RESUMO
Latent infection with adenovirus and respiratory syncytial virus (RSV) is associated with chronic obstructive pulmonary disease (COPD). The role of respiratory viral infections are emerging in COPD exacerbations. The present study aimed to investigate the prevalence of adenovirus and RSV serotypes A and B in individuals with acute exacerbations of COPD (COPD-AE) and stable COPD. Twenty seven patients with COPD-AE were evaluated using a prospective longitudinal study design. Induced sputum, sera and nasal smears were sampled from patients experiencing COPD-AE and those in a stable condition. Adenoplex® multiplex polymerase chain reaction (PCR) kits and Invitek RTP® DNA/RNA Virus Mini kits were used for PCR assays of adenovirus and RSV, respectively. Eighteen patients who experienced a COPD-AE were also evaluated while in a stable condition. The results showed that three sputum samples were positive for adenovirus in patients experiencing an exacerbation, while one was positive among the patients in a stable condition. RSV serotype A was detected in 17/27 (63%) patients with COPD-AE and 10/18 (55.6%) patients in a stable condition. RSV serotype B was not detected. Patients with COPD-AE, who were positive for RSV serotype A exhibited higher serum fibrinogen levels than those who were negative (438.60 ± 126.08 mg/dl compared with 287.60 ± 85.91 mg/dl; P=0.004). Eight/ten patients who were positive for RSV serotype A while in a stable condition, were also positive during COPD-AE. The results of the present study suggested that RSV infection may be prevalent in patients with COPD-AE and in those in a stable condition. Therefore, chronic RSV infection may occur in COPD. The detection and prevention of RSV may be useful in the management of COPD.
Assuntos
Infecções por Adenoviridae/diagnóstico , Adenoviridae/genética , Doença Pulmonar Obstrutiva Crônica/diagnóstico , Infecções por Vírus Respiratório Sincicial/diagnóstico , Vírus Sinciciais Respiratórios/genética , Adenoviridae/isolamento & purificação , Infecções por Adenoviridae/patologia , Infecções por Adenoviridae/virologia , Adulto , Idoso , Idoso de 80 Anos ou mais , Feminino , Humanos , Estudos Longitudinais , Masculino , Pessoa de Meia-Idade , Reação em Cadeia da Polimerase Multiplex , Estudos Prospectivos , Doença Pulmonar Obstrutiva Crônica/patologia , Doença Pulmonar Obstrutiva Crônica/virologia , Infecções por Vírus Respiratório Sincicial/patologia , Infecções por Vírus Respiratório Sincicial/virologia , Vírus Sinciciais Respiratórios/isolamento & purificação , Sorotipagem , Índice de Gravidade de Doença , Escarro/virologiaRESUMO
OBJECTIVE: Cytomegalovirus (CMV) is a significant cause of morbidity and mortality in allogeneic hematopoietic stem cell transplantation (AHSCT) recipients. Current practice includes prophylactic and preemptive treatment modalities, which have risks, side effects, and costs of their own. There is no established risk scoring system that applies to all patients. We aimed to investigate the risk factors for CMV reactivation in AHSCT recipients. MATERIALS AND METHODS: We retrospectively analyzed the risk factors for CMV reactivation in 185 consequent AHSCT recipients transplanted between September 2003 and December 2009 at the Stem Cell Transplantation Unit of Gazi University. Besides the standard transplant-related parameters, HLA antigens were also included among the variables analyzed. RESULTS: Despite the very high rate of donor (94.6%) and recipient (100%) seropositivity, which are the so-called major risk factors in previous reports, our reactivation rate was much lower, with a frequency of 24.9%. The underlying disease, sex, conditioning regimen, and presence of antithymocyte globulin or fludarabine in the conditioning regimen had no impact on reactivation rate. CMV reactivation was significantly more frequent in recipients with graft-versus-host disease (GVHD) compared to those without GVHD (p<0.0001). CMV reactivation was significantly more frequent (p<0.05) in patients with HLA-B14, HLA-DRB1*01, and HLA-DRB1*13 antigens and less frequent in recipients with HLA-A11 and HLA-DRB1*04 antigens (p<0.05). CONCLUSION: Universal risk factors/scores that apply to all transplant recipients are required for tailored prophylaxis and/or treatment strategies for CMV reactivation. Uncovering the role of genetic factors, including HLA antigens, as possible risk factors might lead the way to risk-adaptive strategies for adoptive cellular therapy and/or vaccination.
RESUMO
UNLABELLED: Epstein-Barr virus (EBV) is a well-known carcinogenic virus, and the association of EBV with some tumours suggests that there may also be an association between laryngeal carcinoma and EBV. OBJECTIVE: The aim of this study is to determine the role of EBV in the aetiology of laryngeal carcinoma. METHOD: Prospective investigation the EBV with real time polymerase chain reaction in tumour tissues of 25 patients with laryngeal carcinoma and 17 patients with benign laryngeal lesions, and investigation of the relationship between the presence of viral DNA and patients' smoking habits, alcohol consumption, localization and differentiation of the tumour. RESULTS: There was no significant difference between the control group and patient group in terms of EBV polymerase chain reaction positivity (p > 0.05). Also we couldn't find a statistically significant relationship between EBV positivity and differentiation of the tumour, localization of the tumour, smoking and alcohol consumption habits (p > 0.05). CONCLUSION: Our results suggest that, although EBV is present in some of the squamous cell laryngeal carcinomas, its presence has no effect on the pathogenesis of laryngeal carcinomas.
Assuntos
Carcinoma de Células Escamosas/virologia , DNA Viral/análise , Infecções por Vírus Epstein-Barr/complicações , Herpesvirus Humano 4/genética , Neoplasias Laríngeas/virologia , Adulto , Idoso , Consumo de Bebidas Alcoólicas/efeitos adversos , Carcinoma de Células Escamosas/patologia , Estudos de Casos e Controles , Herpesvirus Humano 4/isolamento & purificação , Humanos , Neoplasias Laríngeas/patologia , Masculino , Pessoa de Meia-Idade , Estudos Prospectivos , Reação em Cadeia da Polimerase em Tempo Real , Fatores de Risco , Fumar/efeitos adversosRESUMO
O vírus Epstein-Barr (EBV) é um conhecido vírus carcinogênico. A associação entre EBV e alguns tumores sugere que também pode haver correlação entre carcinoma de laringe e EBV. OBJETIVO: O presente estudo pretende determinar o papel do EBV na etiologia do carcinoma de laringe. MÉTODO: Estudo prospectivo sobre EBV por reação em cadeia da polimerase em tempo real em tecidos tumorais de 25 pacientes com carcinoma de laringe e 17 pacientes com lesões benignas de laringe; análise da relação entre presença de DNA viral e tabagismo, etilismo, localização e diferenciação tumoral. RESULTADOS: Não houve diferenças significativas entre os grupos de controle e de estudo para positividade da PCR para EBV (p > 0,05). Não foi identificada relação estatisticamente significativa entre positividade para EBV e diferenciação tumoral, localização da neoplasia, tabagismo ou etilismo (p > 0,05). CONCLUSÃO: Nossos resultados sugerem que, a despeito de sua identificação em alguns carcinomas espinocelulares de laringe, a presença de EBV não teve qualquer influência na patogenia do carcinoma de laringe.
Epstein-Barr virus (EBV) is a well-known carcinogenic virus, and the association of EBV with some tumours suggests that there may also be an association between laryngeal carcinoma and EBV. OBJECTIVE: The aim of this study is to determine the role of EBV in the aetiology of laryngeal carcinoma. METHOD: Prospective investigation the EBV with real time polymerase chain reaction in tumour tissues of 25 patients with laryngeal carcinoma and 17 patients with benign laryngeal lesions, and investigation of the relationship between the presence of viral DNA and patients' smoking habits, alcohol consumption, localization and differentiation of the tumour. RESULTS: There was no significant difference between the control group and patient group in terms of EBV polymerase chain reaction positivity (p > 0.05). Also we couldn't find a statistically significant relationship between EBV positivity and differentiation of the tumour, localization of the tumour, smoking and alcohol consumption habits (p > 0.05). CONCLUSION: Our results suggest that, although EBV is present in some of the squamous cell laryngeal carcinomas, its presence has no effect on the pathogenesis of laryngeal carcinomas.
Assuntos
Adulto , Idoso , Humanos , Masculino , Pessoa de Meia-Idade , Carcinoma de Células Escamosas/virologia , DNA Viral/análise , Infecções por Vírus Epstein-Barr/complicações , /genética , Neoplasias Laríngeas/virologia , Consumo de Bebidas Alcoólicas/efeitos adversos , Estudos de Casos e Controles , Carcinoma de Células Escamosas/patologia , /isolamento & purificação , Neoplasias Laríngeas/patologia , Estudos Prospectivos , Reação em Cadeia da Polimerase em Tempo Real , Fatores de Risco , Fumar/efeitos adversosRESUMO
To investigate the frequency of hepatitis B virus (HBV)-related events after allogeneic HCT in a moderate endemic area for HBV infection. The data of 197 patients who underwent allogeneic hematopoetic stem cell transplatation (HCT) from September 2003 through December 2010 were reviewed retrospectively with respect to HBV-related events. Resolved HBV infection was described as negative HBsAg, positive HBcAb, and positive HBsAb. Latent HBV infection was defined in patients with HBcAb positivity in the abscence of HBV DNA and HBsAb. Hepatitis B naive patients are defined as the patiens with no serological or molecular marker related to HBV. Seropositive patients were the patients with positive HBsAg and HBV-DNA. Median age was 28 (range, 15-64) years, with 128 male and 69 female patients. Median follow-up of the cohort was 8 (range, 0.5-78) months. We detected HBV-related events in 7 (3.6 %) recipients after allogeneic HCT. Five (71.4 %) of these events were HBV reactivation, while two cases (28.6 %) had acute hepatitis B infection. Four of the five reactivations were in the seropositive group (80 %), while one ocurred in a patient with resolved hepatitis. Two patients who developed acute hepatitis B were HBV naive and previously immunized patients, respectively. Hepatitis B virus reactivation remains a problem in seropositive patients and might require more effective treatment strategies.
Assuntos
Transplante de Células-Tronco Hematopoéticas/efeitos adversos , Vírus da Hepatite B/fisiologia , Hepatite B/sangue , Hepatite B/epidemiologia , Adolescente , Adulto , Estudos de Coortes , Feminino , Hepatite B/virologia , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Transplante Homólogo/efeitos adversos , Turquia/epidemiologia , Adulto JovemRESUMO
BACKGROUND: In Turkey, the Haemophilus influenzae type b-tetanus toxoid conjugate vaccine (Hib) was replaced by the combined diphtheria-tetanus-acellular pertussis and inactivated polio vaccine (DTaP-IPV/Hib) in 2008. This shift to the new schedule created different cohorts of vaccinated children as a consequence of the different schedules used. We evaluated the immunogenicity of the Hib vaccine in infants vaccinated with these different schedules. METHODS: Three groups of children were evaluated: group 1 comprised 145 infants vaccinated with diphtheria, tetanus, and whole cell pertussis (DTwP), oral polio vaccine (OPV), and Hib vaccines simultaneously at separate sites; group 2 comprised 204 infants vaccinated with the DTaP-IPV/Hib combined vaccine; group 3 comprised 100 infants vaccinated with a mixed schedule of DTwP, OPV, and Hib for the first one or two doses, followed by DTaP-IPV/Hib vaccine to complete the series. RESULTS: Anti-polyribosylribitol phosphate (anti-PRP) titers ≥0.15µg/ml were similar in groups 1, 2, and 3. However, in group 1, who received all the vaccines at separate sites, ≥ l.0µg/ml long-lasting antibody titers and anti-PRP geometric mean titers were higher (p=0.001). CONCLUSION: This study showed that even one dose administered in combination with other vaccines in a primary series decreased the level of anti-PRP.
Assuntos
Infecções por Haemophilus/prevenção & controle , Vacinas Anti-Haemophilus/administração & dosagem , Haemophilus influenzae tipo b/imunologia , Vacinação em Massa , Anticorpos Antibacterianos/sangue , Toxoide Diftérico/administração & dosagem , Vacinas Anti-Haemophilus/imunologia , Humanos , Lactente , Vacina contra Coqueluche/administração & dosagem , Vacina Antipólio de Vírus Inativado/administração & dosagem , Polissacarídeos/imunologia , Vigilância da População , Toxoide Tetânico/administração & dosagem , Turquia , Vacinas Acelulares/administração & dosagem , Vacinas Combinadas/administração & dosagem , Vacinas Combinadas/imunologiaRESUMO
Human polyomaviruses, namely BK (BKV) and JC (JCV) viruses are small DNA viruses that cause latent infections worldwide. Primary infections are usually acquired in the early periods of life and are generally asymptomatic. However BKV/JCV infections may cause severe clinical conditions in immunosuppressive patients such as bone marrow and solid organ transplantation or cancer patients. The aim of this retrospective study was to investigate the presence of BKV and JCV nucleic acids by real-time polymerase chain reaction (RT-PCR) in the clinical samples of patients with high risk. A total of 268 (62 blood, 206 urine) samples obtained from 115 immunocompromised patients hospitalized in Gazi University Hospital between July 2007 to January 2009, were included to the study. Viral nucleic acids were extracted from the samples with High Pure PCR Template Preparation Kit (Roche, Germany). By using amplification mix (TIB Molbiol GmbH, Germany) that included primers targeting 174 (JCV) and 219 (BKV) base pair fragments of the small t antigen, and hybridization probes (Roche, Germany), nucleic acids were amplified with LightCycler (Roche Applied Science, Germany) system. As a result, total polyomavirus DNA positivity rate was found as 33.2% (89/268). When BKV and JCV DNA positivities were evaluated according to the samples, 25.2% (53/206) of urine samples yielded positive results for BKV, 14.5% (30/206) for JCV and 2.4% (5/206) for both BKV and JCV. Only one of the blood samples (1/62; 1.6%) were found positive by means of BKV DNA, while none of the blood samples were positive for JCV DNA. The distribution of BKV and JCV DNA positivity rates according to the inpatient clinics were as follows, respectively; 24.3% and 9.5% for pediatric nephrology, 9.6% and 8.2% for renal transplantation unit, 13.5% and 18.9% for adult nephrology, 30.8% and 15.4% for bone marrow transplantation unit, 22.9% and 8.6% for pediatric clinics. In samples from pediatric hematology patients, BKV positivity was 36.4% (4/11), while there were no JCV positivity. However, in hematology patients, while JCV was positive in one of the three samples, no BKV positivity was detected.BKV was seen in three of six samples obtained from patients in the intensive care unit. JCV was positive in both of the two samples obtained from patients in pediatric endocrinology. The only patient that had BKV DNA in blood sample was a renal transplant patient. BKV + JCV DNAs were positive together in only five (1.9%) of the urine samples. In 24% (22/89) of the samples, BKV DNA was found ≥ 107 copies/ml, in 2.2% (2/89) JCV DNA was ≥ 107 copies/ml, whereas in 2.2% (2/89) of samples both BKV and JCV DNA was ≥ 107 copies/ml. All of those samples with high DNA levels were urine. The data of this study led to the establishment of a collaborative algorithm between the laboratory and clinics in our hospital for the diagnosis and follow-up of the patients in terms of BKV/JCV infections. In conclusion, since BKV/JCV reactivations and infections are crutial in immunosuppressive patients, especially medical centers specialized in bone marrow and renal transplantation, diagnostic and monitoring procedures related to those infections should be programmed.
Assuntos
Vírus BK/isolamento & purificação , Hospedeiro Imunocomprometido , Vírus JC/isolamento & purificação , Infecções por Polyomavirus/diagnóstico , Infecções Tumorais por Vírus/diagnóstico , Algoritmos , Vírus BK/genética , Transplante de Medula Óssea/efeitos adversos , Transplante de Medula Óssea/imunologia , DNA Viral/sangue , DNA Viral/isolamento & purificação , DNA Viral/urina , Humanos , Vírus JC/genética , Transplante de Rim/efeitos adversos , Transplante de Rim/imunologia , Neoplasias/complicações , Reação em Cadeia da Polimerase , Infecções por Polyomavirus/epidemiologia , Infecções por Polyomavirus/etiologia , Estudos Retrospectivos , Fatores de Risco , Infecções Tumorais por Vírus/epidemiologia , Infecções Tumorais por Vírus/etiologia , Turquia/epidemiologiaRESUMO
OBJECTIVE: Human cytomegalovirus (CMV) is the most common cause of viral intrauterine infections in the world. Herpes simplex virus type 2 (HSV-2) and human papillomavirus (HPV) are the main agents of viral sexually transmitted diseases, which cause genital ulcers and genital warts, respectively. HPV infection has been linked to the majority of the anogenital malignancies. The aim of this study was to detect the existence of CMV, HSV-2 and HPV type 16-18 in Turkish pregnants by using sensitive molecular assays. METHODS: One hundred thirty-four women (18-41 years old; mean age ± SD: 27 ± 8) applied to outpatient clinic of Obstetrics and Gynecology, in between 18th - 22nd weeks of their pregnancy and a control group of 99 healthy women (15-39 years old; mean age ± SD: 24 ± 8) were included in the study. Cervical smear samples were used for DNA extraction. CMV, HSV-2 and HPV 16-18 detections were carried out by real time PCR and in house PCR method, respectively. RESULTS: Three patients (3/134; 2.2 percent) were found to be positive for each HPV and HSV-2. Dual infection with HPV and HSV was found in just one patient. HPV 18 was detected in all positive samples. CMV was found to be positive in two patients (2/134; 1.4 percent). CONCLUSION: HPV, HSV and CMV must be screened due to high prevalence of these viruses in pregnants by using sensitive molecular methods.
Assuntos
Adolescente , Adulto , Feminino , Humanos , Masculino , Gravidez , Adulto Jovem , Infecções por Citomegalovirus/diagnóstico , Herpes Simples/diagnóstico , Infecções por Papillomavirus/diagnóstico , Complicações Infecciosas na Gravidez/diagnóstico , Algoritmos , Estudos de Casos e Controles , Seguimentos , /genética , /genética , /genética , Reação em Cadeia da Polimerase , Complicações Infecciosas na Gravidez/virologia , Sensibilidade e Especificidade , TurquiaRESUMO
AIM: To investigate the frequency of occult hepatitis B, the clinical course of hepatitis B virus (HBV) reactivation and reverse seroconversion and associated risk factors in autologous hematopoietic stem cell transplantation (HSCT) recipients. METHODS: This study was conducted in 90 patients undergoing autologous HSCT. Occult HBV infection was investigated by HBV-DNA analysis prior to transplantation, while HBV serology and liver function tests were screened prior to and serially after transplantation. HBV-related events including reverse seroconversion and reactivation were recorded in all patients. RESULTS: None of the patients had occult HBV prior to transplantation. Six (6.7%) patients were positive for HBV surface antigen (HBsAg) prior to transplantation and received lamivudine prophylaxis; they did not develop HBV reactivation after transplantation. Clinical HBV infection emerged in three patients after transplantation who had negative HBV-DNA prior to HSCT. Two of these three patients had HBV reactivation while one patient developed acute hepatitis B. Three patients had anti-HBc as the sole hepatitis B-related antibody prior to transplantation, two of whom developed hepatitis B reactivation while none of the patients with antibody to HBV surface antigen (anti-HBs) did so. The 14 anti-HBs- and/or anti-HBc-positive patients among the 90 HSCT recipients experienced either persistent (8 patients) or transient (6 patients) disappearance of anti-HBs and/or anti-HBc. HBsAg seroconversion and clinical hepatitis did not develop in these patients. Female gender and multiple myeloma emerged as risk factors for loss of antibody in regression analysis (P < 0.05). CONCLUSION: Anti-HBc as the sole HBV marker seems to be a risk factor for reactivation after autologous HSCT. Lamivudine prophylaxis in HbsAg-positive patients continues to be effective.
Assuntos
Transplante de Células-Tronco Hematopoéticas/efeitos adversos , Hepatite B/etiologia , Adolescente , Adulto , Idoso , Portador Sadio/virologia , DNA Viral/sangue , Feminino , Neoplasias Hematológicas/complicações , Neoplasias Hematológicas/terapia , Neoplasias Hematológicas/virologia , Hepatite B/imunologia , Hepatite B/virologia , Anticorpos Anti-Hepatite B/sangue , Humanos , Masculino , Pessoa de Meia-Idade , Mieloma Múltiplo/complicações , Mieloma Múltiplo/terapia , Mieloma Múltiplo/virologia , Fatores de Risco , Transplante Autólogo , Adulto JovemRESUMO
PURPOSE: this study was planned to evaluate the prevalence of HPV (excepting type 16) and HPV 16 by real-time PCR in colposcopy patients and to interprete the results with age, age of first sexual intercourse (FSI), parity and Pap smear results. METHODS: one hundred and two colposcopy patients (50 and 52 of the patients were classified as colposcopy positive and negative, respectively) applying to Gynecology clinic were included. HPV (excepting type 16) and HPV 16 were detected by realtime PCR using the L1 region. Real-time nested amplifications of MY09/11 products were done by GP5+/GP6+ primers and Cyanine-5 labeled HPV and HPV 16 DNA specific probe after HPV DNA extraction by phenol chloroform isoamylalcohol. RESULTS: HPV (excepting type 16) and HPV 16 were positive in 12% and 18% of the colposcopy positive patients respectively. HPV (excepting type 16) and HPV 16 were positive in 5.7% and 3.8% of the colposcopy negative patients, respectively. CONCLUSION: there was a statistically significant difference between colposcopy positive and colposcopy negative patients comparing HPV 16 with total HPV positivity (p = 0.021 for type 16 and p = 0.010 for total HPV) but there was not a statistically significant difference between colposcopy positive and colposcopy negative patients when we compared HPV (excepting type 16) positivity (p = 0.314). In conclusion, HPV detection and typing may be helpful for cervical cancer screening and prevention.
Assuntos
Alphapapillomavirus/genética , Colo do Útero/virologia , Infecções por Papillomavirus/diagnóstico , Adolescente , Adulto , Idoso , Alphapapillomavirus/isolamento & purificação , Colposcopia , DNA Viral/análise , Feminino , Genótipo , Papillomavirus Humano 16/genética , Papillomavirus Humano 16/isolamento & purificação , Humanos , Pessoa de Meia-Idade , Teste de Papanicolaou , Infecções por Papillomavirus/virologia , Reação em Cadeia da Polimerase/métodos , Prevalência , Sensibilidade e Especificidade , Esfregaço Vaginal , Adulto JovemRESUMO
PURPOSE: this study was planned to evaluate the prevalence of HPV (excepting type 16) and HPV 16 by real-time PCR in colposcopy patients and to interprete the results with age, age of first sexual intercourse (FSI), parity and Pap smear results. METHODS: one hundred and two colposcopy patients (50 and 52 of the patients were classified as colposcopy positive and negative, respectively) applying to Gynecology clinic were included. HPV (excepting type 16) and HPV 16 were detected by realtime PCR using the L1 region. Real-time nested amplifications of MY09/11 products were done by GP5+/GP6+ primers and Cyanine-5 labeled HPV and HPV 16 DNA specific probe after HPV DNA extraction by phenol chloroform isoamylalcohol. RESULTS: HPV (excepting type 16) and HPV 16 were positive in 12 percent and 18 percent of the colposcopy positive patients respectively. HPV (excepting type 16) and HPV 16 were positive in 5.7 percent and 3.8 percent of the colposcopy negative patients, respectively. CONCLUSION: there was a statistically significant difference between colposcopy positive and colposcopy negative patients comparing HPV 16 with total HPV positivity (p = 0.021 for type 16 and p = 0.010 for total HPV) but there was not a statistically significant difference between colposcopy positive and colposcopy negative patients when we compared HPV (excepting type 16) positivity (p = 0.314). In conclusion, HPV detection and typing may be helpful for cervical cancer screening and prevention.
Assuntos
Adolescente , Adulto , Idoso , Feminino , Humanos , Pessoa de Meia-Idade , Adulto Jovem , Alphapapillomavirus/genética , Colo do Útero/virologia , Infecções por Papillomavirus/diagnóstico , Alphapapillomavirus/isolamento & purificação , Colposcopia , DNA Viral/análise , Genótipo , /genética , /isolamento & purificação , Prevalência , Infecções por Papillomavirus/virologia , Reação em Cadeia da Polimerase/métodos , Sensibilidade e Especificidade , Esfregaço Vaginal , Adulto JovemRESUMO
OBJECTIVE: Human cytomegalovirus (CMV) is the most common cause of viral intrauterine infections in the world. Herpes simplex virus type 2 (HSV-2) and human papillomavirus (HPV) are the main agents of viral sexually transmitted diseases, which cause genital ulcers and genital warts, respectively. HPV infection has been linked to the majority of the anogenital malignancies. The aim of this study was to detect the existence of CMV, HSV-2 and HPV type 16-18 in Turkish pregnants by using sensitive molecular assays. METHODS: One hundred thirty-four women (18-41 years old; mean age ± SD: 27 ± 8) applied to outpatient clinic of Obstetrics and Gynecology, in between 18th - 22nd weeks of their pregnancy and a control group of 99 healthy women (15-39 years old; mean age ± SD: 24 ± 8) were included in the study. Cervical smear samples were used for DNA extraction. CMV, HSV-2 and HPV 16-18 detections were carried out by real time PCR and in house PCR method, respectively. RESULTS: Three patients (3/134; 2.2%) were found to be positive for each HPV and HSV-2. Dual infection with HPV and HSV was found in just one patient. HPV 18 was detected in all positive samples. CMV was found to be positive in two patients (2/134; 1.4 %). CONCLUSION: HPV, HSV and CMV must be screened due to high prevalence of these viruses in pregnants by using sensitive molecular methods.
Assuntos
Infecções por Citomegalovirus/diagnóstico , Herpes Simples/diagnóstico , Infecções por Papillomavirus/diagnóstico , Complicações Infecciosas na Gravidez/diagnóstico , Adolescente , Adulto , Algoritmos , Estudos de Casos e Controles , Feminino , Seguimentos , Herpesvirus Humano 2/genética , Papillomavirus Humano 16/genética , Papillomavirus Humano 18/genética , Humanos , Masculino , Reação em Cadeia da Polimerase , Gravidez , Complicações Infecciosas na Gravidez/virologia , Sensibilidade e Especificidade , Turquia , Adulto JovemRESUMO
EpsteinBarr virus (EBV) infection usually occurs in early childhood and can persist in palatine tonsil lymphocytes to induce tonsillitis at a later date. We have examined the presence of EBV in palatine tonsils and relationship between EBV-DNA quantity in tonsil tissues and VCA-IgG quantity in autologous sera. Tonsils were obtained from 36 patients, male 20 (55.6%), female 16 (44.4%) (mean age 7.96 ± 6.97 years), who underwent tonsils removal because of recurrent tonsillitis. Tissues were processed for real-time PCR and patient's sera were assayed to determine VCA-IgG by VCA-IgG ELISA. In 27 out of 36 cases (75%), positive EBV-DNA reaction was found. However, statistical analysis showed no correlation between EBV-DNA quantity and VCA-IgG quantity. We conclude that tonsils of children can be colonized by EBV and that virus may have a direct and indirect role in recurrent tonsillitis and nasopharyngeal carcinoma.
Assuntos
Antígenos Virais/análise , Proteínas do Capsídeo/análise , Infecções por Vírus Epstein-Barr/virologia , Herpesvirus Humano 4/isolamento & purificação , Imunoglobulina G/imunologia , Tonsila Palatina/virologia , Tonsilite/virologia , Carga Viral , Antígenos Virais/imunologia , Capsídeo/imunologia , Proteínas do Capsídeo/imunologia , Criança , DNA Viral/análise , Ensaio de Imunoadsorção Enzimática , Infecções por Vírus Epstein-Barr/sangue , Feminino , Seguimentos , Herpesvirus Humano 4/genética , Herpesvirus Humano 4/imunologia , Humanos , Masculino , Reação em Cadeia da Polimerase , Recidiva , Testes Sorológicos , Tonsilite/sangueRESUMO
Rotavirus was related to causing intussusception when the first generation rotavirus vaccine was introduced. However, the association between natural rotavirus infection and intussusception remains an area of concern and controversy. A few studies have found that rotavirus infection can cause intussusception. On the other hand, several studies were unable to find an association of intussusception with natural rotavirus infection. Herein, we describe a patient who developed intussusception following rotavirus diarrhea during the course of hospitalization and recovered by spontaneous resolution the next day. This rotavirus belonged to serotype G9P[6]. The case is presented here as an evidence that natural rotavirus infection is associated with intussusception. Comprehensive research is needed to identify whether intussusception by rotavirus has a propensity to resolve spontaneousey.
Assuntos
Intussuscepção/virologia , Infecções por Rotavirus/complicações , Rotavirus/classificação , Diarreia/virologia , Humanos , Lactente , Masculino , Remissão Espontânea , SorotipagemRESUMO
OBJECTIVE: In this study, we aimed to evaluate the role of human papillomavirus (HPV) in the clinical and histopathologic features of laryngeal and hypopharyngeal cancers. METHOD: Paraffin wax-embedded sections consisting of 89 laryngeal and hypopharyngeal cancers from 210 patients were analyzed. Tissue samples were amplified by using a glucose 6-phosphatase dehydrogenase (G6PDH) control kit (Eurogentec, Seraing, Belgium), and G6PDH-positive samples were thought to have appropriate tissues by using a deoxyribonucleic acid (DNA) extraction kit (DNA mini kit, Qiagen, Germany). HPV and HPV-16 were detected by real-time polymerase chain reaction (PCR) using the L1 region. Real-time nested amplifications of MY09/11 products were done by GP5+/GP6+ primers and a cyanine-5-labeled HPV-16 DNA-specific probe. HPV-16-negative MY09/11 amplicons were sequenced by an OpenGene automated DNA sequencing system, and a similar percentage of sequences was calculated by GeneObjects software (Visible Genetics, Canada). RESULTS: Specimens from 89 subjects fitting the criteria were taken for PCR assay, and the HPV genome was analyzed in 65 cases because the remaining cases did not have enough tissue according to G6PDH amplification. HPV was positive in 27 cases (41.5%). HPV positivity was found to be associated with lymph node metastasis (LNM). Odds ratio analysis indicated that HPV positivity was an important factor for LNM but not for other parameters. CONCLUSIONS: HPV-16 infection can be associated with laryngeal carcinomas without LNM. Analysis of HPV positivity could be used as a prognostic factor.
Assuntos
Glucosefosfato Desidrogenase/genética , Papillomavirus Humano 16/genética , Papillomavirus Humano 16/isolamento & purificação , Neoplasias Hipofaríngeas/virologia , Neoplasias Laríngeas/virologia , Papillomaviridae/isolamento & purificação , Infecções por Papillomavirus/genética , DNA/análise , Primers do DNA/genética , Feminino , Glote/patologia , Glote/virologia , Humanos , Neoplasias Hipofaríngeas/genética , Neoplasias Hipofaríngeas/patologia , Neoplasias Laríngeas/genética , Neoplasias Laríngeas/patologia , Masculino , Pessoa de Meia-Idade , Estadiamento de Neoplasias , Reação em Cadeia da Polimerase , Reação em Cadeia da Polimerase Via Transcriptase ReversaRESUMO
PURPOSE: Cervical carcinoma is the second most common cancer among women worldwide. Viral infections, especially human papillomavirus (HPV) infections, are important factors in its etiology. Changes in apoptotic regulation are considered to have an important role in the carcinogenesis development. In this study, the relationship between apoptosis and HPV infection was investigated. METHODS: HPV DNA and HPV DNA type 16 positivity were detected in 110 cervical smear samples with Real Time PCR and sequencing was performed for HPV DNA type 18. The presence of apoptosis was investigated using TUNEL and Annexin V staining methods and analyzed by fluorescence microscope and flow cytometry. RESULTS: HPV DNA type 16 was detected in 9 samples (8.1%), HPV DNA type 18 positive in 6 samples (5.4%) and HPV types other than HPV type 16 and HPV type 18 in 9 samples (8.1%). A decrease apoptosis was found in HPV DNA positive samples compared with controls (P < 0.05). CONCLUSION: The decrease of apoptosis during HPV infection might cause cellular immortality and then malignant transformation.
Assuntos
Infecções por Papillomavirus/patologia , Infecções por Papillomavirus/fisiopatologia , Cervicite Uterina/virologia , Anexina A5/análise , Apoptose , Colo do Útero/citologia , Colo do Útero/patologia , Colo do Útero/fisiologia , Colo do Útero/fisiopatologia , Feminino , Citometria de Fluxo , Papillomavirus Humano 16/isolamento & purificação , Humanos , Papillomaviridae , Neoplasias do Colo do Útero/patologia , Neoplasias do Colo do Útero/virologia , Cervicite Uterina/patologia , Cervicite Uterina/fisiopatologiaRESUMO
Between September 2004 and December 2005 a prospective study was conducted to understand the epidemiology of rotavirus infection among children with diarrhea attending two hospitals in Ankara, Turkey. Rotavirus was detected in 39.7% of the 322 stool samples and affected mainly children in the age group of 6-23 months. More than 70% and 39% of these cases occurred in children <2 and <1 year of age, respectively. In the temperate climate of Ankara rotavirus infection was prevalent throughout the year. Serotype G1P[8] was dominant followed by G9P[8]. In 38 samples a total of 5 electropherotypes were detected. All G9P[8] were of long electropherotype except one of short electropherotype. A proportion of G1 and G9 strains were in combination with P[6], P[4] or P nontypable. Mixed serotypes were responsible for 2.4% of the infections. A phylogenetic tree constructed with the deduced amino acid sequences of the VP7 gene showed that 16 Turkish G9 strains clustered with rotaviruses of lineage III. One G9 strain formed a new lineage, lineage IV with the Sri Lankan G9 rotaviruses. In the phylogenetic tree of the VP8* gene, the Turkish G9P[6] rotaviruses clustered with human strains of lineage Ia. Increased diversity of the G/P type combination and the presence of infection throughout the year in Turkey was a situation similar to developing countries. The occurrence of rotavirus infection at later age and low level of mixed infections in Turkey represented the situation of developed countries. This study suggests that diverse G9 rotaviruses are emerging in Turkey.
Assuntos
Diarreia/virologia , Infecções por Rotavirus/virologia , Rotavirus/classificação , Rotavirus/genética , Fatores Etários , Antígenos Virais/genética , Proteínas do Capsídeo/genética , Pré-Escolar , Análise por Conglomerados , Diarreia/epidemiologia , Fezes/virologia , Humanos , Lactente , Recém-Nascido , Epidemiologia Molecular , Dados de Sequência Molecular , Filogenia , Prevalência , Estudos Prospectivos , RNA Viral/genética , Proteínas de Ligação a RNA/genética , Rotavirus/isolamento & purificação , Infecções por Rotavirus/epidemiologia , Análise de Sequência de DNA , Homologia de Sequência de Aminoácidos , Sorotipagem , Turquia/epidemiologia , Proteínas não Estruturais Virais/genéticaRESUMO
It is assumed that various infectious agents play direct or indirect roles in the pathogenesis of atherosclerosis which is accepted as a chronic inflammatory phenomenon. However, the data obtained from different studies are contradictory. The aim of this study was to investigate the roles of herpes virus group [Herpes simplex virus (HSV), Epstein-Barr virus (EBV), Cytomegalovirus (CMV)] and hepatitis A virus (HAV) which are debated in terms of their impact in the pathogenesis of coronary arterial diseases. For this purpose, atherome plaque samples collected from 28 patients (23 were male; age range: 43-74 years) with atherosclerotic heart disease and vein samples from 22 control patients (19 were male; age range: 37-85 years) who had vascular diseases other than atherosclerosis, were investigated by means of the presence of nucleic acids of the above mentioned viruses by real-time polymerase chain reaction (PCR). Besides, classical cardiovascular risk factors (hypertension, hyperlipidemia, hypercholestrolemia, diabetes mellitus, smoking habits, gender, age and familial background) were questioned in both patient and control groups. As a result, no positivity were detected for nucleic acids of HSV type 1 and 2, EBV and HAV, whereas CMV-DNA was found positive in three of 28 (10.7%) atheromateous plaques (viral loads were 21, 188 and 288 copies/mg). Amongst 22 vascular samples from controls, two (9.1%) yielded positive results for EBV-DNA (viral loads were 5 and 10 copies/mg), while the other samples were found negative for nucleic acids of HSV type 1 and 2, CMV and HAV. The evaluation of the known risk factors for atherosclerosis revealed that, the difference between the presence of hypertension and hyperlipidemia which are the major risk factors, was statistically important (p < 0.05) in patient group (64% and 50%, respectively) and control group (32% and 23%, respectively). In conclusion, the hypothesis concerning the possible relationship between these viral agents and the progression of atherosclerosis, have not been supported by our data which are similar to the results obtained from various other studies. Actually, further studies are needed to clarify such direct or indirect roles of infectious agents in the pathogenesis of coronary arterial diseases.
Assuntos
Doença da Artéria Coronariana/virologia , Vírus da Hepatite A/isolamento & purificação , Herpesviridae/isolamento & purificação , Adulto , Idoso , Idoso de 80 Anos ou mais , Estudos de Casos e Controles , Doença da Artéria Coronariana/etiologia , DNA Viral/análise , Feminino , Vírus da Hepatite A/genética , Herpesviridae/genética , Humanos , Hipercolesterolemia/complicações , Hipertensão/complicações , Masculino , Pessoa de Meia-Idade , Reação em Cadeia da Polimerase , Fatores de RiscoRESUMO
OBJECTIVE: There is no specific antiviral therapy or a vaccine, which could be safely administered to the pregnant women with primary human cytomegalovirus (CMV) infection. Therefore, prenatal diagnosis has a critical role in the management of pregnancy, complicated by this disease. In this study, we investigated the prevalence and clinical consequences of human CMV infection from cervicovaginal smear and amniotic fluid samples of pregnant women by using real-time polymerase chain reaction (RT-PCR) assay, in one of the Obstetrics and Gynecology outpatient clinics of Turkey. The identification of reliable prognostic markers of fetal disease remains the main purpose and a major challenge on this issue. METHODS: Two hundred and six samples, of which 135 were cervicovaginal smear and 71 were amniotic fluid, were enrolled in the study. The DNAs of the samples were extracted by using Roche Diagnostic (Roche, Germany) kit and amplifications of these DNAs were studied by using Light-Cycler system (Roche Germany) as being quantitative. Anti-CMV IgM antibodies in the samples were studied by both MEIA (Imx system, Abbot Laboratories, USA) and a commercial ELISA kit (Radim SPA, Italy) while anti-CMV IgG antibodies were studied by MEIA (Axsym system, Abbot Laboratories, USA). RESULTS: Human CMV DNA was found to be positive in 1.5% (2 in 135) of cervicovaginal smear and 1.4% (1 in 71) of amniotic fluid samples by RT-PCR. IgM and IgG were found to be negative in all of the cervicovaginal smear samples by both MEIA and ELISA, while IgG antibody was found to be positive in only one of the amniotic fluid samples by MEIA. CONCLUSION: With RT-PCR assay, we have found the prevalence of human CMV in pregnant women similar to epidemiologic reports, which have been described earlier. Whereas the fetus with positive amniotic fluid in favor of human CMV had an intrauterine growth restriction resulted in intrauterine exitus, no symptoms were observed in the infants of the other two pregnant women with positive RT-PCR results. The fact that the clinical consequence of the newborn whose amniotic fluid evaluation revealed human CMV infection by RT-PCR made us think that this molecular diagnosis method may be a reliable assay in prenatal diagnosis of this pathogen.
Assuntos
Líquido Amniótico/virologia , Colo do Útero/virologia , Citomegalovirus/genética , Reação em Cadeia da Polimerase , Diagnóstico Pré-Natal/métodos , Vagina/virologia , Anticorpos Antivirais/análise , Afinidade de Anticorpos , Citomegalovirus/imunologia , Citomegalovirus/isolamento & purificação , Infecções por Citomegalovirus/diagnóstico , Infecções por Citomegalovirus/virologia , DNA Viral/análise , Ensaio de Imunoadsorção Enzimática , Feminino , Humanos , Imunoglobulina G/análise , Imunoglobulina M/análise , Gravidez , Prognóstico , Turquia , Esfregaço VaginalRESUMO
The clinical value of an in-house cytomegalovirus nested polymerase chain reaction (CMV-PCR) and a commercial molecular assay hybrid capture CMV DNA assay (HCA) was evaluated in monitoring a group of renal transplant patients for six months follow up. In this study, the sensitivity, specificity, positive predictive value, and negative predictive value of nested CMV DNA PCR assay and HCA at the beginning of the study were 70, 42.9, 46.7, 66.7, and 60, 78.6, 66.7, and 73.3% respectively. After six months, they were 80, 66.7, 80, 66.7 for CMV PCR and 73.3, 88.9, 91.7, 66.7% for HCA respectively. These results indicate that in monitoring and predicting CMV infections in renal transplant recipients, not only qualitative but also quantitative assays must be used together in order to decide the preemptive strategies.