Resistant starch formation mechanism of amylosucrase-modified starches with crystalline structure enhanced by hydrothermal treatment.

The aim of this study was to reveal the underlying mechanism contributing towards the formation of resistant starch (RS) in amylosucrase-modified starches with crystalline structure enhanced by hydrothermal treatment. The branch chains of waxy corn starch were continuously elongated by amylosucrase, and the retrogradation of elongated starches with weight-average chain length (CLw¯) of 27.0-37.6 yielded B-type retrograded starches (MSs) with crystallinity increasing from 33.1 % (MS-5) to 41.4 % (MS-30). Increasing the starch crystallinity improved the content of RS from 6.7 % of MS-5 to be as much as 41.0 % of MS-30. During the hydrothermal treatment, MS-5 with CLw¯ of 27.0 favored the B â†’ A allomorphic transition, leading to the decreased starch digestibility. Moreover, the hydrothermal treatment facilitated the assembly of double helices to increase starch crystallinity, which further increased the content of RS. The findings of the present study may assist the preparation of functional starches with controllable digestibility.

Hydrothermal induced B → A allomorphic transition in retrograded starches with side chains elongated by amylosucrase to different lengths.

In this study, chain-elongated starches were modified with hydrothermal treatment to produce hydrothermal-treated starches with different crystalline structures. All chain-elongated starches showed a B-type crystalline structure and the retrogradation of long branch chains accelerated the formation of starch crystallites. The hydrothermal treatment preserved the granular structure of starches but facilitated the rearrangement of starch chains to generate crystallites. Starches with short chain length favored the B â†’ A allomorphic transition during the hydrothermal treatment. A longer chain length of starch led to greater stability of double helices and accordingly inhibited the B â†’ A allomorphic transition, resulting from the hydrogen bonding along with the direction of helix restrained the displacement of the helix. The longer double helices resulted in higher gelatinization temperature of the chain-elongated starches. Moreover, the gelatinization temperature of the starches was further enhanced by the hydrothermal treatment, and both increased crystallinity and B â†’ A allomorphic transition contributed to the improved thermal stability of the hydrothermal-treated starches.