A combined toxicological impact on Artemia salina caused by the presence of dust particles, microplastics from cosmetics, and paracetamol.

Environmental pollution poses a significant and pressing threat to the overall well-being of aquatic ecosystems in modern society. This study showed that pollutants like dusts from AC filter, fan wings and Traffic dust PM 2.5 were exposed to Artemia salina in pristine form and in combination. The findings indicated that exposure to multi-pollutants had a detrimental effect on the hatching rates of A. salina cysts. Compared to untreated A. salina, the morphology of adult (7th day old) A. salina changed noticeably after each incubation period (24-120 h). Oxidative stress increased considerably as the exposure duration increased from 24 to 120 h compared to the control group. There was a time-dependent decline in antioxidant enzyme activity and total protein concentration. When all particles were used all together, the total protein content in A. salina decreased significantly. All particles showed a considerable decline in survival rate. Those exposed to traffic dust particles showed significantly higher levels of oxidative stress and antioxidant activity than those exposed to other particles.

Isolation and characterization of microplastics from skin care products; interactions with albumin proteins and in-vivo toxicity studies on Artemia salina.

Skincare products are a significant source of primary microplastics (MPs). This study isolates and characterizes microplastic from two skin care products: face wash (FW-MPs) and face scrub (FS-MPs). Microplastics extracted were around 660 µm in size. The extracted MPs, designated as unground MPs (UG-MPs), were smooth surface and spherical. Ground ones were denoted as the ground MPs (G-MPs) that varied in size and surface shape. G-MPs interacted with bovine serum albumin (BSA) and human serum albumin (HSA). BSA adsorption on FW-MPs was 29%, whereas HSA adsorption was 47%. Contrarily, FS-MPs displayed 17% and 31%. Fluorescence spectroscopy and FE-SEM images showed HSA adsorption on G-MPs was greater than BSA. G-MP interaction changed the life cycle of Artemia salina. UV-Vis and fluorescence spectroscopy were used to study protein adsorption and desorption on G-MPs. A. salina treated to 2.5 mg/mL G-MPs delayed hatching and development and internalized microplastics in the gut at 144 h exposure.