RESUMO
The depressed natural killer (NK) activity, anti-body-dependent cellular cytotoxicity (ADCC) and NK cytotoxic factor cytotoxicity in untreated non-Hodgkin's lymphoma patients were found to be elevated after chemotherapy. In vitro treatment of the effector NK cells with interferon alpha could augment the NK activity in normal subjects and treated patients to a comparable degree. Chemotherapy mainly affected the post-binding events in the NK cytotoxic process by causing an increase in the active killing potential of the NK cells. This study provides a better understanding of changes in the NK cytotoxic mechanism in non-Hodgkin's lymphoma patients and the role of interferon in this process.
Assuntos
Citotoxicidade Celular Dependente de Anticorpos , Células Matadoras Naturais/imunologia , Linfoma não Hodgkin/tratamento farmacológico , Adjuvantes Imunológicos/farmacologia , Citotoxicidade Celular Dependente de Anticorpos/efeitos dos fármacos , Testes Imunológicos de Citotoxicidade , Humanos , Imunidade Inata/efeitos dos fármacos , Interferon Tipo I/farmacologia , Células Matadoras Naturais/efeitos dos fármacos , Fatores Matadores de Levedura , Linfoma não Hodgkin/imunologia , Proteínas/fisiologiaAssuntos
Concanavalina A/farmacologia , Ativação Linfocitária/efeitos dos fármacos , Linfócitos T Reguladores/efeitos dos fármacos , Neoplasias do Colo do Útero/imunologia , Anticorpos Monoclonais/imunologia , Feminino , Humanos , Fito-Hemaglutininas/farmacologia , Linfócitos T Reguladores/imunologiaRESUMO
Natural killer (NK) cell activity and antibody dependent cell-mediated cytotoxicity (ADCC) was measured in 62 untreated cervical carcinoma patients and 25 normal healthy women, using a short-term chromium release assay. A significant reduction in NK and ADCC activity was observed in disseminated disease than in localized disease, when compared with normal donors. The majority of the patients received radiotherapy and both NK and ADCC activity recovered after therapy. Furthermore, interferon-alpha was demonstrated to augment NK activity of peripheral blood mononuclear cells from healthy donors as well as patients. Also large granular lymphocytes separated on Percoll density gradient were the same in number in both the populations studied, although in cervical cancer there seemed to be a defect in killing activity.
Assuntos
Citotoxicidade Celular Dependente de Anticorpos , Células Matadoras Naturais/imunologia , Neoplasias do Colo do Útero/imunologia , Separação Celular , Centrifugação com Gradiente de Concentração , Feminino , Humanos , Imunidade Inata/efeitos dos fármacos , Interferon gama/farmacologia , Metástase Neoplásica , Linfócitos T/efeitos dos fármacos , Linfócitos T/imunologia , Neoplasias do Colo do Útero/radioterapiaRESUMO
Immune complexes (IC) isolated from pleural effusions of lymphomas with favorable and unfavorable prognoses were of IgG type. These IC were further dissociated by ion exchange chromatography using 8 M urea. The antibody was found to be a high molecular weight protein (1.5 X 10(5) daltons) and reacted with antihuman IgG immunologically while a second peak obtained on ion exchange chromatography may be an antigen moiety with a molecular weight of 3.2 X 10(4) daltons as it reacted immunologically with the antibody. Strong cytoplasmic fluorescence was observed with various cell suspensions of lymphomas when reacted with the antibody preparations. The antisera raised against two different antigen fractions prepared from two lymphomas--nHL and LL showed positive fluorescence with both nHL and LL suspensions. The absorption of these rabbit antibodies with individual cell extracts or with antigen preparations also entirely blocked the cytoplasmic staining. The antigen moiety (PK-II) may have a common origin in the disease process. Pleural effusions from patients with unfavorable and favorable prognoses showed identical patterns of separation of IC components.
Assuntos
Complexo Antígeno-Anticorpo/isolamento & purificação , Linfoma não Hodgkin/imunologia , Derrame Pleural/imunologia , Complexo Antígeno-Anticorpo/imunologia , Cromatografia de Afinidade , Cromatografia em Gel , Cromatografia por Troca Iônica , Imunofluorescência , Humanos , Imunoeletroforese , Imunoglobulina G/imunologia , Técnicas de Imunoadsorção , Peso Molecular , PrognósticoRESUMO
Eighty untreated cervical carcinoma patients were tested for their immune status by evaluating levels of circulating T and B lymphocytes and lymphocyte reactivity to PHA. Significant reduction in T lymphocytes and depressed lymphocyte reactivity to PHA was observed following the progression of the disease. However, B lymphocyte levels remained unaffected. The general impairment observed in cell-mediated immunity was found to be more pronounced in the disseminated disease than in the localized group.