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1.
Pol J Pathol ; 67(2): 114-21, 2016 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-27543865

RESUMO

Thin basement membrane disease is more common than IgA nephropathy or Alport syndrome, which are also associated with the presence of erythrocyturia. Very few reports on the disorder are available in the Polish literature. The objective of this work was to analyze the results from 83 patients with thin basement membrane syndrome as well as to formulate a proposal of strict morphological assessment criteria for the disorder. Attention was drawn to the requirement of thickness of the lamina densa rather than the entire basement membrane thickness and a sufficiently high number of loops featuring thinned lamina densa, namely at least 80% of loops, being taken into account. Occurrence of other morphological changes associated with the disorder and clinical symptoms other than erythrocyturia was also highlighted.


Assuntos
Hematúria/patologia , Rim/patologia , Adolescente , Criança , Pré-Escolar , Feminino , Imunofluorescência , Humanos , Rim/ultraestrutura , Masculino , Microscopia Eletrônica de Transmissão
3.
Histol Histopathol ; 20(1): 83-9, 2005 01.
Artigo em Inglês | MEDLINE | ID: mdl-15578426

RESUMO

In soft tissue sarcomas, the most important prognostic criteria include extent of malignancy (G), size of the tumour and intensity of Ki-67 antigen expression. In recent times expression of metallothionein (MT) in cells of some malignant processes of epithelial origin was found to correlate with intensity of Ki-67 antigen expression and to carry a possible prognostic significance. The present study aimed at a demonstration of prognostic value of MT expression and at comparing it with Ki-67 antigen expression and G grade in selected soft tissue sarcomas. Immunohistochemical studies were performed on paraffin sections in 54 cases of malignant fibrous histiocytoma (MFH), 18 cases of liposarcoma and 20 cases of synovial sarcoma. The extent of MT and Ki-67 antigen expression was evaluated and an attempt was made to correlate the results with each other and with grade of the tumour. Expression of MT was evident both in the cytoplasm and in cell nuclei of all studied sarcomas. The most pronounced MT expression was noted in MFH-type tumours. The extent of Ki-67 antigen expression was similar in MFH and liposarcoma and was the lowest in synovial sarcoma. In MFH, liposarcoma and synovial sarcoma a pronounced positive correlation was documented between expression of MT and Ki-67 antigen (r=0.85; p<0.001; r=0.93, p<0.0001; r=0.79, p<0.0001). In all types of the tumours a positive relation was detected between MT expression, expression of Ki-67 and G grade of malignancy in the tumour. Moreover, patients with higher MT expression in the studied tumours demonstrated a shorter survival. MT expression in soft tissue tumours of MFH, liposarcoma and synovial sarcoma type strongly correlated with intensity of proliferation (Ki-67) and G grade and could be useful in defining the extent of malignancy and in prognostic appraisal in the tumours.


Assuntos
Antígeno Ki-67/genética , Metalotioneína/genética , Sarcoma/diagnóstico , Humanos , Imuno-Histoquímica , Antígeno Ki-67/biossíntese , Metalotioneína/biossíntese , Prognóstico , Sarcoma/patologia
4.
Pol J Pathol ; 52(1-2): 27-36, 2001.
Artigo em Inglês | MEDLINE | ID: mdl-11505678

RESUMO

Electron microscopy morphometric studies were carried out on material obtained from children with minimal change disease (MCD), mesangioproliferative glomerulonephritis (GNMES) and focal segmental glomerulosclerosis (FSGS). The results indicated that an increase in the volume of the matrix in mesangioproliferative glomerulonephritis was due to an increase in the number of cells. The amount of produced matrix in mesangioproliferative glomerulonephritis was proportional to the number of cells in mesangium (so there is no overproduction of matrix). The ratios of mesangial matrix volume to cell volume and matrix volume to the volume of the entire mesangial region in MCD and GNMES were similar and differed significantly from the ratios found in FSGS. The present findings indicate that morphometric studies may be useful in the early diagnosis of glomerular sclerosis. This is particularly significant in cases where light microscopic examination fails to reveal changes indicative of glomerular sclerosis.


Assuntos
Glomerulonefrite Membranoproliferativa/diagnóstico , Glomerulonefrite Membranosa/diagnóstico , Glomerulosclerose Segmentar e Focal/diagnóstico , Glomérulos Renais/ultraestrutura , Pré-Escolar , Feminino , Humanos , Masculino , Microscopia Eletrônica/métodos
5.
Pol J Pathol ; 52(1-2): 37-46, 2001.
Artigo em Inglês | MEDLINE | ID: mdl-11505679

RESUMO

Clinical and morphological analysis (including morphometric studies of electron microscopic material) was made in 15 children with MCD and 15 children with GNMES. In both groups, an early phase of FSGS was suspected on the basis of electron microscopic studies. Moreover, analysis included the results obtained in 13 children with the diagnosis of FSGS and in whom repeated biopsies were performed. In most of them, MCD or GNMES was diagnosed from the first biopsy. In most children in whom electron microscopic studies revealed an increase in matrix area in some mesangial regions, thereby suggesting an early stage of glomerular sclerosis, the results of morphometric studies resembled or were identical to the results obtained from a control group with the established diagnosis of FSGS. These findings indicate that morphometric studies of electron microscopic material are significant. The results, when compared with the clinical data, confirm the usefulness of such a diagnostic procedure.


Assuntos
Glomerulonefrite Membranoproliferativa/diagnóstico , Glomerulosclerose Segmentar e Focal/diagnóstico , Glomérulos Renais/ultraestrutura , Nefrose Lipoide/diagnóstico , Pré-Escolar , Feminino , Humanos , Lactente , Masculino , Microscopia Eletrônica/métodos
6.
Folia Histochem Cytobiol ; 37(3): 167-72, 1999.
Artigo em Inglês | MEDLINE | ID: mdl-10482248

RESUMO

PTHrP is a HHM-inducing peptide. It exhibits certain structural similarity to PTH and the two hormones may act through the same receptors. PTHrP is known to be produced in various tissues as well as during development. In this study we decided to immunocytochemically demonstrate PTHrP in normal skin and squamous cell carcinomas as well as in parotid glands (normal, inflamed and neoplastic). In the skin, PTHrP expression was demonstrated in epidermis and in smooth muscle cell layer of blood vessels. In squamous cell carcinomas, the expression was noted in foci of keratinization. In parotid glands, the peptide was localised in excretory ducts and in blood vessels, while inflammation of the gland and its tumours resulted most frequently in the less intense immunoreaction. The results are consistent with those of other authors. The novel observations include demonstration of PTHrP expression in myoepithelial cells of sweat glands and in parotid glands, where it may be involved in the control of their contractile activity.


Assuntos
Músculos/citologia , Glândula Parótida/citologia , Proteínas/análise , Glândulas Sudoríparas/citologia , Carcinoma de Células Escamosas/química , Carcinoma de Células Escamosas/patologia , Epiderme/metabolismo , Células Epiteliais/química , Humanos , Imuno-Histoquímica , Inflamação , Músculos/química , Proteínas de Neoplasias/análise , Hormônio Paratireóideo/análise , Proteína Relacionada ao Hormônio Paratireóideo , Glândula Parótida/metabolismo , Neoplasias Parotídeas/química , Neoplasias Parotídeas/patologia , Neoplasias Cutâneas/química , Neoplasias Cutâneas/patologia , Glândulas Sudoríparas/metabolismo
7.
Pol J Pathol ; 50(3): 163-75, 1999.
Artigo em Inglês | MEDLINE | ID: mdl-10624118

RESUMO

Clinical and morphological analysis was made to assess 9 cases of minimal change disease (MCD) and 30 cases of mesangial glomerulonephritis (GNMES) recognized by light microscopy with unfavourable course. Case selection was based exclusively on the clinical course suggesting a possibility of early sclerosis (long-term steroid resistance, frequent recurrences, rare short-lasting remissions, hypertension, renal failure). It was found that the unfavourable clinical course was clearly more frequently associated with electron microscopic than light microscopic changes. Marked increase of the matrix was observed also in those glomeruli in which light microscopy did not reveal any changes or only the signs of immaturity. It was also noticed that in those cases in which the assessment of mesangial matrix increase (which means the onset of sclerosis) is not certain, it is useful to make a morphometric analysis of electron microscopic material.


Assuntos
Mesângio Glomerular/ultraestrutura , Glomerulonefrite Membranoproliferativa/patologia , Nefrose Lipoide/patologia , Adolescente , Pré-Escolar , Glomerulosclerose Segmentar e Focal/patologia , Humanos , Lactente
8.
Pol Arch Med Wewn ; 102(6): 1089-94, 1999 Dec.
Artigo em Polonês | MEDLINE | ID: mdl-11072546

RESUMO

Focal segmental glomerulosclerosis (FSGS) is a glomerular disease of varying severity. Most patients, however, develop end-stage renal failure within 10 years from clinical onset. In this retrospective study, the outcome of immunosuppressive treatment in 22 adult patients with biopsy-proven primary FSGS was evaluated. Eleven patients were treated with prednisone, azathioprine and chlorambucil (group A) and 11 with prednisone and pulse cyclophosphamide (group B). The nephrotic syndrome (NS) was found in 4 patients from the group A and in 3 patients from the group B, arterial hypertension in 8 and 9 patients, respectively. During the follow-up lasting about 50 months as the mean, 70% of the patients did not respond to the treatment and complete remission was obtained only in 3 patients from the group B. On the other hand, 7 patients progressed into CFR. Among them, 5 out of 7 patients with NS (4 from the group A) needed dialysis treatment or doubled their Pcr after a mean of 38 months. This study confirms poor outcome of immunosuppressive treatment in patients with FSGS. However, of the two forms of treatment used in the study, the response appeared to be better and more lasting with cyclophosphamide than with azathioprine and chlorambucil. Corticosteroids associated with pulse cyclophosphamide therapy seems to improve the chances of remission and to protect from renal dysfunction.


Assuntos
Glomerulosclerose Segmentar e Focal/tratamento farmacológico , Imunossupressores/uso terapêutico , Adulto , Anti-Inflamatórios/uso terapêutico , Quimioterapia Combinada , Feminino , Glomerulosclerose Segmentar e Focal/complicações , Humanos , Falência Renal Crônica/etiologia , Masculino , Estudos Retrospectivos , Esteroides
9.
Pol J Pathol ; 49(3): 165-74, 1998.
Artigo em Inglês | MEDLINE | ID: mdl-9810176

RESUMO

Focal segmental glomerulosclerosis (FSGS) poses a major problem both from the clinical and pathomorphological viewpoint. The diagnosis of FSGS in its early stage is vital mainly because of rapidly developing therapeutic modalities. In the literature various changes are discussed which may be of prognostic value (may predict the development of FSGS). One the these changes in vacuolization, mainly of podocytes and less frequently of endothelial cells. The purpose of the present study was to analyse biopsy specimens to find out to what extent vacuolization of podocytes and endothelial cells is associated with FSGS. We compared vacuolization in minimal change disease (MCD), mesangial glomerulonephritis (GNMES) and FSGS. A similar analysis was made also with respect to those cases of MCD and GNMES, in which electron microscopy suggested an early stage of FSGS. In each group electron micrographs obtained from 15 children were analysed. Electron micrographs (12-15 on average) were obtained most frequently from 3 glomeruli. Each case electron micrographs contained 90-100 podocytes. Based upon the same electron micrographs we counted capillary lumina and defined the percentage of those which contained vacuolized endothelia (we counted the capillary lumina, and not the cells, because it is most frequently impossible to identify the border of vacuolized endothelial cells). The number of capillary cross-sections was 60 on average. The results of the analysis were compared with the clinical data. This comparison did not confirm the hypothesis of other investigators that vacuolization is of a prognostic value. Additionally we evaluated the character of vacuoles. Within podocytes the vacuoles were varying in shape. Surrounded by a single membranous layer most frequently they contained material corresponding to proteins or proteoglycans, rarely to lipids. Sometimes the vacuoles were autophagosomal, occasionally they consisted of the dilated rough endoplasmic reticulum. Vacuole-like changes within the capillary lumina were related to the swelling of endothelial cytoplasm or mesangial processes. The reasons for a discrepancy between our results and those reported by other investigators necessitate further studies.


Assuntos
Endotélio Vascular/patologia , Glomerulosclerose Segmentar e Focal/patologia , Glomérulos Renais/citologia , Vacúolos/ultraestrutura , Criança , Humanos , Prognóstico
10.
Pol J Pathol ; 47(4): 215-24, 1996.
Artigo em Inglês | MEDLINE | ID: mdl-9097715

RESUMO

Clinical and morphological studies were carried out in 34 children with the diagnosis of primary membrano-proliferative glomerulonephritis. The studies were prompted by an increasing incidence of this type of glomerulonephritis in Poland in the light of its regular decrease in the world. We found: (a) a number of discrepancies in the clinical manifestations and course of the disease in our children as compared with those in the literature; (b) difficulties in morphological diagnosis; (c) a frequent lack of correlation between the severity of morphological changes and the clinical course. It is of note that our series includes a large group of children below 6 years of age, which is a rare phenomenon.


Assuntos
Glomerulonefrite Membranoproliferativa/patologia , Adolescente , Criança , Pré-Escolar , Feminino , Glomerulonefrite Membranoproliferativa/epidemiologia , Humanos , Incidência , Masculino , Polônia/epidemiologia , Prognóstico
11.
Pol J Pathol ; 45(3): 187-93, 1994.
Artigo em Inglês | MEDLINE | ID: mdl-7953435

RESUMO

Morphometric analysis was used to evaluate mesangial components in MCD (minimal changes disease), GNMes (mesangial glomerulonephritis), FSG (focal segmental glomerulosclerosis). In GNMes the increase of matrix was found to be generally proportional to the amount of mesangial cells. There are clear statistically significant differences in the ratio of matrix volume to cell component volume and of matrix volume to the whole mesangial area in MCD and GNMes as compared with FSG. In the case of GNMes where morphometric results resemble those in FSG cautious prognosis is recommended as there is a possibility of FSG. This has been confirmed both by the course of the disease and the results of repeated biopsy.


Assuntos
Mesângio Glomerular/patologia , Glomerulonefrite/patologia , Nefrose Lipoide/patologia , Adulto , Biópsia , Criança , Humanos , Microscopia Eletrônica , Análise de Regressão
12.
Pol J Pathol ; 45(4): 291-8, 1994.
Artigo em Inglês | MEDLINE | ID: mdl-7697332

RESUMO

We analysed the results of clinical and morphological examinations in 256 children with mesangial glomerulonephritis. In the majority of children the onset of the disease was associated with the nephrotic syndrome, less frequently with erythrocyturia, proteinuria plus erythrocyturia or proteinuria. The course of the disease was markedly worse in those with proteinuria. The patients in this group were most frequently submitted for repeated biopsy. Renal specimens were studied in light, electron and immunofluorescence microscopy. Certain relationships between the initial symptoms and composition of deposits were found. There was, however no relation between the presence or absence of the deposits or their composition and the course of the disease. In 30 children the results of first biopsy (mainly electron microscopy examinations) suggested a possibility of focal segmental glomerulosclerosis (FSG). In 11 out of these children biopsy was repeated confirming the results of the first one. Repeated biopsy was performed in a total of 22 children. Progression of changes (signs of FSG) was found in 19 children, in 2 children changes in repeated biopsy did not differ from those in first biopsy, and in one child regression was observed.


Assuntos
Mesângio Glomerular/patologia , Glomerulonefrite/patologia , Adolescente , Biópsia , Criança , Pré-Escolar , Feminino , Seguimentos , Glomerulosclerose Segmentar e Focal/patologia , Humanos , Lactente , Masculino
14.
Patol Pol ; 43(2): 37-42, 1992.
Artigo em Inglês | MEDLINE | ID: mdl-1296173

RESUMO

Clinicomorphological analysis has been performed in Schoenlein-Henoch nephropathy. Various clinical symptoms are accompanied by morphological changes of variable type and severity. Electron microscopy is a major tool for evaluating these changes. It relatively frequently modifies the diagnosis made by light microscopy. It mainly concerns class I and VI changes (according to a grading system of the International Study Group of Kidney Diseases in Childhood). It was shown that late prognosis was largely determined by the type and severity of morphological changes. Varying severity of changes in individual glomeruli in the same specimen requires in each case a comparison of results obtained by electron microscopy with those obtained by light microscopy in semithin sections. In three children biopsy was repeated. Progression of morphological changes was found in one child. He developed renal failure. In one child morphological changes on first biopsy did not differ from those on second biopsy. Repeated biopsy was performed due to the presence of hypertension. In one child with persistent proteinuria repeated biopsy showed marked attenuation of morphological changes.


Assuntos
Vasculite por IgA/patologia , Nefropatias/patologia , Adolescente , Biópsia , Criança , Pré-Escolar , Feminino , Humanos , Vasculite por IgA/complicações , Nefropatias/etiologia , Glomérulos Renais/ultraestrutura , Alça do Néfron/ultraestrutura , Masculino , Prognóstico
15.
Patol Pol ; 43(2): 43-4, 1992.
Artigo em Inglês | MEDLINE | ID: mdl-1296174

RESUMO

The results of the studies in five children with the syndrome of thin basement membranes have been discussed. A comparison of clinical data with the morphological ones shows that recognition of erythrocyturia or hematuria necessitates familial examinations and long-term follow-up. Final diagnosis of this syndrome may be made only by means of electron microscopy.


Assuntos
Membrana Basal/ultraestrutura , Hematúria/etiologia , Nefropatias/complicações , Criança , Família , Feminino , Humanos , Nefropatias/patologia , Glomérulos Renais/ultraestrutura , Masculino , Proteinúria/etiologia
16.
Patol Pol ; 43(2): 45-50, 1992.
Artigo em Inglês | MEDLINE | ID: mdl-1296175

RESUMO

Clinical and morphological examinations in 25 children with the diagnosis of focal segmental glomerulosclerosis (FSG) show that: 1. electron microscopy permits a detection of FSG in its early stage when light microscopy does not reveal any changes, and immunomorphological studies do not show deposits 2. in this stage small focal interstitial changes may be visible 3. ultrastructural studies reveal changes in case of nonspecific light microscopy i.e. suggesting mesangial glomerulonephritis 4. in doubtful cases it is useful to repeat biopsy.


Assuntos
Glomerulosclerose Segmentar e Focal/patologia , Adolescente , Biópsia , Criança , Pré-Escolar , Feminino , Seguimentos , Humanos , Glomérulos Renais/ultraestrutura , Masculino
20.
Zentralbl Chir ; 109(5): 309-18, 1984.
Artigo em Alemão | MEDLINE | ID: mdl-6720125

RESUMO

The paper presents the author's experiments performed for examining the morphological adaptation in the intestinal loop, preserved for the food passage after jejunoileostomy for extreme obesity. Macro-, micro-, and ultramicroscopic examinations were carried out in 20 patients. A characteristic elongation and hypertrophy of the active intestinal loop, thickening of its wall (especially mucosa), hypertrophy and elongation of the intestinal villi as well as reduction of the mucous cells could be observed. The enzymatic activity of the mucous membrane could be found to be increased in the active part of the jejunum too. In the author's opinion the observed changes clearly demonstrate the transient character of those metabolic disturbances which develop after producing the artificial short gut syndrome.


Assuntos
Adaptação Fisiológica , Íleo/cirurgia , Intestino Delgado/fisiologia , Jejuno/cirurgia , Obesidade/terapia , Adulto , Feminino , Humanos , Hipertrofia , Mucosa Intestinal/enzimologia , Intestino Delgado/enzimologia , Intestino Delgado/patologia , Masculino , Pessoa de Meia-Idade
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