Air-classified faba bean protein fraction as a substitute to soybean meal in pelleted and extruded broiler diets.

1. The hypothesis that air-classified faba bean protein fraction (FBP) can replace soybean meal (SBM) in pelleted or extruded broiler diets without adverse effect on performance or nutrient digestibility was tested.2. At 17 d of age, male broilers were randomly distriibuted among four dietary treatments consisting of either SBM or FBP (main dietary protein source) and pelleting or extrusion as processing methods. Treatments had 10 replicate pens containing five birds each.3. Compared to SBM, birds fed FBP had significantly lower feed intake, less weight gain and had poorer feed conversion.4. Pellet durability was high (above 92%) for all diets. In pelleted diets, FBP was harder than SBM whereas extruded diets had similar hardness. Pelleting increased water stability compared to extrusion. FBP diets were more water stable than the SBM diets.5. Gizzard content weight was 2.2-fold higher (P = 0.002) for birds given FBP compared to those fed SBM. The weight of the jejunum and ileum with contents was 1.4-fold lower for the FBP diets, and this effect was larger (P < 0.05) for the extruded FBP diet.6. Birds fed FBP diets had significantly higher nitrogen digestibility in the lower jejunum and ileum compared to those given SBM diets, while the starch digestibility coefficient was above 0.980 in all treatments.7. The high nutrient digestibility of FBP diets indicates that the poor performance of the FBP group was due to lower feed intake which was not explained by the differences in pellet durability or hardness. The reduced palatability of the FBP and the longer retention of the FBP diets in the upper gut are more likely to have depressed feed intake.

[Sectio caesarea: actual controversy]. / Sectio caesarea: Aktuelle Kontroversen.

Caesarean section is one of the most frequently performed operations in human medicine. It has become a routine procedure with a very low morbidity and mortality. Over the centuries, it has emerged an essential achievement in obstetric medicine. In the presence of cephalo-pelvic-disproportion, cervical dystocia, malpresentation, preterm birth, macrosomia, placental insufficiency, placenta praevia or fetal distress it is crucial to improve the perinatal morbiditiy and mortality of mother and child. The procedure has become much more frequent over the past 20 years for multiple reasons. There is variety in incidence between countries but also regional differences. It's occurrence is being influenced by level of education and socio-economic status of the pregnant woman. In the meantime, also the longterm consequences of cesarean section are well known. The potential hazards for future pregnancies and deliveries are well described. Actual controversy addresses cesarean section on demand without any medical indication, which in fact is only seldom performed in Switzerland. The ethical justification of this procedure needs to be discussed in view of the current tendency towards autonomy and self-determination of the pregnant woman.

A novel bispecific EGFR/Met antibody blocks tumor-promoting phenotypic effects induced by resistance to EGFR inhibition and has potent antitumor activity.

Simultaneous targeting of epidermal growth factor receptor (EGFR) and Met in cancer therapy is under pre-clinical and clinical evaluation. Here, we report the finding that treatment with EGFR inhibitors of various tumor cells, when stimulated with hepatocyte growth factor (HGF) and EGF, results in transient upregulation of phosphorylated AKT. Furthermore, EGFR inhibition in this setting stimulates a pro-invasive phenotype as assessed in Matrigel-based assays. Simultaneous treatment with AKT and EGFR inhibitors abrogates this invasive growth, hence functionally linking signaling and phenotype. This observation implies that during treatment of tumors a balanced ratio of EGFR and Met inhibition is required. To address this, we designed a bispecific antibody targeting EGFR and Met, which has the advantage of a fixed 2:1 stoichiometry. This bispecific antibody inhibits proliferation in tumor cell cultures and co-cultures with fibroblasts in an additive manner compared with treatment with both single agents. In addition, cell migration assays reveal a higher potency of the bispecific antibody in comparison with the antibodies' combination at low doses. We demonstrate that the bispecific antibody inhibits invasive growth, which is specifically observed with cetuximab. Finally, the bispecific antibody potently inhibits tumor growth in a non-small cell lung cancer xenograft model bearing a strong autocrine HGF-loop. Together, our findings strongly support a combination treatment of EGFR and Met inhibitors and further evaluation of resistance mechanisms to EGFR inhibition in the context of active Met signaling.

External quality assessment of human neutrophil antigen (HNA)-specific antibody detection and HNA genotyping from 2000 to 2012.

Since 2000, Quality Assurance (QA) exercises for the detection and identification of granulocyte antibodies and DNA typing for human neutrophil antigens (HNA) have been distributed within the International Granulocyte Immunobiology Workshops, which are linked to International Society of Blood Transfusion. The exercises were standardised at the outset to enable laboratory performance to be monitored. Between 2000 and 2012, nine exercises were distributed to 20 laboratories. Overall, 45 examples of 42 unique samples containing defined granulocyte reactive antibodies were distributed for serological analysis together with 20 samples for HNA genotyping. The level of satisfactory serological performance was initially set at 50% and later increased to 70%, while the 'cut-off' for HNA genotyping was set at 100% after 2008. Failure to achieve the minimum score in the QA exercises in consecutive years resulted in temporary exclusion. In 2000, the 15 participating laboratories had a mean score of 56.1% for serological analysis and 13 laboratories attempted HNA-1a and -1b genotyping, while 11 attempted HNA-1c typing. Steady improvements in proficiency for serological testing and HNA typing occurred in subsequent exercises. In 2012, the mean score for serology was 88.5% and 12/13 laboratories scored 100% for HNA-1a, -1b, -1c, -3a, -3b, -4a, -4bw, -5a and -5bw genotyping. These QA exercises have provided an invaluable tool to monitor and improve the standard of granulocyte immunology investigations for participating laboratories, thereby enhancing performance for both clinical investigations and donor screening programmes to reduce the incidence of TRALI.

Constant and variable amplitude ultrasonic fatigue of 2024-T351 aluminium alloy at different load ratios.

Ultrasonic fatigue testing equipment is presented that is capable of performing constant amplitude (CA) and variable amplitude (VA) experiments at different constant load ratios. This equipment is used to study cyclic properties of aluminium alloy 2024-T351 in the high cycle fatigue (HCF) and very high cycle fatigue (VHCF) regime at load ratios R=-1 and R=0.5. CA loading does not reveal a fatigue limit below 10¹° cycles. Cracks leading to VHCF failure start at broken constituent particles. Specimens that survived more than 10¹° cycles at R=-1 contain non-propagating cracks of lengths below grain size. Resonance frequency and nonlinearity parameter ß(rel) show changes of vibration properties of specimens at low fractions of their VHCF lifetime. VA lifetimes are measured in the HCF and VHCF regime and compared with Miner calculations. Damage sums decrease with decreasing load (and increasing mean lifetimes) and are lower for R=0.5 than R=-1.

A standardized immunofluorescence test method with human neutrophil antigen-expressing cell lines to enhance antibody detection.

There is an international need for a large-scale human neutrophils antigen (HNA) antibody screening platform to minimize the risk of antibody-mediated transfusion-related acute lung injury. However, sourcing a substantial, reliable source of HNA, as well as the scarcity of well-characterized HNA antisera for validating new screening platforms, remain as major obstacles. This short communication presents an improved protocol for the effective use of HNA-expressing KY cells as a screening platform using eight well-characterized HNA antisera of a single defined specificity.

Granulocyte serology: current concepts and clinical signifcance.

Applying serologic procedures to the detection of RBC and lymphocyte antigens has facilitated the identification of granulocyte antigens with established clinical significance, which are now classified in the human neutrophil antigen system. Granulocyte alloantibodies and autoantibodies have been implicated in a variety of clinical conditions including alloimmune neutropenia, autoimmune neutropenia, febrile and severe pulmonary transfusion reactions, drug-induced neutropenia, refractoriness to granulocyte transfusions, and immune neutropenia after hematopoietic stem cell transplantation. Although the intrinsically fragile nature of granulocytes contributes to the inherent challenges of granulocyte serology, several advances in laboratory procedures have improved detection of granulocyte antibodies. This review will provide a current perspective about the importance and use of granulocyte serology for detection of granulocyte antibodies that have significant medical effects.

Recommendations of the ISBT Working Party on Granulocyte Immunobiology for leucocyte antibody screening in the investigation and prevention of antibody-mediated transfusion-related acute lung injury.

BACKGROUND: Transfusion-related acute lung injury (TRALI) is currently one of the most common causes of transfusion-related major morbidity and death. Among the many TRALI mediators, leucocyte antibodies have been identified as important triggers of severe TRALI. STUDY DESIGN AND METHODS: These recommendations were compiled by experts of the ISBT Working Party on Granulocyte Immunobiology, based on the results obtained in eight international granulocyte immunology workshops, their personal experiences and on published study results. RESULTS: Leucocyte antibody screening has to include the detection of human leucocyte antigen (HLA) class I, class II and human neutrophil alloantigen antibodies using established and validated techniques. HLA class I antibody detection should be restricted to antibodies clinically relevant for TRALI. To avoid unnecessary workload, TRALI diagnosis should be assessed by consultation with the reporting clinician and thorough exclusion of transfusion-associated circulatory overload/cardiac insufficiency. In patients diagnosed with TRALI having donors with detectable leucocyte antibodies, evidence of leucocyte incompatibility should be provided by either cross-matching or typing of patient for cognate antigen. CONCLUSION: Leucocyte antibody screening for the immunological clarification of TRALI cases as well as for identification of potentially alloimmunized blood donors is feasible and can be performed in a reasonable and quality assured manner. This practice can contribute to the prevention of antibody-mediated TRALI.

In vitro release of curcumin from vehicles containing alginate and cyclodextrin. Studies of curcumin and curcuminoides. XXXIII.

Combinations of cyclodextrins and alginates were used to solubilize the hydrophobic compound curcumin in aqueous vehicles intended for topical delivery. A careful selection of the excipients is necessary to achieve a sufficient release of curcumin towards a membrane of hydrophilic character, e.g. mucosa. The aim of the study was to investigate the effect of different combinations of cyclodextrins and alginates on curcumin release towards hydrophilic membranes in vitro. The curcumin flux through semi-permeable membranes of different molecular weight cut-off was measured to differentiate between the flux of curcumin in its uncomplexed form (restricted flux), its uncomplexed form together with its inclusion complexes (partly restricted flux) and the overall flux of curcumin-cyclodextrin complexes and uncomplexed curcumin (unrestricted flux). A high viscosity of the vehicle was expected to inhibit curcumin flux. Vehicles containing 3% alginate were found to have lower unrestricted flux than the vehicles containing 0.5% alginate independent of the type of cyclodextrin used. The results indicate that the unrestricted curcumin flux (e.g. in the case of wounded skin) is rather independent of the composition of the hydrophilic vehicle and mostly limited by the viscosity. However, partly restricted and restricted curcumin flux were found to depend on both the viscosity and the composition of the vehicles. The cyclodextrin with the demonstrated lowest solubilisation capacity (i.e. hydroxypropyl-beta-cyclodextrin) resulted in the highest values of both the restricted and unrestricted curcumin flux. In conclusion, a combination of hydroxypropyl-beta-cyclodextrin and propylene glycol alginate seemed to be the best choice with respect to curcumin solubility and release from the vehicle.

Patient-specific transfusion-related acute lung injury.

Transfusion-related acute lung injury (TRALI) is one of the leading causes of transfusion-associated mortality. The inadvertent transfusion of neutrophil antibodies can cause pulmonary transfusion reactions and TRALI. However, not all patients transfused with neutrophil antibodies experience transfusion reactions. A 22-year-old man with severe aplastic anaemia (SAA) experienced TRALI after a platelet transfusion. The donor was found to be alloimmunized to human neutrophil antigen (HNA)-3a, an antigen expressed by neutrophils from approximately 90% of Caucasians. Eleven other platelet components from this donor were transfused prior to this event and two caused reactions: one chills and one TRALI. Both episodes of TRALI occurred in the same male patient with SAA. The fact that one patient experienced TRALI following both exposures to anti-HNA-3a from the same donor whereas nine other recipients did not adds evidence to the observation that patient factors make a significant contribution to neutrophil antibody-mediated transfusion reactions.

Police responses to sexual assault complaints: the role of perpetrator/complainant intoxication.

This study explored the impact of victim and perpetrator alcohol consumption on police officers' evaluations of an alleged sexual assault and their reported likelihood of charging the perpetrator. Two hundred and twelve police officers were presented with a vignette depicting an acquaintance rape in which the beverage consumption (beer, cola) of both the victim and perpetrator was systematically varied. Results indicated that the officers' perceptions of the complainant's intoxication level, as well as the gender of the officer, influenced officers' evaluations of the alleged sexual assault. The more intoxicated the complainant was perceived to be, the more negatively she was viewed. Female police officers evaluated the victim more favorably than male officers. The only factors related to the officers' likelihood of charging the perpetrator, however, involved their assessment of the complainant's credibility and their perception of the likelihood that the perpetrator would be found guilty in a court of law.

Expert evidence pertaining to battered women: the impact of gender of expert and timing of testimony.

The present study investigated the impact of two variables relating to general expert testimony pertaining to battered women on juror decision processes. Specifically, the gender of the expert, as well as the timing of the presentation of the testimony, were investigated in a simulated homicide trial involving a battered woman who had killed her abuser. Results indicated that when the expert was female and the testimony was presented prior to the defendant's testimony, juror's verdicts were more lenient. Moreover, across a range of case judgments, male jurors' perceptions were more favorable to the defendant when the expert was female as opposed to male. On two of the judgments this latter pattern of results was only evidenced when the testimony was presented early as opposed to late. The significance of these findings is discussed.

Peer group training of pragmatic skills in adolescents with acquired brain injury.

OBJECTIVE: To empirically evaluate a method of treating adolescents with cognitive communication disorders, including pragmatic deficits, secondary to acquired brain injury (ABI) in a group setting by objectively measuring outcomes before treatment and immediately after treatment and at 6 months posttreatment. DESIGN: A before-after trial with follow-up in a consecutive sample, with no control group. SETTING: Inpatient and outpatient pediatric rehabilitation center. SUBJECTS: Adolescents who demonstrated pragmatic deficits and scored a rating of 3 or less on each subdomain of the Rehabilitation Institute of Chicago Rating Scale of Pragmatic Communication Skills (RICE-RSPCS) were eligible for the study. Eight subjects were recruited into the study, and two subjects were lost to follow-up. Thus, six of the eight completed the study. MAIN OUTCOME MEASURES: RICE-RSPCS, Communication Performance Scale (CPS). RESULTS: Clinically relevant and statistically significant (P <.01) changes occurred during the treatment and were maintained at follow-up for the four RICE-RSPCS subscales and the CPS. CONCLUSION: These results suggest that the potential and often typical long-term pragmatic and subsequent social difficulties associated with ABI can possibly be lessened through effective intervention.

An interface to enhance mobility for people with hemispatial neglect.

The objective was to develop and test a control interface to enable people with hemispatial neglect to maneuver a powered wheelchair with increased attention to their environment. A single case study was conducted using a female (aged 72 years) who had a right cerebro vascular accident five years earlier. She drove a powered wheelchair through 20 different test courses (mazes) while a computer recorded frequency of looking to the left (left checks) and an observer recorded visual targets missed and wheelchair collisions with walls and obstacles. The Mann-Whitney test was used to determine significant differences in left checks, left collisions, right collisions, and percent left checks targets missed between baseline and strategy phases. With the interface strategy, the subject significantly increased the number of left checks and missed fewer left targets in the test course. These results indicate that a powered-wheelchair control interface shows promise in enabling people with mild hemispatial neglect to maneuver a powered chair with greater environmental awareness and ease.

Outcomes measurement of assistive technologies: an institutional case study.

The assistive technology (AT) community has been challenged to effectively measure the outcomes of AT services. There has been much discussion recently in the literature about how to conceptualize and respond to this challenge. In this paper, we suggest that these objectives are best accomplished when AT services are understood within the contexts of the total rehabilitation of AT users and the institutional culture in which services are delivered. We provide examples of outcome priorities we have identified and the tools and approaches we have used. These include projects in the areas of clinical, functional, and psychosocial outcomes assessment of ATs.

Aging and attitude change.

Social psychologists have proposed a variety of different models to account for people's relative openness to attitude change through the life cycle. Two of the most important models are the impressionable years model, which suggests an especially great openness to change among the young, and the lifelong openness model, which suggests that age is unrelated to openness to attitude change. Two studies were conducted to examine the openness of people of varying ages to attitude change. In both studies, the influence of personal experiences with government agencies on attitudes toward government was examined. The attitudes of older people changed as much or more in response to their personal experiences as did those of younger people. These results support the lifelong openness model of attitude change.

A procedure for reducing errors in reports of life events.

Despite the widespread use of life event inventories, relatively little research has been conducted evaluating whether recall techniques can reduce reporting errors. This study evaluated whether memory aids would reduce errors in reports of life events and explored subjects' explanations for why they had given inconsistent answers to life event questions over the course of two interviews. Seventy-one college students were randomly assigned to two groups: memory aids (MA: n = 36) and no memory aids (NMA: n = 35). Memory aids, as used in this study, reduced errors in reports of life events as reflected in the following results: (1) significantly fewer subjects in the MA group reported dating events incorrectly; (2) subjects in the MA group consistently gave more reliable responses between the two interviews as measured by intraclass correlations; (3) there was a significantly higher test-retest reliability correlation for life events reported by MA subjects; and, (4) MA subjects recalled more life events and had fewer inconsistent responses. A further probe revealed that memory aids differentially aided recall of subjective as compared to objective life events. Overall, the findings of this study suggest that memory aid procedures can differentially reduce errors in reporting life events.

Predicting discharge and follow-up status of hospitalized adolescents.

The functioning of 58 adolescent psychiatric patients at hospital admission, discharge, and one-and-a-half-year follow-up was examined in relation to six predictor variables: level of precipitating stress, primary process thinking on the Rorschach test, cognitive inefficiency, severity of psychopathology, maladaptive behavior on the unit, and process versus reactive illness. Severity of psychopathology and cognitive inefficiency were significantly related to the adolescents' functioning at all three observation points. High precipitating stress and short duration of symptoms (that is, in reactive illnesses) were associated with significantly better functioning at follow-up. Primary process thinking on the Rorschach was correlated with severe disturbances on admission, but was unrelated to outcome at discharge or follow-up. Maladaptive behavior on the unit was unrelated to functioning at any time. The authors concluded that outcome for hospitalized adolescents is determined by key patient differences and is systematically predictable.