RESUMO
Schizosaccharomyces pombe and Saccharomyces cerevisiae are excellent model organisms to study lifespan. We conducted screening to identify novel genes that, when overexpressed, extended the chronological lifespan of fission yeast. We identified seven genes, among which we focused on SPBC16A3.08c. The gene product showed similarity to Ylr150w of S. cerevisiae, which has affinity for guanine-quadruplex nucleic acids (G4). The SPBC16A3.08c product associated with G4 in vitro and complemented the phenotype of an S. cerevisiae Ylr150w deletion mutant. From these results, we proposed that SPBC16A3.08c encoded for a functional homolog of Ylr150w, which we designated ortholog of G4-associated protein (oga1 (+)). oga1 (+) overexpression extended the chronological lifespan and also decreased mating efficiency and caused both high and low temperature-sensitive growth. Deleting oga1 (+) resulted in caffeine-sensitive and canavanine-resistant phenotypes. Based on these results, we discuss the function of Oga1 on the chronological lifespan of fission yeast.
Assuntos
Proteínas de Ligação a DNA/genética , Quadruplex G , Proteínas de Schizosaccharomyces pombe/genética , Schizosaccharomyces/fisiologia , Cafeína/farmacologia , Canavanina/farmacologia , Clonagem Molecular , Proteínas de Ligação a DNA/metabolismo , Farmacorresistência Fúngica/genética , Deleção de Genes , Regulação Fúngica da Expressão Gênica , Teste de Complementação Genética , Proteínas Quinases/genética , Saccharomyces cerevisiae/efeitos dos fármacos , Saccharomyces cerevisiae/genética , Proteínas de Saccharomyces cerevisiae/genética , Schizosaccharomyces/efeitos dos fármacos , Proteínas de Schizosaccharomyces pombe/metabolismoRESUMO
We report a case of systemic sclerosis with interstitial pneumonia and severe pulmonary hypertension treated with bosentan, an endothelin receptor antagonist. Within a short period of administration of the bosentan the skin sclerosis improved.
Assuntos
Anti-Hipertensivos/uso terapêutico , Hipertensão Pulmonar/tratamento farmacológico , Escleroderma Sistêmico/tratamento farmacológico , Sulfonamidas/uso terapêutico , Anti-Inflamatórios/uso terapêutico , Bosentana , Humanos , Hipertensão Pulmonar/complicações , Doenças Pulmonares Intersticiais/complicações , Doenças Pulmonares Intersticiais/tratamento farmacológico , Masculino , Metilprednisolona/uso terapêutico , Pessoa de Meia-Idade , Doença de Raynaud/complicações , Doença de Raynaud/tratamento farmacológico , Escleroderma Sistêmico/complicaçõesRESUMO
Tinea incognito refers to fungal infection whose clinical appearance is modified or aggravated by administration of systemic or topical corticosteroids. We report a case of pustular psoriasis-like tinea incognito caused by Trichophyton mentagrophytes var. interdigitale under topical corticosteroid therapy. Sequence analysis of the internal transcribed spacer 2 region, in addition to the D1D2 domain of the 26S ribosomal RNA gene, was helpful in identifying the fungal species.
