Gastrointestinal parasites in shelter cats of central Italy.

In Italy, the limited resources and the large number of cats living in feline shelters may facilitate the spread of parasite infections that may affect the health of cats and expose shelter workers and adoptive owners to zoonosis. Prevalence and risk factors of potential zoonotic and host-specific gastrointestinal parasite infections were assessed in cats living in public and private shelters of central Italy. Individual fecal samples were examined macroscopically and then screened microscopically by fresh and Lugol stained fecal smears and by flotation and the Baermann tests. A commercial rapid immune-chromatographic assay was used to detect Giardia duodenalis and Cryptosporidium spp. All animals were clinically examined to evaluate presence of clinical signs compatible to parasitic infections. Data were statistically analysed by multivariate analysis with logistic regression and chi square testing for bivariate analysis. The overall prevalence of gastrointestinal parasites in the shelter cats surveyed was 22% (29/132), with 26/132 (19.7%) of the animals infected with potentially zoonotic organisms. Upon statistical analysis, parasite infections were identified in significantly more cats younger than 18 months of age (p <0.05), and most often associated with the presence of compatible clinical signs (p <0.05). A higher prevalence of protozoan infections (18.1%, 24/132) than of helminth infections (12.9%, 17/132) was observed. Identified parasites were Giardia duodenalis (10.6%, 14/132), Toxocara cati (9%, 12/132), Cystoisospora felis (3%, 4/132), Cystoisospora rivolta (2.3%, 3/132), hookworms (2.3%, 3/132), Cryptosporidium spp. (1.6%, 2/132), Aonchotheca putorii (0.75%, 1/132), Tritrichomonas foetus (0.75%, 1/132) and Strongyloides sp. (0.75%, 1/132). Co-infections were identified in the 7.6% of animals surveyed. Results obtained from this study indicate high gastrointestinal parasite risk to shelter cats and public health in the examined areas and suggest the need for more effective control measures in shelters surveyed.

Leishmania infantum, Dirofilaria spp. and other endoparasite infections in kennel dogs in central Italy.

Prevalence and risk factors of Leishmania infantum, Dirofilaria spp. and other potentially zoonotic or canine-specific endoparasite infections were assessed in 639 kennel dogs from central Italy. To this end, individual blood and fecal samples were examined using parasitological, immunological and molecular techniques. The presence of compatible clinical pictures, as well as age and gender were considered as putative risks factors. To evaluate risk factors, multivariable analysis with logistic regression and univariable analysis with a Chi square test and a Fischer's exact test were performed. Overall, 52.6% of dogs (95% CI 48.6-56.5) were found positive, while 39.6% of dogs (95% CI 35.8-43.5) were infected by potentially zoonotic species. Leishmania infantum and Dirofilaria repens showed prevalences of 2.5% (95% CI 1.5-4.1) and 2.8% (95% CI 1.7-4.5), respectively. The prevalence of cardiorespiratory parasites was 7.8% (95% CI 5.9-10.3) and included the species Angiostrongylus vasorum, Eucoleus aerophilus, Eucoleus boehmi and D. immitis; the latter showed a prevalence of 0.2% (95% CI 0.001-1). Intestinal parasites were significantly prevalent (38.8%, 95% CI 35-42.7) and they consisted mainly of species of major zoonotic concern, including ancylostomatids, Toxocara canis, Giardia duodenalis, Dipylidium caninum, Taeniidae, Strongyloides stercoralis and Cryptosporidium parvum. Endoparasites were significantly prevalent in clinically suspected dogs. Leishmania infantum and cardiorespiratory nematodes were prevalent in older dogs, while intestinal parasites were prevalent in younger dogs. Results show high dog and public health risks in kennels in central Italy, and suggest the need for more effective control measures.