RESUMO
OBJECTIVE: The aim of this study was to compare the endometrial expression of milk fat globule-EGF factor 8 (MFG-E8), its receptor integrin αvß3, and leukemia inhibitory factor (LIF) in patients with endometriosis and infertility and in healthy fertile patients during the window of implantation. METHODS: Five patients with peritoneal endometriosis and infertility (case group) and four healthy fertile patients (control group) were recruited. All patients were either diagnosed with or ruled out for endometriosis by laparoscopic surgery; the case group underwent surgery for infertility investigation and the control group for tubal ligation. Endometrial biopsies were performed in all patients during the window of implantation (LH+8 to LH+10), and then the samples were analyzed by immunochemistry for MFG-E8, integrin αvß3, and LIF. RESULTS: In patients with endometriosis and infertility, expression of MFG-E8 was significantly increased in the glandular epithelium when compared to healthy fertile patients (p<0.001). Moreover, LIF expression was lower in patients with endometriosis and infertility (p<0.05). Nevertheless, we found no difference in integrin αvß3 expression between the groups (p=0.084). CONCLUSION: This study showed for the first time that MFG-E8 expression is impaired in the endometrium of patients with endometriosis and infertility during the window of implantation. Moreover, LIF is also diminished in the endometrium of these patients as shown before.
Assuntos
Antígenos de Superfície/metabolismo , Endometriose/metabolismo , Endométrio/metabolismo , Infertilidade Feminina/metabolismo , Fator Inibidor de Leucemia/metabolismo , Proteínas do Leite/metabolismo , Doenças Peritoneais/metabolismo , Adulto , Estudos de Casos e Controles , Endometriose/patologia , Endométrio/patologia , Feminino , Fertilidade/fisiologia , Humanos , Infertilidade Feminina/patologia , Integrina alfaVbeta3/metabolismo , Doenças Peritoneais/patologia , Estudos ProspectivosRESUMO
PURPOSE: To verify if polymorphisms of LH (Trp8Arg/Ile15Thr), LH receptor (insLQ), and FSH receptor (Asn680Ser) are associated with endometriosis and infertility. METHODS: This is a prospective case-control study. Sixty-seven patients with endometriosis and infertility (study group) and 65 healthy fertile patients (control group) were enrolled in the study between July 2010 and July 2013. All patients had their endometriosis diagnosis made or excluded by laparoscopic surgery; study group was submitted to the surgery for infertility investigation and control group for tubal ligation. Day-3 serum hormones were collected from all patients. Analysis of nucleotide mutations for LH polymorphisms (Trp8Arg and Ile15Thr), LHR polymorphism (insLQ), and FSHR polymorphism (Asn680Ser) were performed by PCR. RESULTS: Day-3 FSH, estradiol and LH serum levels were not different between the groups, while CA-125 was higher in patients with endometriosis and infertility. All polymorphisms studied were in Hardy-Weinberg equilibrium. The prevalence of insLQ was significantly higher in patients with endometriosis and infertility (P = 0.005). Allele occurrence in control group was 0.10 versus 0.25 in infertile endometriosis group (P = 0.001). There was no difference regarding Trp8Arg/Ile15Thr (P > 0.05) and Asn680Ser (P > 0.05) prevalence between groups. CONCLUSION: This is the first time that prevalence of insLQ was shown to be higher in patients with endometriosis and infertility than in healthy fertile patients. There was no difference in LH and FSHR polymorphisms' prevalence between groups.
Assuntos
Endometriose/genética , Infertilidade Feminina/genética , Hormônio Luteinizante/genética , Polimorfismo Genético , Receptores do FSH/genética , Receptores do LH/genética , Adulto , Estudos de Casos e Controles , Endometriose/complicações , Feminino , Humanos , Infertilidade Feminina/complicações , Hormônio Luteinizante/química , Análise Multivariada , Estudos Prospectivos , Receptores do FSH/química , Receptores do LH/químicaRESUMO
Amenorréia é a ausência ou parada anormal de sangramento menstrual na menacme; para conduzir sua investigação, é importante descartar causas fisiológicas (gravidez e puerpério). além disso, deve-se conhecer as diversas situações patológicas que se manifestam como amenorréia, as quais podem ocorrer por disfunção do eixo hipotálamo-hipófise-gonadal o a anatomia do trato genital. nesse artigo serão revisadas as principais etiologias da amenorréia e sua investigação.
Assuntos
Humanos , Feminino , Amenorreia , Amenorreia/etiologiaRESUMO
Os autores fazem uma revisão bibliográfica sobre endometriose associada à infertilidade com enfoque aos aspectos terapêuticos da doença.