Assuntos
Actinas/química , Produtos Biológicos/síntese química , Corantes Fluorescentes/química , Ácido Glutâmico/síntese química , Faloidina/síntese química , Actinas/metabolismo , Amanita/química , Ciclização , Corantes Fluorescentes/metabolismo , Ácido Glutâmico/análogos & derivados , Microscopia de Fluorescência , Modelos Químicos , Faloidina/análogos & derivados , Ligação ProteicaRESUMO
As part of our program to identify novel small molecules with interesting biological activity, we have designed and synthesized a library of end-capped dipeptides with an emphasis on compound diversity, complexity, and membrane permeability. An approximately 1500-member library was synthesized manually on large polystyrene beads using the mix-and-split method. The final compounds were cleaved into 384-well plates to generate individual stock solutions for input into high-throughput biological screens. Individual compounds were decoded using a combination of mass spectrometry and microflow NMR spectroscopy. In principle, this approach to deconvolution obviates the need for complicated binary encoding-decoding strategies for one-bead-one-compound libraries.