King crab gills as a new source of chitin/chitosan and protein hydrolysates.

This is the first report on the physicochemical properties of chitin obtained from gills of the king crab Paralithodes camtschaticus. In the present study, we investigated the chemical composition of red king crab gills and considered methods of its complex processing to obtain chitin and enzymatic protein hydrolysates. The gills contained approximately 21 % chitin in terms of dry matter. For the first time, the gills of the king crab were investigated as a source of chitin and chitosan. Chitin was isolated from crab gills using chemical and enzymatic methods. The physicochemical properties of chitin and chitosan from the gills were investigated. By performing infrared spectroscopy and X-ray phase analyses, the chitin present in the gills was established to be α-chitin. The physical and chemical properties (degree of deacetylation, molecular weight and crystal structure) of gill chitin and chitosan were absolutely similar to those of crab shell. Crab gills can be used as an additional source of chitin in the integrated processing of king crabs. The yield of chitin from the gills is up to 45 % of the yield of chitin from the crab carapace.

[Glycolytic activity of enzyme preparation from the red king crab (Paralithodes camtschaticus) hepatopancreas].

Enzyme preparation exhibiting glycolytic activity yielding chitooligosaccharides along with N-acetyl-D-glucosamine was obtained from the red king crab (Paralithodes camtschaticus) hepatopancreas. The results of the analysis confirmed the presence of endo- and exochitinase activities in the preparation. HPLC showed that the hydrolysis products of chitin and chitosan did not contain D(+)-glucosamine, which is indicative of the absence of deacetylase and, apparently, exochitosanase activities. A comparison of the dependence of the enzyme preparation activity on temperature and pH of the incubation medium suggests that chitinase and protease activities are exhibited by different enzymes.

[Properties of chitinolytic enzymes from the hepatopancreas of the red king crab (Paralithodes camtschaticus)].

Our study confirms the presence of chitinolytic, chitosanolytic, and deacetylase activities in the hepatopancreas of the red king crab, related to the specific diet of this species. The maximum rate of chitin/chitosan hydrolysis by an enzyme preparation from crab hepatopancreas occurs at 36.5-37.0 degrees C. Two pH optimums have been found for the enzymatic reaction under mildly alkaline and acidic conditions for both exo- and endochitinase activities. The enzyme preparation is most affine to partly deacetylated chitin with an acetylation degree within 40-50%.