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1.
Rheumatol Int ; 32(1): 259-62, 2012 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-21240498

RESUMO

This study was conducted in Turkish osteoarthritis patients to determine the frequency of 4G/5G polymorphism genotypes of plasminogen activator inhibitor type-1 gene and to examine the role of this polymorphism in osteoarthritis development. Genomic DNA obtained from 200 persons (140 patients with osteoarthritis and 60 healthy controls) was used in the study. DNA was amplified by polymerase chain reaction using 4G allele- and 5G allele-specific primers. Polymerase chain reaction products were assessed with CCD camera by being exposed to 2% agarose gel electrophoresis. No statistically significant difference between the groups with respect to genotype distribution was found (P > 0.05) in the study. The 4G allele frequency was indicated as 44% and 5G allele was as 56% in patients, whereas this was 45-55% in the control group. This study has established that 4G/5G polymorphism genotypes of plasminogen activator inhibitor type-1 gene do not play a role in the development of osteoarthritis in the Turkish population.


Assuntos
Osteoartrite/genética , Inibidor 1 de Ativador de Plasminogênio/genética , Polimorfismo Genético/genética , Adulto , Estudos de Casos e Controles , Feminino , Frequência do Gene/genética , Genótipo , Humanos , Masculino , Pessoa de Meia-Idade , Osteoartrite/etnologia , Turquia
2.
Mol Biol Rep ; 38(3): 1713-6, 2011 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-20845076

RESUMO

This study was conducted in Turkish osteoarthritis patients to determine the frequency of I/D polymorphism genotypes of angiotensin converting enzyme gene, and to examine the role of this polymorphism in osteoarthritis development. Genomic DNA obtained from 200 persons (135 patients with osteoarthritis and 65 healthy controls) was used in the study. DNA was multiplied by polymerase chain reaction using I and D allele-specific primers. Polymerase chain reaction products were assessed with CCD camera by being exposed to 2% agarose gel electrophoresis. There was statistically significant difference between the groups with respect to genotype distribution (P < 0.001). The D allele frequency was indicated as 69% and I allele was as 31% in the patients, whereas it was 55-45% in the control group. Consequently, in this study, we may assert that ACE gene I/D polymorphism DD genotype determination is significant criteria for identifying patients who are likely to develop osteoarthritis in east population of Turkey.


Assuntos
Estudos de Associação Genética , Predisposição Genética para Doença , Mutação INDEL/genética , Osteoartrite/enzimologia , Osteoartrite/genética , Peptidil Dipeptidase A/genética , Polimorfismo Genético , Adulto , Estudos de Casos e Controles , Feminino , Frequência do Gene/genética , Humanos , Masculino , Pessoa de Meia-Idade , Reação em Cadeia da Polimerase , Turquia
3.
Arch Orthop Trauma Surg ; 129(9): 1225-31, 2009 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-19057919

RESUMO

INTRODUCTION: Our goal in this study was to propose an alternative closed reduction method to avoid open reduction in the management of pediatric supracondylar humerus fractures. A temporary Kirschner wire is inserted to the proximal part of the humerus to be used as a joystick to have a better control of the proximal fragment. PATIENTS: Twenty-three patients with closed Gartland type III extension fractures were operated between 2003 and 2007. Mean age of the patients was 6.7 (4-10) years. METHOD: Uninjured and fractured elbow's Bauman angles, lateral condylar angles, the carrying angles and ROM values were compared statistically according to independent samples t test. RESULTS: At a mean follow-up 96.73 weeks (53-150), clinical results using the Flynn criteria were excellent in 21 (91.3%), good in 1(4.35%), fair in 1 (4.35%) patient. CONCLUSION: These results suggest that the "joystick" reduction method is a reliable alternative when closed reduction itself is not successful.


Assuntos
Fios Ortopédicos , Fixação de Fratura/métodos , Fraturas do Ombro/cirurgia , Criança , Pré-Escolar , Feminino , Fluoroscopia , Humanos , Masculino , Complicações Pós-Operatórias/prevenção & controle , Amplitude de Movimento Articular , Resultado do Tratamento
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