Effect of sex difference on platelet aggregation using an optical method in healthy subjects.

There are many studies reporting conflicting results of sex differences on various platelet functions. The purpose of this study was to investigate whether sex differences could affect platelet aggregation results using an optical method in healthy subjects. A total of 42 subjects, 21 males and 21 females, were included in the study. Platelet aggregation was induced by adenosine diphosphate (ADP; 5 microm), collagen (2 microg/ml), and epinephrine (10 microm). Optical aggregation was performed using a turbidometric method. In all platelet aggregation tests ADP, collagen and epinephrine were studied; there was no significant difference between females and males in platelet aggregation amplitudes and slopes. As a result, sex difference does not affect platelet aggregation performed with this optical method in healthy subjects. This result supports that there is no need for sex differentiation while composing control groups in platelet aggregation studies using the optical method.

Investigation of separation and identification possibilities of some metal-DEDTC complexes by sequential TLC-IR system.

The separation and identification possibilities of some metal-diethyldithiocarbamate (DEDTC) complexes are investigated by thin-layer chromatography-IR sequential system. DEDTC complexes of metal cations are prepared in a predetermined optimum pH or pH range. Optimum separation conditions of these complexes and the reproducibilities of their retention factor (Rf) values are investigated on a series of precoated or laboratory-made TLC plates. Rf values are determined using the one-dimensional ascending technique. The precision of the procedures is determined for five replicates in terms of standard deviation. Detection limits are also determined for each of the metal complexes. The IR spectra are scanned and interpreted for their precise identifications after preconcentration with a wick-stick procedure for the complexes, giving the overlapped Rf values. The complexes could be identified by the aid of IR absorption bands. It has been determined that these IR absorption bands can also be used as reference spectral bands in possible quantitative studies. It is concluded that an efficient and successful qualitative analysis is possible for incompletely-separated complex compounds using an IR spectrophotometer as the TLC detector.

Extraction of some transition metals with N,N-dibutyl-N'-benzoylthiourea into molten paraffin at 65 degrees C.

The solid-liquid extraction behaviour of some transition metals (Cu(II), Co(II), Ni(II), Cd(II) and Pb(II)) by the use of N,N-dibutyl-N'-benzoylthiourea as an extractant in paraffin (58-60 degrees C) has been investigated at 65.0+/-0.5 degrees C. The effect of equilibration time, pH of the aqueous phase, concentration of extractant and solid solvent used on the extraction efficiency of these metals have been discussed. The interferences of various ions are examined. The method has been applied to the determination of these metals in synthetic samples, mushrooms and sediment.