RESUMO
CONTEXT: Cyclolepis genistoides D. Don (Asteraceae) is an Argentinean endemic shrub, known by the vernacular name "palo azul" or "matorro negro". It is widely used in folk medicine as a diuretic, an antirheumatic, and an antispasmodic agent. OBJECTIVE: The aim of this work was to describe the activity of two isolated compounds of C. genistoides, oleanolic acid (1) and deacylcynaropicrin (2), using the carrageenan-induced paw edema method in mice. MATERIALS AND METHODS: Aerial parts of C. genistoides were dried, powdered, and extracted with petroleum ether, ethyl acetate, dichloromethane, and methanol for 48 h. The fractions obtained from the ethyl acetate extract yielded oleanolic acid, while deacylcynaropicrin was obtained according to Abdei-Mogib et al. Structures were elucidated by ¹H-NMR and ¹³C-NMR. The products were administered intraperitoneally at doses of 40, 75, and 100 mg/kg. RESULTS AND DISCUSSION: Compound 1 exhibited significant activity during the first 7 h of the inflammatory phase (at 1, 3, 5, and 7 h), exercising its inhibitory action on inflammation mediated by histamine, prostaglandins, serotonin, and kinins, while compound 2 showed a significant inhibition at 3 and 5 h contributing to this effect, acting in the intermediate phase. CONCLUSIONS: According to the results of this work, the intraperitoneal administration of oleanolic acid and deacylcynaropicrin isolated from the aerial parts of C. genistoides produced a significant inhibition of carrageenan-induced inflammation at doses of 75 and 100 mg/kg. These results give support to the use of this plant as an anti-inflammatory in traditional medicine.
Assuntos
Anti-Inflamatórios/farmacologia , Asteraceae/química , Lactonas/farmacologia , Ácido Oleanólico/farmacologia , Sesquiterpenos/farmacologia , Animais , Anti-Inflamatórios/administração & dosagem , Anti-Inflamatórios/isolamento & purificação , Carragenina , Modelos Animais de Doenças , Relação Dose-Resposta a Droga , Feminino , Inflamação/tratamento farmacológico , Inflamação/fisiopatologia , Injeções Intraperitoneais , Lactonas/administração & dosagem , Lactonas/isolamento & purificação , Masculino , Medicina Tradicional , Camundongos , Ácido Oleanólico/administração & dosagem , Ácido Oleanólico/isolamento & purificação , Componentes Aéreos da Planta , Extratos Vegetais/administração & dosagem , Extratos Vegetais/farmacologia , Sesquiterpenos/administração & dosagem , Sesquiterpenos/isolamento & purificação , Fatores de TempoRESUMO
In addition to the known mulinolic and mulinenic acids, two diterpenoids, mulin-11-ene-13-alpha,14-alpha-dihydroxy-20-oic and mulin-12-ene-14-one-20-oic acids, were isolated from the aerial parts of Mulinum spinosum (Cav.) Pers. (Apiaceae). Their structures were determined based on spectroscopic studies.
Assuntos
Apiaceae/química , Diterpenos/química , Diterpenos/isolamento & purificação , Espectroscopia de Ressonância Magnética , Estrutura Molecular , Componentes Aéreos da Planta/química , Plantas Medicinais/químicaRESUMO
Three diterpenoid derivatives were isolated from the acetone extract of Baccharis flabellata. Their structures were elucidated as 2,19;15,16-diepoxy-neo-clerodan-3,13(16),14-trien-18-oic acid, 15,16-epoxy-5,10-seco-clerodan-1(10),2,4,13(16),14-pentaen-18,19-olide and 15,16-epoxy-neo-clerodan-1,3,13(16),14-tetraen-18,19-olide through spectroscopic analyses.