). Effects on skin degradation were assessed by inhibitory enzymatic activity against elastase, hyaluronidase and collagenase through colorimetric assays. Cellular viability in B16F10 and primary fibroblasts were determined by Trypan Blue exclusion assay. Anti-melanogenic effects on B16F10 cells were evaluated using cellular tyrosinase, melanin content, western blot, and RT-qPCR analyses. Inhibition of matrix metalloproteinase-2 and metalloproteinase-9 (MMP-2 and MMP-9) was determined by gelatin zymography and western blot methodologies. LC-MS/MS analyses of LFB and LFP extracts allowed the characterization of 18 compounds, among them, flavonoids, phenolic acids, and secoridoids. Additionally the pod and trunk bark compositions were compared. Hyaluronidase inhibitory activity for both extracts, LFB (IC50 = 8.5 ± 0.8 µg/mL) and LFP (IC50 = 16 ± 0.5 µg/mL), was stronger than standard rutin (IC50 = 27.6 ± 0.06). Pro-MMP-2 was significantly inhibited by both extracts. LFB and LFP decreased the melanin content in B16F10 due to tyrosinase inhibitory activity. L. ferrea extracts has high potential as a cosmetic ingredient due to its anti-wrinkle and depigmentant effects.

Herbal mouthwash based on Libidibia ferrea: microbiological control, sensory characteristics, sedimentation, pH and density / Enxaguatório bucal fitoterápico à base de Libidibia ferrea: controle microbiológico, características organolépticas, sedimentação, pH e densidade

Introduction: Phytotherapy is the study of herbal medicines and their applicability to cure diseases in general, being a therapeutic method which can be used for the prevention and treatment of mouth diseases. Among the herbal studied, the Libidibia ferrea, known as jucá or ironwood, is widely used in folk medicine by presenting anti-inflammatory, analgesic, antimicrobial and antipyretic therapeutic properties. Objective: To evaluate in vitro pharmacological stability of the Libidibia ferrea extract's mouthwash (INPA - 228 022). Material and method: It was held the mouthwash microbiological control by determining the total number of microorganisms and Salmonella sp, Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus; stability characteristics (color, odor, brightness and consistency), sedimentation test (centrifuge), the pH measurement (pH meter) and density evaluation (pycnometer) were analyzed. Result: The mouthwash showed to be absent from microorganisms and no changes were observed in the organoleptics and sedimentation characteristics. The average pH values were 6.21, 6.15 and 5.85 at 0, 30 and 60 days, respectively, and 1.029, 1.033 and 1.035 g/ mL density values, respectively, without interfering with the final characteristic of the formulation. Conclusion: The mouthwash presented pharmacological stability and quality conditions. .

Introdução: A Fitoterapia é o estudo de plantas medicinais e suas aplicabilidades para a cura de doenças em geral, constituindo um método terapêutico que pode ser utilizado para a prevenção e tratamento de doenças bucais. Dentre as plantas estudadas, a Libidibia ferrea, conhecida como jucá ou pau ferro, é bastante utilizada na medicina popular por apresentar propriedades terapêuticas anti-inflamatória, analgésica, antimicrobiana e antitérmica. Objetivo: Avaliar, in vitro, a estabilidade farmacológica de um enxaguatório bucal fitoterápico à base do extrato de Libidibia ferrea (228.022 - INPA). Material e método: Foi realizado o controle microbiológico do enxaguatório através da determinação do número total de microrganismos de Salmonella sp., Escherichia coli, Pseudomonas aeruginosa e Staphylococcus aureus; foram analisadas as características organolépticas (cor, odor, brilho e consistência), sedimentação (centrífuga), aferição do pH (peagâmetro) e densidade (picnômetro). Resultado: O enxaguatório mostrou-se ausente de microrganismos e não foram observadas alterações das características organolépticas e sedimentação. Os valores médios de pH foram de 6,21, 6,15 e 5,85 nos tempos de armazenamento de 0, 30 e 60 dias, respectivamente, e de densidade 1,029, 1,033 e 1,035 g/ mL, respectivamente, porém sem interferência na característica final da formulação. Conclusão: O enxaguatório à base de Libidibia ferrea apresentou condições de estabilidade e qualidade farmacológicas. .

Evaluation of the stability and antimicrobial activity of an ethanolic extract of Libidibia ferrea.

Biofilm is a dense, whitish, noncalcified aggregate of bacteria, with desquamated epithelial cells and food debris creating conditions for an imbalance of resident oral microflora and favoring the destruction of hard and soft tissues by development of caries and gingivitis. The aim of this study was to obtain and characterize an extract of Libidibia ferrea, ex Caesalpinia ferrea L. and to evaluate its feasibility for formulation as a mouthwash, according to current legislation. For this purpose, pH, sedimentation, density, and stability were evaluated, along with microbiological testing of the extract. The microbiological test was used to verify the presence of Staphylococcus aureus, Pseudomonas aeruginosa, fungi, yeasts, coliforms, and minimum inhibitory concentrations of Streptococcus mutans and Streptococcus oralis strains. Characterization, microbiological evaluation, and minimum inhibitory concentration results were tabulated and described using descriptive statistics. The L. ferrea extract showed stable characteristics, product quality, and antibacterial activity against the microorganisms tested irrespective of experimental time intervals. According to these results, it can be concluded that formulation of a mouthwash containing L. ferrea extract to control biofilm is feasible, but further studies are needed.