Spatio-temporal assessment of soil properties immediately and eight months after a high intensity-controlled burn in the south of Spain.

In recent years, the use of fire as a means by which to manage forest ecosystems has become more frequent in Europe. Fire has a significant impact on the soil, and it is therefore necessary to understand how controlled burns affect this invaluable resource. The purpose of this study was to evaluate the main alterations in the physical-chemical and biological properties of the soil because of a high intensity-controlled burn in "Los Boquerones" area (Villaviciosa de Córdoba, Spain). Additionally, we assessed the spatial heterogeneity of the alterations of different soil properties. A grid of 12 points was established on a hillside in Sierra Morena (Córdoba). Thermocouples were placed at each point, and soil samples were collected at two depths (0-2 cm and 2-5 cm) before burning, immediately after burning and eight months later. Soil pH, electrical conductivity, nutrient content and/or availability, among others, and their spatio-temporal variations were analysed. Soil pH, increased in the first centimetres of the soil (0-2 cm) immediately after burning up to >2 units, and the increase was maintained eight months following the burn. Additionally, the high-intensity burn had a positive short-term effect on some of the soil properties, such as nutrient availability for plants, which was considerably increased. The magnitude of the alterations in the soil indicators assessed was spatially explained by the behaviour of the fire during the controlled burning. The burn also had both direct and indirect effects on soil microorganisms. In conclusion, the possible immediate and short-term effects of burning on the soil resource should be considered for a more holistic management of fire in forest ecosystems, as its functionality and capacity to provide ecosystem services is largely altered by these events as a function of their intensity.

Industrial saline wastewater in a corn-soybean rotation to enhance crop yield without compromising soil health in a subtropical soil.

The production of industrial waste has increased in the last decades along with world population. Wastes are used in agriculture as fertilizers and soil amendments depending on their composition, dynamics in soil and effects on plant growth. The aim of this study was to assess the effect of industrial saline wastewater from heparin production on soil chemistry and plant yield in a subtropical soil in Brazil. Five rates of industrial saline wastewater (0, 10, 20, 40 and 60 m3 ha-1 year-1) were applied as fertilizer in a corn -soybean rotation on an Oxisol with limited nutrient availability. Five soils sampling were done: before and after the first application of the industrial wastewater (2017, corn), before and after the second application of wastewater (2018, soybean) and two years after the first application (2019). Soil K, Ca, Mg, Na content and CEC increased immediately after the application of wastewater but they returned to former values with time due to plant uptake and lixiviation. Wastewater application significantly increased corn (all rates) and soybean (only with the highest rate) yields around 103-250% and 50%, respectively, in comparison with no wastewater application. However, the highest rate temporally increased soil Na content and electrical conductivity (up to 650 and 800%, respectively). Although nutrient uptake, chlorophyll content and corn and soybean yields were enhanced, the use of high rates of industrial saline wastewater could cause soil salinization (mainly in locations with low rainfall), affecting soil chemistry and physical parameters due to clay dispersion, and pollution or water bodies.

Combining P and Zn fertilization to enhance yield and grain quality in maize grown on Mediterranean soils.

The main aim of this study was to elucidate the effect of individual and joint fertilization with P and Zn on maize plants grown on typical Mediterranean soils with a limited Zn availability. For this purpose, we examined the effects of P and Zn fertilization individually and in combination on growth, yield and grain protein content in maize grown in pots filled with three different Mediterranean soils (LCV, FER and INM). Phosphorus and Zn translocation to grain was impaired, and aboveground dry matter and yield at harvest reduced by 8-85% (LCV and FER), in plants treated with Zn or P alone relative to unfertilized (control) plants. In contrast, joint fertilization with P and Zn enhanced translocation of these nutrients to grain and significantly increased aboveground dry matter (30% in LCV, 50% in FER and 250% in INM) and grain Zn availability in comparison with control plants. Also, joint application of both nutrients significantly increased grain P (LCV) and Zn (LCV and FER) use efficiency relative P and Zn, respectively, alone. Yield was increased between 31% in LCV and 121% in FER relative to control plants, albeit not significantly. Fertilization with P or Zn significantly influenced the abundance of specific proteins affecting grain quality (viz., storage, lys-rich and cell wall proteins), which were more abundant in mature grains from plants fertilized with Zn alone and, to a lesser extent, P + Zn. Sustainable strategies in agriculture should consider P-Zn interactions in maize grown on soils with a limited availability of Zn, where Zn fertilization is crucial to ensure grain quality.

Entomopathogenic fungi-based mechanisms for improved Fe nutrition in sorghum plants grown on calcareous substrates.

Although entomopathogenic fungi (EPF) are best known for their ability to protect crops against insect pests, they may have other beneficial effects on their host plants. These effects, which include promoting plant growth and conferring resistance against abiotic stresses, have been examined in recent years to acquire a better understanding of them. The primary purposes of the present study were (i) to ascertain in vitro whether three different strains of EPF (viz., Metarhizium, Beauveria and Isaria) would increase the Fe bioavailability in calcareous or non-calcareous media containing various Fe sources (ferrihydrite, hematite and goethite) and (ii) to assess the influence of the EPF inoculation method (seed dressing, soil treatment or leaf spraying) on the extent of the endophytic colonization of sorghum and the improvement in the Fe nutrition of pot-grown sorghum plants on an artificial calcareous substrate. All the EPFs studied were found to increase the Fe availability during the in vitro assay. The most efficient EPF was M. brunneum EAMa 01/58-Su, which lowered the pH of the calcareous medium, suggesting that it used a different strategy (organic acid release) than the other two fungi that raised the pH of the non-calcareous medium. The three methods used to inoculate sorghum plants with B. bassiana and M. brunneum in the pot experiment led to differences in re-isolation from plant tissues and in the plant height. These three inoculation methods increased the leaf chlorophyll content of young leaves when the Fe deficiency symptoms were most apparent in the control plants (without fungal inoculation) as well as the Fe content of the above-ground biomass in the plants at the end of the experiment. The total root lengths and fine roots were also increased in response to fungal applications with the three inoculation methods. However, the soil treatment was the most efficient method; thus, its effect on the leaf chlorophyll content was the most persistent, and the effects on the total root length and fine roots were the most apparent. In conclusion, EPF improved the Fe nutrition of the sorghum plants, but their effects depended on the inoculation method.

Phosphorus reduces the zinc concentration in cereals pot-grown on calcareous Vertisols from southern Spain.

BACKGROUND: Zinc deficiency, a major problem in crops grown on soils low in available Zn, is even more important in phosphorus-rich soils. This work aimed to elucidate the effects of soil P and Zn levels, and of fertilizer application, on yield and Zn concentration in cereal grains. RESULTS: Wheat and barley were successively pot-grown on 20 calcareous Vertisols low in available Zn and ranging widely in available P. Grain yield in the plants grown on the native soils was positively correlated with Olsen P but not with diethylenetriaminepentaacetic acid (DTPA)-extractable Zn except for wheat on P-rich soils. Grain Zn concentration was negatively correlated with Olsen P. Grain Zn uptake differed little among soils. Application of P to the soils increased grain yield insignificantly and P concentration significantly; however, it reduced grain Zn concentration (particularly at low Olsen P values). Applying Zn alone only increased grain Zn concentration, whereas applying P and Zn in combination increased yield and grain Zn concentration at low and high Olsen P values, respectively. CONCLUSION: Applying P alone to plants grown on calcareous Vertisols low in available P and Zn may in practice reduce grain Zn concentrations while not increasing grain yield significantly. © 2016 Society of Chemical Industry.

The severity of iron chlorosis in sensitive plants is related to soil phosphorus levels.

BACKGROUND: Iron (Fe) deficiency chlorosis, a major nutritional problem in plants growing on calcareous soils, is related to the content and reactivity of soil iron oxides and carbonates. The effects of other soil components, however, need elucidation. In this paper we tested the hypothesis that application of high doses of phosphorus (P) to the soil can aggravate Fe chlorosis. RESULTS: Lupin and sorghum were grown on 24 calcareous soils. Leaf chlorophyll concentration (LCC) in lupin decreased with increasing available P/available Fe ratio in the native soil but LCC in sorghum was unaffected by that ratio. Application of P to the soil resulted in significant reduction of LCC and dry weight in lupin. In sorghum, LCC and dry weight were positively affected by P fertilisation for soils poor in available P whereas the opposite effect was generally observed for the P-rich soils. In another experiment where olive plants were pot-grown on two soils during the 2009­2011 period, P fertilisation affected LCC negatively only in 2009 and 2011 and in the soil that was poorer in iron oxides. CONCLUSION: Application of fertiliser P to Fe chlorosis-inducing soils is likely to aggravate this deficiency. However, this effect depends on the plant and the Fe and P statuses of the soil.