Feed digestibility, digesta passage and faecal microbial biomass in desert-adapted goats exposed to mild water restriction.

In arid and semi-arid environments, extensively managed ruminants regularly experience drinking water shortage, especially in the dry season. The present study therefore investigated the effects of mild drinking water restriction on feed intake, feed digestibility, solid digesta passage and composition of faeces including faecal microbial biomass. A feeding trial was conducted in Oman, during the dry summer months. Nine adult male Batinah goats were subjected to three watering regimes in a 3 × 3 Latin Square design. Treatments were (1) water offered ad libitum (100%, W100); (2) water restricted to 85% ad libitum consumption (W85); and (3) water restricted to 70% ad libitum consumption (W70). Animals were offered Rhodes grass hay and whole barley grains (1:1 ratio) at 1.3 times maintenance energy requirements. Each of the three experimental periods comprised 16 days of adaptation and 8 days of measurements. During the latter, feed offered and refused as well as faeces were sampled and quantified. Gastrointestinal digesta passage was determined using ytterbium-labelled Rhodes grass hay. Ergosterol and amino sugars were used as markers for faecal microbial biomass, that is the sum of fungi and bacteria. Water restriction had no effect on feed intake and digesta passage. However, feed dry matter, organic matter and fibre digestibility increased (p < 0.05) in W70 compared with W85, and the excreted amount of faecal dry matter, organic matter, nitrogen and neutral detergent fibre decreased (p < 0.05) in W70 compared with W85. Even though water restriction did not affect total faecal microbial biomass carbon (C) concentration, that of fungal biomass C increased (p < 0.05) in W70 compared with W85. Therefore, mild water restriction seems unproblematic from a physiological and nutrient utilization perspective as it increases feed digestibility without compromising feed intake.

Gastrointestinal nematode and Eimeria spp. infections in dairy cattle along a rural-urban gradient.

Endoparasite infections can lead to considerable economic losses in dairy cattle due to decreases in milk yield and quality. Environmental and host-related factors contribute to endoparasite infection intensity and probability. Moreover, advancing urbanization influences parasite infection dynamics in livestock due to close human-animal cohabitation and changes in animal housing conditions. The aim of the present study was to investigate social-ecological effects on gastrointestinal nematode (GIN) and Eimeria spp. infections in dairy cattle along a rural-urban gradient in the emerging Indian megacity Bangalore. In this regard, 726 faecal samples from 441 dairy cattle of different ages and physiological stages were collected from 101 farms and examined at three visits between June 2017 and April 2018. Based on a survey stratification index (SSI) comprising built-up density and distance to the city center, we assigned the farms to urban, mixed and rural areas. GIN eggs were identified in the faeces of 243 cattle (33.5%; 95% confidence interval [CI]: 30.1-36.9%), and Eimeria spp. oocysts in the faeces of 151 cattle (20.8%; 95% CI: 17.9-23.7%). Co-infection rates of GIN and Eimeria spp. were 8.5 to 12.2% higher in rural compared to urban and mixed areas. The SSI effect significantly influenced Eimeria spp. infection probability and oocyst per gram of faeces (OpG; P < 0.001) with an infection probability and OpG higher than 26% and 40% for cattle kept in rural areas compared to cattle from urban areas. However, the SSI effect was not significant for the infection probability of GIN and for GIN eggs per gram of faeces (EpG). Infection probabilities and EpG/OpG were significantly higher in calves and heifers compared to lactating and dry cows. Moreover, we estimated significantly lower OpG values in summer compared to the other seasons. No differences were estimated for GIN and Eimeria spp. infection probabilities and EpG/OpG with regard to pasture access and breed. The variations in endoparasite infection intensity and probability observed along the rural-urban gradient of Bangalore reflect the variability in dairy husbandry systems governed by the social-ecological context.

Application of the laser methane detector for measurements in freely grazing goats: impact on animals' behaviour and methane emissions.

The laser methane detector (LMD) has been increasingly used in the last decade to assess the methane (CH4) concentration in the exhaled air of ruminants. The CH4 concentration was mostly measured indoors, where animals were either retained in a feed fence or manually by a person to facilitate the LMD measurements. By contrast, the use of the LMD for measurements under outdoor conditions has been limited to very few studies. The present study applied the LMD to assess the CH4 concentration in air exhaled by four pasture-fed female Thuringian Forest goats when they were either expressing their natural grazing behaviour or when they were manually restrained at three times of the day during five consecutive days. We compared the activities, including rumination, and the CH4 concentration between restrained and free-roaming goats, and between goats, days, times of the day and goats' activities. We hypothesised that the restraint influences the goats' behaviour, particularly rumination, which could lead to a change in CH4 concentration. The overall CH4 concentration (median) was not affected by goats' restraint (6.5 ppm-m for restrained vs. 6.6 ppm-m for free-roaming). However, restraint influenced goats' rumination activity, with differences between individual goats and days. A lower rumination activity was recorded on the first and the last two days as compared to Day 2-3. Despite the greater rumination activity, the CH4 concentration was smaller on Day 2 (5.8 ppm-m) as compared to Day 5 (7.4 ppm-m). Similar observations were made with respect to behaviour and CH4 concentrations in free-roaming goats. By contrast, no differences in the proportion of time of activities were found between the times of day for restrained goats, while free-roaming goats preferred to stand idle but were less frequently lying idle in late afternoon. Still, the greatest CH4 concentration in restrained goats was obtained for the midday measurements (7.0 ppm-m), while it further increased until late afternoon for free-roaming goats (6.8 ppm-m). It is concluded that the restraint of animals during outdoor measurements can facilitate LMD measurements in grazing animals without changing the results for CH4 concentration in air exhaled by the animal. An adaptation period of one day followed by two to three measurement days with sufficient measurement periods to account for different activities is recommended to limit the impact on animals' stress level.

Processed false yam seed meals in broiler chicken diets: effects on feed preference and apparent nutrient digestibility.

This study evaluated feed preference and apparent nutrient digestibility of a maize-based diet and modified diets containing treated false yam seed meals (TFYSM). False yam seeds were sun-dried (Un_T), or first soaked in water and treated with one of the following: urea (Urea_T), sodium chloride (NaCl_T), sodium hydroxide (NaOH_T), or potassium hydroxide (KOH_T) and blanched. The TFYSM replaced maize (wt/wt) in experimental diets at 5, 10, 15, 20, 25, 30, 35, 40, 45, and 50%, respectively in a feed preference test (experiment 1). In that study, feed preference test was conducted with 6 × 4 broiler chicks aged 21 days in a randomized complete design; each level of each TFYSM was fed for 24 h and feed intake recorded. In experiment 2 of the study, apparent nutrient digestibility of TFYSM-based diets was evaluated for 15 days with 4-week-old chicks allocated to 5 groups of 20 birds which were further subdivided into 5 treatment groups of 4 birds (replicates) using the inclusion levels of 10, 20, 30, 40, and 50% respectively. The preference test data indicated that feed intake was not compromised when maize was substituted by NaCl_T, NaOH_T, Urea_T, and KOH_T FYSM at all levels, and Un_T FYSM up to 5% inclusion. Dry matter digestibility was generally high (> 75%) in all diets except for Un_T. Control diet and NaOH_T diets had similar CP digestibility, but higher than KOH_T diets, while Un_T diets had reduced CP digestibility. Digestibility of NFE ranged from (77.4%) (Un_T) to (93.8%) (KOH_T). Improving apparent nutrient digestibility of FYSM by sequential use of water-based and chemical treatment methods especially NaOH_T or KOH_T offers the potential to use this alternative feedstuff in broiler diets.

The role of cross-border transhumance in influencing resident herders' cattle husbandry practices and use of genetic resources.

For centuries, the humid West African coastal country of Benin attracts cattle herders from neighboring Sahelian countries such as Niger, Burkina Faso and Nigeria. Each year, several thousands of cattle are trekked over hundreds of kilometers to Benin following established and non-established transhumance corridors. This cross-border mobility has been shown to play an important role in ensuring the productivity of the transhumant herds through adequate late dry season access to pastoral resources. Yet, its effects on the traditional agro-pastoral production systems that are encountered along the routes in Benin have never been explicitly investigated. Therefore, we collected socio-economic household data, herd characteristics and management data from 104 resident herders and 38 transhumant herders in 2 vegetation zones of Benin. To determine whether or not the proximity to transhumance corridors affects sedentary production systems, characteristics of cattle herds and farmers' management practices were compared between villages close to (within a 25 km buffer) and far from (outside a 25 km buffer) transhumance corridors within and between vegetation zones using non-parametric statistical tests. Existing relationships between resident and transhumant herders were also identified and characterized. Subsequently, herd characteristics and management practices were compared between resident herders having relationships with transhumant herders and those without. Herd sizes of resident herders living close to transhumance corridors were larger (P < 0.01) than those of their counterparts living far away. Also, proximity to transhumance corridors had positive effects on herd management practices. The relationships between resident and transhumant herders were governed by a variety of interests including encampment/manuring contracts, exploitation of grazing lands and watering points, trading and bartering of cattle. This exchange of cattle is an important driver of change in the breed composition of local herds and represents an opportunity for resident herders to enhance their herds' productivity through crossbreeding. However, the mere replacement or indiscriminate crossbreeding of local cattle breeds with those kept by transhumant herders threatens the sustainability of the traditional resident herding systems by increasing the risk of genetic erosion and loss of valuable adaptive traits in indigenous animal genetic resources.

Severe below-maintenance feed intake increases methane yield from enteric fermentation in cattle.

The relationship between feed intake at production levels and enteric CH4 production in ruminants consuming forage-based diets is well described and considered to be strongly linear. Unlike temperate grazing systems, the intake of ruminants in rain-fed tropical systems is typically below maintenance requirements for part of the year (dry seasons). The relationship between CH4 production and feed intake in animals fed well below maintenance is unexplored, but changes in key digestive parameters in animals fed at low levels suggest that this relationship may be altered. We conducted a study using Boran yearling steers (n 12; live weight: 162·3 kg) in a 4 × 4 Latin square design to assess the effect of moderate to severe undernutrition on apparent digestibility, rumen turnover and enteric CH4 production of cattle consuming a tropical forage diet. We concluded that while production of CH4 decreased (1133·3-65·0 g CH4/d; P < 0·0001), over the range of feeding from about 1·0 to 0·4 maintenance energy requirement, both CH4 yield (29·0-31·2 g CH4/kg DM intake; P < 0·001) and CH4 conversion factor (Ym 9·1-10·1 MJ CH4/MJ gross energy intake; P < 0·01) increased as intake fell and postulate that this may be attributable to changes in nutrient partitioning. We suggest there is a case for revising emission factors of ruminants where there are seasonal nutritional deficits and both environmental and financial benefits for improved feeding of animals under nutritional stress.

Effects of quebracho tannin extract and activated charcoal on nutrient digestibility, digesta passage and faeces composition in goats.

Irrigation agriculture of hot climates suffers from rapid soil organic carbon (C) turnover, high nitrogen (N) leaching and gaseous losses. In this context, we explored the possibility of supplementing ruminants with activated charcoal (AC) and condensed tannins (CT) to increase faecal concentration of long-lived C and slowly decomposable N. For this purpose, we fed AC and quebracho tannin extract (QTE, source of CT) to goats and determined nutrient digestibility, digesta passage and composition of faeces. The feeding trial comprised three periods, with 24 male goats assigned to five treatments and a control. On dry matter (DM) basis, the basal diet (control) consisted of 50% hay, 46.5% crushed maize and 3.5% soya bean meal. Treatment diets contained (on DM basis) 2% and 4% QTE (QTE2, QTE4), 1.5% and 3.0% AC (AC1.5, AC3.0) and a mixture of 2% QTE plus 1.5% AC (QTEAC). Samples of feed, refusals and faeces were analysed for chemical constituents; digesta passage was computed with a one-compartment Gamma-2 model from pulse-dose application of ytterbium-mordanted fibre and quantitative collection of faeces during 7 days. Compared to the control, organic matter and N digestibility were reduced with QTE2 and QTE4 (p ≤ 0.001), that of neutral and acid detergent fibre (NDFom, ADFom) decreased with QTE4, QTEAC and both AC treatments (p ≤ 0.001). Ruminal particle passage ranged from 0.051 h-1 (QTEAC) to 0.056 h-1 (control; p > 0.05). Apart from the correlation between total tract mean retention time and faecal C/N ratio (r = -0.35), passage parameters did not affect faeces quality. While faecal N concentration was not altered by both additives, AC enhanced faecal NDFom, ADFom and C concentrations (p ≤ 0.001). Whereas feeding up to 3% AC might slow down the decomposition of manure in the field without negatively affecting the digestive physiology of goats, including QTE seems not advantageous from a physiological and nutrient recycling perspective.

Towards conservation of omani local chicken: phenotypic characteristics, management practices and performance traits.

Characterizing local chicken types and their mostly rural production systems is prerequisite for designing and implementing development and conservation programs. This study evaluated the management practices of small-scale chicken keepers and the phenotypic and production traits of their chickens in Oman, where conservation programs for local livestock breeds have currently started. Free-range scavenging was the dominant production system, and logistic regression analysis showed that socio-economic factors such as training in poultry keeping, household income, income from farming and gender of chicken owners influenced feeding, housing, and health care practices (p<0.05). A large variation in plumage and shank colors, comb types and other phenotypic traits within and between Omani chicken populations were observed. Male and female body weight differed (p<0.05), being 1.3±0.65 kg and 1.1±0.86 kg respectively. Flock size averaged 22±7.7 birds per household with 4.8 hens per cock. Clutch size was 12.3±2.85 and annual production 64.5±2.85 eggs per hen. Egg hatchability averaged 88±6.0% and annual chicken mortality across all age and sex categories was 16±1.4%. The strong involvement of women in chicken keeping makes them key stakeholders in future development and conservation programs, but the latter should be preceded by a comprehensive study of the genetic diversity of the Omani chicken populations.

Adjusting homestead feeding to requirements and nutrient intake of grazing goats on semi-arid, subtropical highland pastures.

Intensive livestock grazing can largely deplete the natural fodder resources in semi-arid, subtropical highlands and together with the low nutritional quality of the pasture vegetation limit the growth and production of grazing animals. To evaluate the contribution of homestead feeding of grazing goats to rangeland conservation and animal nutrition, two researcher-managed on-farm trials were conducted in a mountain oasis of Northern Oman. Goats' feed intake on pasture in response to four rations containing different levels of locally available green fodder and concentrate feeds was determined in six male goats each (35 ± 10.2 kg body weight (BW)). Total feed intake was estimated using titanium dioxide as external fecal marker as well as the diet organic matter (OM) digestibility derived from fecal crude protein concentration. The nutritional quality of selected fodder plants on pasture was analyzed to determine the animals' nutrient and energy intake during grazing. The pasture vegetation accounted for 0.46 to 0.65 of the goats' total OM intake (87 to 107 g/kg0.75 BW), underlining the importance of this fodder resource for the husbandry system. However, metabolizable energy (7.2 MJ/kg OM) and phosphorus concentrations (1.4 g/kg OM) in the consumed pasture plants were low. Homestead feeding of nutrient and energy-rich by-products of the national fishery and date palm cultivation to grazing goats increased their daily OM intake (R2 = 0.36; P = 0.005) and covered their requirements for growth and production. While the OM intake on pasture was highest in animals fed a concentrate-based diet (P = 0.003), the daily intake of 21 g OM/kg0.75 BW of cultivated green fodder reduced the animals' feed intake on pasture (R2 = 0.44; P = 0.001). Adjusting homestead supplementation with locally available feedstuffs to the requirements of individual goats and to the nutritional quality of the pasture vegetation improves animal performance and eases the grazing pressure exerted on the natural vegetation. This management strategy therefore appears to be a valuable alternative to intensive livestock feeding in zero-grazing systems and may contribute to sustainable livestock production in ecologically fragile, semi-arid mountain regions.

Resource use efficiency in urban and peri-urban sheep, goat and cattle enterprises.

Urban livestock husbandry receives growing attention given the increasing urban demand for livestock products. At the same time, little is known about the resource use efficiency in urban livestock enterprises and eventual negative externalities. In livestock production, feeds are an important resource whose nutrients are transformed into products (meat and milk) to generate financial return to the producer. The lack of knowledge on nutrient supply through feed might lead to oversupply with severe environmental impacts. In Niamey, a typical West African city and capital of the Republic of Niger, urban livestock production is constrained by feed scarcity, especially during the dry season. Here, the issue of resource use efficiency was studied in 13 representative and differently managed sheep/goat and cattle enterprises characterized by high and low feed inputs, respectively, during a period of 28 months. Nitrogen (N), phosphorus (P) and potassium (K) inflows into each farm through livestock feeds and outflows through manure were determined using a semi-structured questionnaire; interviews were accompanied by regular weighing of feed supplied and dung produced. Live weight gain (LWG) and efficiency of conversion of total feed dry matter offered (kg TDMO/kg LWG) were computed along with nutrient balances (NBs) per metabolic body mass (kg0.75). NBs (per kg0.75/day) in the high-input (HI) sheep/goat enterprises were +1762.4 mg N, +127.2 mg P and +1363.5 mg K and were significantly greater (P < 0.05) than those in low-input (LI) units (+69.1 mg N, -98.3 mg P and +16.5 mg K). In HI cattle enterprises, daily balances averaged +454.1 mg N, +40.1 mg P and +341.8 mg K compared to +34.4 mg N, -9.0 mg P and +68.3 mg K (P > 0.05) in LI cattle systems. All systems were characterized by poor conversion efficiencies of offered feed, which ranged from 13.5 to 46.1 kg TDMO/kg LWG in cattle and from 15.7 to 43.4 kg TDMO/kg LWG in sheep/goats. LWG in HI sheep/goats was 53 g/day in the rainy season, 86 g/day in the hot dry season and 104 g/day in the cool dry season, while HI cattle lost 79 g/day in the hot dry season and gained 121 g/day and 92 g/day in the cool dry and rainy seasons, respectively. The data indicate that there is nutrient wasting and scope for improvement of feeding strategies in Niamey's livestock enterprises, which might also decrease nutrient losses to the urban environment.

Estimating the digestibility of Sahelian roughages from faecal crude protein concentration of cattle and small ruminants.

Studies on diet selection and feed intake of ruminants in extensive grazing systems often require the use of simple approaches to determine the organic matter digestibility (OMD) of the ingested feed. Therefore, we evaluated the validity of the one-factorial exponential regression established by Lukas et al. [Journal of Animal Science 83 (2005) 1332], which estimates OMD from the faecal crude protein (FCP) concentration. The equation was applied to two sets of data obtained with free grazing and pen-fed cattle, sheep and goats ingesting low and high amounts of green and dry vegetation of Sahelian pastures as well as millet leaves and cowpea hay. Data analysis showed that the livestock species did not influence the precision of estimation of OMD from FCP. For the linear regression between measured and estimated OMD (%) across n = 431 individual observations, a regression coefficient of r2 = 0.65 and a residual standard deviation (RSD) of 5.87 were obtained. The precision of estimation was influenced by the data set (p = 0.033), the type of feed (p < 0.001) and the feeding level (p = 0.009), and interactions occurred between type of feed and feeding level (p = 0.021). Adjusting the intercept and the slope of the established exponential function to the present data resulted in a compression of the curve; while r2 remained unchanged, the RSD of the regression between measured and estimated OMD was reduced, when compared with the results obtained from the equation of Lukas et al. (2005). Estimating OMD from treatment means of FCP greatly improved the correlation between measured and estimated OMD for both the established function and the newly fit equation. However, if anti-nutritional dietary factors increase the concentration of faecal nitrogen from feed or endogenous origin, the approach might considerably overestimate diet digestibility.

Granulated polyamide as external marker to estimate total faecal excretion of grazing cattle in extensive management systems.

Granulated polyamide (PA) was tested for use as an external marker to estimate faecal DM (FDM) excretion of Zebu cattle (Bos indicus). The study was conducted in Mali, using seven and eighteen animals respectively in four field trials and six indoor experiments. Cattle ate fresh or dry pasture vegetation and half the animals were additionally supplemented with crop byproducts. Gelatine capsules containing 35, 40 or 45 g PA were administered orally at 12 h intervals. Estimates of FDM were based on the average marker concentration in faeces and were correlated with the actual excretion measured by total faecal collection. The pre-measurement period required to establish equilibrium for regular marker dosing was determined at 4 d. Except for diets with a N content of less than 9.26 g/kg organic matter, marker recovery averaged 98.1 (SE 0.93)% (n 62), and was not influenced by diet composition and the quantity of feed ingested (P > 0.05). Estimates of FDM based on average PA concentrations in faecal samples were correlated to the actual excretion with r 0.98 (n 62; P < or = 0.001). Since the PA concentration in individual faecal grab-samples is not correlated with either sample mass or sampling time, accurate estimates of FDM require a grab-sampling schedule that covers the 24 h day. However, estimates of FDM were found to be acceptable if calculations are based on the average PA concentration in the sub-total of samples collected during the day or during night respectively (r 0.95, n 29; P < or = 0.001 in both cases). It is concluded that the use of PA marker is a simple and inexpensive method resulting in reliable estimates of FDM. Since sophisticated analytical procedures are not required to recover PA in faecal samples, the marker is particularly suitable for application in extensive grazing systems and in studies conducted in less-developed countries.