RESUMO
From 1986 to 91, 174 dialysis patients were studied. The prevalence of previous HBV infection and of chronic carriers was 33.3 and 4.6%, respectively. Immunization rate after vaccination (3 doses) was 63%. In 1991, we proposed a vaccination with 4 doses and recommend a 6-monthly anti-HBs evaluation to assess the timing of any booster dose needed.
Assuntos
Hepatite B/transmissão , Diálise Renal/efeitos adversos , Idoso , Portador Sadio/prevenção & controle , Infecção Hospitalar/prevenção & controle , Infecção Hospitalar/transmissão , Feminino , Hepatite B/prevenção & controle , Anticorpos Anti-Hepatite B/sangue , Vacinas contra Hepatite B , Vírus da Hepatite B/imunologia , Hepatite Crônica/prevenção & controle , Humanos , Masculino , Pessoa de Meia-Idade , Vacinas contra Hepatite Viral/uso terapêuticoRESUMO
In our Dialytic Unit, the prevalence of antibodies to HCV (anti-HCV) was 16.4% in 1990 (73 hemodialyzed) and 21% in 1991 (105 hemodialyzed). The incidence of seroconversion was 13.1%. The transaminase behavior was similar in a group of anti-HCV-positive and a group of anti-HCV-negative patients and with previous HBV infection. Blood transfusions and dialytic age did not affect anti-HCV positivity.
Assuntos
Hepatite C/etiologia , Diálise Renal/efeitos adversos , Adulto , Idoso , Alanina Transaminase/sangue , Aspartato Aminotransferases/sangue , Hepacivirus/imunologia , Anticorpos Anti-Hepatite/sangue , Hepatite C/imunologia , Hepatite C/transmissão , Humanos , Pessoa de Meia-Idade , Fatores de Risco , Reação TransfusionalRESUMO
A rapid single-step procedure for the isolation of low molecular weight DNA using guanidinium thiocyanate and phenol as protein denaturants is described and applied for the detection of specific hepatitis B virus (HBV) DNA sequences from serum samples by the polymerase chain reaction (PCR). The novel technique is efficient and, when compared to the standard proteinase K/phenol/chloroform method has the advantage of being faster and easily adaptable to the routine processing of a high number of clinical samples by PCR and spot hybridization techniques.