RESUMO
Feline calicivirus (FCV) is a pathogenic microorganism that causes upper respiratory diseases in cats. Recently, an FCV infection with a high mortality rate has been confirmed, and there is need to develop a treatment for cases of acute infection. We evaluated whether the replication of FCV could be prevented by RNA interference. For this study, we designed an siRNA targeted to the polymerase region of the strain FCV-B isolated from a cat that died after exhibiting neurological symptoms. Cells transfected with siR-pol dose-dependently suppressed the replication of FCV-B. siR-pol suppressed its replication by suppressing the target viral RNA.
Assuntos
Infecções por Caliciviridae/veterinária , Calicivirus Felino/fisiologia , Doenças do Gato/virologia , Genes pol/genética , RNA Interferente Pequeno/genética , Replicação Viral/genética , Animais , Infecções por Caliciviridae/terapia , Infecções por Caliciviridae/virologia , Calicivirus Felino/genética , Calicivirus Felino/isolamento & purificação , Doenças do Gato/mortalidade , Doenças do Gato/terapia , Gatos , Linhagem Celular , Efeito Citopatogênico Viral , Genoma Viral/genética , Interferência de RNA , RNA Viral/genética , Fatores de Tempo , Transfecção/veterináriaRESUMO
As a preventive action plan against gastroenteritis caused by the Norovirus (NV), we studied hand hygiene effects using with three hand rubbing products, four wet wipe products, and two functional water types using Feline Calicivirus as a Norovirus surrogate. After treatment using antiseptic hand rubbing products containing chlorhexidine, quaternary ammonium, and povidone-iodine, high inactivation detected by TCID50 was observed compared to products containing povidone-iodine, although no difference was seen in viral removal measured by the amount of viral genome copies in real-time-PCR. Among wet wipes soaked in chlorhexidine, quaternary ammonium, benzoic acid and PHMB, two groups showed viral inactivation and removal. Two products were more effective for functional water, viral decrease was seen in rinsing in running electrolyzed acid water and handwashing by soap. Results underscore the importance of selection in hand washing metheds (alternative soap and also) in preventing viral gastroenteritis.
Assuntos
Anti-Infecciosos Locais/farmacologia , Calicivirus Felino/efeitos dos fármacos , Mãos/virologia , Norovirus/efeitos dos fármacos , Ácido Benzoico/farmacologia , Clorexidina/farmacologia , Desinfecção das Mãos/métodos , Humanos , Hidroximercuribenzoatos/farmacologia , Povidona-Iodo/farmacologia , Compostos de Amônio Quaternário/farmacologia , Inativação de VírusRESUMO
Viral gastroenteritis caused by Norovirus (NV) mainly appears during the winter season. In fact, outbreaks and patients with NV gastroenteritis are the major cause of community disease in the winter. Strategies to avoid gastroenteritis caused by NV are thus needed. No effective method for evaluating virus inactivation and removal exists for of NV, which cannot be cultured using cell-lines. Trials using Feline Calici Virus (FCV; a member of the calicivirus family) as a NV surrogate have been conducted by culturing FCV in CRFK cells. By washing one's hands, about 99% of the viruses can be removed, compared with simply rinsing one's hands in running water. Washing one's hands with alcohol, chlorhexidine, quaternary ammonium, or 3 other kinds of hand soaps (containing povidone-iodine, triclosan, and isopropylmethyl phenol, respectively), was also effective for removing viruses. These results suggest that washing one's hands may be an effective method of preventing viral gastroenteritis.
