Investigation of Genetic Polymorphisms in BMP2, BMP4, SMAD6, and RUNX2 and Persistent Apical Periodontitis.

INTRODUCTION: This study aimed to evaluate the interplay among single-nucleotide polymorphisms (SNPs) in the encoding genes BMP2, BMP4, SMAD6, and RUNX2 in persistent apical periodontitis (PAP). METHODS: In this multicentric study, 272 patients diagnosed with pulp necrosis with apical periodontitis before root canal therapy who attended regular follow-up visits for at least 1 year were screened. Periapical radiographs and clinical aspects were evaluated, and the participants were classified as PAP (n = 110) or repaired (n = 162). Genomic DNA was used for the genotyping of the following SNPs: rs1005464 and rs235768 in bone morphogenetic protein 2 (BMP2), rs17563 in bone morphogenetic protein 4 (BMP4), rs2119261 and rs3934908 in SMAD family member 6 (SMAD6), and rs59983488 and rs1200425 in runt-related transcription factor 2 (RUNX2). The chi-square test was used to compare genotype distributions between groups. The multifactor dimensionality reduction method was applied to identify SNP-SNP interactions. The alpha for all the analysis was 5%. RESULTS: The multifactor dimensionality reduction suggested the rs235768 in BMP2 and rs59983488 in RUNX2 as the best SNP-SNP interaction model (cross-validation = 10/10, testing balanced accuracy = 0.584, P = .026) followed by rs17563 in BMP4 and rs2119261 in SMAD6 (cross validation = 10/10, testing balanced accuracy = 0.580, P = .031). In the rs235768 in BMP2 and rs59983488 in RUNX2 model, the high-risk genotype was TT + TT (odds ratio = 4.36; 95% confidence interval, 0.44-42.1). In model rs17563 in BMP4 and rs2119261 in SMAD6, GG + TT (odds ratio = 2.63; 95% confidence interval, 0.71-11.9) was the high-risk genotype. CONCLUSIONS: The interactions between rs235768 in BMP2 and rs59983488 in RUNX2 and between rs17563 in BMP4 and rs2119261 in SMAD6 are associated with PAP, suggesting that an interplay of these SNPs is involved in the higher risk of developing PAP.

Association between Estrogen, Vitamin D and Microrna17 Gene Polymorphisms and Periapical Lesions.

This study evaluated the association between polymorphisms in genes encoding estrogen receptors 1 (ESR1) and 2 (ESR2), vitamin D receptor (VDR) and in microRNA17 (which binds to ESR1 and VDR) with persistent apical periodontitis (PAP) after the endodontic treatment. We included 162 patients who completed endodontic treatment at least one year ago and presented apical periodontitis at the beginning of the root canal therapy. Clinical and radiographic exams were performed to evaluate the presence of PAP or healthy periradicular tissues (healed). Saliva samples were collected as a genomic DNA. The genotyping of ESR1 (rs2234693 and rs9340799), ESR2 (rs1256049 and rs4986938), VDR (rs739837 and rs2228570) and miRNA17 (rs4284505) were performed by real-time PCR. Chi-square test was used to the distribution of genotype and allele frequencies. Haplotype analysis was also performed. Eighty-nine patients were included in the "healed" group and 73 in the "PAP" group. No association was found between the allelic and genotypic polymorphisms studied and PAP (p>0.05). Haplotype analysis also did not demonstrated an association (p>0.05). In conclusion, the genetic polymorphisms in ESR1, ESR2, VDR and miRNA17 are not associated with PAP.

Genetic Polymorphisms in RANK and RANKL are Associated with Persistent Apical Periodontitis.

INTRODUCTION: The outcome of root canal treatment has been reported as intimately related to the host response. Genetic polymorphisms might be associated with apical periodontitis repair. The aim of this study was to evaluate the association between receptor activator of nuclear factor kappa B (RANK), receptor activator of nuclear factor kappa B ligand (RANKL), and osteoprotegerin (OPG) genetic polymorphisms with persistent apical periodontitis (PAP) in Brazilian subjects. METHODS: Subjects with at least 1 year of follow-up after nonsurgical root canal therapy were recalled. Sixty-four subjects with signs/symptoms of PAP and 86 subjects with root canal-treated teeth exhibiting healthy periradicular tissues (healed) were included. Genomic DNA was extracted from saliva and used for RANK (rs3826620), RANKL (rs9594738), and OPG (rs2073618) genotyping by real-time exact tests, and odds ratio were implemented using Epi Info 3.5.2 (Centers for Disease Control and Prevention, Atlanta, GA). A logistic regression analysis was also performed using the time of follow-up as the covariate. All tests were performed with an established alpha of 0.05 (P = .05). RESULTS: An association between allele distribution and the polymorphism in RANK was observed. Subjects who carry the T allele had a lower risk of having PAP (P < .05). In RANKL polymorphism, the genotype distribution was statistically significant different between the PAP and healed groups (P = .05). The time of follow-up was associated with PAP (P < .05). In the logistic regression analysis using time as a covariant, RANK (P < .05) and RANKL (P < .05) were associated with PAP. The polymorphism rs2073618 in OPG was not associated with PAP (P > .05). CONCLUSIONS: These findings suggest that polymorphisms in RANK and RANKL genes are associated with PAP.

Influence Of Genetic Polymorphisms In Genes Of Bone Remodeling And Angiogenesis Process In The Apical Periodontitis.

Persistent apical periodontitis (AP) is a situation involving an inflammatory and immune response caused mainly by anaerobic polymicrobial infection of the root canal system and the outcome and follow-up of the root canal treatment has been reported as intimately related to host response. The apical periodontitis repair might be associated with genetic polymorphisms. This study aimed to evaluate the association between HIF1A genetic polymorphisms (rs2301113 and rs2057482) with PAP in Brazilian patients. Subjects with at least 1 year of follow-up after root canal therapy (RCT) were recalled. Sixty-four subjects with signs/symptoms of PAP and 84 subjects with root canal-treated teeth exhibiting healthy perirradicular tissues (healed) were included. Genomic DNA was extracted from saliva and used for HIF1A genotyping by real-time PCR. Genotype and allele frequencies were compared by c2 or Fisher's exact tests and odds ratio was implemented, using Epi Info 3.5.2. All tests were performed with an established alpha of 0.05. There was no association between allele and genotype distribution for HIF1As polymorphisms and PAP (p>0.05). The genetic polymorphisms in HIF1A were not associated with persistent apical periodontitis.

Influence of genetic polymorphisms in genes of bone remodeling and angiogenesis process in the apical periodontitis

Abstract Persistent apical periodontitis (AP) is a situation involving an inflammatory and immune response caused mainly by anaerobic polymicrobial infection of the root canal system and the outcome and follow-up of the root canal treatment has been reported as intimately related to host response. The apical periodontitis repair might be associated with genetic polymorphisms. This study aimed to evaluate the association between HIF1A genetic polymorphisms (rs2301113 and rs2057482) with PAP in Brazilian patients. Subjects with at least 1 year of follow-up after root canal therapy (RCT) were recalled. Sixty-four subjects with signs/symptoms of PAP and 84 subjects with root canal-treated teeth exhibiting healthy perirradicular tissues (healed) were included. Genomic DNA was extracted from saliva and used for HIF1A genotyping by real-time PCR. Genotype and allele frequencies were compared by c2 or Fisher's exact tests and odds ratio was implemented, using Epi Info 3.5.2. All tests were performed with an established alpha of 0.05. There was no association between allele and genotype distribution for HIF1As polymorphisms and PAP (p>0.05). The genetic polymorphisms in HIF1A were not associated with persistent apical periodontitis.

Resumo A periodontite apical persistente (PAP) é uma condição que envolve uma resposta inflamatória e imunológica causada principalmente por infecções polimicrobianas de origem anaeróbia no sistema de canais radiculares, tornando o resultado e o acompanhamento do tratamento do canal radicular intimamente relacionados à resposta do hospedeiro. O reparo da periodontite apical pode estar associado a polimorfismos genéticos. Este estudo teve como objetivo avaliar a associação entre os polimorfismos genéticos do HIF1A (rs2301113 e rs2057482) com a PAP em pacientes brasileiros. Indivíduos com pelo menos 1 ano de acompanhamento após o tratamento do canal radicular (TCR) foram agendados para consulta de acompanhamento. Sessenta e quatro indivíduos com sinais/sintomas de PAP e 84 indivíduos com dentes tratados endodonticamente e tecidos perirradiculares saudáveis (cicatrizados) foram incluídos no presente estudo. O DNA genômico foi extraído da saliva e utilizado para a genotipagem do HIF1A por PCR em tempo real. O genótipo e as frequências alélicas foram comparados por teste c2 ou exato de Fisher e odds-ratio foi implementado por meio do software Epi Info 3.5.2. Todos os testes realizados foram estabelecidos com a=0,05. Não houve associação entre alelo e distribuição genotípica para polimorfismos do HIF1A e PAP (p> 0,05). Os polimorfismos genéticos em HIF1A não foram associados à periodontite apical persistente.

Association Between Apical Periodontitis and TNF-α -308 G>A Gene Polymorphism: A Systematic Review and Meta-Analysis.

Currently, investigations have focused on the identification of Single Nucleotide Polymorphisms (SNP) involved in host response and its ability to generate an immunity deficiency. The aim of this study was to perform a systematic review (SR) and meta-analysis to evaluate the association between TNF-α -308 G>A polymorphism and apical periodontitis (AP) phenotypes. A broad search for studies was conducted. The following databases were used: PubMed, Scopus, Web of Science, and VHL (Medline, SciELO, Ibecs, and Lilacs). The MeSH terms "Periapical Periodontitis," "Periapical Abscess," "Polymorphism, Genetic," and "Polymorphism, Single Nucleotide" were used. MeSH synonyms, related terms, and free terms were included. Clinical investigations of individuals with different AP phenotypes in permanent teeth were selected. After application of the eligibility criteria, selected studies were qualified by assessing their methodological quality. A fixed effect model was used for the meta-analysis. The initial search identified 71 references. After excluding duplicate abstracts, 33 were selected. From these, two were eligible for quality assessment and were classified as being of moderate evidence. The included studies did not demonstrate association between AP and TNF-α -308 G>A SNP. However, the meta-analysis demonstrated an association between the genotype distribution and AP phenotype (OR= 0.49; confidence interval= 0.25, 0.96; p=0.04). The role of TNF-α -308 G>A SNP in AP phenotypes is debatable. Further studies are needed to confirm and understand the underlying mechanisms of the identified association.

Association Between Apical Periodontitis and TNF- alpha -308 G>A Gene Polymorphism: A Systematic Review and Meta-Analysis

Abstract Currently, investigations have focused on the identification of Single Nucleotide Polymorphisms (SNP) involved in host response and its ability to generate an immunity deficiency. The aim of this study was to perform a systematic review (SR) and meta-analysis to evaluate the association between TNF-α -308 G>A polymorphism and apical periodontitis (AP) phenotypes. A broad search for studies was conducted. The following databases were used: PubMed, Scopus, Web of Science, and VHL (Medline, SciELO, Ibecs, and Lilacs). The MeSH terms "Periapical Periodontitis," "Periapical Abscess," "Polymorphism, Genetic," and "Polymorphism, Single Nucleotide" were used. MeSH synonyms, related terms, and free terms were included. Clinical investigations of individuals with different AP phenotypes in permanent teeth were selected. After application of the eligibility criteria, selected studies were qualified by assessing their methodological quality. A fixed effect model was used for the meta-analysis. The initial search identified 71 references. After excluding duplicate abstracts, 33 were selected. From these, two were eligible for quality assessment and were classified as being of moderate evidence. The included studies did not demonstrate association between AP and TNF-α -308 G>A SNP. However, the meta-analysis demonstrated an association between the genotype distribution and AP phenotype (OR= 0.49; confidence interval= 0.25, 0.96; p=0.04). The role of TNF-α -308 G>A SNP in AP phenotypes is debatable. Further studies are needed to confirm and understand the underlying mechanisms of the identified association.

Resumo Atualmente as investigações têm focado na identificação de Polimorfismos de Núcleo Único (SNP) envolvidos na resposta do hospedeiro e sua capacidade de gerar uma deficiência de imunidade. O objetivo deste estudo foi realizar uma revisão sistemática (RS) e uma meta-análise para avaliar a associação entre o polimorfismo do gene TNF-α (-308 G> A) e os fenótipos de periodontite apical (PA). Esta RS foi registrada na base de dados PROSPERO, CRD42016035688, e foi conduzida seguindo o método PRISMA. Foi realizada uma ampla pesquisa de estudos. Foram utilizadas as seguintes bases de dados: PubMed, Scopus, Web of Science e BVS (Medline, SciELO, Ibecs e Lilacs). Foram utilizados os seguintes descritores (MeSH) "Periapical Periodontitis," "Periapical Abscess," "Polymorphism, Genetic," and "Polymorphism, Single Nucleotide". Sinônimos, termos relacionados e termos livres foram incluídos. Foram selecionadas investigações clínicas de indivíduos com diferentes fenótipos de PA em dentes permanentes. Após a aplicação dos critérios de elegibilidade, os estudos selecionados foram classificados através da avaliação da qualidade metodológica. Foi utilizado um modelo de efeito fixo para a metanálise. A pesquisa inicial identificou 71 referências. Após a exclusão de resumos duplicados, 33 foram selecionados. Destes, dois eram elegíveis para a avaliação da qualidade e foram classificados como sendo de evidência moderada. Os estudos incluídos não demonstraram associação entre PA e o TNF-α-308 G>A. No entanto, a metanálise demonstrou associação entre a distribuição do genótipo e os fenótipos da PA (OR = 0,49, intervalo de confiança=0,25, 0,96; p=0,04). O papel do TNF-α-308 G>A em fenótipos da PA é discutível. São necessários mais estudos para confirmar e compreender os mecanismos subjacentes à associação identificada.

Simetria gengival na região estética ­ estudo transversal com uma população brasileira / Gingival symmetry in the esthetic zone ­ a cross-sectional study with a Brazilian population

Objetivo: avaliar o padrão da disposição da margem gengival (simétrica ou assimétrica) na zona estética. Material e métodos: foram avaliados 55 adultos jovens brasileiros, de ambos os sexos, estudantes do curso de graduação em Odontologia da Faculdade de Nova Friburgo, na faixa etária entre 18 e 25 anos. Foram avaliados incisivos centrais superiores direito e esquerdo, por um único examinador especialista em Periodontia, considerando os seguintes parâmetros clínicos: índice de placa, sangramento a sondagem e profundidade clínica de sondagem, coletados nos sítios distal, vestibular e mesial de cada elemento dentário estudado. Foram realizadas, ainda, análises morfométricas quanto à disposição das margens gengivais (simetria ou assimetria) entre incisivos centrais (direito e esquerdo), através de avaliação visual em fotos, utilizando modelos zocalados para uma segunda avaliação, objetivando fidelizar a avaliação antes realizada nas fotos. Resultados: o incisivo central direito foi responsável por 64% das relações assimétricas observadas. A assimetria das margens gengivais, entre incisivos centrais, foi observada em 25,45%, não havendo diferença estatisticamente significante em relação ao sexo. Conclusão: o reconhecimento dos aspectos gengivais (simetria gengival) e sua relação com elementos dentais tornam-se indispensáveis, visto que no processo de restauração é fundamental o entendimento do normal, possibilitando prever as dificuldades encontradas no processo de reconstrução de estruturas alteradas.

Objective: to evaluate the gingival margin disposition (symmetry of the lack of) in the esthetic zone. Material and methods: 55 Brazilian dental students (18-25 years-old) from both genders were evaluated at the Nova Friburgo Dental School. The maxillary right and left central incisors were examiner by an single specialist in Periodontics, considering the following parameters: plaque index, bleeding on probing, pocket probing depth, collected at the distal, buccal, and mesial sites of each element. Also, morphometric analyses were performed regarding the gingival margin disposition through photographic records and standardized stone casts to confirm the data obtained. Results: the maxillary right central incisor was responsible for 64% of asymmetries. The asymmetry between the central incisors was observed in 25.45% being not statistically different between genders. Conclusion: the identification of gingival aspects (gingival symmetry) and its relationship with the dental elements are invaluable to standardize what can be considered as a reference to normal standards, while it still predicts the drawbacks found in the reconstructive process of altered tissues.

O uso do protetor bucal nas artes marciais: consciência e atitude / Mouthguard use in martial arts: awareness and attitude / El uso del protector bucal en las artes marciales: conciencia y actitud

Introdução: O uso de protetores bucais durante a prática de esportes de contato é de fundamental importância como medida preventiva dos traumatismos orofaciais. Objetivo: Realizar um levantamento quanto a utilização do protetor bucal entre praticantes de artes marciais, assim como verificar qual é a percepção que tais esportistas têm sobre sua importância e função. Métodos: Realizou-se uma pesquisa descritiva e transversal, de caráter investigativo, composta por 231 praticantes de três diferentes modalidades de artes marciais: Jiu-jítsu, Tae kwon do e Muay Thai, nas cidades de Nova Friburgo-RJ e Macaé-RJ, Brasil, por meio de um questionário estruturado. Os dados foram tabulados no programa estatístico SPSS 16.0. Foi realizada a frequência, sendo aplicado, ainda, o teste qui-quadrado (p<0,05), para estabelecer relação entre variáveis de interesse. Resultados: Os dados indicaram que 60,6% dos entrevistados nunca receberam informações sobre o traumatismo orofacial durante a prática esportiva e que 46,3% dos esportistas já sofreram algum tipo de traumatismo orofacial. A maioria dos atletas (93,9%) considera importante o uso do protetor bucal, porém este dado é desproporcional ao percentual de usuários do dispositivo: 78,7% no Muay Thai, 60,9% no Jiu-jítsu e 47,5% no Tae kwon do (p< 0,01). Dos protetores bucais existentes no mercado, o mais utilizado é o termoplástico ou tipo II (52,5%) em todas as modalidades de lutas avaliadas. O protetor bucal personalizado causou menor percentual de interferência no rendimento durante a prática esportiva quando comparado com os outros tipos de protetores (p<0,07). Conclusão: A maioria dos esportistas reconhece a importância do uso do protetor bucal, apesar de relatar não ter recebido ...

Introduction: The use of mouthguard during contact sports is crucial for preventing orofacial trauma. Objective: To conduct a survey regarding the use of mouthguards among martial arts practitioners as well as find what is the perception that these athletes have about its importance and function. Method: A descriptive cross-sectional survey of investigative character was conducted with 231 practitioners of three different kinds of martial arts (Brazilian Jiu-Jitsu, Muay Thai and Tae Kwon Do) in the cities of Nova Friburgo and Macaé, in Rio de Janeiro, Brazil, through a structured questionnaire. The data was analyzed in the SPSS 16.0 statistical software. The frequency was obtained and the Chi-square Test applied (p<0.05) to establish association among variables of interest. Results: The data indicated that 60.6% of the respondents had never received information about orofacial trauma during sports practice and that 46.3% of the athletes have suffered some sort of orofacial injury. Most athletes (93.9%) consider important to use the mouth guard, but this datum is disproportionate to the percentage of users of the device: 78.7% in Muay Thai, 60.9% in Jiu-Jitsu and 47.5% in Tae Kwon Do (p<0.01). Among the mouthguards on the market, the most widely used is the thermoplastic or type II (52.5%) in all forms of wrestling evaluated. The custom mouthguard caused the lowest percentage of interference of performance during sports practice when compared with other protective devices (p<0.07). Conclusion: Most athletes recognize the importance of using a mouthguard, although they reported not having received information about orofacial trauma during sports practice; most athletes use thermoplastic mouthguard; it is necessary to disseminate information about the benefits of using custom mouthguard (type III) as well as encourage the use of this particular type by all practitioners of martial arts. .

Introducción: El uso de protectores bucales durante la práctica de deportes de contacto es de fundamental importancia como medida preventiva de los traumatismos orofaciales. Objetivo: Realizar un levantamiento sobre el uso del protector bucal entre practicantes de artes marciales, bien como verificar cuál es la percepción que tales deportistas tienen sobre su importancia y función. Métodos: Se realizó una encuesta descriptiva y transversal, de carácter investigativo, compuesta por 231 practicantes de tres diferentes modalidades de artes marciales: Jiu-jitsu, Tae kwon do y Muay Thai, en la ciudades de Nova Friburgo-RJ y Macaé-RJ, Brasil, por medio de un cuestionario estructurado. Los datos fueron tabulados en el programa estadístico SPSS 16.0. Fue realizada la frecuencia, siendo aplicado, además, el test chi-cuadrado (p<0,05), para establecer relación entre variables de interés. Resultados: Los datos indicaron que 60,6% de los entrevistados nunca recibieron informaciones sobre el traumatismo orofacial durante la práctica deportiva y que 46,3% de los deportistas ya sufrieron algún tipo de traumatismo orofacial. La mayoría de los atletas (93,9%) considera importante el uso del protector bucal, aunque este dato es desproporcional al porcentual de usuarios del dispositivo: 78,7% en Muay Thai, 60,9% en Jiu-jitsu y 47,5% en Tae kwon do (p< 0,01). De los protectores bucales existentes en el mercado, el más utilizado es el termoplástico o tipo II (52,5%) en todas las modalidades de lucha evaluadas. El protector bucal personalizado causó menor porcentual de interferencia en el rendimiento durante la práctica deportiva cuando comparado con los otros tipos de protectores (p<0,07). Conclusión: La mayoría de los deportistas reconoce la importancia del uso del protector bucal, a pesar de relatar no haber recibido informaciones ...