Variability in Water Capacity of Small-Leaved Linden Associated with Both the Presence of Honeydew and Various Sources of Pollution.

The process of water retention on the surface of the assimilation apparatus of plants is inextricably linked with the water capacity of tree crowns and depends mainly on the condition of the leaf itself. The main objective of the present study was to investigate how the honeydew coverage and the location of trees related to the content of polycyclic aromatic hydrocarbons affected the differences in the capability of small-leaved linden leaves to capture water. Honeydew coverage was determined with the use of AutoCAD, whereas the content of polycyclic aromatic hydrocarbons was determined using gas chromatography. The value of S (water capacity) was much lower before the appearance of honeydew on the leaf than at the peak moment of honeydew collection. This is due to the hydrophobic properties of the substance. It was also found that the content of polycyclic aromatic hydrocarbons (PAH) in leaves varied depending on the distance of trees from pollution sources, and it was found that the amount of PAHs increased with the growth of honeydew on leaves and in locations exposed to pollution. The highest S and the total amount of PAHs occurred with the combination of the largest amount of pyrogenic impurities with the highest amount of honeydew. Combing pollutants from the air by plants is a very important function, but it may also change the physical properties of leaves, such as wettability.

Characteristics of contaminants in the polish-origin bee products and cancer risk assessment.

The aim of this study was to evaluate the concentration of 5-hydroxymethylfurfural (HMF), furfural, polycyclic aromatic hydrocarbons (PAHs), and pesticide residues, as well as assessment of cancer risk of the Polish-origin bee products. The bee product samples were prepared using a modified QuEChERS method, then PAHs and pesticides were analysed by gas chromatography-mass spectrometry (GC-MS), neonicotinoids by high-performance liquid chromatography with a diode array detector (HPLC-DAD), and HMF and furfural by spectrophotometry (HPLC-UV/Vis). The results showed that the highest furfural content was found in bee bread from the northeast part of Poland; moreover, samples obtained from the same region were also characterized with a higher level of HMF. The total sum of PAHs ranged from 324.0 to 866.4 µg/kg; the highest content of PAH4 (the sum of benzo[a]anthracene, chrysene, benzo[b]fluoranthene and benzo[a]pyrene) was 21.0 µg/kg, but only benzo[a]anthracene and chrysene were detected in the samples. Imidacloprid and acetamiprid were found only in bee bread from the northeast part of Poland, while clothianidin was detected in honey samples. The acceptable cancer risk has been calculated for PAHs due to ingestion of honey, while increasing the risk of cancer was calculated for bee bread and bee pollen. Due to the high concentration of PAHs and excessively high recommended consumption dose, regular consumption of bee bread and pollen may pose a severe threat to human health and should be strictly limited.

Levels of Contamination by Pesticide Residues, Polycyclic Aromatic Hydrocarbons (PAHs), and 5-Hydroxymethylfurfural (HMF) in Honeys Retailed in Europe.

Honey is consumed worldwide because of its nutritional, therapeutic and medicinal properties. Generally, honey should reach the consumer in a pure form, but it is often contaminated in various ways. Thus, this study was designed to check for the presence of pesticide residues, polycyclic aromatic hydrocarbon (PAH) levels, and the content of 5-hydroxymethylfurfural (HMF) in selected European honeys (26 samples) obtained from shops. The most frequently detected organochlorine pesticide (OCPs) was 4,4'-DDD, which was found in fourteen honey samples. Slovakian rapeseed honey was most polluted due to the presence of eight OCPs. The presence of organophosphorus pesticides (OPs) was detected in all the analysed samples, and at least one OP in each tested sample exceeded the acceptable limit. PAHs were detected in most of the analysed samples. As for PAH4s, benzo[a]pyrene was found in forest honey from Slovakia and in Polish lime tree honey, whereas wild flower honey from the UK contained the highest level of PAH4. The investigated honeys from Spain and France met the requirements for HMF content, while honeys of Slovak, Italian and Polish origin in most cases exceeded the established levels (40 mg/kg).

Monochloropropanediol esters and glycidyl esters in dietary supplements based on fish oils.

Dietary supplements based on fish oils might be contaminated with thermal processing contaminants, which are generated during the fish oil deodorisation. In the study, 30 samples of dietary supplements were analysed in terms of the occurrence of 3-monochloropropane-1,2-diol esters (3-MCPDE), 2-monochloropropane-1,3-diol esters (2-MCPDE) and glycidyl esters (GE). The results showed that the highest levels of 3-MCPDE (mean: 1461 µg kg-1) as well as 2-MCPDE (mean: 357 µg kg-1) were observed in the products containing shark liver oil. In the case of GE, they were mainly detected in the supplements including shark liver and cod liver oils. Although the results indicated that the consumption of the investigated supplements constituted no more than 1% of tolerable daily intake (TDI), the occurrence of MCPDE and GE in fish oil dietary supplements with a special attention to the origin of ester precursors should be thoroughly investigated in further studies.

Carbohydrate-Based Fat Mimetics Can Affect the Levels of 3-Monochloropropane-1,2-Diol Esters and Glycidyl Esters in Shortbread Biscuits.

Shortbread biscuits have become one of the most desirable snacks in all population groups but due to its high fat content it is searching for new solutions of fat reduction such as the use of carbohydrate-based fat mimetics. However, the thermal processing of food containing lipids in the presence of certain carbohydrates can contribute to the formation of toxic compounds or alter its levels. Therefore, the aim of the study was to evaluate the use of inulin and pectin gels as partial fat replacers (10-40%) in classical shortbread biscuits in a view of the changes of 3-monochloropropane-1,2-diol ester (3-MCPDE) and glycidyl ester (GE) levels after baking, and also after a two-months storage. The experiment showed that the amount of inulin gel higher than 10% promoted the endogenous formation of 3-MCPDE probably due to the higher amount of Maillard products formed in the biscuits, but after two months the levels decreased and were comparable with those transferred from shortening. Pectin did not demonstrate such phenomenon, owing to the presence of bound water in gel. Glycidyl esters were partially decomposed during baking and also during storage, particularly in the samples with the addition of pectin gel, which presumably caused by low stability of GE under acidic conditions resulting from the occurrence of galacturonic acid in pectin.

Analysis of acrylamide, 3-monochloropropane-1,2-diol, its esters and glycidyl esters in carbohydrate-rich products available on the Polish market

Background: Carbohydrate-rich foods, such as breakfast products, snacks and biscuits because of its nutritional or sensory qualities are an inherent part of human diet. However, their production might contribute to the formation of acrylamide, 3-monochloropropane-1,2-diol (3-MCPD) and its esters and glycidyl esters. Objective: The aim of this work was to assess the levels of acrylamide, free and bound 3-MCPD and glycidyl esters in selected carbohydrate-rich, thermal processed products, present on the market in Poland in 2016-2017. Material and Methods: The survey involved 60 samples of snacks, breakfast products and biscuits. Acrylamide and free 3-MCPD was determined using modified QuEChERS approach. Analysis of 3-MCPD and glycidyl esters was based on the acid-catalysed method of sample preparation, derivatisation with PBA and GC-MS analysis. Results: Free 3-MCPD contents were within the values of 9.3-63.3 µg kg-1, with the highest mean content for muesli (33.3 µg kg-1), and the lowest for baby biscuits (11.7 µg kg-1). The levels of bound 3-MCPD were higher (from 9.3 µg kg-1 to 1500 µg kg-1). The highest average content was observed for sugar free biscuits (599 µg kg-1), whereas the lowest for breakfast cereals (50.2 µg kg-1). Glycidyl esters were detected only in four samples with the highest content at the level of 28.8 µg kg-1. The acrylamide levels varied from 195 to 1352 µg kg-1, with the highest content for organic biscuit samples (913 µg kg-1), and the lowest for muesli (348 µg kg-1). Conclusions: Regular consumption of popular snacks such as potato chips, crackers and biscuits may result in risk to human health as the effect of high content of acrylamide or 3-MCPD. Due to a high level of these contaminants detected in some type of breakfast products, and products targeted for children, its consumption should be restricted, especially in younger population groups.

Comparison of different modifications on QuEChERS sample preparation method for PAHs determination in black, green, red and white tea.

The aim of this work was the evaluation of QuEChERS extraction method for polycyclic aromatic hydrocarbon (PAH) determination in various types of tea. In the experiment, different kinds of extraction solvents, sorbents and a final method of sample preparation were compared. The final extracts were analysed by gas chromatography-selected ion monitoring-mass spectrometry. The results suggest that acetonitrile extraction, clean up with SAX and final liquid-liquid extraction was the best combination giving the most purified extracts and acceptable compound recoveries for different types of teas. In the study of real samples, compounds belonging to light PAHs were mostly detected, and heavy polycyclic aromatic hydrocarbons, including benzo[a]pyrene, were not identified in any of samples.

Application of QuEChERS method for simultaneous determination of pesticide residues and PAHs in fresh herbs.

The aim of this study was to evaluate the application of quick, easy, cheap, effective, rugged and safe method for simultaneous determination of polycyclic aromatic hydrocarbons and pesticide residues in fresh herbs. In the experiment two extraction solvents and standard types of sorbents were used. The extracts were analyzed using GC-SIM-MS. The results suggest that acetonitrile is more suitable extraction solvent giving more purified samples and better recovery values (71.6 %-116.9 %) with RSD lower than 15 % for most of the compounds. In real samples pesticides were identified in the samples of parsley, tarragon and lovage. In few samples the pesticide levels exceeded the MRL established by EU.

Analysis of odorous compounds at municipal landfill sites.

The aim of this investigation was to determine odorous compounds in the air over the landfill sites in France and Poland. Air samples were collected by passive and dynamic methods of preconcentration analytes and analysed by GC-MS and GC-FID. The coupling microTD-microGC-MS was also used for on-site analysis of odorous compounds. The achieved results indicated that the concentrations of odorants in the air varied and strongly depended on the sampling site. The highest concentrations were observed at the points situated near biogas wells and above the fresh waste layer. The concentrations were influenced by landfill activities such as failures of the landfill gas collection system, heavy truck traffic, machinery operations and compacting fresh waste.