Effect of Microsphere Concentration on Catechin Release from Microneedle Arrays.

In this work, microspheres were developed by cross-linking glutaraldehyde in an aqueous gelatin solution with a surfactant and solvent. A poly(vinyl alcohol) (PVA) solution was produced and combined with catechin-loaded microspheres. Different microsphere concentrations (0%, 5%, 10%, and 15%) were applied to the PVA microneedles. The moisture content, particle size, swelling, and drug release percentage of microneedles were studied using various microsphere concentrations. Fourier transform infrared and scanning electron microscopy (SEM) investigations validated the structure of gelatin microspheres as well as their decoration in microneedles. The SEM scans revealed that spherical microspheres with a wrinkled and folded morphology were created, with no physical holes visible on the surface. The gelatin microspheres generated had a mean particle size of 20-30 µm. Ex vivo release analysis indicated that microneedles containing 10% microspheres released the most catechin, with 42.9% at 12 h and 84.4% at 24 h.

Graphene Oxide Nanostructures as Nanoplatforms for Delivering Natural Therapeutic Agents: Applications in Cancer Treatment, Bacterial Infections, and Bone Regeneration Medicine.

Graphene, fullerenes, diamond, carbon nanotubes, and carbon dots are just a few of the carbon-based nanomaterials that have gained enormous popularity in a variety of scientific disciplines and industrial uses. As a two-dimensional material in the creation of therapeutic delivery systems for many illnesses, nanosized graphene oxide (NGO) is now garnering a large amount of attention among these materials. In addition to other benefits, NGO functions as a drug nanocarrier with remarkable biocompatibility, high pharmaceutical loading capacity, controlled drug release capability, biological imaging efficiency, multifunctional nanoplatform properties, and the power to increase the therapeutic efficacy of loaded agents. Thus, NGO is a perfect nanoplatform for the development of drug delivery systems (DDSs) to both detect and treat a variety of ailments. This review article's main focus is on investigating surface functionality, drug-loading methods, and drug release patterns designed particularly for smart delivery systems. The paper also examines the relevance of using NGOs to build DDSs and considers prospective uses in the treatment of diseases including cancer, infection by bacteria, and bone regeneration medicine. These factors cover the use of naturally occurring medicinal substances produced from plant-based sources.

Enhancing Rheological and Textural Properties of Gelatin-Based Composite Gels through Incorporation of Sesame Seed Oleosome-Protein Fillers.

In this study, the protein and oleosomes of sesame seeds were extracted individually and used to prepare a gel composed of gelatin, protein, and oleosomes. Mixtures of gelatin and sesame seeds protein were prepared, and oleosomes with different percentages (0, 10, 20 and 30% of their weight) were used. Different amounts of oleosomes in the composite gel samples were examined for their morphological, rheological, and textural properties. The results of the viscoelastic properties of different composite gel samples indicated that a higher percentage of oleosomes would increase the storage modulus (G'), loss modulus (G″), and complex viscosity (η*). The storage modulus of all gel samples was greater than the loss modulus, suggesting a solid behavior. So, in the sample with 30% oleosome, the storage modulus and the loss modulus reached 143,440 Pascals and 44,530 Pascals. The hardness and breaking force in samples containing 30% oleosome reached 1.29 ± 0.02 and 0.17 ± 0.02, respectively. In general, it can be said that composite gels based on gelatin-sesame seed protein modified with oleosome can be used as a part of food components in various dairy products, gelatin desserts, lean meat products and the production of useful products.

Ex Vivo Transdermal Delivery of Nicotinamide Mononucleotide Using Polyvinyl Alcohol Microneedles.

Nicotinamide mononucleotide (NMN), which has recently been spotlighted as an anti-aging agent, is a precursor of the coenzyme nicotinamide adenine dinucleotide that plays an important role in intracellular redox reactions. NMN capsules for oral administration currently on the market have a problem in that they are almost fully metabolized in the stomach and liver and excreted as nicotinamide. Therefore, there is a need to develop a patient-friendly delivery method that can improve the bioavailability of NMN. For this purpose, various polyvinyl alcohol (PVA)-based microneedle patches were fabricated to develop a transdermal delivery system for NMN. First, the molecular weight effect of PVA on the shape and microstructure of microneedles was studied. After selecting the optimal molecular weight PVA, the swelling of the microneedles and the ex vivo release of NMN were studied. The effect of carboxymethyl cellulose (CMC) and dimethyl sulfoxide on NMN release was also investigated. The highest NMN release of 91.94% in 18 h was obtained using a 9.5 kDa molecular weight PVA microneedle containing NMN and CMC.

Synthesis and Characterization of 𝒌-Carrageenan/PVA Nanocomposite Hydrogels in Combination with MgZnO Nanoparticles to Evaluate the Catechin Release.

In the current study, nanocomposites were prepared by combining k-carrageenan, polyvinyl alcohol (PVA), and doped nanoparticles (Magnesium oxide) MgO, (Magnesium Zinc oxide) MgZnO 1%, MgZnO 3%, and MgZnO 5%. The nanoparticles were synthesized by a sol-gel method and mixed with a mixture of k-carrageenan/PVA (Ca/PVA) in various ratios. The structure of the composites was analyzed using thermogravimetric analysis (TGA), X-ray diffraction (XRD), and Fourier transform infrared spectroscopy (FTIR). The Ca/PVA mixture was then mixed with nanoparticles and loaded with active ingredient, catechin. Scanning electron microscope (SEM) and texture analysis were performed to analyze the nanocomposites. Entrapment efficiency (EE%) and drug release studies confirmed that k-carrageenan/PVA/MgZnO 5% had the highest EE% at 81.58% and a drug release of 75.21% ± 0.94. The EE% of k-carrageenan/PVA/MgO was 55.21% and its drug release was 45%. This indicates that ZnO plays an effective role in the structure and performance of Ca/PVA composites. The SEM images of MgO composites show smoother surfaces compared to MgZnO composites. This may be one of the reasons for the increased EE% and drug release of MgZnO composites. The addition of ZnO to the composite structure can lead to the appearance of pores on the surface of the composite, increasing entrapment and drug release.

Liposome System for Encapsulation of Spirulina platensis Protein Hydrolysates: Controlled-Release in Simulated Gastrointestinal Conditions, Structural and Functional Properties.

This study aimed to evaluate the physicochemical, structural, antioxidant and antibacterial properties of chitosan-coated (0.5 and 1% CH) nanoliposomes containing hydrolyzed protein of Spirulina platensis and its stability in simulated gastric and intestine fluids. The chitosan coating of nanoliposomes containing Spirulina platensis hydrolyzed proteins increased their size and zeta potential. The fourier transform infrared spectroscopy (FT-IR) test showed an effective interaction between the hydrolyzed protein, the nanoliposome, and the chitosan coating. Increasing the concentration of hydrolyzed protein and the percentage of chitosan coating neutralized the decreasing effect of microencapsulation on the antioxidant activity of peptides. Chitosan coating (1%) resulted in improved stability of size, zeta potential, and poly dispersity index (PDI) of nanoliposomes, and lowered the release of the hydrolyzed Spirulina platensis protein from nanoliposomes. Increasing the percentage of chitosan coating neutralized the decrease in antibacterial properties of nanoliposomes containing hydrolyzed proteins. This study showed that 1% chitosan-coated nanoliposomes can protect Spirulina platensis hydrolyzed proteins and maintain their antioxidant and antibacterial activities.

Green Facile Synthesis of Silver-Doped Zinc Oxide Nanoparticles and Evaluation of Their Effect on Drug Release.

Silver doped zinc oxide nanoparticles (ZANPs) were synthesized by the gelatin mediated and polymerized sol-gel method, and a calcination temperature of 700 °C was applied for 2 h. X-ray diffraction (XRD), FESEM, TGA, DSC, and EDS were performed to study the structure of the prepared nano-powders. Both cubic silver and hexagonal ZnO diffraction peaks were detected in the XRD patterns. The XRD results, analyzed by the size strain plot (SSP) and Scherrer methods, showed that the crystalline sizes of these nanoparticles increased as the Ag concentration increased. The results were observed via transition electron microscopy (TEM), where the particle size of the prepared samples was increased in the presence of silver. Catechin was chosen as a drug model and was loaded into the hydrogels for release studies. The drug content percentage of catechin in the hydrogels showed a high loading of the drug, and the highest rate was 98.59 ± 2.11%, which was attributed to the Zn0.97Ag0.03O hydrogels. The swelling of the samples and in vitro release studies were performed. The results showed that Zn0.91Ag0.09O showed the highest swelling ratio (68 ± 3.40%) and, consequently, the highest release (84 ± 2.18%) within 300 min. The higher amount of silver ions in the hydrogel structure causes it to enhance the osmotic pressure of the inner structure and increases the relaxation of the structure chain.

Microneedles for transdermal drug delivery using clay-based composites.

INTRODUCTION: Clays provide a powerful drug delivery system due to their specific structure, which can interact with drugs and control their release. Transdermal delivery avoids the transformation of a drug in the liver by dispensing the drug through the skin, avoiding the gastrointestinal tract. Due to ion exchange and the multilayer structure of clay sheets, drugs are entrapped between the sheets, showing this material's high drug release capacity. Recent studies have shown that the addition of 5 wt% clay is suitable for transdermal delivery applications. AREAS COVERED: In this review, we discussed clay-based polymer microneedles, mechanisms of clay-drug interactions, and antimicrobial effects of clays. We also investigated carriers for clay transdermal drug delivery and in vitro drug release from clay-drug composites. In addition, the most important clays, montmorillonite, halloysite, and sepiolite, were compared and discussed. EXPERT OPINION: The manufactured product represents an interesting method of sustained drug release through the skin. The duration of drug release can be tuned by modifying the polymer concentration and an appropriate molecular weight in the polymer system. When using clay as a non-polymer transdermal drug carrier, this adjustment is possible by changing the type and concentration of clay.

Recent progress in polymeric non-invasive insulin delivery.

The design of carriers for insulin delivery has recently attracted major research attentions in the biomedical field. In general, the release of drug from polymers is driven via a variety of polymers. Several mechanisms such as matrix release, leaching of drug, swelling, and diffusion are usually adopted for the release of drug through polymers. Insulin is one of the most predominant therapeutic drugs for the treatment of both diabetes mellitus; type-I (insulin-dependent) and type II (insulin-independent). Currently, insulin is administered subcutaneously, which makes the patient feel discomfort, pain, hyperinsulinemia, allergic responses, lipodystrophy surrounding the injection area, and occurrence of miscarried glycemic control. Therefore, significant research interest has been focused on designing and developing new insulin delivery technologies to control blood glucose levels and time, which can enhance the patient compliance simultaneously through alternative routes as non-invasive insulin delivery. The aim of this review is to emphasize various non-invasive insulin delivery mechanisms including oral, transdermal, rectal, vaginal, ocular, and nasal. In addition, this review highlights different smart stimuli-responsive insulin delivery systems including glucose, pH, enzymes, near-infrared, ultrasound, magnetic and electric fields, and the application of various polymers as insulin carriers. Finally, the advantages, limitations, and the effect of each non-invasive route on insulin delivery are discussed in detail.

Recent advances in polymeric transdermal drug delivery systems.

Transdermal delivery has proven to be one of the most favorable methods among novel drug delivery systems. Since drugs administered by transdermal delivery systems avoid the gastrointestinal tract, and thus avoid conversion by the liver, the likelihood of liver dysfunction and gastrointestinal tract irritation as side effects is low. Drug delivery through the skin has other advantages, such as maintaining an effective rate of drug delivery over time, a steady rate of circulation, and the benefits of a passive delivery system and diffusion. Transdermal drug delivery is achieved using patches which consist of different and specific layers. In the last few decades, many types of patches have been approved worldwide, such as medical plasters, which have been generally applied to the skin for localized diseases. Such patches can be traced back to ancient China (around 2000 BCE) and are the early precursors of today's transdermal patches. With the help of effective design, materials, manufacturing, and evaluation, a large number of drugs can now be administered using this valuable advanced technology. This study reviews different types of polymer patches, their advantages and disadvantages, and different studies related to transdermal drug delivery methods, and the advantages and disadvantages of each method. Different mechanisms of transdermal drug delivery system with patches are also discussed.

Development of curcumin-loaded Prunus armeniaca gum nanoparticles: Synthesis, characterization, control release behavior, and evaluation of anticancer and antimicrobial properties.

The present work was conducted to develop a new polysaccharide-based encapsulation system via electrostatic interactions between Prunus armeniaca gum exudates (PAGE) and Ca2+ ions to enhance the biological activity and bioavailability of curcumin. The effects of different levels of pH (6, 7, and 8) and ion concentrations (1, 3, and 5) on the particle diameter and surface charge of the samples were examined. The encapsulation efficiency in the PAGE-based nanoparticles was realized to be 86.1%, indicating the encapsulation technique applied in this study was effective to entrap most of the curcumin within the PAGE matrix. The nanoparticles showed a smooth surface with spherical shape. Fourier transform infrared spectroscopy (FT-IR) and X-ray diffraction (X-ray) studies confirmed the formation of polyelectrolyte complexation. The cumulative release of curcumin in simulated gastrointestinal tract was less than 75%, revealing a gradual release trend. Both pure curcumin and curcumin-loaded nanoparticles were toxic to the cancer cell lines.

Green Synthesis of Mg0.99 Zn0.01O Nanoparticles for the Fabrication of κ-Carrageenan/NaCMC Hydrogel in order to Deliver Catechin.

Currently, the role of the nanoparticles in the structure of the composites and their benefits for the health of the body is valuable. In this study, the effects of the doping on the structural and morphological properties of the hydrogels using a Mg co-doped ZnO hydrogel, which has been fabricated by the sol-gel process, have been investigated. Then, a hydrogel containing nanoparticle and a hydrogel without any nanoparticles was produced as a control. The hydrogels were loaded with catechin and the related characterization was evolved based on the new structure of the matrices. The Mg0.99Zn0.01O nanoparticles were synthesized using a green synthesis method. To investigate the properties of the nanoparticles, zeta potential and XRD were studied. The field emission scanning electron microscopy (FESEM), FTIR, TGA, swelling Ratio, and compression tests were investigated for the hydrogels. Based on the results, FESEM showed a more compressed structure for hydrogels including nanoparticles rather than the hydrogels without a nanoparticle. The TGA showed a higher decomposition temperature in the hydrogels including nanoparticles. The swelling ratio of hydrogels containing a nanoparticle was higher than the control hydrogel. κ-Carrageenan/ Mg0.99Zn0.01O/NaCMC/Catechin had the highest swelling ratio (44.15%) rather than the κ-Carrageenan/NaCMC (33.22%). Mg0.99Zn0.01O nanoparticles presented a stronger structure of hydrogels in the compression test. It is concluded that the role of the synthesized nanoparticle is critical in the structure of the hydrogel.

From formulation of acrylamide-based hydrogels to their optimization for drug release using response surface methodology.

This study conducted on the structure of modified acrylamide-based hydrogel by synthesizing the nano composites. The hydrogels employed in this study were provided through a combination of acrylamide monomers, sodium carboxymethyl cellulose (NaCMC) and magnesium oxide (MgO) nanoparticles by crosslinking polymerization. N,N,N',N'-tetramethylethylenediamine and ammonium persulfate as the initiator was applied in the structure of the polymer. Findings of the study considered the nano composites consisting of MgO have the highest swelling ratio compared to pure Aam hydrogels. Thus, MgO is an appropriate nanoparticle to be used in the nano composites. Response surface methodology (RSM) based on a central composite design (CCD Design) was applied to optimize the preparation variables of a hydrogel consisted of MgO, NaCMC. With the swelling ratio for acrylamide-based hydrogel as the response, the effects of two variables, i.e. MgO and NaCMC were investigated. The effects of pH, temperature, MgO, and NaCMC on the drug release were investigated using the CCD design. The predicted appropriate drug release conditions for the hydrogel at the highest rate of temperature (37.50 °C) and pH: 4.10, is at its highest value, while the lower drug release is at temperature 38 °C and pH 3.50. With the desired value of MgO (0.01 g) and amount of NaCMC (0.1 g).

Perspectives of phytoremediation using water hyacinth for removal of heavy metals, organic and inorganic pollutants in wastewater.

The development of eco-friendly and efficient technologies for treating wastewater is one of the attractive research area. Phytoremediation is considered to be a possible method for the removal of pollutants present in wastewater and recognized as a better green remediation technology. Nowadays the focus is to look for a sustainable approach in developing wastewater treatment capability. Water hyacinth is one of the ancient technology that has been still used in the modern era. Although, many papers in relation to wastewater treatment using water hyacinth have been published, recently removal of organic, inorganic and heavy metal have not been reviewed extensively. The main objective of this paper is to review the possibility of using water hyacinth for the removal of pollutants present in different types of wastewater. Water hyacinth is although reported to be as one of the most problematic plants worldwide due to its uncontrollable growth in water bodies but its quest for nutrient absorption has provided way for its usage in phytoremediation, along with the combination of herbicidal control, integratated biological control and watershed management controlling nutrient supply to control its growth. Moreover as a part of solving wastewater treatment problems in urban or industrial areas using this plant, a large number of useful byproducts can be developed like animal and fish feed, power plant energy (briquette), ethanol, biogas, composting and fiber board making. In focus to the future aspects of phytoremediation, the utilization of invasive plants in pollution abatement phytotechnologies can certainly assist for their sustainable management in treating waste water.