RESUMO
Papillomaviruses (PVs) are highly species- and site-specific pathogens of stratified squamous epithelium. Although PV infections in the various Felidae are rarely reported, we identified productive infections in six cat species. PV-induced proliferative skin or mucous membrane lesions were confirmed by immunohistochemical screening for papillomavirus-specific capsid antigens. Seven monoclonal antibodies, each of which reacts with an immunodominant antigenic determinant of the bovine papillomavirus L1 gene product, revealed that feline PV capsid epitopes were conserved to various degrees. This battery of monoclonal antibodies established differential expression patterns among cutaneous and oral PVs of snow leopards and domestic cats, suggesting that they represent distinct viruses. Clinically, the lesions in all species and anatomic sites were locally extensive and frequently multiple. Histologically, the areas of epidermal hyperplasia were flat with a similarity to benign tumors induced by cutaneotropic, carcinogenic PVs in immunosuppressed human patients. Limited restriction endonuclease analyses of viral genomic DNA confirmed the variability among three viral genomes recovered from available frozen tissue. Because most previous PV isolates have been species specific, these studies suggest that at least eight different cat papillomaviruses infect the oral cavity (tentative designations: Asian lion, Panthera leo, P1PV; snow leopard, Panthera uncia, PuPV-1; bobcat, Felis rufus, FrPV; Florida panther, Felis concolor, FcPV; clouded leopard, Neofelis nebulosa, NnPV; and domestic cat, Felis domesticus, FdPV-2) or skin (domestic cat, F. domesticus, FdPV-1; and snow leopard, P. uncia, PuPV-2).
Assuntos
Carnívoros , Doenças do Gato/virologia , Papillomaviridae/classificação , Infecções por Papillomavirus/veterinária , Infecções Tumorais por Vírus/veterinária , Animais , Anticorpos Monoclonais , Southern Blotting/veterinária , Doenças do Gato/patologia , Gatos , DNA Viral/química , Mapeamento de Epitopos/veterinária , Feminino , Imuno-Histoquímica , Leões , Masculino , Microscopia Eletrônica , Papiloma/patologia , Papiloma/veterinária , Papiloma/virologia , Papillomaviridae/genética , Papillomaviridae/imunologia , Infecções por Papillomavirus/patologia , Infecções por Papillomavirus/virologia , Neoplasias Cutâneas/patologia , Neoplasias Cutâneas/veterinária , Neoplasias Cutâneas/virologia , Neoplasias da Língua/patologia , Neoplasias da Língua/veterinária , Neoplasias da Língua/virologia , Infecções Tumorais por Vírus/patologia , Infecções Tumorais por Vírus/virologiaRESUMO
Woodchucks were used to study the antiviral activity and toxicity of fialuridine (FIAU; 1,-2'deoxy-2'fluoro-1-beta-D-arabinofuranosyl-5-iodo-uracil). In an initial experiment, groups of six chronic woodchuck hepatitis virus (WHV) carrier woodchucks received daily doses of FIAU by intraperitoneal injection for 4 weeks. At 0.3 mg/kg/d, the antiviral effect was equivocal, but at 1.5 mg/kg/d, FIAU had significant antiviral activity. No evidence of drug toxicity was observed during the 4-week period of treatment or during posttreatment follow-up. In a second experiment, groups of nine WHV carriers or uninfected woodchucks were given 1.5 mg/kg/d of FIAU orally for 12 weeks, and the results compared with placebo-treated controls. After 4 weeks, the serum WHV-DNA concentration in the FIAU-treated carrier group was two to three logs lower than that in the placebo-treated group. After 12 weeks of FIAU treatment, serum WHV DNA was not detectable by conventional dot-blot analysis, hepatic WHV-DNA replicative intermediates (RI) had decreased 100-fold, and hepatic expression of WHV core antigen was remarkably decreased. No evidence of toxicity was observed after 4 weeks, but, after 6 to 7 weeks, food intake decreased and, after 8 weeks, the mean body weights of woodchucks treated with FIAU were significantly lower than controls. Anorexia, weight loss, muscle wasting, and lethargy became progressively severe, and all FIAU-treated woodchucks died or were euthanized 78 to 111 days after treatment began. Hepatic insufficiency (hyperbilirubinemia, decreased serum fibrinogen, elevated prothrombin time), lactic acidosis, and hepatic steatosis were characteristic findings in the final stages of FIAU toxicity in woodchucks. The syndrome of delayed toxicity in woodchucks was similar to that observed previously in humans treated with FIAU, suggesting that the woodchuck should be valuable in future investigations of the molecular mechanisms of FIAU toxicity in vivo and for preclinical toxicological evaluation of other nucleoside analogs before use in patients.
Assuntos
Antivirais/uso terapêutico , Arabinofuranosiluracila/análogos & derivados , Hepatite B/tratamento farmacológico , Animais , Anorexia/induzido quimicamente , Antivirais/efeitos adversos , Arabinofuranosiluracila/efeitos adversos , Arabinofuranosiluracila/farmacocinética , Arabinofuranosiluracila/uso terapêutico , Portador Sadio/virologia , DNA Viral/análise , Hepatite B/sangue , Hepatite B/patologia , Antígenos do Núcleo do Vírus da Hepatite B/análise , Vírus da Hepatite B/genética , Vírus da Hepatite B/fisiologia , Fígado/metabolismo , Fígado/patologia , Marmota , Músculos/efeitos dos fármacos , Fases do Sono , Fatores de Tempo , Replicação Viral/efeitos dos fármacosRESUMO
Portal hypertension can develop from any disorder that obstructs portal blood flow and may cause ascites in young dogs. Anomalous hepatic arteriovenous (AV) connections are rare but should be suspected in any young dog with portal hypertension or ascites. All previous reports of dogs with hepatic AV fistulae have documented macroscopic connections between the arterial and venous systems. Identical clinical signs and histopathologic findings can develop in dogs in which a macroscopic hepatic AV connection cannot be detected. Microscopic AV connections may be responsible for clinical signs in these dogs.
Assuntos
Fístula Arteriovenosa/veterinária , Doenças do Cão/etiologia , Artéria Hepática/anormalidades , Fígado/irrigação sanguínea , Veia Porta/anormalidades , Animais , Fístula Arteriovenosa/complicações , Fístula Arteriovenosa/diagnóstico por imagem , Malformações Arteriovenosas/complicações , Malformações Arteriovenosas/patologia , Malformações Arteriovenosas/veterinária , Ascite/etiologia , Ascite/veterinária , Doenças do Cão/diagnóstico por imagem , Cães , Feminino , Artéria Hepática/diagnóstico por imagem , Hipertensão Portal/complicações , Hipertensão Portal/etiologia , Hipertensão Portal/veterinária , Fígado/anormalidades , Fígado/patologia , Microcirculação/anormalidades , RadiografiaRESUMO
Acute fulminant hepatic necrosis was associated with repeated oral administration of diazepam (1.25 to 2 mg, PO, q 24 or 12 h), prescribed for behavioral modification or to facilitate urination. Five of 11 cats became lethargic, atactic, and anorectic within 96 hours of initial treatment. All cats became jaundiced during the first 11 days of illness. Serum biochemical analysis revealed profoundly high alanine transaminase and aspartate transaminase activities. Results of coagulation tests in 3 cats revealed marked abnormalities. Ten cats died or were euthanatized within 15 days of initial drug administration, and only 1 cat survived. Histologic evaluation of hepatic tissue specimens from each cat revealed florid centrilobular hepatic necrosis, profound biliary ductule proliferation and hyperplasia, and suppurative intraductal inflammation. Idiosyncratic hepatotoxicosis was suspected because of the rarity of this condition. Prior sensitization to diazepam was possible in only 1 cat, and consistent risk factors that could explain susceptibility to drug toxicosis were not identified. On the basis of the presumption that diazepam was hepatotoxic in these cats, an increase in serum transaminase activity within 5 days of treatment initiation indicates a need to suspend drug administration and to provide supportive care.
Assuntos
Ansiolíticos/efeitos adversos , Doenças do Gato/induzido quimicamente , Diazepam/efeitos adversos , Encefalopatia Hepática/veterinária , Relaxantes Musculares Centrais/efeitos adversos , Transtornos Urinários/veterinária , Animais , Ansiolíticos/uso terapêutico , Doenças do Gato/tratamento farmacológico , Doenças do Gato/terapia , Gatos , Diazepam/uso terapêutico , Feminino , Encefalopatia Hepática/induzido quimicamente , Encefalopatia Hepática/terapia , Fígado/patologia , Masculino , Relaxantes Musculares Centrais/uso terapêutico , Necrose/veterinária , Transtornos Urinários/tratamento farmacológicoRESUMO
Hepatoportal microvascular dysplasia (MVD), a congenital disorder of the hepatic vasculature, is described in a kindred of Cairn Terrier dogs. Cairn Terrier dogs (n = 165) were evaluated using the serum bile acid test. Affected dogs, identified by abnormal fasting or postprandial serum bile acid concentrations, were divided into 2 groups. Group 1 dogs (n = 147) were used for pedigree analysis. Group 2 dogs (n = 18) were characterized on the basis of history, physical examination, clinicopathologic studies, diagnostic imaging of the liver and portal circulation, and hepatic histopathology. Group 2 contained control dogs (n = 2), dogs with hepatoportal MVD (n = 11), and dogs with macroscopic portosystemic vascular anomalies (PVSA) (n = 5). With the exception of high serum bile acid concentrations, dogs with hepatoportal MVD were indistinguishable from control dogs on the basis of history, physical examination, clinicopathologic findings, survey abdominal radiography, abdominal ultrasound, or transcolonic scintigraphy. Contrast portography in dogs with MVD revealed abnormalities of terminal twigs of the portal vasculature with out large intrahepatic or extrahepatic shunting vessels. Histopathologic abnormalities in dogs with hepatoportal MVD were similar to those reported for dogs with PSVA. Pedigree analysis suggested an autosomal inheritance for MVD. Dogs with MVD had high serum bile acid concentrations, abnormal indocyanine green clearance, and hepatic pathology suggestive of PSVA, but they lacked characteristic clinical findings of PSVA. The clinical significance of MVD is unclear. Dogs with MVD were clinically normal when evaluated but long-term follow-up is not yet available. Dogs with hepatoportal MVD should be identified at an early age to avoid confusion in future diagnostic evaluations.
Assuntos
Cães/anormalidades , Circulação Hepática , Fígado/patologia , Microcirculação/anormalidades , Sistema Porta/anormalidades , Animais , Ácidos e Sais Biliares/sangue , Feminino , Fígado/fisiologia , Testes de Função Hepática , Masculino , Microcirculação/diagnóstico por imagem , Linhagem , Sistema Porta/diagnóstico por imagem , Derivação Portossistêmica Cirúrgica/veterinária , Radiografia , UltrassonografiaRESUMO
Granular cell tumor (GCT) is a morphologic designation for tumors of varied histogenesis. Most GCTs in human beings are derived from Schwann cells, and rat meningeal GCTs are believed to originate in the neural crest. Three equine pulmonary GCTs from aged horses were studied immunohistochemically with primary antibodies directed against vimentin, cytokeratins (AE1/AE3), S-100, Leu 7, desmin, and neuron-specific enolase (NSE) using a steptavidin-biotin procedure. All three tumors stained similarly with strong and diffuse staining of neoplastic cells for vimentin and S-100 and negative staining with all other antibodies. On the basis of the immunohistochemical results and the previously described histologic and ultrastructural characteristics, equine pulmonary GCT is designated as neural crest and possibly Schwann cell derived, similar to GCT in rats and human beings.
Assuntos
Tumor de Células Granulares/veterinária , Doenças dos Cavalos/diagnóstico , Neoplasias Pulmonares/veterinária , Animais , Transformação Celular Neoplásica/patologia , Desmina/análise , Feminino , Tumor de Células Granulares/química , Tumor de Células Granulares/diagnóstico , Doenças dos Cavalos/metabolismo , Cavalos , Imuno-Histoquímica , Queratinas/análise , Pulmão/química , Pulmão/metabolismo , Pulmão/patologia , Neoplasias Pulmonares/química , Neoplasias Pulmonares/diagnóstico , Fosfopiruvato Hidratase/análise , Células de Schwann/patologia , Vimentina/análiseAssuntos
Carcinoma/veterinária , Doenças dos Cavalos/etiologia , Hipercalcemia/veterinária , Neoplasias Pulmonares/veterinária , Policitemia/veterinária , Animais , Carcinoma/complicações , Carcinoma/secundário , Feminino , Doenças dos Cavalos/patologia , Cavalos , Hipercalcemia/etiologia , Pulmão/diagnóstico por imagem , Neoplasias Pulmonares/complicações , Neoplasias Pulmonares/secundário , Neoplasias Musculares/complicações , Neoplasias Musculares/secundário , Neoplasias Musculares/veterinária , Policitemia/etiologia , RadiografiaAssuntos
Encefalopatias/diagnóstico , Doenças dos Bovinos/diagnóstico , Doenças das Cabras/diagnóstico , Listeria monocytogenes/isolamento & purificação , Listeriose/veterinária , Doenças dos Ovinos/diagnóstico , Animais , Antígenos de Bactérias/análise , Encefalopatias/microbiologia , Bovinos , Doenças dos Bovinos/microbiologia , Formaldeído , Doenças das Cabras/microbiologia , Cabras , Técnicas Imunoenzimáticas/veterinária , Listeria monocytogenes/imunologia , Listeriose/diagnóstico , Listeriose/microbiologia , Inclusão em Parafina/veterinária , Estudos Prospectivos , Estudos Retrospectivos , Sensibilidade e Especificidade , Ovinos , Doenças dos Ovinos/microbiologia , Fatores de Tempo , Fixação de Tecidos/veterináriaRESUMO
Assays were validated for the measurement of urinary concentrations of cortisol and creatinine in domestic ferrets (Mustela putorius furo). Urinary concentrations of cortisol and creatinine and the calculated urinary cortisol:creatinine ratio (UCCR) values were determined for 29 clinically normal female ferrets, 22 clinically normal male ferrets, and 12 ferrets with adrenal gland tumors. The UCCR values for the 51 clinically normal ferrets ranged from 0.04 x 10(-6) to 1.66 x 10(-6), with a median value of 0.22 x 10(-6). The UCCR values were significantly (P < or = 0.01) higher in the 12 ferrets with adrenal tumors, with a range of 0.5 x 10(-6) to 60.13 x 10(-6) and a median of 5.98 x 10(-6). We concluded that determination of UCCR values was useful in the diagnosis of hyperadrenocorticism associated with adrenal neoplasia in domestic ferrets.
Assuntos
Hiperfunção Adrenocortical/veterinária , Creatinina/urina , Furões/urina , Hidrocortisona/urina , Adenocarcinoma/complicações , Adenocarcinoma/veterinária , Adenoma/complicações , Adenoma/veterinária , Neoplasias das Glândulas Suprarrenais/complicações , Neoplasias das Glândulas Suprarrenais/veterinária , Hiperfunção Adrenocortical/diagnóstico , Hiperfunção Adrenocortical/etiologia , Hiperfunção Adrenocortical/urina , Animais , Reações Cruzadas , Feminino , Masculino , Radioimunoensaio/veterinária , Valores de ReferênciaRESUMO
Adrenocortical tumors were diagnosed in 5 adult spayed ferrets. Four ferrets had bilaterally symmetrical alopecia of the caudal femoral region, abdomen, and tail, and 1 had alopecia of the distal limbs and feet. All 5 ferrets had vulvar swelling. During abdominal ultrasonography, irregular masses, believed to involve the adrenal glands, were seen in all 5 ferrets. Unilateral adrenalectomy was performed successfully in each ferret by use of ventral midline celiotomy. On histologic examination of biopsy samples, 4 ferrets were found to have adrenocortical adenomas, and 1 ferret was found to have an adrenocortical adenocarcinoma. All clinical signs resolved after adrenalectomy, suggesting that the adrenocortical tumors had been secreting adrenocortical hormones.
Assuntos
Neoplasias do Córtex Suprarrenal/veterinária , Adrenalectomia/veterinária , Adenoma Adrenocortical/veterinária , Carcinoma Adrenocortical/veterinária , Furões , Neoplasias do Córtex Suprarrenal/complicações , Neoplasias do Córtex Suprarrenal/metabolismo , Neoplasias do Córtex Suprarrenal/cirurgia , Adenoma Adrenocortical/complicações , Adenoma Adrenocortical/metabolismo , Adenoma Adrenocortical/cirurgia , Carcinoma Adrenocortical/complicações , Carcinoma Adrenocortical/metabolismo , Carcinoma Adrenocortical/cirurgia , Alopecia/etiologia , Alopecia/veterinária , Animais , Edema/etiologia , Edema/veterinária , Feminino , Hemorragia/etiologia , Hemorragia/veterinária , Hidrocortisona/metabolismo , Cuidados Pós-Operatórios/veterinária , Doenças Vaginais/etiologia , Doenças Vaginais/veterinária , Doenças da Vulva/etiologia , Doenças da Vulva/veterináriaRESUMO
Five 3-month-old foals presenting with fever and respiratory disease were found to have pulmonary abscesses with patchy to diffuse alveolar and interstitial pneumonia on post-mortem examination. All affected foals had evidence of Rhodococcus equi infection and had few to abundant Pneumocystis carinii cysts in the sections of affected lung. Of the 5 foals examined radiographically, 3 had a distinct reticulonodular (miliary) pattern which may aid in the ante-mortem diagnosis of P. carinii pneumonia (PCP). Leukocyte counts of foals with PCP were significantly greater than in the control group of foals with uncomplicated bacterial pneumonia. Foals with PCP tended to be more tachypnoeic than the control foals and 4 of the 5 PCP+ foals appeared dyspnoeic before death. The ante-mortem recognition of PCP may be expedited by bronchoalveolar lavage and successful treatment of foals with PCP may require the administration of adequate levels of potentiated sulphonamides.
Assuntos
Doenças dos Cavalos/diagnóstico , Pulmão/patologia , Pneumonia por Pneumocystis/veterinária , Infecções por Actinomycetales/complicações , Infecções por Actinomycetales/veterinária , Animais , Feminino , Doenças dos Cavalos/sangue , Cavalos , Contagem de Leucócitos/veterinária , Pulmão/diagnóstico por imagem , Pulmão/parasitologia , Masculino , Pneumocystis/isolamento & purificação , Pneumonia/complicações , Pneumonia/veterinária , Pneumonia por Pneumocystis/sangue , Pneumonia por Pneumocystis/complicações , Pneumonia por Pneumocystis/diagnóstico , Radiografia , Respiração , Rhodococcus equiRESUMO
A 9-year-old Quarter Horse mare was examined because of progressive weight loss, weakness, muscle atrophy and tremors, and behavioral change. Selenium and glutathione peroxidase assays, blood lead analysis, erythrocyte transketolase analysis, pseudorabies and Borrelia burgdorferi serology, electromyography, and CSF analysis were performed. Motor neuron degeneration was diagnosed by microscopic examination of neural tissues. The cause of the disease was not substantiated, but several possibilities were excluded via diagnostic testing. Diagnosis of motor neuron degeneration in horses may be made from an accurate history, thorough neurologic examination, and ancillary testing. In particular, antemortem diagnosis may be based on finding scattered angular atrophy of predominantly type-1 or of type-1 and -2 skeletal muscle fibers in frozen sections of muscle biopsy specimens.
Assuntos
Doenças dos Cavalos/patologia , Doença dos Neurônios Motores/veterinária , Neurônios Motores/patologia , Degeneração Neural , Animais , Axônios/patologia , Feminino , Secções Congeladas , Gânglios Espinais/patologia , Doenças dos Cavalos/fisiopatologia , Cavalos , Doença dos Neurônios Motores/patologia , Doença dos Neurônios Motores/fisiopatologia , Músculos/patologia , Atrofia Muscular/patologia , Atrofia Muscular/veterinária , Prognóstico , Medula Espinal/patologiaRESUMO
Administration of trimethoprim-sulfadiazine in a dog was associated with vomiting, inappetence, and icterus, and high values of alanine transaminase, aspartate transaminase, alkaline phosphatase, gamma-glutamyltransferase, and total bilirubin concentration. The clinical signs and biochemical abnormalities resolved after discontinuation of the treatment. Histologic examination of sections from a liver biopsy specimen revealed moderate, predominantly portal hepatitis with cholestasis.
Assuntos
Doenças do Cão/induzido quimicamente , Hepatopatias/veterinária , Pioderma/veterinária , Sulfadiazina/efeitos adversos , Trimetoprima/efeitos adversos , Animais , Doença Hepática Induzida por Substâncias e Drogas , Doenças do Cão/tratamento farmacológico , Cães , Combinação de Medicamentos , Seguimentos , Fígado/efeitos dos fármacos , Masculino , Pioderma/tratamento farmacológico , Sulfadiazina/uso terapêutico , Trimetoprima/uso terapêuticoRESUMO
Morphological information is presented for syntype material of the etiologic agent of equine protozoal myeloencephalitis, Sarcocystis neurona. A clinical description of the horse from which the organism was isolated and the methodology used to immunosuppress the horse in an attempt to increase parasite numbers are also given. The description includes microscopic details observed both with light and transmission electron microscopy. Mainly stages from tissue are illustrated, but information is also presented on the development of the organism after inoculation onto monolayers of bovine monocytes. It is believed that the large numbers of organisms observed in this horse were due to its having not received prior treatment with trimethoprimsulphonamide and the large amounts of corticosteroids that were administered in order to facilitate isolation of the pathogen.
Assuntos
Encefalomielite/veterinária , Doenças dos Cavalos/parasitologia , Sarcocystis/ultraestrutura , Sarcocistose/veterinária , Medula Espinal/parasitologia , Animais , Tronco Encefálico/patologia , Encefalomielite/parasitologia , Histocitoquímica , Cavalos , Terapia de Imunossupressão/veterinária , Masculino , Microscopia Eletrônica , Sarcocystis/isolamento & purificação , Sarcocistose/parasitologia , Medula Espinal/patologia , Medula Espinal/ultraestrutura , Telencéfalo/patologiaRESUMO
Cutaneous plasmacytomas associated with local deposition of amyloid were diagnosed by light microscopy in a series of six older dogs (mean age 10.7 years) consisting of two Cocker Spaniels, a Poodle, a Weimeraner, and two mixed-breed dogs. The neoplasms occurred on the digits (2 dogs), forelimb (2 dogs), lip (1 dog), and ear (1 dog). In most cases, groups of neoplastic plasma cells were widely separated by large homogeneous islands of amyloid. The neoplastic cells had characteristic plasmacytoid features, but the degree of pleomorphism varied greatly between different neoplasms. In four of the six tumors, the diagnosis of plasmacytoma was confirmed by the demonstration of a monoclonal plasma cell population using immunofluorescent staining for anti-canine immunoglobulins. In these tumors, the neoplastic cells reacted with only one class of immunoglobulins (IgG). The amyloid did not react with any of the reagents used. The suspicion that the amyloid was of immunoglobulin origin (primary amyloid) was supported by its retention of birefringence under polarized light after treatment with potassium permanganate and staining with Congo red.
Assuntos
Amiloide/análise , Doenças do Cão/patologia , Plasmocitoma/veterinária , Neoplasias Cutâneas/veterinária , Animais , Vermelho Congo , Cães , Orelha Externa , Feminino , Imunofluorescência , Seguimentos , Membro Anterior , Humanos , Imunoglobulinas/análise , Lábio , Masculino , Microscopia Eletrônica , Microscopia de Fluorescência , Plasmocitoma/química , Plasmocitoma/patologia , Permanganato de Potássio , Neoplasias Cutâneas/química , Neoplasias Cutâneas/patologia , Coloração e Rotulagem , Dedos do PéRESUMO
A 12-year-old Morgan gelding was examined for colic of 3 days duration. Signs of depression, colic, diarrhea, and endotoxemia persisted despite aggressive medical therapy and surgical exploration. Culture results from gastric fluid and feces yielded many colonies of Clostridium perfringens. This organism also was recovered from peritoneal fluid 10 days after admission; consequently, the horse was euthanized. At necropsy, a localized gas-filled, necrotic stomach wall was found; many mucosal and submucosal gas blebs were visible. Culture of this tissue yielded Clostridium perfringens. Emphysematous gastritis is a fulminant infection of the stomach wall caused by gas-forming organisms that gain access to the submucosa via mucosal defects such as ulcers. This condition has been reported infrequently in people, and the case reported herein represents the first instance of emphysematous gastritis in the horse.