RESUMO
The 31P nuclear magnetic resonance (NMR) characteristics, toxicity, and cellular penetration of five linear or cyclic alpha-aminophosphonate highly sensitive pH probes were investigated in Dictyostelium discoideum cells and isolated rat hearts and were compared with three phosphonic acid derivatives. The line width broadening at pH approximately pK(a), which was satisfactorily modelized for all compounds, was significantly limited in biological milieu for the new markers, affording a four- to sixfold better accuracy in pH determination. Cellular uptake or washout of nontoxic concentrations (< 15 mM) of alpha-aminophosphonates occurred by rapid passive permeation, whereas standard probes required a much slower fluid-phase pinocytosis and transport processes that could ultimately lead to trapping. Using mild concentrations (< 4 mM) three alpha-aminophosphonates having 6 < pK(a) < 7 allowed an easy and simultaneous 31P NMR determination of cytosolic, acidic, and extracellular compartments in anoxic-reoxygenated or starving D. discoideum.
Assuntos
Aminas/química , Ressonância Magnética Nuclear Biomolecular/métodos , Organofosfonatos/química , Aminas/farmacocinética , Aminas/toxicidade , Animais , Células Cultivadas , Dictyostelium/química , Dictyostelium/efeitos dos fármacos , Dictyostelium/metabolismo , Coração/efeitos dos fármacos , Concentração de Íons de Hidrogênio , Miocárdio/química , Miocárdio/metabolismo , Neuroglia/química , Neuroglia/efeitos dos fármacos , Neuroglia/metabolismo , Organofosfonatos/farmacocinética , Organofosfonatos/toxicidade , Permeabilidade , Isótopos de Fósforo/análise , Ratos , Frações Subcelulares/química , Frações Subcelulares/metabolismoRESUMO
The social amoeba Dictyostelium discoideum adopts a cohesive stage upon starvation and then produces Discoidin I and II, two proteins able to bind galactose and N-acetyl-galactosamine. The N-terminal domain or discoidin domain (DS) is widely distributed in eukaryotes where it plays a role in extracellular matrix binding while the C-terminal domain displays sequence similarities to invertebrate lectins. We present the first X-ray structures of the wild-type and recombinant Discoidin II in unliganded state and in complex with monosaccharides. The protein forms a homotrimer which presents two binding surfaces situated on the opposite boundaries of the structure. The binding sites of the N-terminal domain contain PEG molecules that could mimics binding of natural ligand. The C-terminal lectin domain interactions with N-acetyl-D-galactosamine and methyl-beta-galactoside are described. The carbohydrate binding sites are located at the interface between monomers. Specificity for galacto configuration can be rationalized since the axial O4 hydroxyl group is involved in several hydrogen bonds with protein side chains. Titration microcalorimetry allowed characterization of affinity and demonstrated the enthalpy-driven character of the interaction. Those results highlight the structural differentiation of the DS domain involved in many cell-adhesion processes from the lectin activity of Dictyostelium discoidins.
Assuntos
Acetilgalactosamina/química , Dictyostelium/metabolismo , Lectinas/química , Proteínas de Protozoários/química , Animais , Sítios de Ligação , Clonagem Molecular , Cristalografia por Raios X , Discoidinas , Lectinas/genética , Estrutura Terciária de Proteína , Proteínas de Protozoários/genética , Proteínas Recombinantes/química , Proteínas Recombinantes/genéticaRESUMO
Proteins of the mitochondrial carrier family (MCF) mediate the transport of a large range of compounds, including metabolites and cofactors. They are localized mainly in the inner mitochondrial membrane, except for a few members found in the membranes of peroxisomes. Similarity searches among Dictyostelium discoideum protein sequences identified a total of 31 MCF members. All these are membrane proteins that possess three characteristic repeats of a domain of approximately 100 residues. Among them, three proteins have supplementary structural domains consisting of Ca(2+)-binding motifs made up of 2 or 4 EF-hand units localized on the N-terminal end, facing the mitochondrial intermembrane space. The nature of transported substrates is proposed on the basis of sequence comparison with orthologs characterized biochemically in other organisms, of phylogenetic analysis, and of the conservation of discriminating amino acid residues belonging to the substrate binding sites. Carriers have been grouped in subclasses based on their specificity for the transport of nucleotides, amino acids or keto acids. Furthermore, we have identified an iron carrier of the mitoferrin type, an inorganic phosphate carrier, and three carriers with similarity to uncoupler proteins. This study provides a focus for mitochondrial carrier analysis in Dictyostelium discoideum.
Assuntos
Dictyostelium/genética , Proteínas de Transporte da Membrana Mitocondrial/genética , Sequência de Aminoácidos , Animais , Sítios de Ligação/genética , Translocases Mitocondriais de ADP e ATP/química , Translocases Mitocondriais de ADP e ATP/genética , Proteínas de Transporte da Membrana Mitocondrial/química , Modelos Moleculares , Dados de Sequência Molecular , Filogenia , Estrutura Secundária de Proteína , Estrutura Terciária de Proteína , Homologia de Sequência de AminoácidosRESUMO
Alix is a phylogenetically conserved protein that participates in mammals in programmed cell death in association with ALG-2, a penta-EF-hand calciprotein. It contains an N-terminal Bro1 domain, a coiled-coil region and a C-terminal proline-rich domain containing several SH3- and WW-binding sites that contribute to its scaffolding properties. Recent data showed that by virtue of its Bro1 domain, Alix is functionally associated to the ESCRT complexes involved in the biogenesis of the multivesicular body and sorting of transmembrane proteins within this specific endosomal compartment. In Dictyostelium, an alx null strain shows a markedly perturbed starvation-induced morphogenetic program while ALG-2 disruptants remain unaffected. This review summarizes Dictyostelium data on Alix and ALG-2 homologues and evaluates whether known functions of Alix in other organisms can account for the developmental arrest of the alx null mutant and how Dictyostelium studies can substantiate the current understanding of the function(s) of this versatile and conserved signaling molecule.
Assuntos
Dictyostelium/crescimento & desenvolvimento , Dictyostelium/metabolismo , Proteínas de Drosophila/metabolismo , Proteínas dos Microfilamentos/metabolismo , Proteínas de Protozoários/metabolismo , Transdução de Sinais/fisiologia , Animais , Proteínas Reguladoras de Apoptose/química , Proteínas Reguladoras de Apoptose/genética , Proteínas Reguladoras de Apoptose/metabolismo , Proteínas de Ligação ao Cálcio/química , Proteínas de Ligação ao Cálcio/genética , Proteínas de Ligação ao Cálcio/metabolismo , Morte Celular/fisiologia , Dictyostelium/genética , Proteínas de Drosophila/química , Proteínas de Drosophila/genética , Proteínas dos Microfilamentos/química , Proteínas dos Microfilamentos/genética , Modelos Moleculares , Estrutura Terciária de Proteína , Proteínas de Protozoários/química , Proteínas de Protozoários/genéticaRESUMO
Application of hydrodynamic mild shear stress to adherent Dictyostelium discoideum vegetative cells triggers active actin cytoskeleton remodeling resulting in net cell movement along the flow. The average cell speed is strongly stimulated by external calcium (Ca2+, K50%=22 microM), but the directionality of the movement is almost unaffected. This calcium concentration is ten times higher than the one promoting cell adhesion to glass surfaces (K50%=2 microM). Addition of the calcium chelator EGTA or the Ca2+-channel blocker gadolinium (Gd3+) transiently stops cell movement. Monitoring the evolution of cell-surface contact area with time reveals that calcium stimulates cell speed by increasing the amplitude of both protrusion and retraction events at the cell edge, but not the frequency. As a consequence, with saturating external calcium concentrations, cells are sensitive to very low shear forces (20 pN; sigma=0.1 Pa). Moreover, a null-mutant lacking the unique Gbeta subunit does not respond to external Ca2+ changes (K50%>1000 microM), although the directionality of the movement is comparable with that of wild-type cells. Furthermore, cells lacking the inositol 1,4,5-trisphosphate receptor (IP3-receptor) exhibit a markedly reduced Ca2+ sensitivity. Thus, calcium release from internal stores and calcium entry through the plasma membrane modulate cell speed in response to shear stress.
Assuntos
Cálcio/metabolismo , Dictyostelium/metabolismo , Fluidez de Membrana/fisiologia , Actinas/química , Actinas/fisiologia , Animais , Cálcio/antagonistas & inibidores , Cálcio/farmacologia , Adesão Celular/fisiologia , Linhagem Celular , Citoesqueleto/fisiologia , Dictyostelium/química , Dictyostelium/citologia , Relação Dose-Resposta a Droga , Ácido Egtázico/farmacologia , Gadolínio/química , Gadolínio/farmacologia , Vidro , Fluidez de Membrana/efeitos dos fármacos , Modelos Químicos , Movimento/efeitos dos fármacos , Movimento/fisiologia , Sensibilidade e Especificidade , Resistência ao Cisalhamento , Propriedades de Superfície , Fatores de TempoRESUMO
Membrane-associated NADPH oxidase complexes catalyse the production of the superoxide anion radical from oxygen and NADPH. In mammalian systems, NADPH oxidases form a family of at least seven isoforms that participate in host defence and signalling pathways. We report here the cloning and the characterisation of slime mould Dictyostelium discoideum homologs of the mammalian heme-containing subunit of flavocytochrome b (gp91(phox)) (NoxA, NoxB and NoxC), of the small subunit of flavocytochrome b (p22(phox)) and of the cytosolic factor p67(phox). Null-mutants of either noxA, noxB, noxC or p22(phox) show aberrant starvation-induced development and are unable to produce spores. The overexpression of NoxA(myc2) in noxA null strain restores spore formation. Remarkably, the gene alg-2B, coding for one of the two penta EF-hand proteins in Dictyostelium, acts as a suppressor in noxA, noxB, and p22(phox) null-mutant strains. Knockout of alg-2B allows noxA, noxB or p22(phox) null-mutants to return to normal development. However, the knockout of gene encoding NoxC, which contains two penta EF-hands, is not rescued by the invalidation of alg-2B. These data are consistent with a hypothesis connecting superoxide and calcium signalling during Dictyostelium development.
Assuntos
Diferenciação Celular , Dictyostelium/enzimologia , Morfogênese , NADPH Oxidases/metabolismo , Sequência de Aminoácidos , Animais , Catálise , Membrana Celular/enzimologia , Células Cultivadas , Clonagem Molecular , Dictyostelium/citologia , Dictyostelium/crescimento & desenvolvimento , Motivos EF Hand , Regulação da Expressão Gênica no Desenvolvimento , Genes de Protozoários , Isoenzimas/genética , Isoenzimas/metabolismo , Modelos Moleculares , Dados de Sequência Molecular , Mutação , NADPH Oxidases/química , NADPH Oxidases/genética , Filogenia , Estrutura Secundária de Proteína , Estrutura Terciária de Proteína , Análise de Sequência de DNA , Homologia de Sequência de Aminoácidos , Superóxidos/metabolismoRESUMO
We have characterized the Dictyostelium homolog of the mammalian protein Alix. Dd-Alix is encoded by a single gene and is expressed during vegetative growth and multicellular development. We showed that the alx null strain fails to complete its developmental program. Past the tight aggregate stage, morphogenesis is impaired, leading to markedly aberrant structures containing vacuolated and undifferentiated cells but no mature spores. The developmental defect is cell-autonomous as most cells remain of the PstB type even when mixed with wild-type cells. Complementation analysis with different Alix constructs allowed the identification of a 101-residue stretch containing a coiled-coil domain essential for Alix function. In addition, we showed that the protein associates in part with vesicular structures and that its distribution on a Percoll gradient overlaps that of the endocytic marker Vamp7. Dd-Alix also co-localizes with Dd-Vps32. In view of our data, and given the role of Vps32 proteins in membrane protein sorting and multivesicular body formation in yeast and mammals, we hypothesize that the developmental defects of the alx null strain result from abnormal trafficking of cell-surface receptors.
Assuntos
Dictyostelium/crescimento & desenvolvimento , Proteínas de Protozoários/genética , Sequência de Aminoácidos , Animais , Biotinilação , Sequência Conservada , Dictyostelium/citologia , Endossomos/metabolismo , Regulação da Expressão Gênica no Desenvolvimento , Humanos , Mamíferos , Dados de Sequência Molecular , Plasmídeos , Proteínas de Protozoários/metabolismo , Alinhamento de Sequência , Homologia de Sequência de AminoácidosRESUMO
Application of a mild hydrodynamic shear stress to Dicytostelium discoideum cells, unable to detach cells passively from the substrate, triggers a cellular response consisting of steady membrane peeling at the rear edge of the cell and periodic cell contact extensions at its front edge. Both processes require an active actin cytoskeleton. The cell movement induced by the hydrodynamic forces is very similar to amoeboid cell motion during chemotaxis, as for its kinematic parameters and for the involvement of phosphatidylinositol(3,4,5)-trisphosphate internal gradient to maintain cell polarity. Inhibition of phosphoinositide 3-kinases by LY294002 randomizes the orientation of cell movement with respect to the flow without modifying cell speed. Two independent signaling pathways are, therefore, induced in D. discoideum in response to external forces. The first increases the frequency of pseudopodium extension, whereas the second redirects the actin cytoskeleton polymerization machinery to the edge opposite to the stressed side of the cell.
Assuntos
Quimiotaxia/fisiologia , Dictyostelium/fisiologia , Transdução de Sinais/fisiologia , Actinas/fisiologia , Animais , Adesão Celular , Cromonas/farmacologia , Citoesqueleto/fisiologia , Inibidores Enzimáticos/farmacologia , Morfolinas/farmacologia , Fosfatidilinositol 3-Quinases/metabolismo , Fosfatidilinositóis/metabolismo , Inibidores de Fosfoinositídeo-3 Quinase , Resistência ao CisalhamentoRESUMO
The macropinocytic pathway in Dictyostelium discoideum is organized linearly. After actin-driven internalization, fluid material passes sequentially from endosomes to lysosomes, where molecules are degraded and absorbed. Residual material is exocytosed via post-lysosomal compartments. Syntaxin 7 is a SNARE (soluble N -ethylmaleimide-sensitive fusion protein attachment protein receptor) protein that is present and active in D. discoideum endosomes [Bogdanovic, Bruckert, Morio and Satre (2000) J. Biol. Chem. 275, 36691-36697]. Here we report the identification of its main SNARE partners by co-immunoprecipitation and MS peptide sequencing. The syntaxin 7 complex contains two co-t-SNAREs [Vti1 (Vps10p tail interactor 1) and syntaxin 8] and a v-SNARE [VAMP7 (vesicle-associated membrane protein 7)] (where t-SNAREs are SNAREs of the target compartment and v-SNAREs are SNAREs present in donor vesicles). In endosomes and in vitro, syntaxin 7, Vti1 and syntaxin 8 form a complex that is able to bind VAMP7. Antibodies to syntaxin 8 and a soluble recombinant VAMP7 fragment both inhibit in vitro reconstituted D. discoideum endosome fusion. The lysosomal content of syntaxin 7, Vti1, syntaxin 8 and VAMP7 is low compared with that in endosomes, implying a highly active recycling or retention mechanism. A likely model is that VAMP7 is a v-SNARE present on vesicles carrying lysosomal enzymes, and that the syntaxin 7-Vti1-syntaxin 8 t-SNARE complex is associated with incoming endocytic material.
Assuntos
Proteínas de Transporte/metabolismo , Dictyostelium/metabolismo , Proteínas de Membrana/metabolismo , Proteínas de Protozoários/metabolismo , Proteínas de Transporte Vesicular , Sequência de Aminoácidos , Animais , Endossomos/metabolismo , Humanos , Fusão de Membrana , Dados de Sequência Molecular , Pinocitose , Proteínas Qa-SNARE , Proteínas Qb-SNARE , Proteínas R-SNARE , Proteínas SNARERESUMO
Two homologues, Dd-ALG-2a and Dd-ALG-2b, of the mammalian calcium-binding protein ALG-2 (apoptosis-linked gene 2) have been characterized in the cellular slime mold Dictyostelium discoideum. Fluorescence titrations showed that both proteins bind calcium ions with affinities (Ca2+)(0.5) of 30 and 450 microm, respectively, at sites specific to calcium. Calcium ion binding resulted in changes of conformation associated with the unmasking of hydrophobic regions of the proteins. Surface plasmon resonance analysis showed that Dd-ALG-2a homodimers formed (K(D) of 1 microm) at calcium ion concentrations similar to those necessary for Ca2+-induced conformational changes. Deletion of the hydrophobic N-terminal sequence or EF-hand 5 of Dd-ALG-2a prevented dimerization. The Dd-ALG-2b homodimer was not detected, and the Dd-ALG-2a/2b heterodimer formed only when Dd-ALG-2b was the immobilized partner. Murine Alix formed a heterodimer (K(D) = 0.6 microm) with Dd-ALG-2a but not with Dd-ALG-2b, and the interaction strictly depended upon calcium ions. The DeltaNter construct of Dd-ALG-2a lost its interaction capacity with mouse Alix. The genes encoding both proteins, Dd-alg-2a and -2b, were expressed in growing cells. The levels of mRNA were at a maximum during aggregation (4-8 h) and decreased rapidly thereafter. In contrast, the levels of proteins remained fairly stable. Dd-ALG-2a and Dd-ALG-2b were found to be dispensable for growth and development, based on the finding that single Dd-alg2a- or Dd-alg-2b- and double Dd-alg2a-/Dd-alg-2b- mutant cell lines showed normal growth in axenic medium or on bacterial lawns and exhibited unaltered development.
Assuntos
Proteínas de Ligação ao Cálcio/química , Proteínas de Transporte/química , Dictyostelium/química , Sequência de Aminoácidos , Animais , Proteínas Reguladoras de Apoptose , Western Blotting , Cálcio/metabolismo , Proteínas de Ligação ao Cálcio/genética , Proteínas de Ligação ao Cálcio/metabolismo , Proteínas de Transporte/genética , Células Cultivadas , Clonagem Molecular , DNA Complementar/metabolismo , Dictyostelium/genética , Relação Dose-Resposta a Droga , Cinética , Camundongos , Microscopia de Fluorescência , Dados de Sequência Molecular , Mutação , Plasmídeos/metabolismo , Ligação Proteica , Conformação Proteica , RNA Mensageiro/metabolismo , Proteínas Recombinantes/metabolismo , Homologia de Sequência de Aminoácidos , Espectrometria de Fluorescência , Ressonância de Plasmônio de Superfície , Fatores de TempoRESUMO
Among the different assays to measure cell adhesion, shear-flow detachment chambers offer the advantage to study both passive and active aspects of the phenomena on large cell numbers. Mathematical modeling allows full exploitation of the data by relating molecular parameters to cell mechanics. Using D. discoideum as a model system, we explain how cell detachment kinetics gives access to the rate constants describing the passive association or dissociation of the cell membrane to a given substrate.