Development and physiochemical assessment of graphene-bioactive glass-P(3HB-co-4HB) composite scaffold as prospect biomaterial for wound healing.

The clinical management of wounds presents a considerable challenge because dressing selection must prioritise the provision of appropriate barrier and the healing properties, consider patient's compliance factors such as comfort, functionality and practicality. This study primarily aimed to develop a composite scaffold patch for potential application in wound healing. Poly(3-hydroxybutyrate-co-4-hydroxybutyrate) [P(3HB-co-4HB)] is a biopolymer that originated from bacteria. It is well-recognised owing to its distinctive mechanical and physical characteristics suitable for biomedical applications. Graphene (G) and bioactive glass (BG) are biocompatible towards humans, and enhanced properties are achievable by adding biopolymer. In this study, composite scaffolds were developed by combining P(3HB-co-4HB) at a distinct proportion of 4HB monomer reinforced with G (3.0 wt.%) and BG (2.5 wt.%) by using solvent casting, resulting in two types of composite scaffolds: P(3HB-co-25%4HB)/G/BG and P(3HB-co-37%4HB)/G/BG. A successful composite scaffold as a unified structure was achieved based on chemical assessments of organic and inorganic elements within the composites. The pure polymer displayed a smooth surface, and the BG and G addition into the composite scaffolds increased surface roughness, forming irregular pores and protuberances. The wettability and hydrophilicity of the composites significantly improved up to 40% in terms of water uptake. An increment in crystallisation temperature diminished the flexibility of the composite's scaffolds. Evaluation of Presto Blue biocompatibility demonstrated nontoxic behaviour with a dosage of less than 25.00 mg ml-1of composite scaffold-conditioned media. The L929 fibroblast cells displayed excellent adhesion to both types of composite scaffolds, as evidenced by the increased percentage of cell viability observed throughout 14 d of exposure. These findings demonstrate the importance of optimising each component within the composite scaffolds and their interrelation, paving the way for excellent material properties and enhancing the potential for wound healing applications.

Biocompatible Nutmeg Oil-Loaded Nanoemulsion as Phyto-Repellent.

Plant essential oils are widely used in perfumes and insect repellent products. However, due to the high volatility of the constituents in essential oils, their efficacy as a repellent product is less effective than that of synthetic compounds. Using a nanoemulsion as a carrier is one way to overcome this disadvantage of essential oils. Nutmeg oil-loaded nanoemulsion (NT) was prepared using a high speed homogenizer and sonicator with varying amounts of surfactant, glycerol, and distilled water. Using a phase diagram, different formulations were tested for their droplet size and insect repellent activity. The nanoemulsion containing 6.25% surfactant and 91.25% glycerol (NT 6) had the highest percentage of protection (87.81%) in terms of repellent activity among the formulations tested for the 8 h duration of the experiment. The droplet size of NT 6 was 217.4 nm, and its polydispersity index (PDI) was 0.248. The zeta potential value was -44.2 mV, and the viscosity was 2.49 Pa.s at pH 5.6. The in vitro release profile was 71.5%. When the cytotoxicity of NT 6 at 400 µg/mL was tested using the MTS assay, cell viability was 97.38%. Physical appearance and stability of the nanoemulsion improved with the addition of glycerol as a co-solvent. In summary, a nutmeg oil-loaded nanoemulsion was successfully formulated and its controlled release of the essential oil showed mosquito repellent activity, thus eliminating the disadvantages of essential oils.