RESUMO
Trichomonas vaginalis infections are usually asymptomatic or can result in nonspecific clinical symptoms, which makes laboratory-based detection of this protozoan parasite essential for diagnosis and treatment. We report the development of a battery of highly sensitive and specific PCR assays for detection of T. vaginalis in urine, a noninvasive specimen, and development of a protocol for differentiating among Trichomonas species that commonly infect humans.
Assuntos
Técnicas de Laboratório Clínico/métodos , Técnicas de Diagnóstico Molecular/métodos , Parasitologia/métodos , Reação em Cadeia da Polimerase/métodos , Tricomoníase/diagnóstico , Trichomonas vaginalis/isolamento & purificação , Urina/parasitologia , Adolescente , Criança , Pré-Escolar , Feminino , Humanos , Lactente , Sensibilidade e Especificidade , Trichomonas vaginalis/classificação , Trichomonas vaginalis/genéticaRESUMO
To determine seropositivity for Enterocytozoon bieneusi in the Czech Republic, we tested 115 serum samples from various groups. We found that 20% from HIV-positive persons, 33% from persons with occupational exposure to animals, and 10% from healthy persons were positive by indirect immunofluorescence assay. Proteins of 32 kDa were detected in serum samples from seropositive persons.