[Features of the Udmurt mitochondrial gene pool in relation to tribal structure].

We present novel data on mitochondrial DNA polymorphism in the Udmurt population, which represents a Finno-Ugric ethnos residing in the Volga-Ural region. Our analysis of the Udmurt mtDNA polymorphisms have shown that neighboring ethnoses had almost no effecton formation of the Udmurtian intraethnic diversity. These results strongly indicate that genetic differences in the Udmurtian population are determined by their tribal structure rather than their geographic location.

[Some features of mitochondrial gene pool of Maeotis in light of their relation to Cis-Asov nomads].

New data on mitochondrial gene pool polymorphism of Maeotis (1st-3rd centuries CE) in the light of their relation with Sarmatian nomads are presented. Maeotis are more genetically various, compared to Sarmatians; both the age of Maeotian gene pool and their close interactions with neighboring tribes can be reasons for this. The study of relationships of Maeotis and Sarmatians suggests an intensive gene interchange between them, which influences significantly on the formation of the Maeotian gene pool.

[Mitochondrial DNA variability in populations and ethnic groups of Tatars of the Tobol-Irtysh basin].

The data on mitochondrial DNA diversity in seven local populations (villages) and four territorial groups of Tatars of the Tobol-Irtysh basin are presented. In the Turkic-speaking populations from the Tobol and Irtysh river basins, high levels of intergroup and interpopulation mtDNA variation were observed. It was demonstrated that genetic diversity of the territorial groups of Tatars of the Tobol-Irtysh basin resulted from various interethnic relationships and different ethnic components integrated into these groups.

[Mitochondrial DNA diversity in Kazym Khanty].

New data on mitochondrial DNA polymorphism in the representatives of Kazym territorial group of Northern Khanty are presented. MtDNA diversity observed in Kazym Khanty was compared with that in Khanty from Shuryshkarskii raion of Yamalo-Nenets Autonomous Okrug.

[Mitochondrial DNA diversity in Siberian Tatars of the Tobol-Irtysh basin].

Data on the variation of the nucleotide sequence of hypervariable segment I (HVSI) and restriction fragment length polymorphism (RFLP) of the coding region of mitochondrial DNA (mtDNA) have been used to characterize the mitochondrial gene pool of Siberian Tatars of the Tobol-Irtysh basin (N = 218), one of three geographic/linguistic groups of Siberian Tatars. The gene pool of Siberian Tatars has been shown to contain both Asian and European mtDNA lineages at a ratio of 1 : 1.5. The mtDNA diversity of Siberian Tatars is substantially higher than that of other Turkic-speaking populations of North and Central Asia. The position of the mitochondrial gene pool of Tatars of the Tobol-Irtysh basin in the genetic space of northern Eurasia populations has been determined.

[Mitochondrial DNA polymorphism in Russian population form five oblasts of the European part of Russia].

New data on mitochondrial DNA polymorphism among Russian population from five oblasts, located within the main ethnic area of Russians, specifically, Ryazan' oblast, Ivanovo oblast, Vologda oblast, Orel oblast, and Tambov oblast (N = 177) are presented. RFLP analysis of the mtDNA coding region showed that most of the mtDNA diversity in the populations examined could be described by main European haplogroups H, U, T, J, K, I, V, W, and X. Haplogroup frequency distribution patterns in the populations of interest were analyzed in comparison with the European and Uralic populations. Based on the haplogroup frequencies, the indices of intraethnic population diversity, Wright's F(st) statistics, and the values of squared deviation from the mean, as well as genetic distances between Russians and European and Uralic populations were estimated. Analysis of these indices along with the anthropological data provided identification of a number of regional groups within the populations examined, which could either result from the interaction of ancient Slavs with different non-Slavic tribes, or could be caused by the ethnic heterogeneity of the ancient Slavs themselves.

[Molecular genetic characteristics of the neolithic population of the Baikal region: RFLP analysis of the ancient mitochondrial DNA from osseous remains found in the Ust-Ida I burial ground]. / Molekuliarno-geneticheskaia kharakteristika neoliticheskoi populiatsii Pribaikal'ia. Analiz PDRF drevnei mtDNK iz ostankov v Mogil'nike ust'-Ida I.

Nineteen mtDNA samples from osseous remains found in the Ust-Ida I burial ground (middle Angara River) were analyzed. An ancient population dated back to 4020-3210 B.C. by radiocarbon (14C) analysis and archeologically assigned to the Neolithic Isakovo culture of the Baikal region was described in terms of molecular genetics. Data on restriction-site polymorphisms in fragment 16,106-16,545 of the mtDNA D-loop were obtained for seven restriction endonucleases. On the basis of these data, the mitotypic structure and nucleotide diversity of the ancient population were determined. The molecular genetic characteristics of the Neolithic population were compared to the modern populations of Siberia, Mongolia, and Urals. The data obtained indicate that the studied Baikal Neolithic population was ancestral for the modern indigenous Siberian population. The time of divergence of the three regional populations (5572 years ago) was estimated from the genetic distances between the Neolithic and modern Siberian populations, assuming that the average rate of nucleotide substitution was constant. This estimation agrees with the results of the radiocarbon dating (5542-5652 years ago). The fact that the studied samples were 14C-dated allowed the rate of nucleotide substitution in the studied region of mtDNA D-loop to be directly determined.

[A computer-assisted approach to the study of nucleic acid polymorphism]. / Komp'iuternyi podkhod k izucheniiu polimorfizma nukleinovykh kislot.

A computer software package has been created that assists studying restriction fragment length polymorphism (RFLP). The software simulates and analyzes the results of amplification by means of polymerase chain reaction (PCR) followed by restriction of amplification products, as well as the results of nucleic acid electrophoresis.

[Gene pool of residents of northeastern Eurasia in light of data on polymorphism of mitochondrial DNA. I. New data on polymorphism of restriction sites of the D-loop of mtDNA in aboriginal populations of the Caucasus and Siberia]. / Genofond naseleniia severo-vostochnoi evrazii v svete dannykh o polimorfizm mitokhondrial'nom DNA. I. Novye dannye o polimorfizme saitov restriktsii D-petli mtDNK v korennom naselenii Kavkaza i Sibiri.

New data about eleven polymorphic sites of restriction in the D-loop of mtDNA revealed with the Ava II, BamH I, EcoR V, Hae III, Kpn I, Rsa I, and Sau3A I restriction enzymes in native populations of the Caucasus (N = 40) and Siberia (N = 44) are presented. A comparison of these groups with each other and with common data for western and eastern Europe as well as for central and eastern Asia reveals a large similarity of all the population groups of Eurasia in respect to the frequency of each polymorphic site separately. The interpopulation differentiation of frequency of polymorphic restriction sites in Eurasia is low and identical at such different hierarchical levels of population structure as ethnic group and extended regional population group. An analysis of differences between sites in the frequency of the presence of restriction sites for BamH I, EcoR V, Hae III, and Sau3A I and the absence of sites for Ava II, Kpn I, and Rsa I at definite loci of the nucleotide chain allowed determination of the region of equilibrium between direct, site-generating, and reverse mutations to be 0.43q. Among eleven polymorphic restriction sites analyzed here, only polymorphism in the Hae III site at the position 16,517 in the population of Eurasia was characterized with frequencies close to equilibrium that determined the extremely large evolutionary age of this polymorphism.