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PLoS One ; 7(9): e43879, 2012.
Artigo em Inglês | MEDLINE | ID: mdl-22984449

RESUMO

BACKGROUND: We recently characterized a specific inorganic triphosphatase (PPPase) from Nitrosomonas europaea. This enzyme belongs to the CYTH superfamily of proteins. Many bacterial members of this family are annotated as predicted adenylate cyclases, because one of the founding members is CyaB adenylate cyclase from A. hydrophila. The aim of the present study is to determine whether other members of the CYTH protein family also have a PPPase activity, if there are PPPase activities in animal tissues and what enzymes are responsible for these activities. METHODOLOGY/PRINCIPAL FINDINGS: Recombinant enzymes were expressed and purified as GST- or His-tagged fusion proteins and the enzyme activities were determined by measuring the release of inorganic phosphate. We show that the hitherto uncharacterized E. coli CYTH protein ygiF is a specific PPPase, but it contributes only marginally to the total PPPase activity in this organism, where the main enzyme responsible for hydrolysis of inorganic triphosphate (PPP(i)) is inorganic pyrophosphatase. We further show that CyaB hydrolyzes PPP(i) but this activity is low compared to its adenylate cyclase activity. Finally we demonstrate a high PPPase activity in mammalian and quail tissue, particularly in the brain. We show that this activity is mainly due to Prune, an exopolyphosphatase overexpressed in metastatic tumors where it promotes cell motility. CONCLUSIONS AND GENERAL SIGNIFICANCE: We show for the first time that PPPase activities are widespread in bacteria and animals. We identified the enzymes responsible for these activities but we were unable to detect significant amounts of PPP(i) in E. coli or brain extracts using ion chromatography and capillary electrophoresis. The role of these enzymes may be to hydrolyze PPP(i), which could be cytotoxic because of its high affinity for Ca(2+), thereby interfering with Ca(2+) signaling.


Assuntos
Bactérias/enzimologia , Compostos Inorgânicos/metabolismo , Mamíferos/metabolismo , Fosfoproteínas Fosfatases/metabolismo , Trifosfato de Adenosina/metabolismo , Adenilil Ciclases/metabolismo , Sequência de Aminoácidos , Animais , Biocatálise , Sobrevivência Celular , Eletroforese Capilar , Escherichia coli/enzimologia , Proteínas de Escherichia coli/química , Proteínas de Escherichia coli/metabolismo , Hidrólise , Pirofosfatase Inorgânica/metabolismo , Dados de Sequência Molecular , Nitrosomonas europaea/enzimologia , Fosfoproteínas Fosfatases/química , Polifosfatos/isolamento & purificação , Polifosfatos/metabolismo , Ratos , Proteínas Recombinantes/metabolismo , Alinhamento de Sequência , Frações Subcelulares/enzimologia , Especificidade por Substrato
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