RESUMO
Ochratoxin A (OTA) is a widespread and abundant natural carcinogenic mycotoxin produced by several species of Aspergillus and Penicillium fungi. Due to the ubiquitous presence of these fungi in food and potential risk for human health, a rapid and sensitive in vitro detection assay is required. Analytical methods for OTA detection/identification are generally based on liquid-liquid extraction, clean-up using an immunoaffinity column (IAC), and identification by reversed-phase high pressure liquid chromatography with fluorescence detection (HPLC-FLD). However, IACs are costly and have a short lifespan. Therefore, an interesting approach would appear to be the design and chemical synthesis of a mimotope peptide simulating mycotoxin-specific antibodies. We have developed a promising alternative method that is based on the use of peptides which are able to bind to specific chemical functions and/or molecular structures. Accordingly, a number of peptides (derived from the structures of major redox proteins) were selected and produced by chemical solid phase syntheses. The ability of such peptides to bind to ochratoxin A was evaluated by HPLC. The peptide NF04 (structurally derived from an oxidoreductase enzyme), which was found to be the sole potently reactive compound among tested molecules, was further evaluated in a peptide-based enzyme-linked immunosorbent assay (peptide-based ELISA), thus confirming its specific interaction with ochratoxin A.
Assuntos
Técnicas Biossensoriais/instrumentação , Ensaio de Imunoadsorção Enzimática/instrumentação , Ocratoxinas/análise , Ocratoxinas/química , Peptídeos/análise , Peptídeos/química , Mapeamento de Interação de Proteínas/instrumentação , Desenho de Equipamento , Análise de Falha de Equipamento , Micotoxinas/análise , Micotoxinas/química , Reprodutibilidade dos Testes , Sensibilidade e EspecificidadeRESUMO
Maurotoxin (MTX) is a 34-mer scorpion toxin cross-linked by four disulphide bridges that acts on various K+ channel types. It folds according to an alpha/beta scaffold, i.e., a helix connected to a two stranded beta-sheet by two disulphide bridges. In a former study, various parameters that affect the oxidation and folding of the reduced form of synthetic MTX were investigated in vitro. It was found that MTX achieves its final 3-D structure by evolving over time through a series of oxidation intermediates, from the least to the most oxidized species. MTX oxidative intermediates can be studied by iodoacetamide alkylation of free cysteine residues followed by mass spectrometry analysis. Here, we have analysed the effect of Cu2+ (0.1 to 50 mM) on the kinetics of MTX oxidative folding and found that it dramatically speeds up the formation of the four-disulphide bridged, native-like, MTX (maximal production within 30 minutes instead of > 60 hours). This catalysing effect of Cu2+ was found to be concentration-dependent, reaching a plateau at 10 mM copper ions. Cu2+ was also found to prevent the slow transition of a three disulphide-bridged MTX intermediate towards the final four disulphide-bridged product (12% of total MTX). The data are discussed in light of the potential effects of Cu2+ on MTX secondary structure formation, disulphide bridging and peptidyl prolyl cis-trans isomerization.
Assuntos
Cobre/farmacologia , Dissulfetos/química , Neurotoxinas/química , Dobramento de Proteína , Venenos de Escorpião/química , Venenos de Escorpião/metabolismo , Alquilação , Sequência de Aminoácidos , Animais , Dicroísmo Circular , Técnicas In Vitro , Iodoacetamida/farmacologia , Camundongos , Camundongos Endogâmicos C57BL , Dados de Sequência Molecular , Oxirredução , Estrutura Secundária de Proteína , Venenos de Escorpião/síntese química , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por MatrizRESUMO
The lactococcin B (LnB) is a hydrophobic, positively charged bacteriocin, produced by Lactococcus lactis ssp. cremoris 9B4. It consists of a peptidic chain made up of 47 amino acid residues, and inhibits Lactococcus exclusively. In order to study its biological activity a synthetic lactococcin B (LnBs) was obtained by solid-phase chemical synthesis using a Fmoc strategy. LnBs was shown to be indistinguishable from the natural peptide. In addition, a synthetic (7-47) LnBst analogue was obtained by withdrawal of peptidyl-resin after the 41 cycle of LnBs peptide chain assembly. The synthetic N-terminal truncated (7-47) LnBst analogue was found to be inactive on indicator strains. Our results strongly suggest that the first six N-terminal amino acid residues are involved in the bactericidal activity of LnB.
Assuntos
Bacteriocinas/síntese química , Bacteriocinas/genética , Sequência de Aminoácidos/genética , Aminoácidos/genética , Bacteriocinas/química , Bacteriocinas/isolamento & purificação , Cromatografia Líquida de Alta Pressão , Fluorenos , Interações Hidrofóbicas e Hidrofílicas , Lactococcus lactis/química , Lactococcus lactis/genética , Lactococcus lactis/fisiologia , Dados de Sequência Molecular , Peptídeos/síntese química , Peptídeos/química , Peptídeos/genética , Estrutura Secundária de Proteína/genética , Alinhamento de Sequência , Análise de Sequência de Proteína , Homologia de Sequência de Aminoácidos , Extração em Fase Sólida/métodosRESUMO
It has been shown that A2A adenosine receptors are implicated in pain modulation. The precise mechanism by which activation of A2A receptors produces analgesic effects, however, remains unclear. The aim of this study was to investigate the possible involvement of apamin-sensitive calcium-activated potassium channels (SKCa) and voltage-gated potassium (Kv) channels in A2A receptor activation-induced analgesic effects. Using mice, we evaluated the influence of apamin, a non specific blocker of SKCa channels, Lei-Dab7 (an analog of scorpion Leiurotoxin), a selective blocker of SKCa2 channels, and kaliotoxin (KTX) a Kv channel blocker, on the CGS 21680 (A2A adenosine receptor agonist)-induced increases in hot plate and tail pinch latencies. All drugs were injected in mice via the intracerebroventricular route. We found that apamin and Lei-Dab7, but not KTX, reduced antinociception produced by CGS21680 on the hot plate and tail pinch tests in a dose dependent manner. Lei-Dab 7 was more potent than apamin in this regard. We conclude that SKCa but not Kv channels are implicated in CGS 21680-induced antinociception.
Assuntos
Antagonistas do Receptor A2 de Adenosina , Adenosina/análogos & derivados , Adenosina/farmacologia , Analgésicos/farmacologia , Dor/prevenção & controle , Fenetilaminas/farmacologia , Canais de Potássio Cálcio-Ativados/antagonistas & inibidores , Canais de Potássio de Abertura Dependente da Tensão da Membrana/antagonistas & inibidores , Adenosina/administração & dosagem , Analgésicos/administração & dosagem , Animais , Apamina/farmacologia , Relação Dose-Resposta a Droga , Antagonismo de Drogas , Quimioterapia Combinada , Injeções Intraventriculares , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Medição da Dor , Limiar da Dor/efeitos dos fármacos , Fenetilaminas/administração & dosagem , Venenos de Escorpião/farmacologiaRESUMO
Maurotoxin, a 34-amino acid toxin from Scorpio maurus scorpion venom, was examined for its ability to inhibit cloned human SK (SK1, SK2, and SK3), IK1, and Slo1 calcium-activated potassium (K(Ca)) channels. Maurotoxin was found to produce a potent inhibition of Ca(2+)-activated (86)Rb efflux (IC(50), 1.4 nM) and inwardly rectifying potassium currents (IC(50), 1 nM) in CHO cells stably expressing IK1. In contrast, maurotoxin produced no inhibition of SK1, SK2, and SK3 small-conductance or Slo1 large-conductance K(Ca) channels at up to 1 microM in physiologically relevant ionic strength buffers. Maurotoxin did inhibit (86)Rb efflux (IC(50), 45 nM) through, and (125)I-apamin binding (K(i), 10 nM) to SK channels in low ionic strength buffers (i.e., 18 mM sodium, 250 mM sucrose), which is consistent with previous reports of inhibition of apamin binding to brain synaptosomes. Under similar low ionic strength conditions, the potency for maurotoxin inhibition of IK1 increased by approximately 100-fold (IC(50), 14 pM). In agreement with its ability to inhibit recombinant IK1 potassium channels, maurotoxin was found to potently inhibit the Gardos channel in human red blood cells and to inhibit the K(Ca) in activated human T lymphocytes without affecting the voltage-gated potassium current encoded by Kv1.3. Maurotoxin also did not inhibit Kv1.1 potassium channels but potently blocked Kv1.2 (IC(50), 0.1 nM). Mutation analysis indicates that similar amino acid residues contribute to the blocking activity of both IK1 and Kv1.2. The results from this study show that maurotoxin is a potent inhibitor of the IK1 subclass of K(Ca) potassium channels and may serve as a useful tool for further defining the physiological role of this channel subtype.
Assuntos
Bloqueadores dos Canais de Potássio/farmacologia , Canais de Potássio Cálcio-Ativados , Canais de Potássio/metabolismo , Venenos de Escorpião/farmacologia , Sequência de Aminoácidos , Animais , Apamina/metabolismo , Células CHO , Cricetinae , Humanos , Canais de Potássio Ativados por Cálcio de Condutância Intermediária , Radioisótopos do Iodo , Dados de Sequência Molecular , Homologia de Sequência de Aminoácidos , Linfócitos T/efeitos dos fármacos , Linfócitos T/metabolismo , TransfecçãoRESUMO
A new antimicrobial peptide, referred to as MMFII, was purified to homogeneity from lactic acid bacteria Lactococcus lactis, which were isolated from Tunisian dairy product. The complete amino acid sequence of the peptide has been established by amino acid analysis, Edman sequencing, and mass spectrometry and verified by solid-phase chemical synthesis. MMFII is a single-chain 37-residue polypeptide containing a single intramolecular disulfide bond, i.e., TSYGNGVHCNKSKCWIDVSELETYKAGTVSNPKDILW. It shares ca. 35% sequence identity with Leucocin A, a class IIa bacteriocin. Modeling based on the 3-D of Leucocin A shows three beta strands located in the N-terminal region (Thr1-Tyr3, Val7-Asn10, Lys13-Ile16) and an alpha helical domain from Asp17 to Asn31. When plotted as an alpha-helical wheel, the central alpha-helix of MMFII does not exhibit an amphipathic helical structure. The synthetic MMFII (sMMFII), obtained by the solid-phase method, was shown to be indistinguishable from the natural peptide. sMMFII is active against Lactococcus cremoris and Listeria ivanovii bacteria, whereas no activity was detected for any of the synthetic N-terminal truncated MMFII analogs Cys9-Trp37, Trp15-Trp37, and Val18-Trp37.
Assuntos
Bacteriocinas/química , Bacteriocinas/síntese química , Peptídeos/química , Peptídeos/síntese química , Sequência de Aminoácidos , Bacteriocinas/genética , Bacteriocinas/farmacologia , Microbiologia de Alimentos , Lactococcus/efeitos dos fármacos , Lactococcus lactis/química , Lactococcus lactis/genética , Listeria/efeitos dos fármacos , Modelos Moleculares , Dados de Sequência Molecular , Peptídeos/genética , Peptídeos/farmacologia , Conformação ProteicaRESUMO
Maurotoxin (MTX) is a 34-mer scorpion toxin cross-linked by four disulphide bridges that acts on various K(+) channel subtypes. MTX adopts a disulphide bridge organization of the type C1-C5, C2-C6, C3-C4 and C7-C8, and folds according to the common alpha/beta scaffold reported for other known scorpion toxins. Here we have investigated the process and kinetics of the in vitro oxidation/folding of reduced synthetic L-MTX (L-sMTX, where L-MTX contains only L-amino acid residues). During the oxidation/folding of reduced L-sMTX, the oxidation intermediates were blocked by iodoacetamide alkylation of free cysteine residues, and analysed by MS. The L-sMTX intermediates appeared sequentially over time from the least (intermediates with one disulphide bridge) to the most oxidized species (native-like, four-disulphide-bridged L-sMTX). The mathematical formulation of the diffusion-collision model being inadequate to accurately describe the kinetics of oxidation/folding of L-sMTX, we have formulated a derived mathematical description that better fits the experimental data. Using this mathematical description, we have compared for the first time the oxidation/folding of L-sMTX with that of D-sMTX, its stereoisomer that contains only D-amino acid residues. Several experimental parameters, likely to affect the oxidation/folding process, were studied further; these included temperature, pH, ionic strength, redox potential and concentration of reduced toxin. We also assessed the effects of some cellular enzymes, peptidylprolyl cis-trans isomerase (PPIase) and protein disulphide isomerase (PDI), on the folding pathways of reduced L-sMTX and D-sMTX. All the parameters tested affect the oxidative folding of sMTX, and the kinetics of this process were indistinguishable for L-sMTX and D-sMTX, except when stereospecific enzymes were used. The most efficient conditions were found to be: 50 mM Tris/HCl/1.4 mM EDTA, pH 7.5, supplemented by 0.5 mM PPIase and 50 units/ml PDI for 0.1 mM reduced compound. These data represent the first report of potent stereoselective effects of cellular enzymes on the oxidation/folding of a scorpion toxin.
Assuntos
Dobramento de Proteína , Venenos de Escorpião/química , Venenos de Escorpião/metabolismo , Alquilação , Dissulfetos , Humanos , Indicadores e Reagentes , Iodoacetamida , Cinética , Modelos Teóricos , Neurotoxinas/química , Oxirredução , Peptidilprolil Isomerase/metabolismo , Isomerases de Dissulfetos de Proteínas/metabolismo , Proteínas Recombinantes/metabolismo , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por MatrizRESUMO
Apamin-sensitive small conductance calcium-activated potassium channels (SKCa1-3) mediate the slow afterhyperpolarization in neurons, but the molecular identity of the channel has not been defined because of the lack of specific inhibitors. Here we describe the structure-based design of a selective inhibitor of SKCa2. Leiurotoxin I (Lei) and PO5, peptide toxins that share the RXCQ motif, potently blocked human SKCa2 and SKCa3 but not SKCa1, whereas maurotoxin, Pi1, Tskappa, and PO1 were ineffective. Lei blocked these channels more potently than PO5 because of the presence of Ala(1), Phe(2), and Met(7). By replacing Met(7) in the RXCQ motif of Lei with the shorter, unnatural, positively charged diaminobutanoic acid (Dab), we generated Lei-Dab(7), a selective SKCa2 inhibitor (K(d) = 3.8 nm) that interacts with residues in the external vestibule of the channel. SKCa3 was rendered sensitive to Lei-Dab(7) by replacing His(521) with the corresponding SKCa2 residue (Asn(367)). Intracerebroventricular injection of Lei-Dab(7) into mice resulted in no gross central nervous system toxicity at concentrations that specifically blocked SKCa2 homotetramers. Lei-Dab(7) will be a useful tool to investigate the functional role of SKCa2 in mammalian tissues.
Assuntos
Peptídeos/química , Bloqueadores dos Canais de Potássio , Canais de Potássio Cálcio-Ativados/antagonistas & inibidores , Canais de Potássio/química , Venenos de Escorpião/farmacologia , Alanina/farmacologia , Sequência de Aminoácidos , Animais , Arginina/química , Células COS , Linhagem Celular , Clonagem Molecular , Relação Dose-Resposta a Droga , Eletrofisiologia , Humanos , Cinética , Metionina/farmacologia , Modelos Moleculares , Dados de Sequência Molecular , Mutagênese Sítio-Dirigida , Mutação , Neurotoxinas/farmacologia , Células PC12 , Peptídeos/farmacologia , Fenilalanina/farmacologia , Ligação Proteica , Ratos , Venenos de Escorpião/química , Homologia de Sequência de Aminoácidos , Canais de Potássio Ativados por Cálcio de Condutância Baixa , Treonina/química , Transfecção , Valina/química , Ácido gama-Aminobutírico/farmacologiaRESUMO
The use of acoustics to determine the pore properties of soils, such as porosity, permeability, and tortuosity, is well established. A theoretical surface impedance and complex bulk wavenumber was developed by K. Attenborough for porous media that incorporated the soil pore properties as parameters [J. Acoust. Soc. Am. 73, 785-799 (1983)]. Acoustic level difference measurements were used as a noninvasive means of finding the soil pore properties. Acoustic reflection measurements showed that the sound field over porous rough surfaces is modified by the surface impedance and by surface roughness. It is not possible to separate the signal modification due to impedance and the signal modification from roughness scattering in a forward scattering measurement. In order to accurately determine the soil pore properties, the roughness effects must be known independently from the surface impedance. A means of measuring roughness apart from impedance would allow the effects of roughness to be taken out of the level difference measurements. The underwater acoustics community has used acoustic backscatter for many years to examine surface roughness. The feasibility of adapting these acoustic backscatter techniques to outdoor porous soil surfaces is examined.
RESUMO
Maurotoxin (MTX) is a 34-residue toxin that has been isolated from the venom of the chactidae scorpion Scorpio maurus palmatus, and characterized. Together with Pi1 and HsTx1, MTX belongs to a family of short-chain four-disulfide-bridged scorpion toxins acting on potassium channels. However, contrary to other members of this family, MTX exhibits an uncommon disulfide bridge organization of the type C1-C5, C2-C6, C3-C4 and C7-C8, versus C1-C5, C2-C6, C3-C7 and C4-C8 for both Pi1 and HsTx1. Here, we report that the substitution of MTX proline residues located at positions 12 and/or 20, adjacent to C3 (Cys(13)) and C4 (Cys(19)), results in conventional Pi1- and HsTx1-like arrangement of the half-cystine pairings. In this case, this novel disulfide bridge arrangement is without obvious incidence on the overall three-dimensional structure of the toxin. Pharmacological assays of this structural analog, [A(12),A(20)]MTX, reveal that the blocking activities on Shaker B and rat Kv1.2 channels remain potent whereas the peptide becomes inactive on rat Kv1.3. These data indicate, for the first time, that discrete point mutations in MTX can result in a marked reorganization of the half-cystine pairings, accompanied with a novel pharmacological profile for the analog.
Assuntos
Dissulfetos/química , Canais de Potássio de Abertura Dependente da Tensão da Membrana , Prolina/química , Venenos de Escorpião/química , Sequência de Aminoácidos , Animais , Apamina/metabolismo , Ligação Competitiva , Relação Dose-Resposta a Droga , Feminino , Radioisótopos do Iodo , Canal de Potássio Kv1.2 , Canal de Potássio Kv1.3 , Espectroscopia de Ressonância Magnética , Potenciais da Membrana/efeitos dos fármacos , Dados de Sequência Molecular , Mutação , Oócitos/efeitos dos fármacos , Oócitos/metabolismo , Oócitos/fisiologia , Peptídeos/antagonistas & inibidores , Peptídeos/genética , Peptídeos/fisiologia , Bloqueadores dos Canais de Potássio , Canais de Potássio/genética , Canais de Potássio/fisiologia , Prolina/genética , Conformação Proteica , Ratos , Venenos de Escorpião/metabolismo , Venenos de Escorpião/farmacologia , Análise de Sequência de Proteína , Superfamília Shaker de Canais de Potássio , Sinaptossomos/metabolismo , XenopusRESUMO
Kaliotoxin (KTX), a blocker of voltage-gated potassium channels (Kv), is highly selective for Kv1.1 and Kv1.3. First, Kv1.3 is expressed by T lymphocytes. Blockers of Kv1.3 inhibit T lymphocyte activation. Second, Kv1.1 is found in paranodal regions of axons in the central nervous system. Kv blockers improve the impaired neuronal conduction of demyelinated axons in vitro and potentiate the synaptic transmission. Therefore, we investigated the therapeutic properties of KTX via its immunosuppressive and symptomatic neurological effects, using experimental autoimmune encephalomyelitis (EAE), an animal model for multiple sclerosis. The T line cells used to induce adoptive EAE were myelin basic protein (MBP)-specific, constitutively contained mRNA for Kv1.3. and expressed Kv1.3. These channels were shown to be blocked by KTX. Activation is a crucial step for MBP T cells to become encephalitogenic. The addition of KTX during Ag-T cell activation led to a great reduction in the MBP T cell proliferative response, in the production of IL-2 and TNF, and in Ca(2+) influx. Furthermore, the addition of KTX during T cell activation in vitro led a decreased encephalitogenicity of MBP T cells. Moreover, KTX injected into Lewis rats impaired T cell function such as the delayed-type hypersensitivity. Lastly, the administration of this blocker of neuronal and lymphocyte channels to Lewis rats improved the symptoms of EAE. We conclude that KTX is a potent immunosuppressive agent with beneficial effects on the neurological symptoms of EAE.
Assuntos
Encefalomielite Autoimune Experimental/imunologia , Encefalomielite Autoimune Experimental/prevenção & controle , Imunossupressores/farmacologia , Ativação do Canal Iônico/efeitos dos fármacos , Ativação Linfocitária/efeitos dos fármacos , Bloqueadores dos Canais de Potássio , Canais de Potássio de Abertura Dependente da Tensão da Membrana , Linfócitos T/efeitos dos fármacos , Transferência Adotiva , Animais , Antígenos/farmacologia , Cálcio/antagonistas & inibidores , Cálcio/metabolismo , Linhagem Celular , Encefalomielite Autoimune Experimental/tratamento farmacológico , Epitopos de Linfócito T/imunologia , Feminino , Cobaias , Humanos , Hipersensibilidade Tardia/imunologia , Hipersensibilidade Tardia/prevenção & controle , Injeções Subcutâneas , Interleucina-2/antagonistas & inibidores , Interleucina-2/biossíntese , Líquido Intracelular/efeitos dos fármacos , Líquido Intracelular/metabolismo , Ativação do Canal Iônico/imunologia , Células Jurkat , Canal de Potássio Kv1.3 , Ativação Linfocitária/imunologia , Camundongos , Proteína Básica da Mielina/antagonistas & inibidores , Proteína Básica da Mielina/imunologia , Proteína Básica da Mielina/farmacologia , Técnicas de Patch-Clamp , Canais de Potássio/biossíntese , Canais de Potássio/genética , RNA Mensageiro/biossíntese , Ratos , Ratos Endogâmicos Lew , Venenos de Escorpião/administração & dosagem , Venenos de Escorpião/farmacologia , Linfócitos T/imunologia , Linfócitos T/metabolismo , Linfócitos T/transplante , Fator de Necrose Tumoral alfa/antagonistas & inibidores , Fator de Necrose Tumoral alfa/biossínteseRESUMO
The auxiliary beta subunit importantly regulates voltage-dependent Ca(2+) channel activity through an interaction with the AID domain, a binding site located in the cytoplasmic I-II linker of the ion-conducting alpha(1) subunit. In the present study, we used two synthetic peptides corresponding to partial sequences of the I-II linker of alpha(1A) (AID(A)-peptides) as tools to disrupt the alpha(1)-beta interaction. In vitro binding experiments confirmed that these peptides exhibit a reasonable affinity to the neuronal beta(3) subunit to serve this purpose, although they failed to prevent immunoprecipitation of native N- and P/Q-type channels by anti-beta(3) antibodies. Together, our results (i) provide evidence for the reversibility of channel subunit association suggesting that the disruption of the alpha(1)-beta interaction may be a possible mechanism for Ca(2+) channel regulation in vivo, and (ii) support a model whereby the alpha(1)-beta association is based on multiple interaction sites.
Assuntos
Canais de Cálcio/metabolismo , Sequência de Aminoácidos , Animais , Sítios de Ligação , Dados de Sequência Molecular , Conformação Proteica , Coelhos , RatosRESUMO
Maurotoxin (MTX) is a scorpion toxin acting on several K(+) channel subtypes. It is a 34-residue peptide cross-linked by four disulfide bridges that are in an "uncommon" arrangement of the type C1-C5, C2-C6, C3-C4, and C7-C8 (versus C1-C5, C2-C6, C3-C7, and C4-C8 for Pi1 or HsTx1, two MTX-related scorpion toxins). We report here that a single mutation in MTX, in either position 15 or 33, resulted in a shift from the MTX toward the Pi1/HsTx1 disulfide bridge pattern. This shift is accompanied by structural and pharmacological changes of the peptide without altering the general alpha/beta scaffold of scorpion toxins.
Assuntos
Dissulfetos , Venenos de Escorpião/química , Sequência de Aminoácidos , Animais , Encéfalo/metabolismo , Cromatografia Líquida de Alta Pressão , Cisteína/química , Relação Dose-Resposta a Droga , Eletrofisiologia , Cinética , Dose Letal Mediana , Ligantes , Espectroscopia de Ressonância Magnética , Camundongos , Camundongos Endogâmicos C57BL , Modelos Moleculares , Dados de Sequência Molecular , Mutação , Oócitos/metabolismo , Biossíntese Peptídica , Mutação Puntual , Canais de Potássio/química , Conformação Proteica , Estrutura Secundária de Proteína , Ratos , Venenos de Escorpião/genética , Homologia de Sequência de Aminoácidos , Sinaptossomos/metabolismo , Fatores de Tempo , XenopusRESUMO
Pi1 is a 35-residue toxin cross-linked by four disulfide bridges that has been isolated from the venom of the chactidae scorpion Pandinus imperator. Due to its very low abundance in the venom, we have chemically synthesized this toxin in order to study its biological activity. Enzyme-based proteolytic cleavage of the synthetic Pi1 (sPi1) demonstrates half-cystine pairings between Cys4-Cys25, Cys10-Cys30, Cys14-Cys32 and Cys20-Cys35, which is in agreement with the disulfide bridge organization initially reported on the natural toxin. In vivo, intracerebroventricular injection of sPi1 in mice produces lethal effects with an LD50 of 0.2 microgram per mouse. In vitro, the application of sPi1 induces drastic inhibition of Shaker B (IC50 of 23 nM) and rat Kv1.2 channels (IC50 of 0.44 nM) heterologously expressed in Xenopus laevis oocytes. No effect was observed on rat Kv1.1 and Kv1.3 currents upon synthetic peptide application. Also, sPi1 is able to compete with 125I-labeled apamin for binding onto rat brain synaptosomes with an IC50 of 55 pM. Overall, these results demonstrate that sPi1 displays a large spectrum of activities by blocking both SK- and Kv1-types of K+ channels; a selectivity reminiscent of that of maurotoxin, another structurally related four disulfide-bridged scorpion toxin that exhibits a different half-cystine pairing pattern.
Assuntos
Bloqueadores dos Canais de Potássio , Canais de Potássio de Abertura Dependente da Tensão da Membrana , Venenos de Escorpião/síntese química , Venenos de Escorpião/farmacologia , Sequência de Aminoácidos , Animais , Ventrículos Cerebrais/efeitos dos fármacos , Ventrículos Cerebrais/fisiologia , Cistina , Dissulfetos/análise , Humanos , Injeções Intraventriculares , Canal de Potássio Kv1.1 , Canal de Potássio Kv1.2 , Canal de Potássio Kv1.3 , Dose Letal Mediana , Camundongos , Dados de Sequência Molecular , Canais de Potássio/fisiologia , Ratos , Venenos de Escorpião/química , Alinhamento de Sequência , Homologia de Sequência de Aminoácidos , Xenopus laevisRESUMO
Maurotoxin (alpha-KTx6.2) is a toxin derived from the Tunisian chactoid scorpion Scorpio maurus palmatus, and it is a member of a new family of toxins that contain four disulfide bridges (, Eur. J. Biochem. 254:468-479). We investigated the mechanism of the maurotoxin action on voltage-gated K(+) channels expressed in Xenopus oocytes. Maurotoxin blocks the channels in a voltage-dependent manner, with its efficacy increasing with greater hyperpolarization. We show that an amino acid residue in the external mouth of the channel pore segment that is known to be involved in the actions of other peptide toxins is also involved in maurotoxin's interaction with the channel. We conclude that, despite the unusual disulfide bridge pattern, the mechanism of the maurotoxin action is similar to those of other K(+) channel toxins with only three disulfide bridges.
Assuntos
Canais de Potássio/fisiologia , Venenos de Escorpião/farmacologia , Sequência de Aminoácidos , Substituição de Aminoácidos , Animais , Charibdotoxina/química , Charibdotoxina/farmacologia , Feminino , Ativação do Canal Iônico/efeitos dos fármacos , Cadeias de Markov , Potenciais da Membrana/efeitos dos fármacos , Dados de Sequência Molecular , Mutagênese Sítio-Dirigida , Neurotoxinas/farmacologia , Oócitos/fisiologia , Técnicas de Patch-Clamp , Bloqueadores dos Canais de Potássio , Canais de Potássio/química , Conformação Proteica , Proteínas Recombinantes/antagonistas & inibidores , Proteínas Recombinantes/química , Venenos de Escorpião/química , Alinhamento de Sequência , Deleção de Sequência , Superfamília Shaker de Canais de Potássio , Xenopus laevisRESUMO
Maurotoxin is a 34-residue toxin isolated from the venom of the Tunisian chactoid scorpion Scorpio maurus palmatus and contains four disulfide bridges that are normally found in long-chain toxins of 60-70 amino acid residues, which affect voltage-gated sodium channels. However, despite the unconventional disulfide-bridge pattern of maurotoxin, the conformation of this toxin remains similar to that of other toxins acting on potassium channels. Here, we analyzed the effects of synthetic maurotoxin on voltage-gated Shaker potassium channels (ShB) expressed in Xenopus oocytes. Maurotoxin produces a strong, but reversible, inhibition of the ShB K+ current with an IC50 of 2 nM. Increasing concentrations of the toxin induce a progressively higher block at saturating concentrations. At nonsaturating concentrations of the toxin (5-20 nM), the channel block appears slightly more pronounced at threshold potentials suggesting that the toxin may have a higher affinity for the closed state of the channel. At the single channel level, the toxin does not modify the unitary current amplitude, but decreases ensemble currents by increasing the number of depolarizing epochs that failed to elicit any opening. A point mutation of Lys23 to alanine in maurotoxin produces a 1000-fold reduction in the IC50 of block by the toxin suggesting the importance of this charged residue for the interaction with the channel. Maurotoxin does not affect K+ currents carried by Kir2.3 channels in oocytes or Na+ currents carried by the alphaIIa channel expressed in CHO cells.
Assuntos
Bloqueadores dos Canais de Potássio , Canais de Potássio/metabolismo , Venenos de Escorpião/farmacologia , Motivos de Aminoácidos , Sequência de Aminoácidos , Animais , Células CHO , Cricetinae , Dissulfetos , Relação Dose-Resposta a Droga , Expressão Gênica , Microinjeções , Dados de Sequência Molecular , Oócitos , Técnicas de Patch-Clamp , Conformação Proteica , RNA Complementar/metabolismo , Venenos de Escorpião/química , Venenos de Escorpião/metabolismo , Superfamília Shaker de Canais de Potássio , Canais de Sódio/metabolismo , Xenopus laevisRESUMO
We determined the structure in solution by (1)H two-dimensional NMR of Maurocalcine from the venom of Scorpio maurus. This toxin has been demonstrated to be a potent effector of ryanodyne-sensitive calcium channel from skeletal muscles. This is the first description of a scorpion toxin which folds following the Inhibitor Cystine Knot fold (ICK) already described for numerous toxic and inhibitory peptides, as well as for various protease inhibitors. Its three dimensional structure consists of a compact disulfide-bonded core from which emerge loops and the N-terminus. A double-stranded antiparallel beta-sheet comprises residues 20-23 and 30-33. A third extended strand (residues 9-11) is perpendicular to the beta-sheet. Maurocalcine structure mimics the activating segment of the dihydropyridine receptor II-III loop and is therefore potentially useful for dihydropyridine receptor/ryanodine receptor interaction studies. Proteins 2000;40:436-442.
Assuntos
Agonistas dos Canais de Cálcio/química , Venenos de Escorpião/química , Sequência de Aminoácidos , Agonistas dos Canais de Cálcio/toxicidade , Canais de Cálcio Tipo L/metabolismo , Simulação por Computador , Modelos Moleculares , Mimetismo Molecular , Dados de Sequência Molecular , Ressonância Magnética Nuclear Biomolecular , Canal de Liberação de Cálcio do Receptor de Rianodina/metabolismo , Venenos de Escorpião/toxicidade , Homologia de Sequência de AminoácidosRESUMO
Maurotoxin (MTX) is a 34-residue toxin that has been isolated from the venom of the chactidae scorpion Scorpio maurus palmatus. The toxin displays an exceptionally wide range of pharmacological activity since it binds onto small conductance Ca(2+)-activated K(+) channels and also blocks Kv channels (Shaker, Kv1.2 and Kv1.3). MTX possesses 53-68% sequence identity with HsTx1 and Pi1, two other K(+) channel short chain scorpion toxins cross-linked by four disulfide bridges. These three toxins differ from other K(+)/Cl(-)/Na(+) channel scorpion toxins cross-linked by either three or four disulfide bridges by the presence of an extra half-cystine residue in the middle of a consensus sequence generally associated with the formation of an alpha/beta scaffold (an alpha-helix connected to an antiparallel beta-sheet by two disulfide bridges). Because MTX exhibits an uncommon disulfide bridge organization among known scorpion toxins (C1-C5, C2-C6, C3-C4, and C7-C8 instead of C1-C4, C2-C5, and C3-C6 for three-disulfide-bridged toxins or C1-C5, C2-C6, C3-C7, and C4-C8 for four-disulfide-bridged toxins), we designed and chemically synthesized an MTX analog with three instead of four disulfide bridges ([Abu(19),Abu(34)]MTX) and in which the entire consensus motif of scorpion toxins was restored by the substitution of the two half-cystines in positions 19 and 34 (corresponding to C4 and C8) by two isosteric alpha-aminobutyrate (Abu) derivatives. The three-dimensional structure of [Abu(19), Abu(34)]MTX in solution was solved by (1)H NMR. This analog adopts the alpha/beta scaffold with now conventional half-cystine pairings connecting C1-C5, C2-C6, and C3-C7 (with C4 and C8 replaced by Abu derivatives). This novel arrangement in half-cystine pairings that concerns the last disulfide bridge results mainly in a reorientation of the alpha-helix regarding the beta-sheet structure. In vivo, [Abu(19),Abu(34)]MTX remains lethal in mice as assessed by intracerebroventricular injection of the peptide (LD(50) value of 0. 25 microg/mouse). The structural variations are also accompanied by changes in the pharmacological selectivity of the peptide, suggesting that the organization pattern of disulfide bridges should affect the three-dimensional presentation of certain key residues critical to the blockage of K(+) channel subtypes.
Assuntos
Desenho de Fármacos , Venenos de Escorpião/química , Toxinas Biológicas/química , Sequência de Aminoácidos , Animais , Dissulfetos , Espectroscopia de Ressonância Magnética , Camundongos , Dados de Sequência Molecular , Conformação Proteica , Venenos de Escorpião/genética , Escorpiões , Toxinas Biológicas/síntese química , Toxinas Biológicas/genéticaRESUMO
Maurocalcine is a novel toxin isolated from the venom of the chactid scorpion Scorpio maurus palmatus. It is a 33-mer basic peptide cross-linked by three disulfide bridges, which shares 82% sequence identity with imperatoxin A, a scorpion toxin from the venom of Pandinus imperator. Maurocalcine is peculiar in terms of structural properties since it does not possess any consensus motif reported so far in other scorpion toxins. Due to its low concentration in venom (0.5% of the proteins), maurocalcine was chemically synthesized by means of an optimized solid-phase method, and purified after folding/oxidation by using both C18 reversed-phase and ion exchange high-pressure liquid chromatographies. The synthetic product (sMCa) was characterized. The half-cystine pairing pattern of sMCa was identified by enzyme-based cleavage and Edman sequencing. The pairings were Cys3-Cys17, Cys10-Cys21, and Cys16-Cys32. In vivo, the sMCa was lethal to mice following intracerebroventricular inoculation (LD(50), 20 microg/mouse). In vitro, electrophysiological experiments based on recordings of single channels incorporated into planar lipid bilayers showed that sMCa potently and reversibly modifies channel gating behavior of the type 1 ryanodine receptor by inducing prominent subconductance behavior.
Assuntos
Canal de Liberação de Cálcio do Receptor de Rianodina/química , Venenos de Escorpião/síntese química , Sequência de Aminoácidos , Animais , Cromatografia Líquida de Alta Pressão , Cistina/química , Eletrofisiologia , Injeções Intraventriculares , Ativação do Canal Iônico , Dose Letal Mediana , Bicamadas Lipídicas , Camundongos , Dados de Sequência Molecular , Venenos de Escorpião/química , Venenos de Escorpião/toxicidade , Análise de Sequência de Proteína , Homologia de Sequência de AminoácidosRESUMO
Maurotoxin (MTX) is a 34-amino acid polypeptide cross-linked by four disulfide bridges that has been isolated from the venom of the scorpion Scorpio maurus palmatus and characterized. Maurotoxin competed with radiolabeled apamin and kaliotoxin for binding to rat brain synaptosomes and blocked K+ currents from Kv1 channel subtypes expressed in Xenopus oocytes. Structural characterization of the synthetic toxin identified half-cystine pairings at Cys3-Cys24, Cys9-Cys29, Cys13-Cys19 and Cys31-Cys34 This disulfide bridge pattern is unique among known scorpion toxins, particularly the existence of a C-terminal '14-membered disulfide ring' (i.e. cyclic domain 31-34), We therefore studied structure-activity relationships by investigating the structure and pharmacological properties of synthetic MTX peptides either modified at the C-terminus ¿i.e. MTX(1-29), [Abu31,34]-MTX and [Cys31,34, Tyr32]D-MTX) or mimicking the cyclic C-terminal domain [i.e. MTX(31-34)]. Unexpectedly, the absence of a disulfide bridge Cys31-Cys34 in [Abu 31,34]-MTX and MTX(1-29) resulted in MTX-unrelated half-cystine pairings of the three remaining disulfide bridges for the two analogs, which is likely to be responsible for their inactivity against Kv1 channel subtypes. Cyclic MTX(31-34) was also biologically inactive. [Cys31,34, Tyr32]D-MTX, which had a 'native', MTX-related, disulfide bridge organization, but a D-residue-induced reorientation of the C-terminal disulfide bridge, was potent at blocking the Kv1.1 channel. This peptide-induced Kv1.1 blockage was voltage-dependent (a property not observed for MTX), maximal in the low depolarization range and associated with on-rate changes in ligand binding. Thus, the cyclic C-terminal domain of MTX seems to be crucial for recognition of Kv1.3, and to a lesser extent, Kv1.2 channels and it may contribute to the stabilization and strength of the interaction between the toxin and the Kv1.1 channel.