RESUMO
BACKGROUND: Human papillomaviruses (HPV) may play an important role as one of the possible etiologies of oral squamous cell carcinoma (OSCC). The present study aimed to investigate the association between HPV and OSCC in young Japanese patients by examining the presence of HPV DNA and surrogate markers in OSCC tissues. MATERIALS AND METHODS: Forty young patients with OSCC whose surgical specimens were available were analyzed and compared with 40 patients randomly recruited from a pool of patients aged >40 years. HPV DNA was detected using the polymerase chain reaction-based AMPLICOR(®) HPV test, and surrogate markers of HPV infection were analyzed using immunohistochemical techniques to detect p16(INK4a) and p53. RESULTS: Only two (5%) young patients and one (2.5%) older patient were positive for HPV DNA. p16(INK4a) overexpression was identified in six (15%) young patients. p53 staining levels were not high in tissues of most young patients (27 patients, 67.5%). HPV DNA status did not significantly correlate with p16(INK4a) expression levels. Profiles of increased levels of p16(INK4a) expression with diminished levels of p53 staining were not associated with the presence of HPV DNA. The combined p53 with p16(INK4a) profiles were significantly correlated with alcohol consumption in younger patients (p=0.006). CONCLUSIONS: RESULTS of the present study indicate that HPV is less likely to cause OSCC in young Japanese patients, and the p16(INK4a) expression level is not an appropriate surrogate marker for HPV infection in OSCC.
Assuntos
Carcinoma de Células Escamosas/virologia , Inibidor p16 de Quinase Dependente de Ciclina/biossíntese , DNA Viral/sangue , Neoplasias Bucais/virologia , Infecções por Papillomavirus/etiologia , Adulto , Fatores Etários , Consumo de Bebidas Alcoólicas , Biomarcadores Tumorais/sangue , Inibidor p16 de Quinase Dependente de Ciclina/genética , Feminino , Testes de DNA para Papilomavírus Humano , Humanos , Japão , Masculino , PapillomaviridaeRESUMO
Polymorphisms in the promoter and 5' untranslated region of vascular endothelial growth factor (VEGF) have been associated with VEGF levels. To investigate the role of VEGF polymorphisms in breast cancer, the VEGF 2578C/A, 1498C/T, 1154G/A and 634G/C polymorphisms were genotyped in 483 breast cancer patients and 524 healthy controls. VEGF mRNA levels in breast cancer tissue were determined using semiquantitative RTPCR. The genotypes, 634G/C and 634C/C, were associated with an increased risk for breast cancer when compared with the 634G/G genotype. The VEGF 634G/C genotype was associated with tumor size >20 mm, perineural invasion and stage IIIV. Individuals with 634C/C had lower diseasefree survival. Patients with the VEGF 634C/C genotype exhibited the highest VEGF mRNA levels. High VEGF mRNA expression correlated with tumor size >20 mm, presence of lymphovascular invasion and axillary nodal metastasis. These observations suggested that VEGF 634G/C polymorphisms have a significant role in breast cancer susceptibility and aggressiveness.