Arbuscular Mycorrhizal Fungi Inducing Tomato Plant Resistance and Its Role in Control of Bemisia tabaci Under Greenhouse Conditions.

Arbuscular mycorrhizal fungi (AMF) are one of the environment-friendly organisms that enhance plant performance. AMF affect the herbivorous insect community by indirectly modifying host plant nutrient uptake, growth, and defense, also known as priming. In the current study, under greenhouse conditions, the effects of inoculating tomato seedlings with four species of AMF, i.e., Funneliformis mosseae, Rhizophagus intraradices, Rhizophagus irregularis, and Glomus iranicus, were studied in relation to tomato plant growth parameters, plant defense enzymes, and total phenol content, and additionally, the life table of Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) feeding on these plants was determined. The results demonstrated that the growth parameters of tomato plants, including plant height, stem diameter, number of leaves, root volume, leaf surface area, weight of the root, and aerial organs (containing the leaves and stem), were greater and larger in the AMF-inoculated plants compared to the non-inoculated plants. Furthermore, there were higher defense enzyme activities, including peroxidase, phenylalanine ammonia lyase and polyphenol oxidase, and also higher total phenol contents in the AMF-inoculated plants. The whitefly life table characteristics were decreased in the group feeding on the AMF-inoculated plants. All together, the AMF colonization made the tomato plants more resistant against B. tabaci by improving plant growth and increasing defense enzymes. The degree of priming observed here suggests the potential of AMF to have expansive applications, including their implementation in sustainable agriculture.

Anti-AChE and Anti-BuChE Screening of the Fermentation Broth Extracts from Twelve Aspergillus Isolates and GC-MS and Molecular Docking Studies of the Most Active Extracts.

Nowadays, the administration of cholinesterase enzyme (acetylcholinesterase: AChE and butyrylcholinesterase: BuChE) inhibitors is very common for the symptomatic treatment of Alzheimer's disease and the other forms of dementia and CNS disorders. In this paper, the anti-AChE and anti-BuChE activities of the fermentation broth ethyl acetate extracts from twelve Aspergillus isolates were evaluated by Ellman method. The results showed that A1 (Aspergillus flavus) and A5 (Aspergillus tubingensis, isolate 1) extracts with IC50 values of 46.77 µg/mL and 75.85 µg/mL possess the greatest ability to inhibit AChE and BuChE, respectively. GC-MS analysis of the extracts (A1 and A5) demonstrated that two alkaloids named 14-methyl-16-azabicyclo[10.3.1]hexadeca-1(15),12(16),13-triene (MAHT) and 6-chloro-2-methyl-7,8,9,10-tetrahydro-phenanthridine (CMTP) account for the highest percentage of A1 (26.95%) and A5 (25.5%) extracts, respectively. A 2-pyrazoline derivative, 5-hydroxy-3-(4-pyridinyl)-5-trifluoromethyl-1-(2,4,6-trimethylphenoxyacetyl)- (PHPTT), also constituted the high percentage (9.54%) of A5 extract. The anticholinesterase and neuroprotective effects of some 2-pyrazoline derivatives have been previously reported. The interaction study of MAHT with human AChE and CMTP and PHPTT with human BuChE using molecular docking indicated that these alkaloids bind to the active site gorge of the enzymes with high affinity. The best docking scores of MAHT, CMTP, and PHPTT were -7.1, -8.2, and -9.7 kcal/mol, respectively.

Species assignment and antifungal susceptibilities of black aspergilli recovered from otomycosis cases in Iran.

Black aspergilli are among the main causative agents of otomycosis worldwide. In this study, the species assignment of black aspergilli isolated from otomycosis cases in Iran was carried out using sequence analysis of part of the calmodulin gene. The results indicate that Aspergillus niger is not the only black Aspergillus species involved in otomycosis cases in Iran: Aspergillus awamori and Aspergillus tubingensis are also able to cause ear infections. Antifungal susceptibility tests were carried out against five antifungal drugs including amphotericin B, fluconazole, itraconazole, ketoconazole and terbinafine. All isolates were highly susceptible to terbinafine, while they exhibited moderate susceptibilities against amphotericin B, fluconazole and ketoconazole. Aspergillus niger and A. awamori were found to have higher minimal inhibitory concentrations for azoles than A. tubingensis, in accordance with previous findings.