RESUMO
Biofilms can enhance the sorption of heavy metals onto microplastic (MP) surfaces. However, most research in this field relies on laboratory experiments and neglects metal fractions and seasonal variations. Further studies of the metal/biofilm interaction in the aquatic environment are essential for assessing the ecological threat that MPs pose. The present study used in situ experiments in an environment conducive to biofouling (Vistula Lagoon, Baltic Sea). The objective was to investigate the sorption of mercury and its fractions (thermodesorption technique) in MP (polypropylene-PP, polystyrene-PS, polylactide-PLA) biofilms and natural matrices across three seasons. After one month of incubation, the Hg concentrations in MP and natural substratum (gravel grains-G) biofilms were similar (MP: 145 ± 45 ng/g d.w.; G: 132 ± 23 ng/g d.w.) and approximately twofold those of suspended particulate matter (SPM) (63 ± 27 ng/g d.w.). Hg concentrations in biofilms and sediments were similar, but labile fractions dominated in biofilms and stable fractions in sediments. Seasonal Hg concentrations in MP biofilms decreased over summer>winter>spring, with significant variation for mineral and loosely bound Hg fractions. Multiple regression analysis revealed that hydrochemical conditions and sediment resuspension played a crucial role in the observed variability. The influence of polymer type and morphology (pellets, fibres, aged MP) on Hg sorption in biofilms was visible only in high summer temperatures. In this season, PP fibres and aged PP pellets encouraged biofilm growth and the accumulation of labile Hg fractions. Additionally, high concentrations of mineral (stable and semi-labile) Hg fractions were found in expanded PS biofilms. These findings suggest that organisms that ingest MPs or feed on the biofilms are exposed to the adverse effects of Hg and the presence of MPs in aquatic ecosystems may facilitate the transfer of mercury within the food chain.
Assuntos
Biofilmes , Monitoramento Ambiental , Sedimentos Geológicos , Mercúrio , Microplásticos , Estações do Ano , Poluentes Químicos da Água , Microplásticos/análise , Poluentes Químicos da Água/análise , Mercúrio/análise , Sedimentos Geológicos/química , Sedimentos Geológicos/microbiologia , Material Particulado/análiseRESUMO
The Chinese mitten crab (Eriocheir sinensis) is considered one of the 100 most invasive alien species in the world. Despite this, its role in ecosystems, among others, in the trophodynamics of pollutants including mercury, is still not fully understood. Becoming an increasingly important and widespread element of the trophic chain in new areas arouses interest from humans as consumers. Hence it is important to determine the level of contaminants (including Hg) in alien species. In the present study, great attention was paid separately to the soft tissues and hard tissues of the exoskeleton, which may play an important role in the detoxification of the crab's body from toxic Hg. The study was conducted on crabs collected in 2011-2021 in the Vistula Lagoon. Concentrations of total mercury and its forms were carried out using a Direct Mercury Analyzer, DMA-80 (Milestone, Italy). The present study showed that mercury accumulation of the crab's body largely occurred through the gills, followed by the oral route. The distribution of Hg in the crab's organs was related to the trophic origin of the mercury, while halide-bound mercury and semilabile forms from the respiration (filtration) process were redistributed into the crab's exoskeleton. Male crabs, compared to females, had a higher Hg burden on internal organs such as their hepatopancreas and gonads. Hg concentration in hard tissues was closely related to the type of mineralization of the carapace. The elimination of Hg from the muscles and from the hepatopancreas into the carapace was one of the important detoxification processes of the crab's body. Thus, moulting crabs effectively remove Hg protecting its body from the neurotoxin. As a result, a smaller Hg load is biomagnified, making the crab's muscle tissue fit for human consumption. The observed decrease in Hg concentrations from 2011 to 2021, as well as the spatial variability of Hg in the crab's muscles, testify that the crab can serve as a biomonitor for ecosystem changes.