An efficient, fast, and green procedure to quantify α- and ß-acids and xanthohumol in hops and their derived products.

The main precursors of bitter principles of beer are α-acids, followed by ß-acids. Determination of their content in hop cones and their derivatives is an essential part of brewing. HPLC-UV is the preferred technique when a more precise quantification of some specific bitter acids are required. However, current methods do not allow the proper quantification of all the six major α- and ß-acids; furthermore, these multi-step methods generate considerable volumes of chemical waste. In this work, a new UHPLC-UV protocol was developed and compared side-by-side with reference, considering traditional and sustainability parameters. Baseline separations were achieved for the six major bitter acids and xanthohumol, while a greener and faster sample preparation procedure was developed. The new validated procedure could be adopted for quality control of hops and their derivative products with enhanced sample throughput and with a potential gain in the safety of the analyst.

Thermal stress related with mortality rates on broilers' preslaughter operations: a lairage time effect study / Estresse térmico relacionado com taxas de mortalidade durante as operações pré-abate de frangos de corte: um estudo sobre os efeitos do tempo de espera

Concerning the reduction of preslaughter death losses, high temperature and relative humidity in the tropics are the major concerns regarding broiler survival prior slaughtering. However, the relationship between different lairage times under controlled environment and the thermal condition outside the holding area is still unclear. Thus, the aim of this study was to compare different intervals of lairage time with different environmental temperature and relationship with poultry mortality rates. A study was conducted in a commercial poultry abattoir in the State of Sao Paulo, Brazil, in the year of 2006. The historical data set from 13,937 trucks, about broiler mortality during preslaughter operations, was given by the abattoir. Factors that influence the welfare of birds were studied, such as lairage time in abattoir and hourly dry-bulb temperature. The statistical analysis was performed using the Double Generalized Linear Models. The lower incidence of death losses before arrival at the processing plant (approximately 13 dead birds per truck) was observed when the lairage time was between 1 to 3 hours, under high temperatures (above 22°C). This effect was more pronounced under critical (25-28°C) and lethal intervals (above 29°C) (12 and 13 dead birds per truck, respectively). In relation to lairage time, the reduction in mortality rates was more pronounced in the intervals up to 1 hour of lairage under climatized conditions, with a reduction of 3 dead birds per truck. It was concluded that thermal stress have negative influence for broilers welfare kept under preslaughter conditions and variations on mortality rate are strongly related with different lairage times.

No que diz respeito à redução das perdas por mortalidade pré-abate, as temperaturas e umidades relativas elevadas nos trópicos são as maiores preocupações quanto à sobrevivência de frangos de corte antes do abate. Entretanto, a relação entre diferentes tempos de espera em ambiente controlado e a condição térmica fora do galpão de espera ainda não está clara. Dessa forma, objetivou-se por meio deste trabalho comparar diferentes tempos de espera com diferentes temperaturas externas e a influência nas taxas de mortalidade de frangos de corte. O estudo foi conduzido em um abatedouro comercial de frangos de corte, situado no Estado de São Paulo, Brasil, durante o ano de 2006. Dados históricos de mortalidade de aves durante as operações pré-abate foram obtidos no abatedouro, provenientes de 13.937 caminhões transportadores de frangos de corte. Fatores que influenciam o bem estar das aves foram estudados, dentre eles, o tempo de espera no abatedouro e a temperatura horária do ambiente externo. A análise estatística foi realizada por meio dos Modelos Lineares Generalizados Duplos. A baixa incidência de mortes antes da chegada à linha de abate foi observada (aproximadamente 13 aves mortas por caminhão) quando o tempo de espera esteve entre 1 e 3 horas, sob altas temperaturas (acima de 22°C). Esse efeito foi mais evidente nas faixas crítica (25-28°C) e letal (acima de 29°C) (12 e 13 aves mortas por caminhão, respectivamente). Com relação ao tempo de espera, a redução da mortalidade foi mais pronunciada em intervalos acima de uma hora de espera, sob condições de espera climatizada. Conclui-se que o estresse térmico possui influência negativa para o bem-estar de frangos mantidos sob condição pré-abate e variações na taxa de mortalidade são fortemente relacionadas aos diferentes tempos de espera.