RESUMO
Surface water concentrations of 54 pharmaceuticals were predicted for seven major Swedish rivers and the Stockholm City area basins using the STREAM-EU model. These surface water concentrations were used to predict the ecotoxicological impact resulting from the exposure of aquatic organisms to this mixture of 54 pharmaceuticals. STREAM-EU model results indicated that <10 substances were present at median annual water concentrations greater than 10â¯ng/L with highest concentrations occurring mostly in the more densely populated area of the capital city, Stockholm. There was considerable spatial and temporal variability in the model predictions (1-3 orders of magnitude) due to natural variability (e.g. hydrology, temperature), variations in emissions and uncertainty sources. Local mixture ecotoxicological pressures based on acute EC50 data as well as on chronic NOEC data, expressed as multi-substance potentially affected fraction of species (msPAF), were quantified in 114 separate locations in the waterbodies. It was estimated that 5% of the exposed aquatic species would experience exposure at or above their acute EC50 concentrations (so-called acute hazardous concentration for 5% of species, or aHC5) at only 7% of the locations analyzed (8 out of 114 locations). For the evaluation based on chronic NOEC concentrations, the chronic HC5 (cHC5) is exceeded at 27% of the locations. The acute mixture toxic pressure was estimated to be predominantly caused by only three substances in all waterbodies: Furosemide, Tramadol and Ibuprofen. A similar evaluation of chronic toxic pressure evaluation logically demonstrates that more substances play a significant role in causing a higher chronic toxic pressure at more sites as compared to the acute toxic pressure evaluation. In addition to the three substances contributing most to acute effects, the chronic effects are predominantly caused by another five substances: paracetamol, diclofenac, ethinylestradiol, erythromycin and ciprofloxacin. This study provides regulatory authorities and companies responsible for water quality valuable information for targeting remediation measures and monitoring on a substance and location basis.
Assuntos
Organismos Aquáticos/efeitos dos fármacos , Ecotoxicologia , Monitoramento Ambiental/métodos , Preparações Farmacêuticas/análise , Medição de Risco/métodos , Poluentes Químicos da Água/análise , Poluentes Químicos da Água/toxicidade , Animais , Organismos Aquáticos/crescimento & desenvolvimento , Água Doce , Preparações Farmacêuticas/metabolismo , Suécia , Testes de ToxicidadeRESUMO
The present publication describes an integrative grouping concept to derive threshold values for inhalation exposure. The classification scheme starts with differences in toxicological potency and develops criteria to group compounds into two potency classes, namely toxic (T-group) or low toxic (L-group). The TTC concept for inhalation exposure is based on the TTC RepDose data set, consisting of 296 organic compounds with 608 repeated-dose inhalation studies. Initially, 21 structural features (SFs) were identified as being characteristic for compounds of either high or low NOEC values (Schüürmann et al., 2016). In subsequent analyses these SF groups were further refined by taking into account structural homogeneity, type of toxicological effect observed, differences in absorption, metabolism and mechanism of action (MoA), to better define their structural and toxicological boundaries. Differentiation of a local or systemic mode of action did not improve the classification scheme. Finally, 28 groups were discriminated: 19 T-groups and 9 L-groups. Clearly distinct thresholds were derived for the T- and L-toxicity groups, being 2 × 10(-5) ppm (2 µg/person/day) and 0.05 ppm (4260 µg/person/day), respectively. The derived thresholds and the classification are compared to the initial mainly structure driven grouping (Schüürmann et al., 2016) and to the Cramer classification.
Assuntos
Mineração de Dados/métodos , Substâncias Perigosas/toxicidade , Exposição por Inalação/efeitos adversos , Modelos Moleculares , Compostos Orgânicos/toxicidade , Testes de Toxicidade/métodos , Absorção Fisiológica , Animais , Bases de Dados Factuais , Relação Dose-Resposta a Droga , Substâncias Perigosas/química , Substâncias Perigosas/classificação , Substâncias Perigosas/farmacocinética , Humanos , Estrutura Molecular , Nível de Efeito Adverso não Observado , Compostos Orgânicos/química , Compostos Orgânicos/classificação , Compostos Orgânicos/farmacocinética , Reconhecimento Automatizado de Padrão , Medição de Risco , Relação Estrutura-AtividadeRESUMO
The present study is motivated by the increasing demand to consider internal partitioning into tissues instead of exposure concentrations for the environmental toxicity assessment. To this end, physiologically based pharmacokinetic (PBPK) models can be applied. We evaluated the variation in accuracy of PBPK model outcomes depending on tissue constituents modeled as sorptive phases and chemical distribution tendencies addressed by molecular descriptors. The model performance was examined using data from 150 experiments for 28 chemicals collected from US EPA databases. The simplest PBPK model is based on the "Kow-lipid content" approach as being traditional for environmental toxicology. The most elaborated one considers five biological sorptive phases (polar and non-polar lipids, water, albumin and the remaining proteins) and makes use of LSER (linear solvation energy relationship) parameters to describe the compound partitioning behavior. The "Kow-lipid content"-based PBPK model shows more than one order of magnitude difference in predicted and measured values for 37% of the studied exposure experiments while for the most elaborated model this happens only for 7%. It is shown that further improvements could be achieved by introducing corrections for metabolic biotransformation and compound transmission hindrance through a cellular membrane. The analysis of the interface distribution tendencies shows that polar tissue constituents, namely water, polar lipids and proteins, play an important role in the accumulation behavior of polar compounds with H-bond donating functional groups. For compounds without H-bond donating fragments preferable accumulation phases are storage lipids and water depending on compound polarity.
Assuntos
Modelos Biológicos , Oncorhynchus mykiss/fisiologia , Poluentes Químicos da Água/metabolismo , Animais , Biotransformação , Monitoramento Ambiental , CinéticaRESUMO
The vision of a sustainable and safe use of chemicals to protect human health, preserve the environment and maintain the ecosystem requires innovative and more holistic approaches to risk assessment (RA) in order to better inform decision making. Integrated risk assessment (IRA) has been proposed as a solution to current scientific, societal and policy needs. It is defined as the mutual exploitation of environmental risk assessment (ERA) for human health risk assessment (HHRA) and vice versa in order to coherently and more efficiently characterize an overall risk to humans and the environment for better informing the risk analysis process. Extrapolating between species which are relevant for HHRA and ERA requires a detailed understanding of pathways of toxicity/modes of action (MoA) for the various toxicological endpoints. Significant scientific advances, changes in chemical legislation, and increasing environmental consciousness have created a favourable scientific and regulatory environment to develop and promote the concept and vision of IRA. An initial proof of concept is needed to foster the incorporation of IRA approaches into different chemical sectorial regulations and demonstrate their reliability for regulatory purposes. More familiarity and confidence with IRA will ultimately contribute to an overall reduction in in vivo toxicity testing requirements. However, significant progress will only be made if long-term support for MoA-related research is secured. In the short term, further exchange and harmonization of RA terminology, models and methodologies across chemical categories and regulatory agencies will support these efforts. Since societal values, public perceptions and cultural factors are of increasing importance for the acceptance of risk analysis and successful implementation of risk mitigation measures, the integration of socio-economic analysis and socio-behavioural considerations into the risk analysis process may help to produce a more effective risk evaluation and consideration of the risks and benefits associated with the use of chemicals.
Assuntos
Monitoramento Ambiental/métodos , Política Ambiental , Poluentes Ambientais/toxicidade , Monitoramento Ambiental/legislação & jurisprudência , União Europeia , Substâncias Perigosas/toxicidade , Medição de Risco/métodosRESUMO
The mycotoxins patulin, gliotoxin and sterigmatocystin can be produced by common indoor moulds and enter the human body via inhalation of mycotoxin containing spores and particulates. There are various studies about the individual effects of these mycotoxins, but a lack of knowledge about their effects in mixtures. The aim of this study was to evaluate combined effects on the singe celled organism Tetrahymena pyriformis. Using the MIXTOX model (EU project NOMIRACLE) synergistic or antagonistic effects with dose level deviation or dose ratio dependent deviation were analyzed. The most toxic compound gliotoxin (EC50 0.37 µM) and patulin (EC50 9.3 µM) as shown by the MIXTOX model acted synergistic, caused by similar mode of actions. Within the combination with sterigmatocystin (maximum inhibition of 45% at 12.5 µM) antagonistic effects were observed with switch to synergism if the toxicity of the mixture is mainly caused by sterigmatocystin. In the end the MIXTOX model was applicable for the prediction of combined effects of toxic compounds.
Assuntos
Gliotoxina/toxicidade , Modelos Biológicos , Patulina/toxicidade , Esterigmatocistina/toxicidade , Tetrahymena pyriformis/efeitos dos fármacos , Interações Medicamentosas , Gliocladium , Gliotoxina/administração & dosagem , Patulina/administração & dosagem , Esterigmatocistina/administração & dosagem , Tetrahymena pyriformis/crescimento & desenvolvimentoRESUMO
For more than a decade, the integration of human and environmental risk assessment (RA) has become an attractive vision. At the same time, existing European regulations of chemical substances such as REACH (EC Regulation No. 1907/2006), the Plant Protection Products Regulation (EC regulation 1107/2009) and Biocide Regulation (EC Regulation 528/2012) continue to ask for sector-specific RAs, each of which have their individual information requirements regarding exposure and hazard data, and also use different methodologies for the ultimate risk quantification. In response to this difference between the vision for integration and the current scientific and regulatory practice, the present paper outlines five medium-term opportunities for integrating human and environmental RA, followed by detailed discussions of the associated major components and their state of the art. Current hazard assessment approaches are analyzed in terms of data availability and quality, and covering non-test tools, the integrated testing strategy (ITS) approach, the adverse outcome pathway (AOP) concept, methods for assessing uncertainty, and the issue of explicitly treating mixture toxicity. With respect to exposure, opportunities for integrating exposure assessment are discussed, taking into account the uncertainty, standardization and validation of exposure modeling as well as the availability of exposure data. A further focus is on ways to complement RA by a socio-economic assessment (SEA) in order to better inform about risk management options. In this way, the present analysis, developed as part of the EU FP7 project HEROIC, may contribute to paving the way for integrating, where useful and possible, human and environmental RA in a manner suitable for its coupling with SEA.
Assuntos
Exposição Ambiental , Substâncias Perigosas/toxicidade , Medição de Risco/métodos , Testes de Toxicidade , Alternativas aos Testes com Animais , Animais , Exposição Ambiental/efeitos adversos , Exposição Ambiental/análise , União Europeia , Regulamentação Governamental , Humanos , Medição de Risco/legislação & jurisprudência , Medição de Risco/tendências , Fatores Socioeconômicos , Testes de Toxicidade/economia , Testes de Toxicidade/métodos , Testes de Toxicidade/normasRESUMO
Accelerated solvent extraction (ASE) is one of the most recent solid-phase extraction methods and has caught on all over the world in numerous laboratories. Until now it was not known that this device is also very suitable for performing dialysis. In this study, development of a rapid dialysis procedure (RDP) was described that is based on the dialysis of persistent organic xenobiotics from triolein-containing semipermeable membrane devices (SPMDs) using ASE. All the operating parameters were optimized within the framework of usage. The RDP procedure was compared with the conventional dialytic recovery of target analytes under atmospheric pressure using spiked analytes and real field samples of SPMDs exposed to urban air. The main advantages of the RDP in comparison to the conventional dialysis are the speed, with up to 70 times faster taking only 40 min, and the considerable reduction in solvent consumption (by two-thirds) when SPMDs with standard configuration are used. Moreover, the RDP is also suitable as an analytical cleanup procedure for the same analytes from various types of lipid samples and other difficult matrixes using semipermeable membranes.
RESUMO
A new one-step cleanup procedure, based on size-exclusion chromatography (SEC), usable for the extracts from accelerated solvent extraction (ASE), Soxhlet extraction, or ultrasonic extraction (USE), is described. The method is suitable for the determination of polycyclic aromatic hydrocarbons (PAHs), especially from very complicated plant matrices (e.g. pine needles, deciduous leaves, mosses). The main improvement compared with previous conventional procedures is that analyte peaks barely overlap with matrix peaks in the chromatograms and that it is a very rapid and simple one-step procedure with clearly improved analytical performance. Essential advantages of this SEC procedure are the sharper GC-MS chromatograms for the PAH fraction at retention times between 9.2 and 12.0 min, distinctly separated substance peaks resulting in better analysis, shorter running times, and lower solvent consumption.
Assuntos
Acer/química , Cromatografia em Gel/métodos , Pinus/química , Hidrocarbonetos Policíclicos Aromáticos/análise , Cromatografia Gasosa-Espectrometria de Massas , Folhas de Planta/química , Hidrocarbonetos Policíclicos Aromáticos/química , Hidrocarbonetos Policíclicos Aromáticos/isolamento & purificação , Solventes , Fatores de TempoRESUMO
Organochlorines (HCH isomers, DDX and individual PCBs) were determined in pine needle litter and upper soil layers of three forest test areas in central Germany. The contents accumulating over a number of years or even decades in the organic surface layer are compared with the levels of new inputs from needle fall as well as with the levels of older inputs in the upper mineral soil layer. Differences in behaviour between the soil horizons are discussed, especially concerning the DDX and HCH groups. With approximately comparable Corg values (approx. 21-24%) the pH value in the range of 4.24-2.90 in the O-horizon of the forest soils exerts a large influence. Hence the A-horizon represents--for p,p'-DDT and gamma-HCH in particular--at pH values of 2.90 a pollutant reservoir which should not be underestimated and which could endanger the rhizosphere and the groundwater. According to PCBs, in the more acidic soils with a pH value <4.0 the lipophilic higher polychlorinated biphenyls were found to be more highly enriched in the humus layer.
Assuntos
Diclorodifenil Dicloroetileno/análise , Hexaclorocicloexano/análise , Inseticidas/análise , Pinus , Bifenilos Policlorados/análise , Poluentes do Solo/análise , Árvores , Monitoramento Ambiental , Isomerismo , Folhas de Planta/químicaRESUMO
Reversed-phase high-performance liquid chromatography (RP-HPLC) in both, isocratic and gradient elution mode (stationary phase: LiChrospher 100 RP-18; mobile phase: water/methanol or water/acetonitrile) was used for a renewed determination of octanol/water partition coefficients (Kow) of selected tetrachlorobenzyltoluene (TCBT) isomers. Reported Kow values identify this substance class as very hydrophobic but the data are relatively inconsistent. Based on a series of calibration runs with hydrophobic reference substances of different chemical structure at various eluent compositions we tested different approaches for the evaluation of isocratic retention factors (logk) and found substantial differences between the direct calibration procedure at special methanol volume fractions in the mobile phase (0.95-0.80) and the use of retention factors extrapolated to pure water as eluent (logkw). The logKow values obtained for the TCBTs with the latter approach are around 0.5 units higher and closer to literature data. The gradient elution experiments yield slightly better results compared to the isocratic direct calibration procedure, but not as good as the calibration with log kw. In addition, the use of the RP-HPLC retention factors for estimating sorption coefficients (Koc) of TCBT isomers is discussed.
Assuntos
Compostos Benzidrílicos/química , Tolueno/análogos & derivados , Tolueno/química , Calibragem , Cromatografia Líquida de Alta Pressão/métodos , Octanóis/química , Valores de Referência , Solubilidade , Água/química , Poluentes Químicos da ÁguaRESUMO
An integrative sampler that consists of a bar coated with poly(dimethylsiloxane) (PDMS) enclosed in a dialysis membrane bag has been developed combining the advantages of the passive sampling approach with solventless preconcentration of organic solutes from aqueous matrixes and subsequent desorption of the sequestered analytes on-line with a capillary GC/MS system. The performance of the sampler was tested for integrative sampling of hydrophobic persistent organic pollutants including gamma-hexachlorocyclohexane, hexachlorobenzene, 2,2'-bis(4-chlorophenyl)-1,1'-dichloroethylene, polycyclic aromatic hydrocarbons, and polychlorinated biphenyls in the laboratory in a continuous-flow system. Linear uptake of all test analytes during exposure periods up to one week has been observed, and concentration proportionality of response of the sampler has been demonstrated. Over the range of controlled laboratory conditions, the magnitude of sampling rate values varied from 47 to 700 microL h(-1) per sampler. The uptake rate of chemicals was dependent on their molecular mass, as well as on the partition coefficient between the PDMS and water. A decrease in sampling rates with decreasing water temperature was observed. The sampling device has the potential to detect low aqueous concentrations (ng to pg L(-1)) of test substances.
Assuntos
Técnicas de Química Analítica/métodos , Compostos Orgânicos/análise , Abastecimento de Água/análise , Adsorção , Técnicas de Química Analítica/instrumentação , Diálise/instrumentação , Monitoramento Ambiental/métodos , Poluentes Químicos da Água/análiseRESUMO
Concentrations of persistent organic pollutants (HCHs, DDX, PCBs and HCB) were measured in topsoils from 11 agricultural fields in the lee of large disused industrial plants in the Leipzig-Halle region at varying distances from emitters. The investigations clearly indicate the deposition of anthropogenic pollutant inputs, not only in the past, but also today. The pollution potential was assessed on the basis of current guide values and limits, as well as in relation to values found in agricultural soils elsewhere in Germany, in Europe and in the USA. The reference values were mainly exceeded for DDX and gamma-HCH (lindane). The PCB pattern was determined and the degradation ratios between the parent substances and their metabolites (DDX and HCH isomers) were calculated in order to distinguish between the previous and current pollutant input of pesticides by means of principal component analysis.
Assuntos
Agricultura , Poluentes do Solo/análise , Monitoramento Ambiental , Alemanha , Praguicidas , Valores de Referência , Poluentes do Solo/farmacocinéticaRESUMO
The reported data of biodegradability of variously substituted 25 sulfonated azo dyes were divided into two classes: 8 dyes designated "rapidly biodegradable" and 17 dyes "not rapidly biodegradable", by applying principal components analysis (PCA). The biodegradability of the dyes was modeled with the discriminant technique of two-value regression analysis based on structural descriptors. A statistically significant and biologically meaningful regression model that gives perfect classification was obtained, and the results were compared with the previous qualitative interpretation of the substituent effects on biodegradation. The coefficients of the regression model revealed a significant interaction between ring substituents for biodegradability. The results facilitate understanding of the mechanism of degradation.
Assuntos
Compostos Azo/metabolismo , Corantes/metabolismo , Modelos Teóricos , Compostos Azo/química , Biodegradação Ambiental , Corantes/química , Análise de Regressão , Poluentes Químicos da ÁguaRESUMO
Back-propagation neural network models for correlating and predicting the viscosity-temperature behavior of a large variety of organic liquids were developed. Experimental values for the liquid viscosity for 1229 data points from 440 compounds containing C, H, N, O, S, and all halogens have been collected from the literature. The data ranges covered are from -120 to 160 degrees C for temperature and from 0.164 (trans-2-pentene at 20 degrees C) to 1.34 x 10(5) (glycerol at -20 degrees C) mPa.s for viscosity value. After dividing the total database of 440 compounds into training (237 with 673 data points), validation (124 with 423 data points), and test (79 with 133 data points) sets, the modeling performance of two separate neural network models with different architectures, one based on a compound-specific temperature dependence and the second based on a compound-independent one, has been examined. The resulting former model showed somewhat better modeling performance than latter, and the model gave squared correlation coefficients of 0.956, 0.932, and 0.884 and root mean-squares errors of 0.122, 0.134, and 0.148 log units for the training, validation, and test sets, respectively. The input descriptors include molar refraction, critical temperature, molar magnetic susceptibility, cohesive energy, temperatures, and five kinds of indicator variables for functionalities, alcohols/phenols, nitriles, amines, amides, and aliphatic ring. The reliability of the proposed model was assessed by comparing the results against calculated viscosities by two existing group-contribution approaches, the method of van Velzen et al. and the Joback and Reid method.
RESUMO
Triolein-containing semipermeable membrane devices (SPMDs) were employed as passive samplers to provide data on the bioavailable fraction of organic, waterborne, organochlorine pesticides (OCPs), polychlorinated biphenyls (PCBs) and polynuclear aromatic hydrocarbons (PAHs) in streams flowing through a highly polluted industrial area of Bitterfeld in Saxony-Anhalt, Germany. The contamination of the region with organic pollutants originates in wastewater effluents from the chemical industry, from over one-hundred years of lignite exploitation, and from chemical waste dumps. The main objective was to characterise time-integrated levels of dissolved contaminants, to use them for identification of spatial trends of contamination, and their relationship to potential pollution sources. SPMDs were deployed for 43 days in the summer of 1998 at four sampling sites. The total concentration of pollutants at sampling sites was found to range from a low of 0.8 microgram/SPMD to 25 micrograms/SPMD for PAHs, and from 0.4 microgram/SPMD to 22 micrograms/SPMD for OCPs, respectively. None of the selected PCB congeners was present at quantifiable levels at any sampling site. A point source of water pollution with OCPs and PAHs was identified in the river system considering the total contaminant concentrations and the distribution of individual compounds accumulated by SPMDs at different sampling sites. SPMD-data was also used to estimate average ambient water concentrations of the contaminants at each field site and compared with concentrations measured in bulk water extracts. The truly dissolved or bioavailable portion of contaminants at different sampling sites ranged from 4% to 86% for the PAHs, and from 8% to 18% for the OCPs included in the estimation. The fraction of individual compounds found in the freely dissolved form can be attributed to the range of their hydrophobicity. In comparison with liquid/liquid extraction of water samples, the SPMD method is more suitable for an assessment of the background concentrations of hydrophobic organic contaminants because of substantially lower method quantification limits. Moreover, contaminant residues sequestered by the SPMDs represent an estimation of the dissolved or readily bioavailable concentration of hydrophobic contaminants in water, which is not provided by most analytical approaches.
Assuntos
Monitoramento Ambiental/instrumentação , Monitoramento Ambiental/métodos , Compostos Orgânicos/análise , Poluentes Químicos da Água/análise , Água Doce , Alemanha , Inseticidas/análise , Bifenilos Policlorados/análise , Hidrocarbonetos Policíclicos Aromáticos/análise , Água/químicaRESUMO
The flow patterns of 20 organic liquids with diverse structures and functionalities between electrodes were measured under a dc electric field. The results clearly showed the existence of a strong relationship between the flow pattern of a compound and its molecular structure. On the basis of a variety of 23 molecular descriptors including those obtained by quantum-chemical calculations, multiple regression analysis and discriminant analysis were applied to identify the significant factors contributing to the flow patterns. For the flow rate dipole moment, nucleophilic delocalizability and lipophilicity as expressed by the 1-octanol/water partition coefficient were found to be the key factors as judged by a five-value regression model with a squared correlation coefficient (r2) of 0.881. For the direction of the flow, just two quantum-chemical parameters, namely, absolute hardness and the self-polarizability normalized by molecular volume, were identified as significant factors by using linear discriminant analysis. The numbers of misclassified compounds were only one and two for training and prediction (leave-one-out cross-validation), respectively, by the best discriminant model.
RESUMO
Proliferation toxicity toward the algae Scenedesmus vacuolatus in a 24 h one-generation reproduction assay was determined for nitrobenzene and 18 derivatives, including two phenols. The resultant EC(50) values covering more than 4 orders of magnitude were subjected to a quantitative structure-activity analysis (QSAR) using hydrophobicity in terms of the octanol/water partition coefficient in logarithmic form, log K(ow), and 16 quantum chemical descriptors of molecular reactivity that were calculated with the AM1 scheme. For 13 mononitro derivatives and the highly hydrophobic trifluralin, a narcotic-type mode of action can explain most of the toxicity variation. Correction of log K(ow) for ionization for the phenols and quantification of the molecular susceptibility for one-electron reduction as apparently rate-determining biotransformation step by the energy of the lowest unoccupied molecular orbital, E(LUMO), yields a highly significant QSAR for all 19 compounds (r(adj)(2) = 0.90), which can be further improved when adding the maximum net atomic charge at the nitro nitrogen, q(nitro)(-)(N), as the third descriptor (r(adj)(2) = 0.93). Comparison of the energy of the singly occupied molecular orbital, E(SOMO), of the radical anions as initial metabolites with the E(SOMO) of known redox cyclers suggests that dinitrobenzenes and TFM as well as multiply chlorinated nitrobenzenes may also exert oxidative stress. This is based on an E(SOMO) window of -0.30 to 0. 55 eV as a tentative criterion for molecular structures to have the potential for redox cycling, derived from a set of eight known redox cyclers. The discussion includes a detailed analysis of apparently relevant metabolic pathways and associated modes of toxic action of nitroaromatics.
Assuntos
Chlorella/efeitos dos fármacos , Nitrobenzenos/química , Nitrobenzenos/toxicidade , Divisão Celular/efeitos dos fármacos , Chlorella/citologia , Chlorella/crescimento & desenvolvimento , Relação Dose-Resposta a Droga , Nitrobenzenos/metabolismo , Octanóis , Permeabilidade , Relação Estrutura-Atividade , Testes de Toxicidade , Trifluralina/química , Trifluralina/toxicidadeRESUMO
Systematic investigations were performed to study the dependence of the extraction efficiency of persistent organic pollutants (POPs), including chlorobenzenes, HCH isomers, DDX, PCB congeners, and PAHs, on the accelerated solvent extraction (ASE) operating variables solvent and temperature. Mixed soil samples from two locations with considerable differences in soil properties and contamination in the Leipzig-Halle region (Germany) were used. The objective was to optimize ASE for the extraction of POPs from real soil samples and to improve on the results achieved with Soxhlet extraction (SOX). Solvents with differing polarities were tested. Quadruple and triple determinations were performed on the two soils, respectively, between 20 and 180 degrees C in 20 degrees C steps. All the results were compared with those obtained by SOX, as well as, in some cases during preliminary studies, by ultrasonic extraction (USE). In ASE, the optimum conditions proved to be two extraction steps at 80 and 140 degrees C (average RSD 10.7%) with three static cycles (extraction time 35 min) using toluene as solvent and at a pressure of 15 MPa. Owing to the superior analyte/matrix separation by ASE, in many cases for real soil samples analytical values better by up to 1 order of magnitude or even more were obtained compared to SOX results.
RESUMO
A method for the detection and confirmation of heavy metal toxicants in sediment elutriates based on a urease inhibition assay, ICP-AES analysis and EDTA chelation in the frame of toxicity identification evaluation (TIE) is presented. Zinc was identified as the major toxicant in pHstat elutriates of sediments of the river Saale (Germany). Implications of natural and anthropogenic chelating agents, which are frequently present in environmental samples, on toxicity confirmation of heavy metals based on the toxic unit approach are discussed.