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Background and Aim: The feeding and sanitary conditions significantly influence the productivity of farm animals. This study aimed to assess the impact of a lactic acid-producing microbial additive on broiler chicken productivity. Materials and Methods: A 42-day experimental period utilized 120 1-day-old Cobb 500 chicks with an average weight of 46 g. In groups of 30 each, the chicks were randomly assigned to four experimental designs. The following treatments were assessed: T1 without intervention (control), T2 with bacitracin at a concentration of 0.5 g/L, T3 with a 5% probiotic mixture (PM), and T4 with a 7.5% PM. The birds were fed the commercial balanced feed without anticoccidials daily, while vaccines were administered according to the recommended biosecurity plan by the commercial house. Drinking water was treated with PM containing lactobacilli, yeasts, and short-chain organic acids. Result: In T4, a 7.5% PM resulted in a final weight of 2361.2 g (p < 0.05), a total weight gain of 1412.8 g (p < 0.05), and improved feed efficiency with a feed conversion of 2.00 (p < 0.05), during which feed intake was lower than in the other groups. Conclusion: Microbial additives with lactic acid activity are a cost-effective and feasible solution for broiler chicken productivity.
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Transition metal-doped ceria (M-CeO2) catalysts (M = Fe, Co, Ni and Cu) with multiple loadings were experimentally investigated for reverse water gas shift (RWGS) reaction. Density functional theory (DFT) calculations were performed to benchmark the properties that impact catalytic activity of CO2 reduction. Temperature-programmed desorption (TPD) was conducted to study the CO2 binding strength on doped CeO2 surfaces; the trend of the energy along increasing metal loading agrees with the DFT calculations. Notably, CO2 dissociative adsorption energy and oxygen vacancy (OV) formation energy are key descriptors obtained from both DFT and experiments, which can be used to evaluate catalytic performance. Results show the effectiveness of transition metal doping in enhancing CO2 adsorption and reducibility of the surfaces, with Fe showing particularly promising results, i.e., CO2 conversion higher than 56% at 600 °C and 100% selectivity to CO. Cu exhibits 100% selectivity to CO but low CO2 conversion, while Co and Ni showed notable ability of methanation, particularly at high loadings. This study finds that an effective CeO2 based RWGS catalyst corresponds to OV sites that have low OV formation energies for surface reduction, and moderate CO2 adsorption energies for strong interaction with the surface to promote C-O bond scission.
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INTRODUCTION: The stigma and social discrimination against cisgender women sex workers lead many of them to live in conditions characterized by social inequality, marginalization, persecution, and limited opportunities for sexual health literacy. Consequently, they are often compelled to establish a framework of preventive beliefs with little scientific validity, which they use to identify, mitigate, or avoid sexual health risks arising from their interactions with clients. This study investigates the sexual health beliefs that influence self-care practices aimed at preventing sexually transmitted infections among cisgender women sex workers in Colombia. METHODS: We conducted a qualitative study framed in Hermeneutic Phenomenology. In-depth interviews and discussion groups were conducted with 34 cisgender women sex workers over 18 years of age in the center of the cities of Bogotá and Barranquilla in Colombia. RESULTS: Seven themes emerged from the reflective and inductive thematic analysis of the narratives: (1) popular habits for the prevention of contagion, (2) ocular assessment of genitals, (3) condom lubrication, (4) suspicion of a hidden infection in the client, (5) saliva and oral contact as a source of contagion, (6) avoidance of semen contact, (7) and trust in God as protection. CONCLUSIONS: The findings reflect the need for health and social professionals to promote participatory and inclusive cooperation with sex workers to update the framework of preventive beliefs that help them guide sexual health self-care with autonomy and self-efficacy, strengthening favorable beliefs and negotiating unfavorable ones. It is also essential to have a sex worker-informed sexual health policy that guides the promotion of sexual health that is sensitive to the needs and consistent with the risks of sex work and ensures friendly and non-oppressive preventive care environments for sex workers.
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Profissionais do Sexo , Saúde Sexual , Infecções Sexualmente Transmissíveis , Humanos , Feminino , Colômbia/epidemiologia , Infecções Sexualmente Transmissíveis/prevenção & controle , Infecções Sexualmente Transmissíveis/epidemiologia , Profissionais do Sexo/psicologia , Profissionais do Sexo/estatística & dados numéricos , Adulto , Conhecimentos, Atitudes e Prática em Saúde , Adulto Jovem , Pesquisa Qualitativa , Pessoa de Meia-Idade , Comportamento Sexual/psicologiaRESUMO
C. berlandieri ssp. berlandieri (C. berlandieri) is one of the most common members of the group of plants known as quelites, which are dark leafy greens widely consumed in Mexico. This study aimed to evaluate the impact of two drying procedures (oven drying and freeze-drying/lyophilization) on the polyphenolic composition, antioxidant capacity, and proximal chemical analysis of C. berlandieri leaves and inflorescences (raw or boiled). The results indicated that the raw freeze-dried samples had higher amounts (p < 0.05) of total phenolic compounds, total flavonoids, and antioxidant capacity, mainly in the inflorescence. The oven-dried samples showed an increased concentration of polyphenols after boiling, while the lyophilized samples showed a slightly decreased concentration. The drying process was observed to have little impact on the proximal chemical composition. Quantification by UPLC-DAD-ESI-QToF/MS identified up to 23 individual phenolic compounds, with freeze-dried samples showing higher amounts of individual compounds compared with oven-dried. Procyanidin B2 was found exclusively in the inflorescences. The inflorescences have a higher content of phenolic compounds and greater antioxidant capacity than the leaves. Regardless of the drying process, the leaves and inflorescences of C. berlandieri contain an interesting variety of phenolic compounds that may have beneficial effects on health.
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Antioxidantes , Inflorescência , Antioxidantes/química , Inflorescência/química , Dessecação/métodos , Fenóis/química , LiofilizaçãoRESUMO
PREMISE: Alismataceae, a sub-cosmopolitan family with ca. 17 genera and 113 species, is a large group of aquatic plants. Compression/impressions and bioinclusions of reproductive parts in amber support the documentation of the lineage in low-latitude North America. In Mexico, fossil aquatic plants have been infrequently documented. The new reproductive structures exhibit characteristics of Alismataceae, whose fossil record is mainly documented in the northern hemisphere through of fruits and seeds. METHODS: We described and compared 150 samples of reproductive structures preserved as impressions/compressions from the Oligocene Los Ahuehuetes locality in the state of Puebla, and two bioinclusions from the Miocene amber of Simojovel de Allende in the state of Chiapas, Mexico with extinct and extant taxa. Using a parsimony analysis based on 29 floral characters of 17 extant genera of the Alismataceae, we evaluated the relationship between the fossil material and potential living relatives. RESULTS: We discovered a new genus Nichima based on a perfect, actinomorphic flower with an expanded receptacle, three persistent sepals with multiple vasculatures, delicate and caducous petals, six stamens, and a gynoecium composed of three to more superior carpels, maturing into achenes. These characteristics resemble flowers of Alismataceae. Nichima represents an extinct member of the family, with two new species described here, Nichima magalloniae L. Hern., Cevallos-Ferriz et Hernández-Damián sp. nov. and Nichima gonzalez-medranoi L. Hern., Cevallos-Ferriz et Hernández-Damián, sp. nov. Their phylogenetic position suggests affinity with a clade that includes Baldiella, Echinodorus, and Alisma. CONCLUSIONS: Reproductive structures from the Cenozoic of Mexico support the identification of a new extinct genus, Nichima, evidencing the extensive history of Alismataceae in North America's low latitudes and suggesting a southern extension of the boreotropical flora.
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Alismataceae , Filogenia , México , Âmbar , Flores , FósseisRESUMO
This work aimed to study the relationship between the perception of organizational democracy and gender discrimination at a Chilean public university. It is known that organizational democracy is not only about organizational life but also about democratic perceptions, attitudes, and behaviors in social life, as found in academic contexts. The methodology used factor analysis and descriptive and inferential statistical techniques to analyze data from a survey administered to 704 university faculty members, with a response rate of 58.1%. The gender distribution of this respondent population was 67% male and 37% female, values equivalent to the Chilean public university system (60% and 40%, respectively). The results highlight the importance of gender perspective in higher education. Indeed, academics who perceive greater gender discrimination toward women appreciate the deployment of organizational democracy to a lesser extent. Moreover, a high perception of discrimination on the part of women is confirmed (46%), them being, in turn, the ones who show a greater predisposition toward gender equality. This research intends to contribute to the development of strategies to remove obstacles to gender equality and improve the commitment of the academic community to institutional progress.
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Urban bird dwellers survive and reproduce in highly urbanized ecosystems. Some individuals adjust to these novel conditions by changing natural nesting materials for artificial materials, thus making nests more conspicuous in the environment. The consequences of using artificial materials for nesting remain poorly understood, especially from a nest-predator perspective. We studied if exposed artificial materials on bird nests affect the daily survival rate in a common dweller species, the clay-colored thrush (Turdus grayi). We used previously collected nests with a different area of exposed artificial materials and placed them with clay eggs on the main campus of the Universidad de Costa Rica. During 12 days of the reproductive season, we monitored the nests using trap cameras located in front of each nest. We found that nest survival decreased as the proportion of exposed artificial materials increased in the nest and, unexpectedly, that the principal predators were conspecifics. Thus, artificial materials used in the outer layer of nests make them more susceptible to predation. The use of artificial materials likely reduce reproductive success and population size in urban clay-colored thrush, but further field experiments measuring the effect of waste in birds' nests on the reproductive success of urban birds are needed.
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Ecossistema , Aves Canoras , Humanos , Animais , Argila , Comportamento de Nidação , ReproduçãoRESUMO
After establishing secondary contact, recently diverged populations may remain reproductively isolated or may hybridize to a varying extent depending on factors such as hybrid fitness and the strength of assortative mating. Here, we used genomic and phenotypic data from three independent contact zones between subspecies of the variable seedeater (Sporophila corvina) to examine how coloration and genetic divergence shape patterns of hybridization. We found that differences in plumage coloration are probably maintained by divergent selection across contact zones; however, the degree of plumage differentiation does not match overall patterns of hybridization. Across two parallel contact zones between populations with divergent phenotypes (entirely black vs. pied plumage), populations hybridized extensively across one contact zone but not the other, suggesting that plumage divergence is not sufficient to maintain reproductive isolation. Where subspecies hybridized, hybrid zones were wide and formed by later-generation hybrids, suggesting frequent reproduction and high survivorship for hybrid individuals. Moreover, contemporary gene flow has played an important role in shaping patterns of genetic structure between populations. Replicated contact zones between hybridizing taxa offer a unique opportunity to explore how different factors interact to shape patterns of hybridization. Overall, our results demonstrate that divergence in plumage coloration is important in reducing gene flow but insufficient in maintaining reproductive isolation in this clade, and that other factors such as divergence in song and time since secondary contact may also play an important role in driving patterns of reduced hybridization and gene flow.
Al establecer contacto secundario, las poblaciones que divergieron recientemente pueden permanecer reproductivamente aisladas o pueden hibridarse en distintos grados, dependiendo de factores como la aptitud (fitness) y la fuerza del apareamiento selectivo. Aquí, utilizamos datos genómicos y fenotípicos de tres zonas de contacto independientes entre subespecies del Semillero Variable (Sporophila corvina), para examinar cómo la coloración y la divergencia genética regulan los patrones de hibridación. A través de las zonas de contacto, encontramos que las diferencias en la coloración del plumaje posiblemente se mantienen por selección divergente, pero el grado de diferenciación no coincide con los patrones generales de hibridación. En dos zonas de contacto análogas entre poblaciones con fenotipos divergentes (totalmente negro vs plumaje de varios colores), las poblaciones hibridaron ampliamente en una zona de contacto, pero no en la otra, lo que sugiere que la divergencia del plumaje no es suficiente para mantener el aislamiento reproductivo. Donde las subespecies hibridaron, las zonas híbridas eran amplias y estaban formadas por híbridos de generaciones posteriores, lo que sugiere reproducción frecuente y alta sobrevivencia de los híbridos. Además, el flujo génico ha desempeñado un papel importante en la configuración de patrones de estructura genética entre poblaciones. Las réplicas de zonas de contacto entre taxones que hibridan ofrecen una oportunidad para explorar cómo interactúan diversos factores para dar forma a los patrones de hibridación. En general, nuestros resultados demuestran que la divergencia en la coloración del plumaje es importante para reducir el flujo génico, pero insuficiente para mantener el aislamiento reproductivo en este clado, y que otros factores, como la divergencia en el canto y el tiempo transcurrido desde el contacto secundario, también pueden desempeñar un papel importante en la reducción del flujo génico e hibridación.
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Passeriformes , Isolamento Reprodutivo , Animais , Passeriformes/genética , Deriva Genética , Hibridização Genética , Fluxo GênicoRESUMO
Abstract Beaucarnea inermis is an endemic species from Northeast Mexico, in the states of San Luis Potosí and Tamaulipas. It is appreciated as an ornamental plant, so its populations are subject to the poaching of individuals for illegal trade. Previous studies determined that their populations have been affected due to the disturbance since the incidence of anthropogenic activities affects the viability of the species. Here we determine the current conservation status of B. inermis and identify their main risk factor by performing an extinction risk assessment based on the Annex II "Method for Evaluation of Risk of Extinction of Plants in Mexico". We studied 10 populations of B. inermis from protected and non-protected areas in San Luis Potosí and Tamaulipas. We considered the MER criteria: A) geographical distribution characteristics, B) habitat characteristics, C) intrinsic biological vulnerability, and D) impact of human activity. Using field and analyzed data, the MER assessment gives 1.91 points that confirm B. inermis is correctly classified as an Endangered species. The natural protected areas where the species occurs represent cores for its protection; however, the surface of these areas may not be sufficient without biological corridors that connect them.
Resumen Beaucarnea inermis es una especie endémica del Noroeste de México distribuida en los estados de San Luis Potosí y Tamaulipas. Es apreciada como planta ornamental, por lo que sus poblaciones están sujetas al saqueo de individuos para su comercialización ilegal. En trabajos anteriores se determinó que sus poblaciones han sido afectadas debido al disturbio por la incidencia de actividades antrópicas que afectan su viabilidad. En la presente contribución se determina el nivel de riesgo de B. inermis con base en el Anexo Normativo II "Método de Evaluación de Riesgo de Extinción de Plantas en México". Se estudiaron 10 poblaciones de B. inermis en San Luis Potosí y Tamaulipas, tanto en áreas naturales protegidas como en sitios no protegidos. Los criterios MER considerados fueron: A) características de la distribución geográfica, B) características del hábitat, C) vulnerabilidad biológica intrínseca y D) impacto de la actividad humana. El análisis MER arroja un valor de 1.91 que, confirma a B. inermis dentro de la categoría de Amenazada. Las áreas naturales protegidas donde se distribuye la especie funcionan como núcleos de protección, sin embargo, su superficie puede no ser suficiente sin la presencia de corredores biológicos que las conecten.
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Heterologous gene activation causes non-physiological burden on cellular resources that cells are unable to adjust to. Here, we introduce a feedforward controller that actuates growth rate upon activation of a gene of interest (GOI) to compensate for such a burden. The controller achieves this by activating a modified SpoT enzyme (SpoTH) with sole hydrolysis activity, which lowers ppGpp level and thus increases growth rate. An inducible RelA+ expression cassette further allows to precisely set the basal level of ppGpp, and thus nominal growth rate, in any bacterial strain. Without the controller, activation of the GOI decreased growth rate by more than 50%. With the controller, we could activate the GOI to the same level without growth rate defect. A cell strain armed with the controller in co-culture enabled persistent population-level activation of a GOI, which could not be achieved by a strain devoid of the controller. The feedforward controller is a tunable, modular, and portable tool that allows dynamic gene activation without growth rate defects for bacterial synthetic biology applications.
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Guanosina Tetrafosfato , Biologia Sintética , Guanosina Tetrafosfato/metabolismo , Ativação TranscricionalRESUMO
BACKGROUND: Schizophrenia and related disorders are highly disabling and create substantial burdens for families, communities, and health care systems. Although pharmacological treatments can often lessen the psychotic symptoms that are a hallmark of schizophrenia, they do not lessen the social and cognitive deficits that create the greatest impediments to community engagement and functional recovery. This study builds on prior research on psychosocial rehabilitation by comparing the effectiveness of two treatments demonstrated as efficacious in improving social and community functioning, Cognitive Enhancement Therapy (CET) and a version of Social Skills Training (HOPES/SST). METHODS: The study uses a randomized cluster design in which a pair of clinicians at community- and hospital-based mental service centers deliver either CET or HOPES to at least one group of 6-8 eligible clients for 12 months. Clinicians are trained and then supervised weekly, with ongoing process measurement of treatment fidelity, attendance, satisfaction, and retention, and use of other services. Measures administered at baseline and at 6 and 12 months while in treatment, and then at 18 and 24 months after treatment include social adjustment, quality of life, social skills, positive and negative symptoms, and neuro- and social cognition. We hypothesize that CET will be associated with greater improvements than SST in both the primary outcome of community functioning and the secondary outcomes of neuro- and social cognition and social skills. Secondarily, we hypothesize that more cognitive impairment at baseline and younger age will predict more benefit from CET compared to HOPES. DISCUSSION: Resource shortages endemic in mental health services and exacerbated by the pandemic highlight the importance of identifying the most effective approach to improving social and community functioning. We aim to improve understanding of the impact of two efficacious psychosocial treatments and to improve clinicians' ability to refer to both treatments the individuals who are most likely to benefit from them. We expect the result to be programmatic improvements that improve the magnitude and durability of gains in community functioning. TRIAL REGISTRATION: ClinicalTrial.gov NCT04321759 , registered March 25, 2020.
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Terapia Cognitivo-Comportamental , Esquizofrenia , Cognição , Humanos , Qualidade de Vida , Esquizofrenia/diagnóstico , Habilidades Sociais , Resultado do TratamentoRESUMO
AbstractMany animals respond to heterospecific signals that indicate the presence of food or predators. Although the benefits of responding are clear, the behavioral and cognitive mechanisms underlying responses are not. Whether responses are learned, innate, or an epiphenomenon created by following other species as they respond to signals remains unknown because most studies have involved respondents that are sympatric with their heterospecific signalers and that have therefore had opportunities to learn their signals. In this study, we tested the mechanisms underlying avian responses to heterospecific chick-a-dee calls. All North American parids produce chick-a-dee calls in response to arousing stimuli, such as food and predators, and diverse species respond by approaching the caller and consuming the food or mobbing the predator. We broadcast chick-a-dee calls plus two control stimuli in Costa Rica, Colombia, and Brazil, where no parids ever occur. We conducted our trials in the winter, when Neotropical migrants that might be familiar with chick-a-dee calls were present, and in the temperate breeding season, when migrants were absent. Across 138 trials, 38 resident species from 14 families and four orders responded to chick-a-dee calls by approaching to within 5 m of the playback speaker. A phylogenetic logistic regression showed that whether a species responded was not significantly associated with the species' mean body mass or the structural similarity between its calls and chick-a-dee calls. Residents were significantly more likely to approach chick-a-dee calls than either control stimulus. This pattern was unaffected by the presence of migrants, thus demonstrating that the observed responses are innate. Our study shows that learning cannot fully explain responses to heterospecific chick-a-dee calls and that structural features distinguishing these calls from other vocalizations are important.
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Aves Canoras , Vocalização Animal , Acústica , Animais , Aprendizagem , Filogenia , Aves Canoras/fisiologia , Vocalização Animal/fisiologiaRESUMO
CO2 electroreduction reaction offers an attractive approach to global carbon neutrality. Industrial CO2 electrolysis towards formate requires stepped-up current densities, which is limited by the difficulty of precisely reconciling the competing intermediates (COOH* and HCOO*). Herein, nano-crumples induced Sn-Bi bimetallic interface-rich materials are in situ designed by tailored electrodeposition under CO2 electrolysis conditions, significantly expediting formate production. Compared with Sn-Bi bulk alloy and pure Sn, this Sn-Bi interface pattern delivers optimum upshift of Sn p-band center, accordingly the moderate valence electron depletion, which leads to weakened Sn-C hybridization of competing COOH* and suitable Sn-O hybridization of HCOO*. Superior partial current density up to 140 mA/cm2 for formate is achieved. High Faradaic efficiency (>90%) is maintained at a wide potential window with a durability of 160 h. In this work, we elevate the interface design of highly active and stable materials for efficient CO2 electroreduction.
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Disentangling the evolutionary relationships of rapidly radiating clades is often challenging because of low genetic differentiation and potentially high levels of gene flow among diverging taxa. The genus Sporophila consists of small Neotropical birds that show, in general, relatively low genetic divergence, but particularly high speciation rates and pronounced variation in secondary sexual traits (e.g., plumage color), which can be important in generating premating reproductive isolation. In cases like these, the use of genome-wide sequence data can increase the resolution to uncover a clade's evolutionary history. Here, we used a phylogenomic approach to study the evolutionary history and genetic structure of the Variable Seedeater superspecies complex, which includes S. corvina, S. intermedia, and S. americana. Using â¼25,000 genome-wide single nucleotide polymorphisms (SNPs), we confirmed that the Variable Seedeater superspecies complex is monophyletic. However, a phylogenetic reconstruction based on a mitochondrial marker (ND2) resulted in a discordant tree topology, particularly in the position of Wing-barred Seedeater S. americana, which might be due to a mitochondrial capture event. Our results suggest historical gene flow among lineages, particularly between species with conflicting topologies. Among the four phenotypically variable S. corvina subspecies, our structure analyses identified three main distinct genetic groups (K = 3), and that the entirely black subspecies, S. c. corvina, is derived from within a pied-colored clade. Further, we inferred widespread gene flow across the whole species' distribution, including between subspecies. However, gene flow was about 100 times lower at the geographic boundaries of the entirely black and the pied subspecies, suggesting an important role for plumage divergence in limiting gene flow. Overall, our findings suggest that the early diversification of the Sporophila genus occurred rapidly despite historical gene flow between lineages and that divergence in plumage color possibly influences the extent of gene flow among taxa.
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Fluxo Gênico , Passeriformes , Animais , Evolução Biológica , DNA Mitocondrial/química , DNA Mitocondrial/genética , Passeriformes/genética , FilogeniaRESUMO
Gene flow in birds can be affected by urbanization depending on natural history traits and adaptability to habitat change. Contrasting results can be expected when comparing species with opposite resilience to urbanization. In this study, we assessed genetic diversity and structure for two bird species, the urban avoider white-eared ground-sparrow, Melozone leucotis, and the urban dweller house wren Troglodytes aedon. We used seven microsatellite loci and sampled five locations with differing levels of urbanization in Costa Rica. We found considerably higher genetic structure in white-eared ground-sparrows than in house wrens. Circuit theory analyses proved a higher isolation from urban resistance for the white-eared ground-sparrow than for house wrens. These results support that urbanization is a significant barrier for gene flow in urban avoiders, in contrast to urban dweller species that showed little to no impact. Differences could be attributed to a higher plasticity in habitat and nesting site preferences in the house wren, and significant dispersal limitation for the white-eared ground-sparrow. These results emphasize the need for conservation strategies towards white-eared ground-sparrows and other urban avoider species whose habitat and connectivity have been reduced by the recent urban expansion.
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Pardais , Urbanização , Animais , Ecossistema , Fluxo Gênico , Repetições de Microssatélites/genética , Pardais/genéticaRESUMO
This work presents the development of a new kinetic Monte Carlo algorithm, referred to as Moving Front kinetic Monte Carlo (MFkMC), for simulating processes subject to moving interfaces. This framework is designed to capture the movement of transiently varying interfaces in a kinetic-like manner so that its movement can be described using Monte Carlo sampling. The MFkMC algorithm accomplishes this task by evaluating the behavior of the interfacial molecules and assigning kinetic Monte Carlo-style rate equations that describe the transition probability that a molecule would advance into the neighboring phase, displacing an interfacial molecule from the opposing phase and thus changing the interface. Due to its kinetic Monte Carlo structure, the MFkMC algorithm can additionally account for other important interfacial phenomena, such as interfacial surface reactions. The proposed algorithm was tested via applications to three different simple interfacial case studies. These studies validate the MFkMC algorithm and demonstrate its capabilities to accurately and efficiently simulate a variety of different moving interface systems.
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BACKGROUND: Pseudalcantarea grandis (Schltdl.) Pinzón & Barfuss is a tank bromeliad that grows on cliffs in the southernmost portion of the Chihuahuan desert. Phytotelmata are water bodies formed by plants that function as micro-ecosystems where bacteria, algae, protists, insects, fungi, and some vertebrates can develop. We hypothesized that the bacterial diversity contained in the phytotelma formed in a bromeliad from an arid zone would differ in sites with and without surrounding vegetation. Our study aimed to characterize the bacterial composition and putative metabolic functions in P. grandis phytotelmata collected in vegetated and non-vegetated sites. METHODS: Water from 10 individuals was sampled. Five individuals had abundant surrounding vegetation, and five had little or no vegetation. We extracted DNA and amplified seven hypervariable regions of the 16S gene (V2, V4, V8, V3-6, 7-9). Metabarcoding sequencing was performed on the Ion Torrent PGM platform. Taxonomic identity was assigned by the binning reads and coverage between hit and query from the reference database of at least 90%. Putative metabolic functions of the bacterial families were assigned mainly using the FAPROTAX database. The dominance patterns in each site were visualized with rank/abundance curves using the number of Operational Taxonomic Units (OTUs) per family. A percentage similarity analysis (SIMPER) was used to estimate dissimilarity between the sites. Relationships among bacterial families (identified by the dominance analysis and SIMPER), sites, and their respective putative functions were analyzed with shade plots. RESULTS: A total of 1.5 million useful bacterial sequences were obtained. Sequences were clustered into OTUs, and taxonomic assignment was conducted using BLAST in the Greengenes databases. Bacterial diversity was 23 phyla, 52 classes, 98 orders, 218 families, and 297 genera. Proteobacteria (37%), Actinobacteria (19%), and Firmicutes (15%) comprised the highest percentage (71%). There was a 68.3% similarity between the two sites at family level, with 149 families shared. Aerobic chemoheterotrophy and fermentation were the main metabolic functions in both sites, followed by ureolysis, nitrate reduction, aromatic compound degradation, and nitrogen fixation. The dominant bacteria shared most of the metabolic functions between sites. Some functions were recorded for one site only and were related to families with the lowest OTUs richness. Bacterial diversity in the P. grandis tanks included dominant phyla and families present at low percentage that could be considered part of a rare biosphere. A rare biosphere can form genetic reservoirs, the local abundance of which depends on external abiotic and biotic factors, while their interactions could favor micro-ecosystem resilience and resistance.
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Bromeliaceae , Ecossistema , Animais , Humanos , Bromeliaceae/genética , Filogenia , Bactérias/genética , Água , PlantasRESUMO
The percentage of individuals who are functionally bilingual in the United States has grown substantially in the last 3 to 4 decades. Nevertheless, bilingual mental health providers remain relatively scarce and bilingualism in psychosis or schizophreniaspectrum disorders remains relatively unexplored. Here, we present a clinical case study of a man with schizophrenia who presented his psychotic symptoms differently in his primary and secondary languages. We also consider this case in the context of other published cases with similar themes. Based on our review, we hypothesize that the presentation of psychotic symptoms may be influenced by the language a person uses, and more specifically, by their cognitive abilities to speak that language and/or their emotional attachment to that language. We outline the importance of obtaining a thorough language background of each patient with psychosis and investigate the ways in which a second language could serve as a protective factor against functional decline in psychotic and healthy populations. We suggest that attempts to engage bilingual patients with psychosis clinically in each language could lead to a more holistic evaluation of psychotic and disorganized symptoms and thus lead to more multidimensional intervention strategies.
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Multilinguismo , Transtornos Psicóticos , Esquizofrenia , Masculino , Humanos , Idioma , Transtornos Psicóticos/complicações , Transtornos Psicóticos/diagnóstico , Esquizofrenia/complicações , CogniçãoRESUMO
OBJECTIVE: Cognitive enhancement therapy (CET) is an 18-month comprehensive cognitive remediation intervention designed to improve cognition and functioning among patients with schizophrenia. The current study sought to confirm previously observed benefits of CET on cognitive and behavioral outcomes in the early course of the condition in a large multisite trial. METHODS: Overall, 102 outpatients with early-course schizophrenia were randomly assigned to 18 months of CET (N=58) or enriched supportive therapy (EST; N=44). Participants completed cognitive, social adjustment, and symptom assessments at baseline and at 9 and 18 months. Composite indices were calculated for these outcomes. Mixed-effects models investigated differential changes in outcomes between CET and EST. Because of a high attrition rate, sensitivity analyses of data from treatment completers (N=49) were conducted. RESULTS: The effects of CET on improved overall cognition were confirmed and tentatively confirmed for social cognition in both intent-to-treat and completer analyses, and beneficial effects on attention/vigilance were also observed. An effect of CET on social adjustment was not confirmed in the analyses, because both CET and EST groups had considerably improved social adjustment. Although not statistically significant, the between-group effect size for CET's effect on social adjustment doubled from the intent-to-treat (Cohen's d=0.23) to completer analyses (Cohen's d=0.51) (p=0.057). Both groups displayed similar symptom improvements. CONCLUSIONS: CET effectively improved cognition among patients with early-course schizophrenia. The functional benefits of CET appeared to increase with treatment retention. Further research is needed to understand predictors of attrition and mechanisms of change during CET for this population.