Corneal stromal localization of TGF beta isoforms in spontaneous persistent epithelial defects after PRK in rabbits.

The purpose of this study was to evaluate transforming growth factor beta (TGFß) isoform localization in rabbit corneas with spontaneous persistent epithelial defects (PEDs) after photorefractive keratectomy (PRK). Four cryofixed corneas from a previously reported series of PEDs in rabbits that had PRK were evaluated with triplex immunohistochemistry (IHC) for TGFß3, myofibroblast marker alpha-smooth muscle actin (α-SMA) and mesenchymal marker vimentin. One cornea had sufficient remaining tissue for triplex IHC for TGFß1, TGFß2, or TGFß3 (each with α-SMA and vimentin) using isoform-specific antibodies. All three TGFß isoforms were detected in the subepithelial stroma at and surrounding the PED. Some of each TGFß isoform co-localized with α-SMA of myofibroblasts, which could be TGFß isoform autocrine production by myofibroblasts or TGFß-1, -2, and -3 binding to these myofibroblasts.

Early LASIK flap displacement without signs of infection.

A 37-year-old woman was referred for refractive surgery evaluation. Poor visual quality in her left eye is her chief concern. The patient had undergone laser in situ keratomileusis (LASIK) in both eyes 3 days previously. Detailed history revealed that the patient underwent surgery to correct low myopia and astigmatism (-2.50 -2.75 × 180 in the right eye and -1.25 -2.75 × 180 in the left eye). The preoperative evaluation corrected distance visual acuity (CDVA) was 20/20 in the right eye and 20/20 in the left eye. The surgery was performed with a mechanical microkeratome and was uneventful. The patient reports that after her vision improved on the first day, she woke up with blurry vision in her left eye on the second day and it remained that way until the third day when she sought medical help. The slitlamp examination showed LASIK flap displacement without signs of infection ( Figure 1JOURNAL/jcrs/04.03/02158034-202212000-00023/figure1/v/2022-12-01T092452Z/r/image-tiff ). The patient was using topical corticosteroids and topical antibiotics every 6 hours. Considering the likely stromal exposure time, what would be the best approach for this case? Does the probable time of displacement of this flap change its behavior? In what way? If you decide to reposition the flap, would it be interesting to use fibrin glue or suture? Considering that there was no major trauma in the patient's report, what is the importance of the LASIK flap having been created by a mechanical microkeratome and not by a femtosecond laser in the displacement of the flap?