RESUMO
The aging-associated secretory phenotype (SASP) is a heterogeneous phenotype of cells secreting pro-inflammatory cytokines, growth factors, apoptosis' regulatory molecules, and proteases. SASP is one of the three main hallmarks of senescent cells. Dysregulation of the synthesis of SASP-forming molecules leads to the development of age-associated diseases, including cardiovascular pathology. The aim of this study is to characterize the SASP of human endotheliocytes during replicative and induced aging. Isolated human umbilical vein endothelial cells HUVEC were used to model replicative and inflammation-induced aging. It has been established that the molecules that form SASP during replicative and inflammation-induced aging of HUVEC are molecules that control apoptosis (p16, p21, p53), adhesion (E-selectin, VCAM-1) and some cytokines (IL-1ß, IL-6). With replicative aging of endotheliocytes, the synthesis of apoptosis' regulatory molecules increases to a greater extent. Inflammation-induced aging of HUVEC is characterized by a multiple increase in the synthesis of adhesion molecules and pro-inflammatory cytokines.
Assuntos
Senescência Celular , Células Endoteliais , Humanos , Células Endoteliais/metabolismo , Senescência Celular/fisiologia , Envelhecimento/fisiologia , Fenótipo , Inflamação/metabolismo , CitocinasRESUMO
Senescent cells take part into development the age-related diseases by formation the SASP: senescence-associated secretory phenotype. SASP is characterized by synthesis of signal molecules include proinflammatory cytokines. SASP promotes the inflammaging - chronic, low-grade, subclinical inflammatory process, that is the one of cardio-vascular diseases risk factor in older age-groups. In the review we describe the key SASP molecules of cardiomyocytes, endotheliocytes and vascular smooth muscle cells and its role in the pathogenesis of age-associated cardiovascular diseases.