RESUMO
We analyzed variability of the expression of three reference genes in biopsy samples of the olfactory epithelium obtained from healthy volunteers. The expression of B2M, HPRT1, and CASC3 genes was analyzed by real-time PCR. The pairs of genes B2M-HPRT1 and B2M-CASC3 were found to possess minimum individual variability of expression and can be reliable candidates for the reference genes in analysis of gene expression in neural cells.
Assuntos
Mucosa Olfatória/metabolismo , Adolescente , Adulto , Biópsia , Feminino , Perfilação da Expressão Gênica , Regulação Neoplásica da Expressão Gênica/genética , Humanos , Hipoxantina Fosforribosiltransferase/metabolismo , Técnicas In Vitro , Masculino , Pessoa de Meia-Idade , Proteínas de Neoplasias/metabolismo , Proteínas Nucleares/metabolismo , Proteínas de Ligação a RNA , Adulto JovemRESUMO
The pioneer overall ultrastructural and immune-enzymatic evaluation of the secretory activity of atrial myoendocrine cells in WAG strain (control) and in ISIAH rats with inherited stress induced arterial hypertension has been carried out. It was revealed that under basal conditions the cardiac hormone synthesis, storage and secretion in myoendocrine cells of hypertensive rats were certainly intensified, and the atrial natriuretic peptide (ANP) blood concentration was reliably higher than in normotensive WAG rats. All rats demonstrated the unidirectional reaction during the subchronic restraint stress: the ANP release was depressed, the peptides accumulated in the cardiomyocyte numerous large secretory granules, and ANP blood concentration 6 times decreased, while interline differences preserved. It is concluded that the cardiac natriuretic peptides as a hypotensive chain of the hemodynamic regulation, compensatory response to the development of inherited arterial hypertension and participate in the realization of stress reactions.
Assuntos
Átrios do Coração/metabolismo , Hipertensão/fisiopatologia , Miócitos Cardíacos/metabolismo , Estresse Psicológico/fisiopatologia , Animais , Fator Natriurético Atrial/sangue , Pressão Sanguínea , Átrios do Coração/ultraestrutura , Hipertensão/patologia , Hipertensão/psicologia , Imobilização , Masculino , Tamanho do Órgão , Ratos , Estresse Psicológico/psicologiaRESUMO
The up-to-day world-wide data about the structure, distribution, and physiological effects of the most poorly known among the natriuretic peptides--the C-type (CNP)--are summarized in the review. Despite its name, this peptide does not stimulate sodium excretion but shares the prominent vasodilating and antyproliferating effects in different organs and tissues. The special emphasis is attended to CNP functions in central nervous system. The information about the peptide molecular biology, including intracellular processing, blood peptide concentration, specific receptors structure, and signaling pathways in target cells is presented.
Assuntos
Sistema Nervoso Central/metabolismo , Peptídeo Natriurético Tipo C/genética , Sódio/metabolismo , Vasodilatação/fisiologia , Animais , Sistema Nervoso Central/fisiologia , Humanos , Inflamação/fisiopatologia , Camundongos , Peptídeo Natriurético Tipo C/sangue , Peptídeo Natriurético Tipo C/química , Ratos , Receptores do Fator Natriurético Atrial/genética , Receptores do Fator Natriurético Atrial/metabolismo , Transdução de SinaisRESUMO
Male ISIAH rats with inherited stress-induced arterial hypertension (BP 174.0 ± 1.3 mm Hg) received antagonist of angiotensin II receptors losartan in a dose of 10 mg/kg/day for 16 days. Ultrastructural study of the right atrium showed signs of dramatic and pronounced inhibition of synthesis of the natriuretic peptides (changes in the composition of secretory granules and decrease in their population density and size) the atrial myocytes against the background of persistent BP decrease in hypertensive rats to 142.0 ± 4.2 mm Hg. We concluded that myoendocrine cells in rats with stable hypertension retain ability to respond adequately to distention of the atria with blood.
Assuntos
Antagonistas de Receptores de Angiotensina/uso terapêutico , Anti-Hipertensivos/uso terapêutico , Fator Natriurético Atrial/metabolismo , Hipertensão/tratamento farmacológico , Losartan/uso terapêutico , Animais , Coração/efeitos dos fármacos , Hipertensão/metabolismo , Masculino , Miócitos Cardíacos/efeitos dos fármacos , Miócitos Cardíacos/metabolismo , Peptídeos Natriuréticos/metabolismo , RatosRESUMO
It is well known now that atrial cardiomyocytes carry out both contractile and endocrine activities--they synthesize, accumulate in specific secretory granules and release the natriuretic peptides. The main physiological effects of natriuretic peptides are antagonistic to the renin-angiotensin-aldostrol system, but their role in the development of hypertension is still disputable. The aim of this investigation is to estimate using electron microscopy the secretory activities of atrial myoendocrine cells in rats with inherited stress-induced arterial hypertension (ISIAH stain). It has been shown that myoendocrine cells in the ISIAH rats with arterial pressure about 180 mm Hg reveal morphological features of increased synthesis, extra accumulation and release of natriuretic peptides compared with normotensive control rats. In the ISIAH rats treated with losartan (angiotensin II receptor blocker) and therefore having a sustained decrease in arterial pressure to 140 mm Hg, changes in granular pool composition, reduction of the number and diameter of the secretory granules, reduction of Golgi complexes, and increased intracellular degradation of secretory stores were found in the myoendocrine cells. At the same time the marked capillary hyperemia and interstitial edema in the myocardium were observed. Thus, in rats with severe inherited hypertension, the secretory activity of heart myoendocrine cells is sharply increased and directly depends on the arterial blood pressure level. This proves that natriuretic peptides actively participate in the regulation of hemodynamics during with cardiovascular pathology.