Antimicrobial activity of cleanser tablets against S. mutans and C. Albicans on different denture base materials.

BACKGROUND: In this study, the antimicrobial activity of three different cleanser tablets on S. mutans and C. albicans adhesion to PMMA, polyamide and 3D printed resin was investigated. METHODS: 40 samples were prepared for PMMA (SR Triplex Hot), polyamide (Deflex) and 3D printed resin (PowerResins Denture) materials and divided into four subgroups for cleansers (Aktident™, Protefix™, Corega™ tablets and distilled water) (n = 5). After the surface preparations were completed, the samples were immersed separately in tubes containing the prepared microorganism suspension and incubated at 37˚C for 24 h. After the incubation, the samples were kept in the cleanser solutions. The samples were then transferred to sterile saline tubes. All the tubes were vortexed and 10 µl was taken from each of them. Sheep blood agar was inoculated for colony counting. The inoculated plates were incubated for 48 h for S. mutans and 24 h for C. albicans. After incubation, colonies observed on all plates were counted. Statistical analyses were done with three-way ANOVA and Tukey's multiple comparison test. RESULTS: Polyamide material registered the highest colony count of S. mutans, whereas PMMA registered the lowest. Significant differences in S. mutans adherence (p = 0.002) were found between the three denture base materials, but no such difference in C. albicans adherence (p = 0.221) was identified between the specimens. All three cleanser tablets eliminated 98% of S. mutans from all the material groups. In all these groups, as well, the antifungal effect of Corega™ on C. albicans was significantly higher than those of the other two cleanser tablets. CONCLUSIONS: According to the study's results, it may be better to pay attention to surface smoothness when using polyamide material to prevent microorganism retention. Cleanser tablets are clinically recommended to help maintain hygiene in removable denture users, especially Corega tablets that are more effective on C. albicans.

Comparison of the Micro-Shear Bond Strength of Resin Cements to CAD/CAM Glass Ceramics with Various Surface Treatments.

This study aimed to compare the effect of acid etching, sandblasting, or silica coating on the micro-shear bond strength of dual-cured resin cements to computer-aided design and computer-aided manufacturing (CAD/CAM) glass ceramic materials. Feldspathic, lithium disilicate, and zirconia-reinforced CAD/CAM ceramics were divided into four groups: control group (C), no surface treatment; hydrofluoric (HF) group, 5% HF acid-etched; sandblasting (SB) group, abraded with 50 µm aluminum oxide (Al2O3) particles; silica-coated (CJ) group, abraded with 30 µm silica-modified Al2O3 particles. Roughness values were obtained by using a profilometer. The cements were condensed on the surface-treated specimens and a micro-shear bond test was conducted. The ceramic material (p < 0.001) and surface treatment type (p < 0.001) significantly affected the micro-shear bond strength values. HF acid etching can be recommended for the surface pretreatment of feldspathic, lithium disilicate, and zirconia-reinforced CAD/CAM ceramics. Better bond strengths can be obtained with HF acid etching than with sandblasting and silica coating.

Comparison of fiber reinforcing methods of composite resin: A flexural strength and stereo microscopy study.

This study aimed to compare the effect of fiber reinforcing methods on the flexural strength and failure modes of indirect composite resins. Based on the reinforcement methods, the bar specimens (3 × 3 × 25 mm) were divided into five groups (n = 20). Glass or polyethylene fibers were used for reinforcement of indirect composite resins. Fibers were either light polymerized and mixed with indirect composite resin or mixed with indirect composite resin after resin application and polymerized together. Indirect composite resin without fiber reinforcement was used as control. All five types of specimens were stored in distilled water at 37°C for 24 h. Half of the specimens were additionally thermocycled. Then the specimens were tested in a three-point bending test. Failure types were examined and categorized by using stereo microscope. The data were analyzed using two-way ANOVA and Tukey HSD test. Flexural strength was found to be significantly higher for fiber-reinforced indirect resin composites than control. However, the fiber-reinforced groups did not present any significant difference. Analysis revealed aging does not affect the flexure strength of fiber reinforcement of indirect composite resin. The study concluded that the flexure strength of indirect composite resins was improved with fiber reinforcement. Different fiber reinforcement methods demonstrated similar effects on the flexure strength of indirect composite resin. Reinforcement with glass or polyethylene fibers presented the potential to improve the mechanical properties of indirect composite resins. RESEARCH HIGHLIGHTS: Flexural strength of indirect composite resins are affected by the reinforcement of composites with glass or polyethylene fibers. Aging with thermocycling has no effect on the flexural strength of the indirect composite resins, however can cause catastrophic failures in material.

Marginal adaptation and fracture strength of endocrowns manufactured with different restorative materials: SEM and mechanical evaluation.

Endocrowns were manufactured using different restorative materials to evaluate the marginal adaptation and fracture strength. Fifty endodontically treated mandibular first molar teeth were divided into five groups (n = 10). Endocrowns were obtained from lithium disilicate glass ceramic ingots by heat-press technique (Group e.max Press: GEP), and from feldspathic blocks (Group Cerec: GC), polymer infiltrated ceramic network blocks (Group Enamic: GE), lithium disilicate glass ceramic blocks (Group e.max CAD: GEC), and zirconia-reinforced glass ceramic blocks (Group Suprinity: GS) by CAD/CAM technique. After thermocycling, marginal adaptation was evaluated under scanning electron microscope at ×200 magnification. The specimens' fracture strengths were tested in universal test machine, and fracture types were evaluated. Statistical analyses were performed with Kruskal-Wallis test. The highest marginal gap value was found in GEP, but no significant differences were determined among the other four groups (p > .05). Significant differences were observed among the groups in terms of fracture strength (p = .019). The fracture strength values of GEC were significantly higher than GE, GC, and GS (p < .05). Values were not significantly different between the GEC and GEP groups (p > .05). CAD/CAM endocrowns showed better marginal adaptation than heat-pressed endocrowns. Clinically acceptable marginal gaps were seen in both endocrown types. Both CAD/CAM and heat-pressed lithium disilicate glass ceramic endocrowns showed higher fracture strength.